Abstract：
:The subcellular distribution and substrate kinetics of soluble and particulate-associated bovine adrenal glycerol kinase have been investigated. Whole adrenal, adrenal cortex and adrenal medulla were examined for distribution of glycerol kinase between soluble and particulate fractions. No major differences in distribution were noted between these tissues; of the total homogenate activity, 0-20% sedimented with the nuclear fraction, 24-36% sedimented with the post-nuclear fraction and 64-69% remained soluble. Steady-state kinetic parameters of glycerol kinase activity were compared in the soluble and mitochondrial fractions. The Km for glycerol in the soluble fraction was 6.3 +/- 0.1 microM and in the mitochondrial fraction was 4.0 +/- 0.3 microM. The Km for ATP in soluble fraction was 12.8 +/- 1.5 and in the mitochondrial fraction was 5.3 +/- 1.6. Release of adrenal glycerol kinase from the mitochondrial fraction was investigated using inorganic phosphate, ATP and glycerol 3-phosphate. Of these compounds, only ATP and glycerol 3-phosphate were effective in releasing particulate-associated glycerol kinase. Inorganic phosphate had no effect upon release. Particulate-associated glycerol kinase activity of the mitochondrial fraction was stimulated by addition of succinate and ADP and was inhibited by addition of atractyloside. The data presented here indicate that bound glycerol kinase found within the mitochondrial fraction is kinetically distinct from soluble glycerol kinase and binding to mitochondria is responsive to substrate and product levels within the physiological range.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Seltzer WK,McCabe ERdoi
10.1007/BF00420928subject
Has Abstractpub_date
1984-09-01 00:00:00pages
51-61issue
1eissn
0300-8177issn
1573-4919journal_volume
64pub_type
杂志文章abstract：:A calcium activated photoprotein, termed mnemiopsin, which emits bioluminescence upon the addition of calcium ion, has been isolated from the Ctenophore, Memiopsis leidyi, and purified by hollow fiber techniques. The system is similar to aequorin, from the jellyfish Aequorea, except that mnemiopsin can be light-inacti...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00232600
更新日期：1978-04-11 00:00:00
abstract：:Pregna-D'-pentaranes (pentaranes) are modified progesterones with demonstrable progestational activity and contraceptive effect. We have examined the steroid binding characteristics of the two newly synthesized progesterone analogs, Pentarane A (16 alpha, 17 alpha-cyclohexanoprogesterone) and Pentarane B (6 alpha-meth...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00936444
更新日期：1993-08-25 00:00:00
abstract：:Stratifin is a member of 14-3-3 protein family, a highly conserved group of proteins constituted by seven isoforms. They are involved in numerous crucial intracellular functions such as cell cycle and apoptosis, regulation of signal transduction pathways, cellular trafficking, cell proliferation and differentiation, c...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s11010-007-9538-y
更新日期：2007-11-01 00:00:00
abstract：:Previous results with p9-RNA, obtained from lymph nodes of animals immunized with the peptide p9 of HIV-1, suggested that its effects on lymphocytes could be mediated by RNA-dependent protein kinase (PKR). Here we report that p9-RNA activates PKR leading to the degradation of the inhibitor I-kappaB alpha and the conco...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1024107512419
更新日期：2003-05-01 00:00:00
abstract：:Comparison of Ca2+ uptake by isolated mouse liver mitochondria, and mitochondria prepared from mastocytoma cells grown with and without N6,O2'-dibutyryladenosine 3',5' cyclic monophosphate (DB cyclic AMP) and theophylline showed several differences in their capacity to take up and retain calcium. In particular mitocho...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00215305
更新日期：1980-03-20 00:00:00
abstract：:The purpose of this study was to investigate the effect of metal-catalyzed oxidation by H(2)O(2) on the structure, oligomerization, and chaperone function of alphaA- and alphaB-crystallins. Recombinant alphaA-and alphaB-crystallins were prepared by expressing them in E. coli and purifying by size-exclusion chromatogra...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9128-4
更新日期：2006-08-01 00:00:00
abstract：:There is a striking interaction of genes and environment in the etiology of type 2 diabetes mellitus (T2DM). While endocrine disrupting chemicals (EDCs) like bisphenol-A (BPA) have received special attention for their mechanistic role in metabolic disruption, there is a lack of clinically relevant data on BPA levels i...
journal_title：Molecular and cellular biochemistry
pub_type： 临床试验,杂志文章
doi：10.1007/s11010-019-03540-9
更新日期：2019-08-01 00:00:00
abstract：:Guanidino compounds of guanidinoethanesulfonic acid, guanidinoacetic acid, guanidinosuccinic acid, N-acetylarginine, beta-guanidinopropionic acid, creatinine, gamma-guanidinobutyric acid, arginine, guanidine, methylguanidine, homoarginine and alpha-guanidinoglutaric acid are present in the mammalian brain. These guani...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：2003-02-01 00:00:00
abstract：:Acyl-CoA: lysolecithin and lysolecithin: lysolecithin acyltransferases, as well as acyl-CoA hydrolase are important enzymes in lung lipid metabolism. They use amphiphylic lipids as substrates and differ in subcellular localization. In this sense, lipid-protein interactions can be an essential factor in their activity....
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00214123
更新日期：1990-05-10 00:00:00
abstract：:This paper reviews the model of the control of mitochondrial substrate oxidation by Ca2+ ions. The mechanism is the activation by Ca2+ of four mitochondrial dehydrogenases, viz. glycerol 3-phosphate dehydrogenase, the pyruvate dehydrogenase multienzyme complex (PDH), NAD-linked isocitrate dehydrogenase (NAD-IDH) and 2...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Stress genes can be ascribed to have been generated by the organism for their intrinsic urge to survive against the changing environmental odds, during the evolutionary process. This concept has been supported by a large number of reports describing individual types of phenomena. These have been reconciled and globali...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1999-06-01 00:00:00
abstract：:MERRF (myoclonic epilepsy with ragged-red fibers) is a severe, multisystem disorder characterized by myoclonus, seizures, progressive cerebellar syndrome, muscle weakness, and the presence of ragged-red fibers in the muscle biopsy. MERRF is associated with heteroplasmic point mutations, either A8344G or T8356C, in the...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Bipolar disorder (BD) is a psychiatric disorder characterized by alternating episodes of mania and depression. The intracerebroventricular (i.c.v) administration of ouabain (a Na(+)/K(+)-ATPase inhibitor) in rats has been used as an animal model of mania, because present face, construct and predictive validities. Seve...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0455-0
更新日期：2010-08-01 00:00:00
abstract：:The Arrhenius plots for the active and low activity soluble forms of the ATPase purified from the membranes of Micrococcus lysodeikticus grown at 30 degrees C presented discontinuities at 30 and 33 degrees C, respectively. Their activation parameters differed, being highest for the low activity form of the enzyme. Bot...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01732550
更新日期：1977-08-19 00:00:00
abstract：:Pediatric cataract of the congenital type is the most common form of childhood blindness and it is clinically and genetically heterogeneous. Mutations in 22 different genes have been identified to be associated with congenital cataracts, and among them, eight mutants belong to αA-crystallin. To explain how mutations i...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-1131-8
更新日期：2012-03-01 00:00:00
abstract：:The action of 7.2 microM cardiotoxin on 0.25% human erythrocytes in a plasma extender solution was studied by the interaction of toxin with intact red blood cells and subsequent hemolysis of the cells. The binding of toxin to cells was completed within 10 min, whereas the membrane rigidity was weakened in a non-lytic ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00229378
更新日期：1987-01-01 00:00:00
abstract：:Recent studies have shown that allogeneic bone marrow (BM)-mesenchymal stem cell transplantation (MSCT) appears to be effective in systemic lupus erythematosus (SLE) patients and lupus-prone mice, contrary to studies in syngeneic BM-MSCT. These studies indicated that the abnormalities of BM-MSCs may be involved in the...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1866-5
更新日期：2014-02-01 00:00:00
abstract：:Glomerular mesangial cells play an important role in the development of glomerulosclerosis. Mesangial cell apoptosis has been shown to be involved in different stages of development of glomerulonephritis. The aim of the present study was to evaluate the effect of inhibition of serine/threonine phosphatases by okadaic ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/b:mcbi.0000026041.41078.32
更新日期：2004-05-01 00:00:00
abstract：:Acrolein is a highly reactive alpha, beta-unsaturated aldehyde, and a product of lipid peroxidation reactions. Acrolein is also an environmental pollutant and a key component of cigarette smoke, and has been implicated in multiple respiratory diseases. Lung tissue is a primary target for acrolein toxicity in smokers a...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-007-9411-z
更新日期：2007-07-01 00:00:00
abstract：:Extracts of fasted rat diaphragms, previously treated with or without insulin were assayed for glycogen synthase, protein kinase and cyclic [3H]-AMP binding. Treatment with insulin produced an elevation in the % of glycogen synthase I and a concurrent decrease in cyclic AMP-dependent protein kinase activity and cyclic...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00231232
更新日期：1978-02-24 00:00:00
abstract：:The effects of different dietary fats on peritoneal macrophage plasma membrane fluidity, intracellular cyclic AMP (cAMP) production, GTP hydrolysis and TNF binding and TNF-induced IL1 and IL6 production was investigated. After a four week period, fluidity, as determined by both fluorescence recovery after photobleachi...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006875010120
更新日期：1997-01-01 00:00:00
abstract：:We have investigated how diabetes affects the expression of adropin (ADR) in rat brain, cerebellum, kidneys, heart, liver, and pancreas tissues. The rats in the diabetic group were administered an intraperitoneal (i.p.) injection of a single dose of 60 mg/kg streptozotocin (STZ) dissolved in a 0.1 M phosphate-citrate ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1660-4
更新日期：2013-08-01 00:00:00
abstract：:Endothelial cell nitric oxide synthase (NOS) is known to have a N-myristoylation consensus sequence. Such a consensus sequence is not evident in the macrophage, smooth muscle and neuronal NOS. A functional role for this N-terminal myristoylation is not clear yet. In the present study, we examined the effect of N-termi...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01076076
更新日期：1995-11-22 00:00:00
abstract：:In this study, the effect of (Boc-Lys (Boc)-Arg-Asp-Ser (tBu)-OtBu), a tetrapeptide derivative (PEP1261) was examined for antiproliferative potency and apoptotic induction. Synovial fibroblasts were isolated from collagen-induced arthritic (CIA) rats and exposed to peptides viz., PEP1261, and parental peptides (KRDS a...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-1737-4
更新日期：2006-01-01 00:00:00
abstract：:Both reactive oxygen- and nitrogen-derived reactive species play important roles in physiological and pathophysiological conditions. Flavones, luteolin and luteolin-7-O-glucoside along with a rich plant source of both flavones, namely dandelion (Taraxacum officinale) flower extract were studied for antioxidant activit...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/b:mcbi.0000044364.73144.fe
更新日期：2004-10-01 00:00:00
abstract：:During fetal life, myocardial ATP is derived predominantly from glycolysis and lactate oxidation. Following birth, a rapid maturational increase in fatty acid oxidation occurs along with a decline in glycolytic and lactate oxidative rates, thus changing the major source of myocardial ATP production. This shift in ener...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
abstract：:This study investigated the anticancer effects of embelin in human gastric cancer cells and the underlying molecular mechanisms. Gastric cancer cells were treated with embelin and 5-FU for methyl-thiazolyl-tetrazolium bromide cell viability assay and flow cytometric detection of cell viability and apoptosis. Protein p...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1853-x
更新日期：2014-01-01 00:00:00
abstract：:Cross-talk between coding RNAs and regulatory non-coding microRNAs, within human genome, has provided compelling evidence for the existence of flexible checkpoint control of T-Cell activation. The present study attempts to demonstrate that the interplay between miR-2909 and its effector KLF4 gene has the inherent capa...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-018-3390-0
更新日期：2019-01-01 00:00:00
abstract：:Physical training induces cardiovascular autonomic nervous system regulation adaptations, which could result from beta adrenergic receptor (AR) modifications. Among them, beta(3 )AR alterations have been recently reported but their functional effect remained to discuss. To explain the beta(3) AR gene expression in rel...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-006-9370-9
更新日期：2007-06-01 00:00:00
abstract：:The regulation of the guinea-pig pancreatic acinar plasma membrane Ca2+ pump by protein kinase A, protein kinase C and calmodulin was investigated. The results were compared with the effects of these regulators on the high affinity Ca(2+)-ATPase found in this membrane preparation. The catalytic subunit of cyclic AMP-d...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00227572
更新日期：1992-06-26 00:00:00
