当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOCHEMISTRY期刊下所有文献 > Effect of oxidation of alphaA- and alphaB-crystallins on their structure, oligomerization and chaperone function.

Effect of oxidation of alphaA- and alphaB-crystallins on their structure, oligomerization and chaperone function.

Abstract：

:The purpose of this study was to investigate the effect of metal-catalyzed oxidation by H(2)O(2) on the structure, oligomerization, and chaperone function of alphaA- and alphaB-crystallins. Recombinant alphaA-and alphaB-crystallins were prepared by expressing them in E. coli and purifying by size-exclusion chromatography. They were incubated with 1.5 mM H(2)O(2) and 0.1 mM FeCl(3) at 37 ( composite function)C for 24 hrs and the reaction was stopped by adding catalase. Structural changes due to oxidation were ascertained by circular dichroism (CD) measurements and chaperone activity was assayed with alcohol dehydrogenase (ADH) and insulin as target proteins. The oligomeric nature of the oxidized proteins was assessed by molecular sieve HPLC. The secondary structure of the oxidized alphaA- and alphaB-crystallins has been substantially altered due to significant increase in random coils, in addition to decrease in beta-sheet or alpha-helix contents. The tertiary structure also showed significant changes indicative of different mode of folding of the secondary structural elements. Chaperone function was significantly compromised as supported by nearly 50% loss in chaperone activity. Oxidation also resulted in the formation of higher molecular weight (HMW) proteins as well as lower molecular weight (LMW) proteins. Thus, oxidation leads to disintegration of the oligomeric structure of alphaA- and alphaB-crystallins. Chaperone activity of the HMW fraction is normal whereas the LMW fraction lacks any chaperone activity. So, it appears that the formation of the LMW proteins is the primary cause of the chaperone activity loss due to oxidation.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Rajan S,Horn C,Abraham EC

doi

10.1007/s11010-006-9128-4

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

125-34

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

288

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOCHEMISTRY文献大全
  • Clinical significance of cathepsin L and cathepsin B in dilated cardiomyopathy.

    abstract：:Dysregulated expression of lysosomal cysteine cathepsins is associated with adverse cardiac remodeling, a characteristic of several cardiovascular diseases. However, the information regarding the role of cysteine cathepsin L (CTSL) and cathepsin B (CTSB) in dilated cardiomyopathy (DCM) is limited. The present study wa...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-016-2924-6

    authors： Mehra S,Kumar M,Manchanda M,Singh R,Thakur B,Rani N,Arava S,Narang R,Arya DS,Chauhan SS

    更新日期：2017-04-01 00:00:00

  • Evidence that glycine induces lipid peroxidation and decreases glutathione concentrations in rat cerebellum.

    abstract：:Patients with non-ketotic hyperglycinemia (NKH) present severe neurological symptoms and brain abnormalities involving cerebellum. Although the pathomechanisms underlying the cerebellum damage have not been studied, high tissue levels of glycine (GLY), the biochemical hallmark of this disorder have been suggested to c...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2118-z

    authors： Moura AP,Grings M,Marcowich GF,Bumbel AP,Parmeggiani B,de Moura Alvorcem L,Wajner M,Leipnitz G

    更新日期：2014-10-01 00:00:00

  • Perifosine as potential anti-cancer agent inhibits proliferation, migration, and tube formation of human umbilical vein endothelial cells.

    abstract：:Targeting angiogenesis is considered an effective strategy for treating the expansion and metastasis of tumors. The aim of this study is to assess the effects of perifosine, an inhibitor of Akt, on cell proliferation, apoptosis, angiogenesis, and VEGF-induced cell migration in cultured human umbilical vein endothelial...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-0986-z

    authors： Wang FZ,Fei HR,Li XQ,Shi R,Wang de C

    更新日期：2012-09-01 00:00:00

  • Angiotensin converting enzyme inhibitors, left ventricular hypertrophy and fibrosis.

    abstract：:From pharmacological investigations and clinical studies, it is known that angiotensin converting enzyme (ACE) inhibitors exhibit additional local actions, which are not related to hemodynamic changes and which cannot be explained only by interference with the renin angiotensin system (RAS) by means of an inhibition o...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/BF00944788

    authors： Linz W,Wiemer G,Schaper J,Zimmermann R,Nagasawa K,Gohlke P,Unger T,Schölkens BA

    更新日期：1995-06-07 00:00:00

  • Plasminogen activator activity in differentiating rat myoblasts.

    abstract：:Primary cultures of skeletal muscle cells secrete plasminogen activator (PA) activity to the conditioning medium and display membrane-bound PA. Growth of these cells in culture in presence of 10(-7) M dexamethasone resulted in a marked reduction of the membranal and secreted PA activity. The hormone also reduced cytos...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00224761

    authors： Mayer M,Finci Z,Chaouat M,Sasson S

    更新日期：1986-02-01 00:00:00

  • Mechanism of attenuation by beta-hydroxy-beta-methylbutyrate of muscle protein degradation induced by lipopolysaccharide.

    abstract：:The mechanism of the effect of beta-hydroxy-beta-methylbutyrate (HMB) on protein degradation induced by lipopolysaccharide (LPS) has been evaluated in murine myotubes. HMB (50 muM) completely attenuated total protein degradation induced by LPS (1-100 ng/ml), formation of reactive oxygen species (ROS) and activation of...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-009-0130-5

    authors： Russell ST,Tisdale MJ

    更新日期：2009-10-01 00:00:00

  • Energy metabolism and mechanical recovery after cardioplegia in moderately hypertrophied rats.

    abstract：:It is well established that severe hypertrophy induces metabolic and structural changes in the heart which result in enhanced susceptibility to ischemic damage during cardioplegic arrest while much less is known about the effect of cardioplegic arrest on moderately hypertrophied hearts. The aim of this study was to el...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：

    authors： Smolenski RT,Jayakumar J,Seymour AM,Yacoub MH

    更新日期：1998-03-01 00:00:00

  • CK2 phosphorylation of SAG at Thr10 regulates SAG stability, but not its E3 ligase activity.

    abstract：:Sensitive to Apoptosis Gene (SAG), a RING component of SCF E3 ubiquitin ligase, was shown to be phosphorylated by protein kinase CK2 at the Thr10 residue. It is, however, unknown whether this phosphorylation is stress-responsive or whether the phosphorylation changes its E3 ubiquitin ligase activity. To address these,...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-006-9287-3

    authors： He H,Tan M,Pamarthy D,Wang G,Ahmed K,Sun Y

    更新日期：2007-01-01 00:00:00

  • Carnosic acid promotes myocardial antioxidant response and prevents isoproterenol-induced myocardial oxidative stress and apoptosis in mice.

    abstract：UNLABELLED:Carnosic acid is a well-known antioxidant. Recently, it has been identified as modulator of nuclear factor erythroid 2-related factor 2 (Nrf2). The effect of carnosic acid in the context of cardiovascular disorders has not been studied. In the present study, we investigated the beneficial effect and the unde...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2092-5

    authors： Sahu BD,Putcha UK,Kuncha M,Rachamalla SS,Sistla R

    更新日期：2014-09-01 00:00:00

  • L-tyrosine: 2-oxoglutarate aminotransferase induction by hydrocortisone in the thymus of the white rat.

    abstract：:Hydrocortisone hemisuccinate within 4 hours after in vivo administration produced an increase in precursor incoporation into rat thymus RNA and proteins in the whole animal. From these results, together with information obtained from measurements of the tyrosine aminotransferase activity and the action of mitomycin C ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF01751307

    authors： Abraham AD

    更新日期：1975-12-31 00:00:00

  • Functional analysis of pig myostatin gene promoter with some adipogenesis- and myogenesis-related factors.

    abstract：:Myostatin (MSTN) is primarily expressed in muscle and plays an important role in muscle and fat development in pigs. However, there is little information about the regulation of pig MSTN. In order to elucidate whether pig MSTN could be regulated by muscle- and fat-related factors, the porcine MSTN promoter was amplifi...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-011-1181-y

    authors： Deng B,Wen J,Ding Y,Gao Q,Huang H,Ran Z,Qian Y,Peng J,Jiang S

    更新日期：2012-04-01 00:00:00

  • The roles of NLRP3 inflammasome-mediated signaling pathways in hyperuricemic nephropathy.

    abstract：:Hyperuricemic nephropathy (HN) is a common clinical complication of hyperuricemia. High-serum uric acid can trigger renal inflammation. The inflammasome family has several members and shows a significant effect on inflammatory responses. NLRP3 (NOD-, LRR-, and pyrin domain-containing 3) senses the stimuli signal of ex...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：10.1007/s11010-020-03997-z

    authors： Wen L,Yang H,Ma L,Fu P

    更新日期：2021-01-03 00:00:00

  • Fuel smoke condensate induced DNA damage in human lymphocytes and protection by turmeric (Curcuma longa).

    abstract：:Twigs-dry leaves smoke condensate (TDS) was investigated for its DNA damaging activity in human peripheral lymphocytes, by using a sensitive method, fluorescence analysis of DNA unwinding (FADU). An aqueous turmeric component (Aq.T) was studied as a protective agent. TDS at one to 100 folds dilution induced 55% DNA da...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00219526

    authors： Shalini VK,Srinivas L

    更新日期：1990-06-01 00:00:00

  • Alterations in cardiac function and subcellular membrane activities after hypervitaminosis D3.

    abstract：:The present study was designed to induce massive accumulation of calcium in the myocardium and to evaluate the effect of calcium overload on myocardial contractile function and biochemical activity of cardiac subcellular membranes. Rats were treated with an oral administration of 500,000 units/kg of vitamin D3 for 3 c...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00225520

    authors： Takeo S,Tanonaka R,Tanonaka K,Miyake K,Hisayama H,Ueda N,Kawakami K,Tsumura H,Katsushika S,Taniguchi Y

    更新日期：1991-10-16 00:00:00

  • Proteolysis activated protein kinase in Dictyostelium discoideum.

    abstract：:In the search for MBP phosphorylating activities in Dictyostelium discoideum, we have found a proteolysis-activated protein kinase. This activity which is distributed between the soluble and the particulate fractions of the cell, uses MBP and histone as substrate and has a molecular mass of 140 kDa as detected in an '...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1006809202539

    authors： Nuñez A,Fernández-Renart M

    更新日期：1997-10-01 00:00:00

  • Up-regulation of interferon-stimulated gene15 and its conjugates by tumor necrosis factor-α via type I interferon-dependent and -independent pathways.

    abstract：:Interferon-stimulated gene15 (ISG15) is the first characterized ubiquitin-like protein, which is strongly induced by type I interferons (IFN-α/β), bacterial endotoxin, and cellular stress. Up-regulation of ISG15 is observed in several cancer cell types and is associated with cancer progression. As many cytokines can i...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1360-5

    authors： Chairatvit K,Wongnoppavich A,Choonate S

    更新日期：2012-09-01 00:00:00

  • Splicing factors differentially expressed in psoriasis alter mRNA maturation of disease-associated EDA+ fibronectin.

    abstract：:The EDA+ fibronectin splicing variant is overexpressed in psoriatic non-lesional epidermis and sensitizes keratinocytes to mitogenic signals. However, regulation of its abundance is only partially understood. In our recent cDNA microarray experiment, we identified three SR-rich splicing factors-splicing factor, argini...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-017-3090-1

    authors： Szlavicz E,Szabo K,Groma G,Bata-Csorgo Z,Pagani F,Kemeny L,Szell M

    更新日期：2017-12-01 00:00:00

  • The Translocon-Associated Protein beta (TRAPbeta) in zebrafish embryogenesis. I. Enhanced expression of transcripts in notochord and hatching gland precursors.

    abstract：:The normal translocation of nascent polypeptides into the lumen of the endoplasmic reticulum (ER) is thought to be aided in part by a translocon-associated protein (TRAP) complex consisting of 4 protein subunits. The association of mature proteins with the ER and Golgi, or other intracellular locales, such as lysosome...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1026598516681

    authors： Mangos S,Krawetz R,Kelly GM

    更新日期：2000-12-01 00:00:00

  • The interrelationship of somatostatin and guanylate cyclase activity.

    abstract：:Somatostatin has been shown to inhibit the release of various polypeptide hormones including insulin, glucagon, gastrin, thyroid stimulating hormone, and growth hormone. The mechanism by which somatostatin inhibits the release of these various polypeptide hormones has not been fully elucidated. It has been reported th...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00227439

    authors： Vesely DL

    更新日期：1980-11-20 00:00:00

  • Liver-specific ZP domain-containing protein (LZP) as a new partner of Tamm-Horsfall protein harbors on renal tubules.

    abstract：:Liver-specific ZP domain-containing protein (LZP) was recently identified as a secreted protein that is specifically expressed in liver. However, the physiological effects of LZP are largely unknown. In this study, we found that LZP was detectable in mouse kidneys, testes, ovaries and heart, in addition to liver. LZP ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-008-9921-3

    authors： Shen HL,Xu ZG,Huang LY,Liu D,Lin DH,Cao JB,Zhang X,Wang ZQ,Wang WH,Yang PY,Han ZG

    更新日期：2009-01-01 00:00:00

  • The m6A methyltransferase METTL3 cooperates with demethylase ALKBH5 to regulate osteogenic differentiation through NF-κB signaling.

    abstract：:As a m6A methylation modifier, METTL3 is functionally involved in various biological processes. Nevertheless, the role of METTL3 in osteogenesis is not determined up to date. In the current study, METTL3 is identified as a crucial regulator in the progression of osteogenic differentiation. Loss of METTL3 significantly...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-019-03641-5

    authors： Yu J,Shen L,Liu Y,Ming H,Zhu X,Chu M,Lin J

    更新日期：2020-01-01 00:00:00

  • Inhibitory effect of Styrax Japonica Siebold et al. Zuccarini glycoprotein (38 kDa) on interleukin-1β and induction proteins in chromium(VI)-treated BNL CL.2 cells.

    abstract：:Chromium(VI) [Cr(VI)] induces chronic inflammation in hepatocytes. Inflammation has been shown to play an important role in tumorigenesis, tumor progression, and metastasis. To examine the effects of the Styrax Japonica Siebold et al. Zuccarini (SJSZ) glycoprotein on inflammation in BNL CL.2 cells, we evaluated the ac...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-012-1324-9

    authors： Lee J,Lim KT

    更新日期：2012-08-01 00:00:00

  • Post-receptor modulation of the effects of cyclic AMP in isolated cardiac myocytes.

    abstract：:The actions of cyclic AMP are subject to several levels of post-receptor modulation in cardiac tissue. Isoproterenol and prostaglandin E1 both stimulate cAMP accumulation, but only isoproterenol causes activation of particulate cAMP-dependent protein kinase, leading to activation of phosphorylase kinase and glycogen p...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00242510

    authors： Bode DC,Brunton LL

    更新日期：1988-07-01 00:00:00

  • Phosphoinositide 3-kinase activity leads to silica-induced NF-kappaB activation through interacting with tyrosine-phosphorylated I(kappa)B-alpha and contributing to tyrosine phosphorylation of p65 NF-kappaB.

    abstract：:The role of the subunits of phosphoinositide (PI) 3-kinase in NF-kappaB activation in silica-stimulated RAW 264.7 cells was investigated. Results indicate that PI3-kinase activity was increased in response to silica. The p85alpha subunit of PI3-kinase interacted with tyrosine-phosphorylated I(kappa)B-alpha in silica-s...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1024163630166

    authors： Kang JL,Lee HS,Pack IS,Hur KC,Castranova V

    更新日期：2003-06-01 00:00:00

  • Effect of superoxide dismutase on early radiation injury of lungs in the rat.

    abstract：:Early responses of lungs to a single radiation dose of 30 Gy were marked by an inflammatory reaction and the onset of pneumonitis within 4 weeks following hemithorax radiation in the rat. Superoxide dismutase reduced the severity of radiation lesions in lungs. ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00421048

    authors： Malaker K,Das RM

    更新日期：1988-12-01 00:00:00

  • A role for guanidino compounds in the brain.

    abstract：:Guanidino compounds of guanidinoethanesulfonic acid, guanidinoacetic acid, guanidinosuccinic acid, N-acetylarginine, beta-guanidinopropionic acid, creatinine, gamma-guanidinobutyric acid, arginine, guanidine, methylguanidine, homoarginine and alpha-guanidinoglutaric acid are present in the mammalian brain. These guani...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章,评审

    doi：

    authors： Hiramatsu M

    更新日期：2003-02-01 00:00:00

  • Effect of albumin on acyl-CoA: lysolecithin acyltransferase, lysolecithin: lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung.

    abstract：:Acyl-CoA: lysolecithin and lysolecithin: lysolecithin acyltransferases, as well as acyl-CoA hydrolase are important enzymes in lung lipid metabolism. They use amphiphylic lipids as substrates and differ in subcellular localization. In this sense, lipid-protein interactions can be an essential factor in their activity....

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/BF00214123

    authors： Pérez-Gil J,Estrada P,Acebal C,Arche R

    更新日期：1990-05-10 00:00:00

  • Role of SIRT1 in Streptococcus pneumoniae-induced human β-defensin-2 and interleukin-8 expression in A549 cell.

    abstract：:Streptococcus pneumoniae is an important pathogen of pneumonia in human. Human alveolar epithelium acts as an effective barrier and is an active participant in host defense against invasion of bacterial by production of various mediators. Sirtuin 1 (SIRT1), the prototypic class III histone deacetylase, is involved in ...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-014-2095-2

    authors： Lin L,Wen SH,Guo SZ,Su XY,Wu HJ,Chong L,Zhang HL,Zhang WX,Li CC

    更新日期：2014-09-01 00:00:00

  • Experimental myocardial necrosis in rats: role of arjunolic acid on platelet aggregation, coagulation and antioxidant status.

    abstract：:Arjunolic acid, a new triterpene and a potent principle from the bark of Terminalia arjuna, has been shown to provide significant cardiac protection in isoproterenol induced myocardial necrosis in rats. To further explore the mechanism of action of arjunolic acid, antiplatelet activity, anticoagulant assays, electroca...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1023/a:1011927812753

    authors： Sumitra M,Manikandan P,Kumar DA,Arutselvan N,Balakrishna K,Manohar BM,Puvanakrishnan R

    更新日期：2001-08-01 00:00:00

  • Experimental evidence for the therapeutic potential of tempol in the treatment of acute liver injury.

    abstract：:Oxidative stress is one of the mechanisms involved in the acute carbon tetrachloride (CCl4)-induced hepatotoxicity. Since 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl, known as tempol, has powerful antioxidant properties, we investigated its potential hepatoprotective effects and the underlying mechanisms that may ad...

    journal_title：Molecular and cellular biochemistry

    pub_type： 杂志文章

    doi：10.1007/s11010-015-2572-2

    authors： Abouzied MM,Eltahir HM,Taye A,Abdelrahman MS

    更新日期：2016-01-01 00:00:00