Measurement of nucleotide pools in platelets using high pressure liquid chromatography.

abstract：:Plasminogen activator inhibitor 1 (PAI-1) is the main inhibitor of tissue-type and urokinase-type plasminogen activators (t/uPA) and plays an important role in fibrinolysis. Inhibition of PAI-1 activity prevents thrombosis and accelerates fibrinolysis, indicating that PAI-1 inhibitors may be used as effective antithro...

journal_title：Thrombosis and haemostasis

authors： Peng S,Xue G,Gong L,Fang C,Chen J,Yuan C,Chen Z,Yao L,Furie B,Huang M

更新日期：2017-06-28 00:00:00

abstract：:Venous thromboembolism (VTE) is a central concern in the intensive care unit (ICU). However, little is known about both current practices for VTE prevention in the ICU and the risk for VTE in persons with severe sepsis and septic shock. XPRESS was a randomized, double-blind, placebo-controlled trial of prophylactic he...

journal_title：Thrombosis and haemostasis

authors： Shorr AF,Williams MD

更新日期：2009-01-01 00:00:00

abstract：:Recombinant activated factor VII (rFVIIa; NovoSeven®) is a heterogeneously glycosylated serine protease used for treatment of haemophiliacs with inhibitors. The drug substance contains a subpopulation consisting of ~20% of rFVIIa molecules which are unsialylated and consists of carbohydrate moieties with terminally ex...

journal_title：Thrombosis and haemostasis

authors： Seested T,Nielsen HM,Christensen EI,Appa RS

更新日期：2010-12-01 00:00:00

abstract：:In vitro, concentration-dependent effects of rt-PA on a range of coagulation and fibrinolytic assays in thawed plasma samples were investigated. In absence of a fibrinolytic inhibitor, 2 micrograms rt-PA/ml blood (3.4 micrograms/ml plasma) caused prolongation of clotting time assays and decreases of plasminogen (to 44...

journal_title：Thrombosis and haemostasis

authors： Seifried E,Tanswell P

更新日期：1987-10-28 00:00:00

abstract：:Platelet-rich clots are inefficiently lysed by current fibrinolytic agents. Platelets contain a great deal of plasminogen activator inhibitor 1 (PAI-1), the principal endogenous inhibitor of tissue-type plasminogen activator (t-PA). We have tested whether PAI-1 resistant t-PAs would be more effective thrombolytic agen...

journal_title：Thrombosis and haemostasis

authors： Shohet RV,Spitzer S,Madison EL,Bassel-Duby R,Gething MJ,Sambrook JF

更新日期：1994-01-01 00:00:00

abstract：:Thromboembolic complications have been attributed to the use of radiographic contrast media (CM) during interventional procedures for arterial revascularization. However, due to the low frequency of adverse events, comparisons between different CM have been difficult to perform, although it has been suggested that ion...

journal_title：Thrombosis and haemostasis

authors： Markou CP,Chronos NA,Hanson SR

更新日期：2001-03-01 00:00:00

abstract：:Platelet activation as a result of vascular injury provokes endothelial cells to respond in a manner which limits or reverses the occlusive consequences of platelet accumulation. If the agonistic forces are strong, platelet accumulation is irreversible. In vitro data from our laboratory have repeatedly demonstrated th...

journal_title：Thrombosis and haemostasis

authors： Marcus AJ,Safier LB,Broekman MJ,Islam N,Fliessbach JH,Hajjar KA,Kaminski WE,Jendraschak E,Silverstein RL,von Schacky C

更新日期：1995-07-01 00:00:00

abstract：:Tissue factor (thromboplastin or Factor III), a glycoprotein cofactor, is required for Factor VII to express its catalytic activity, thereby initiating the extrinsic as well as intrinsic pathway of blood coagulation. Human brain tissue factor was purified 2,500-fold to 98% homogeneity from 2% Triton X-100 extraction o...

journal_title：Thrombosis and haemostasis

authors： Kang S,Niemetz J

更新日期：1988-06-16 00:00:00

abstract：:It was the aim of the present study to perform a systematic review of the published studies that estimated the prevalence of non-responders to aspirin, as assessed by the closure time of PFA-100, a point-of-care device, and to analyse: 1) some major clinical and methodological factors that can influence it and 2) its ...

journal_title：Thrombosis and haemostasis

authors： Crescente M,Di Castelnuovo A,Iacoviello L,Vermylen J,Cerletti C,de Gaetano G

更新日期：2008-01-01 00:00:00

abstract：:DX-9065a is an antithrombin III (AT III)-independent and selective inhibitor of activated blood coagulation factor X (FXa). We evaluated the effects of DX-9065a and warfarin on bleeding time and blood loss in rat tail transection model and on blood loss in hydrochloride (HCl)-induced rat gastrointestinal haemorrhage m...

journal_title：Thrombosis and haemostasis

authors： Tanabe K,Morishima Y,Shibutani T,Terada Y,Hara T,Shinohara Y,Aoyagi K,Kunitada S,Kondo T

更新日期：1999-05-01 00:00:00

abstract：:Coumarin and homologous compounds are the most widely used anticoagulant drugs worldwide. They function as antagonists of vitamin K, an essential cofactor for the posttranslational gamma-glutamyl carboxylation of the so-called vitamin K-dependent proteins. As vitamin K hydroquinone is converted to vitamin K epoxide (V...

journal_title：Thrombosis and haemostasis

authors： Rost S,Fregin A,Hünerberg M,Bevans CG,Müller CR,Oldenburg J

更新日期：2005-10-01 00:00:00

abstract：:In the last decade, several studies revealed inter-patient response variability to antiplatelet agents: patients who display negligible or no responses to these drugs are considered poor responders, or "resistant" to treatment. In order to identify poor responders to an antiplatelet drug, laboratory tests of platelet ...

journal_title：Thrombosis and haemostasis

authors： Cattaneo M

更新日期：2013-05-01 00:00:00

abstract：:We have identified a patient with a dysfunctional prothrombin that we have designated Prothrombin Frankfurt. The proband was characterized by a prothrombin activity level of 13% and 20% compared to normal controls using two different assays with a normal prothrombin antigen level of 91% of normal controls. The genetic...

journal_title：Thrombosis and haemostasis

authors： Degen SJ,McDowell SA,Sparks LM,Scharrer I

更新日期：1995-02-01 00:00:00

abstract：:Severe type I plasminogen deficiency is the underlying cause of ligneous conjunctivitis, a rare disease characterized by wood-like pseudomembranes developing on the ocular and extraocular mucosa. Two unrelated female patients with ligneous conjunctivitis and moderate hypoplasminogenemia are described. Being of fertile...

journal_title：Thrombosis and haemostasis

authors： Teresa Sartori M,Saggiorato G,Pellati D,Casonato A,Spiezia L,Pontara E,Gavasso S,Girolami A

更新日期：2003-07-01 00:00:00

abstract：:Wound healing is a complicated biological process that consist of partially overlapping inflammatory, proliferation and tissue remodelling phases. A successful wound healing depends on a proper activation and subsequent termination of the inflammatory phase. The failure to terminate the inflammation halts the completi...

journal_title：Thrombosis and haemostasis

authors： Sulniute R,Shen Y,Guo YZ,Fallah M,Ahlskog N,Ny L,Rakhimova O,Broden J,Boija H,Moghaddam A,Li J,Wilczynska M,Ny T

更新日期：2016-05-02 00:00:00

abstract：:Recently we prepared sulfated, low-molecular-weight lignins (LMWLs) to mimic the biological activities of heparin and heparan sulfate. Chemo-enzymatically prepared sulfated LMWLs represent a library of diverse non-sugar, aromatic molecules with structures radically different from the heparins, and have been found to p...

journal_title：Thrombosis and haemostasis

authors： Henry BL,Abdel Aziz M,Zhou Q,Desai UR

更新日期：2010-03-01 00:00:00

abstract：:We have established a new, enzyme-linked immunosorbent assay (ELISA) for the detection of ADAMTS13 antigen using purified polyclonal rabbit anti-human ADAMTS13 IgG. Normal plasma ADAMTS13 antigen levels span a concentration of 740-1420 ng/ml (median 1080 ng/ml) resulting in an ADAMTS13 activity to antigen ratio of 0.4...

journal_title：Thrombosis and haemostasis

authors： Rieger M,Ferrari S,Kremer Hovinga JA,Konetschny C,Herzog A,Koller L,Weber A,Remuzzi G,Dockal M,Plaimauer B,Scheiflinger F

更新日期：2006-02-01 00:00:00

abstract：:Findings from a recent study suggest that earlier start of treatment with factor VIII in patients with severe hemophilia is associated with a higher risk to develop inhibitors. We set out to assess the association between age of first administration of clotting factor VIII and the risk to develop inhibitors in infants...

journal_title：Thrombosis and haemostasis

authors： van der Bom JG,Mauser-Bunschoten EP,Fischer K,van den Berg HM

更新日期：2003-03-01 00:00:00

abstract：:The urinary excretion rate of antithrombin III related antigen (AT III RA) was examined in cerebral stroke. The excretion rate of AT III RA in cerebral hemorrhage (CH) was 12.33 +/- 1.61 X 10(-4) ml/min. The patients with CH were further classified into two groups: in group CH-I, whose consciousness state was stupor o...

journal_title：Thrombosis and haemostasis

authors： Matsuo T,Shinomiya K,Okuno S,Matsuo C,Matsuo O

更新日期：1984-12-29 00:00:00

abstract：:The difficulties in cardiovascular drug development have been exposed by recent clinical trials, which have uncovered various limitations of promising drug candidates. Yet, the imperative to improve medical treatment of atherosclerosis in aging populations afflicted by metabolic disease remains unbroken. Herein altern...

journal_title：Thrombosis and haemostasis

authors： Weber C

更新日期：2010-07-01 00:00:00

abstract：:In 70-80% of cases, pulmonary embolism is the consequence of lower extremity deep vein thrombosis. It has been demonstrated that the most common coagulation defect predisposing to venous thrombosis, resistance to activated protein C (APC), is not associated with an increased risk for pulmonary embolism, but the eviden...

journal_title：Thrombosis and haemostasis

authors： Martinelli I,Cattaneo M,Panzeri D,Mannucci PM

更新日期：1997-03-01 00:00:00

abstract：:Diabetic subjects have been shown to have altered fibrin network structures. One possible cause may be fibrinogen glycation resulting in altered structure/function properties. We investigated the effect of glucose control on fibrinogen glycation and fibrin network structure in type 2 diabetes. Blood samples were taken...

journal_title：Thrombosis and haemostasis

authors： Pieters M,Covic N,Loots du T,van der Westhuizen FH,van Zyl DG,Rheeder P,Jerling JC,Weisel JW

更新日期：2006-11-01 00:00:00

abstract：:Charge-to-alanine mutations of three amino acid residues, viz, D46, D48, and D/Hya71, which are known to be important in stabilizing Ca2+ binding to epidermal growth factor (EGF) domains of vitamin K-dependent blood coagulation proteins, have been engineered into recombinant human protein C (r-PC). The resulting varia...

journal_title：Thrombosis and haemostasis

authors： Geng JP,Cheng CH,Castellino FJ

更新日期：1996-11-01 00:00:00

abstract：:Novel strategies for a tailored risk prediction in chronic heart failure (CHF) are crucial to identify patients at very high risk for an improved patient management and to specify treatment regimens. Endothelial progenitor cells (EPCs) are an important endogenous repair mechanism with the ability to counteract endothe...

journal_title：Thrombosis and haemostasis

authors： Koller L,Hohensinner P,Sulzgruber P,Blum S,Maurer G,Wojta J,Hülsmann M,Niessner A

更新日期：2016-08-01 00:00:00

abstract：:Direct oral anticoagulants may be effective and safe for treatment of venous thromboembolism (VTE) in cancer patients, but they have not been compared with low-molecular-weight heparin (LMWH), the current recommended treatment for these patients. The Hokusai VTE-cancer study is a randomised, open-label, clinical trial...

journal_title：Thrombosis and haemostasis

authors： van Es N,Di Nisio M,Bleker SM,Segers A,Mercuri MF,Schwocho L,Kakkar A,Weitz JI,Beyer-Westendorf J,Boda Z,Carrier M,Chlumsky J,Décousus H,Garcia D,Gibbs H,Kamphuisen PW,Monreal M,Ockelford P,Pabinger I,Verhamme P,G

更新日期：2015-11-25 00:00:00

abstract：:The proposed 2nd International Standard for Factor VIII and von Willebrand Factor activities in plasma, NIBSC code 87/718, was assayed against the 1st IRP, 80/511, and against fresh normal plasma, in 21 laboratories. There were no significant differences between the various assay methods for factor VIII antigen, von W...

journal_title：Thrombosis and haemostasis

authors： Heath AB,Barrowcliffe TW

更新日期：1992-08-03 00:00:00

abstract：:A fibrinolysis inhibitor was purified in serum from post-traumatic patients by the use of flat bed electrofocusing of serum desalted by gel chromatography followed by affinity chromatography on a column of matrix-linked plasminogen. Disc gel electrophoresis yielded one protein band. The inhibitor protein was also foun...

journal_title：Thrombosis and haemostasis

authors： Bagge L,Björk I,Saldeen T,Wallin R

更新日期：1978-02-28 00:00:00

abstract：:Thrombospondin, a trimeric glycoprotein contained in the platelet alpha-granules, has been proposed as a marker of in vivo platelet activation. However, it is also synthesised by a range of other cells. The extraplatelet contribution to plasma levels of thrombospondin was therefore estimated by investigating the relat...

journal_title：Thrombosis and haemostasis

authors： Dawes J,Pratt DA,Dewar MS,Preston FE

更新日期：1988-04-08 00:00:00

abstract：:Platelet-monocyte aggregates are increasingly being used to quantify platelet activation. The variables that influence platelet-monocyte aggregates have not been well defined. We sought to determine the effect of blood collection, handling and processing techniques on detected levels of platelet-monocyte aggregates us...

journal_title：Thrombosis and haemostasis

authors： Harding SA,Din JN,Sarma J,Jessop A,Weatherall M,Fox KA,Newby DE

更新日期：2007-08-01 00:00:00

abstract：:Platelet activation plays a pivotal role in physiological hemostasis and pathological thrombosis causing heart attack and stroke. Previous studies conclude that simultaneous activation of Gi and G12/13 signaling pathways is sufficient to cause platelet aggregation. However, using Gq knockout mice and Gq-specific inhib...

journal_title：Thrombosis and haemostasis

authors： Zhang J,Zhang Y,Zheng S,Liu Y,Chang L,Pan G,Hu L,Zhang S,Liu J,Kim S,Dong J,Ding Z

更新日期：2020-11-01 00:00:00