Low resolution crystal structure of muconolactone isomerase. A decamer with a 5-fold symmetry axis.

abstract：:The light-driven photocycle of rhodopsin begins the photoreceptor cascade that underlies visual response. In a sequence of events, the retinal covalently attached to the rhodopsin protein undergoes a conformational change that communicates local changes to a global conformational change throughout the whole protein. I...

journal_title：Journal of molecular biology

authors： Crozier PS,Stevens MJ,Forrest LR,Woolf TB

更新日期：2003-10-24 00:00:00

abstract：:The most severe form of human malaria is caused by the protozoan parasite Plasmodium falciparum. The primary antigenic and virulence determinant expressed on the surface of infected red blood cells is PfEMP1 (P. falciparum erythrocyte membrane protein 1), a protein that mediates adhesion and sequestration of the paras...

journal_title：Journal of molecular biology

authors： Dzikowski R,Deitsch KW

更新日期：2008-10-03 00:00:00

abstract：:Kinetic models were developed to describe the influence of prolyl peptide bond isomerization on the kinetics of reversible protein folding for cases in which structural intermediates do not occur. In the simulations, the number of prolyl residues and the relative rates of folding and isomerization were varied. The exp...

journal_title：Journal of molecular biology

authors： Kiefhaber T,Kohler HH,Schmid FX

更新日期：1992-03-05 00:00:00

abstract：:Molecular aspects of thermal adaptation of proteins were studied by following the co-evolution of temperature dependence, conformational stability, and substrate specificity in a cold-active lipase modified via directed evolution. We found that the evolution of kinetic stability was accompanied by a relaxation in subs...

journal_title：Journal of molecular biology

authors： Gatti-Lafranconi P,Natalello A,Rehm S,Doglia SM,Pleiss J,Lotti M

更新日期：2010-01-08 00:00:00

abstract：:To study mechanisms of nucleosome positioning, small circular plasmids were constructed, assembled into chromatin in vivo in Saccharomyces cerevisiae, and their chromatin structures were analysed with respect to positions of nucleosomes and nuclease-sensitive regions. Plasmids used include insertions of the URA3 gene ...

journal_title：Journal of molecular biology

authors： Thoma F

更新日期：1986-07-20 00:00:00

abstract：:Alpha-tocopherol transfer protein (alpha-TTP) is a liver protein responsible for the selective retention of alpha-tocopherol from dietary vitamin E, which is a mixture of alpha, beta, gamma, and delta-tocopherols and the corresponding tocotrienols. The alpha-TTP-mediated transfer of alpha-tocopherol into nascent VLDL ...

journal_title：Journal of molecular biology

authors： Meier R,Tomizaki T,Schulze-Briese C,Baumann U,Stocker A

更新日期：2003-08-15 00:00:00

abstract：:alpha-Lactalbumin (alpha-LA) is a two-domain, calcium-binding protein that forms one of the best studied molten globules. We present here amide hydrogen exchange studies of the molten globule formed by human alpha-LA at pH 2 and compare these results with a similar study of the native state at pH 6.3. The most persist...

journal_title：Journal of molecular biology

authors： Schulman BA,Redfield C,Peng ZY,Dobson CM,Kim PS

更新日期：1995-11-10 00:00:00

abstract：:To address the mechanism of thermosensing and its implications for molecular engineering, we previously deconstructed the functional components of the bacterial thermosensor DesK, a histidine kinase with a five-span transmembrane domain that detects temperature changes. The system was first simplified by building a se...

journal_title：Journal of molecular biology

authors： Inda ME,Vazquez DB,Fernández A,Cybulski LE

更新日期：2019-03-01 00:00:00

abstract：:Current clinical anti-CD40 biologic agents include both antagonist molecules for the treatment of autoimmune diseases and agonist molecules for immuno-oncology, yet the relationship between CD40 epitope and these opposing biological outcomes is not well defined. This report describes the identification of potent antag...

journal_title：Journal of molecular biology

authors： Yamniuk AP,Suri A,Krystek SR,Tamura J,Ramamurthy V,Kuhn R,Carroll K,Fleener C,Ryseck R,Cheng L,An Y,Drew P,Grant S,Suchard SJ,Nadler SG,Bryson JW,Sheriff S

更新日期：2016-07-17 00:00:00

abstract：:In Xenopus somatic cells histone H1 effects the transcriptional repression of oocyte type 5 S RNA genes, without altering the transcription of the somatic type 5 S RNA genes. Using an unambiguous nucleosome mapping method we find substantial differences between the multiple in vitro nucleosome positions on the two typ...

journal_title：Journal of molecular biology

authors： Panetta G,Buttinelli M,Flaus A,Richmond TJ,Rhodes D

更新日期：1998-09-25 00:00:00

abstract：:Iron loss in vitro by the iron scavenger bovine lactoferrin was investigated in acidic media in the presence of three different monoanions (NO(3)(-), Cl(-) and Br(-)) and one dianion (SO(4)(2-)). Holo and monoferric C-site lactoferrins lose iron in acidic media (pH< or =3.5) by a four-step mechanism. The first two ste...

journal_title：Journal of molecular biology

authors： Abdallah FB,El Hage Chahine JM

更新日期：2000-10-20 00:00:00

abstract：:The projected structure of the vanadate-induced crystalline aggregates of Ca2+-ATPase molecules in isolated sarcoplasmic reticulum membranes has been determined. The molecules form tubular crystals with an oblique surface lattice having cell dimensions a = 65.9 A, b = 114.4 A and gamma = 77.9 degrees. The space group ...

journal_title：Journal of molecular biology

authors： Taylor K,Dux L,Martonosi A

更新日期：1984-03-25 00:00:00

abstract：:We have studied in reconstitution experiments the conditions under which peptides derived from histones H1 and H5 are bound in chromatin and to what extent they are involved in the organization of chromatin fibers. The fragments of rat liver histone H1 (rH1) and chicken erythrocytes H1 (cH1) and H5 (cH5) used were the...

journal_title：Journal of molecular biology

authors： Thoma F,Losa R,Koller T

更新日期：1983-07-05 00:00:00

abstract：:The soluble domain of the self-assembly vaccinia virus envelope protein A27L, sA27L-aa, consists of a flexible extended coil at the N terminus and a rigid hydrophobic coiled-coil region at the C terminus. In the former, a basic strip of 12 residues is responsible for binding to cell-surface heparan sulfates. Although ...

journal_title：Journal of molecular biology

authors： Ho Y,Hsiao JC,Yang MH,Chung CS,Peng YC,Lin TH,Chang W,Tzou DL

更新日期：2005-06-24 00:00:00

abstract：:The bcl-2 gene encodes a 26kDa protein which functions as a central regulator of apoptosis. Here we investigated the pathway of Bcl-2alpha into the mitochondrial outer membrane using the yeast Saccharomyces cerevisiae as a model organism. We found that interactions of Bcl-2alpha with the mitochondrial import receptor ...

journal_title：Journal of molecular biology

authors： Motz C,Martin H,Krimmer T,Rassow J

更新日期：2002-11-01 00:00:00

abstract：:The algebra of target theory for damage by radiation was laid out by Atwood and Norman in 1949. Their equations provide a widely embraced framework for distinguishing single-hit and multi-hit mechanisms of damage. The present work asks whether in vitro damage to DNA duplexes by different agents affects amplification b...

journal_title：Journal of molecular biology

authors： Spangler R,Goddard NL,Spangler DN,Thaler DS

更新日期：2009-09-18 00:00:00

abstract：:The family of fibroblast growth factor receptors (FGFRs) plays an important and well-characterized role in a variety of pathological disorders. FGFR4 is involved in myogenesis and muscle regeneration. Mutations affecting the kinase domain of FGFR4 may cause cancer, for example, breast cancer or rhabdomyosarcoma. Where...

journal_title：Journal of molecular biology

authors： Lesca E,Lammens A,Huber R,Augustin M

更新日期：2014-11-11 00:00:00

abstract：:Wild-type hen lysozyme has been converted from its soluble native state into highly organized amyloid fibrils. In order to achieve this conversion, conditions were chosen to promote partial unfolding of the native globular fold and included heating of low-pH solutions and addition of organic solvents. Two peptides der...

journal_title：Journal of molecular biology

authors： Krebs MR,Wilkins DK,Chung EW,Pitkeathly MC,Chamberlain AK,Zurdo J,Robinson CV,Dobson CM

更新日期：2000-07-14 00:00:00

abstract：:The non-steroidal anti-estrogen tamoxifen [TAM] has been in clinical use over the last two decades as a potent adjunct chemotherapeutic agent for treatment of breast cancer. It has also been given prophylactically to women with a strong family history of breast cancer. However, tamoxifen treatment has also been associ...

journal_title：Journal of molecular biology

authors： Shimotakahara S,Gorin A,Kolbanovskiy A,Kettani A,Hingerty BE,Amin S,Broyde S,Geacintov N,Patel DJ

更新日期：2000-09-15 00:00:00

abstract：:DNase I digestion of unlabelled chromatin has been used to determine the orientation of nucleosomes in the higher-order structure of chromatin. DNA digestion patterns were analysed quantitatively and compared with simulation curves that were generated with the experimentally obtained rate constants for cutting inside ...

journal_title：Journal of molecular biology

authors： Staynov DZ,Proykova YG

更新日期：1998-05-29 00:00:00

abstract：:To investigate systematically the RNA sequences necessary for aminoacylation by Escherichia coli leucyl-tRNA synthetase, RNAs with leucylation activity were isolated by in vitro selection from a library of tRNALeu variants possessing randomized sequences in the D-loop, the variable arm, and the T-loop. After two round...

journal_title：Journal of molecular biology

authors： Asahara H,Nameki N,Hasegawa T

更新日期：1998-10-30 00:00:00

abstract：:The enzyme mercuric ion reductase MerA is the central component of bacterial mercury resistance encoded by the mer operon. Many MerA proteins possess metallochaperone-like N-terminal domains (NmerA) that can transfer Hg(2+) to the catalytic core domain (Core) for reduction to Hg(0). These domains are tethered to the h...

journal_title：Journal of molecular biology

authors： Johs A,Harwood IM,Parks JM,Nauss RE,Smith JC,Liang L,Miller SM

更新日期：2011-10-28 00:00:00

abstract：:Here, we show that in the chicken genome, the domain of alpha-globin genes is preceded by a CpG island of which the downstream part ( approximately 0.65 kbp) is heavily methylated in lymphoid cells; it is either non-methylated or undermethylated in erythroid cells. Recombinant plasmids were constructed with the corres...

journal_title：Journal of molecular biology

authors： Razin SV,Ioudinkova ES,Scherrer K

更新日期：2000-06-16 00:00:00

abstract：:The expression of immunoglobulin genes is controlled in part by the DNA-binding protein Oct-1 and the B cell-specific transcription co-activator, Bob1 (also known as OCA-B or OBF-1) that together form a complex on the Igkappa promoter. We have characterised the assembly of the ternary complex using biophysical methods...

journal_title：Journal of molecular biology

authors： Chang JF,Phillips K,Lundbäck T,Gstaiger M,Ladbury JE,Luisi B

更新日期：1999-05-21 00:00:00

abstract：:Analysis of the conformational differences between the oxy and deoxy forms of hemoglobin is complicated by shifting coordinate systems and correlated motions between different parts of the molecule. Methods independent of any frame of reference were used to study the differences in structure between the oxy and deoxy ...

journal_title：Journal of molecular biology

authors： Nichols WL,Zimm BH,Ten Eyck LF

更新日期：1997-07-25 00:00:00

abstract：:Escherichia coli nitroreductase is a flavoprotein that reduces a variety of quinone and nitroaromatic substrates. Its ability to convert relatively non-toxic prodrugs such as CB1954 (5-[aziridin-1-yl]-2,4-dinitrobenzamide) into highly cytotoxic derivatives has led to interest in its potential for cancer gene therapy. ...

journal_title：Journal of molecular biology

authors： Lovering AL,Hyde EI,Searle PF,White SA

更新日期：2001-05-25 00:00:00

abstract：:Spectrin repeats fold into triple helical coiled-coils comprising approximately 106 amino acid residues. Using an AFM-related technique we measured the force required to mechanically unfold these repeats to be 25 to 35 pN. Under tension, individual spectrin repeats unfold independently and in an all-or-none process. T...

journal_title：Journal of molecular biology

authors： Rief M,Pascual J,Saraste M,Gaub HE

更新日期：1999-02-19 00:00:00

abstract：:Although numerous measurements of amyloid assembly of different proteins under distinct conditions in vitro have been performed, the molecular mechanisms underlying the specific self-association of proteins into amyloid fibrils remain obscure. Elucidating the nature of the events that initiate amyloid formation remain...

journal_title：Journal of molecular biology

authors： Eichner T,Radford SE

更新日期：2009-03-13 00:00:00

abstract：:IL-22 is a class 2 alpha-helical cytokine involved in the generation of inflammatory responses. These activities require IL-22 to engage the cell surface receptors IL-22R1 and the low-affinity signaling molecule IL-10R2. IL-10R2 also interacts with five other class 2 cytokines: IL-10, IL-26, and the interferon-like cy...

journal_title：Journal of molecular biology

authors： Logsdon NJ,Jones BC,Allman JC,Izotova L,Schwartz B,Pestka S,Walter MR

更新日期：2004-09-10 00:00:00

abstract：:The feline immunodeficiency virus (FIV) is a lentivirus that is related to human immunodeficiency virus (HIV), causing a similar pathology in cats. It is a potential small animal model for AIDS and the FIV-based vectors are also being pursued for human gene therapy. Previous studies have mapped the FIV packaging signa...

journal_title：Journal of molecular biology

authors： Rizvi TA,Kenyon JC,Ali J,Aktar SJ,Phillip PS,Ghazawi A,Mustafa F,Lever AML

更新日期：2010-10-15 00:00:00