Abstract:
:Growth hormone (GH) cells were investigated with the protein A-gold technique on the pituitary glands of tilapia (Sarotherodon mossambicus) and amago salmon (Oncorhynchus rhodurus). By the use of specific antiserum against tilapia GH to both species, the immunoreactive gold particles were demonstrated to be preferentially located on the secretory granules of the GH cells. Specimens fixed only with periodate-lysine-paraformaldehyde (PLP) preserved the hormonal antigenicity well. Osmium postfixation, although considerably reducing the antigenicity and thus resulting in a decrease in number of the gold particles on the GH cells, gave much more satisfactory ultrastructural preservation and immunoreactive localization of immunoreactive material. This investigation demonstrated that, after combined fixation with PLP and PLP-osmium, we could determine the function of a given cell type in various endocrine organs as well as the precise antigenic sites in such cells.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Ueda H,Kagawa H,Fujimoto Sdoi
10.1016/0016-6480(85)90429-0subject
Has Abstractpub_date
1985-07-01 00:00:00pages
149-54issue
1eissn
0016-6480issn
1095-6840pii
0016-6480(85)90429-0journal_volume
59pub_type
杂志文章abstract::We have examined the effect of ambient water temperature on the ability of juvenile rainbow trout to respond to estradiol (E2) injection with vitellogenin (Vg) synthesis. Vg appeared in serum within 24 hr of E2 injection in fish kept at 15 degrees and rose to 70 mg/ml over a 10-day treatment period during which four i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1031
更新日期:1993-02-01 00:00:00
abstract::In the present study we sought to investigate interactions between hypothalamic nitric oxide (NO) and ghrelin signaling on food intake and energy substrate utilization as measured by the respiratory exchange ratio (RER). Guide cannulae were unilaterally implanted in either the arcuate (ArcN) or paraventricular (PVN) n...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.05.017
更新日期:2017-09-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(89)90197-4
更新日期:1989-03-01 00:00:00
abstract::To characterize the involvement of the aromatase gene during the process of sex determination in the European eel (Anguilla anguilla), the expression of its gonadal form was determined during various developmental stages. The cloned cDNA from the European eel gonad (EeCYP19) contains an open reading frame of 1539 bp, ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.06.007
更新日期:2004-09-15 00:00:00
abstract::Previous studies have provided evidence that D-Asp plays a role in steroid-mediated reproductive biology in amphibians, reptiles, birds and mammals. To examine the molecular involvement of D-Asp on steroidogenic pathway regulation, we analysed the expression of StAR, P450 aromatase and 5αRed2 mRNAs in Pelophylax escul...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.10.006
更新日期:2012-01-01 00:00:00
abstract::This study investigated the role of gonadotrophin and steroids in the induction of in vitro oocyte maturation in dab (Limanda limanda) and plaice (Pleuronectes platessa), two marine flatfish (Pleuronectiformes) in which the oocytes mature and ovulate in groups (or batches). Clusters of folliculated oocytes were incuba...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(90)90301-2
更新日期:1990-02-01 00:00:00
abstract::Glucocorticoid hormones allow individuals to rapidly adjust their physiology and behavior to meet the challenges of a variable environment. An individual's baseline concentration of glucocorticoids can reflect shifts in life history stage and resource demands while mediating a suite of physiological and behavioral cha...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.07.006
更新日期:2018-01-15 00:00:00
abstract::Recently, zebrafish has quickly risen as a model species for functional analysis of the brain-pituitary-gonad axis. However, one of the hurdles for such work in this popular model organism is the small size of its pituitary gland, which makes it difficult to investigate the regulation of pituitary hormone expression a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.11.020
更新日期:2009-01-15 00:00:00
abstract::Traditional views suggest that growth hormone and the growth hormone receptor (GH/GHR complex) exert their functions only on the plasma membrane. This paradigm, however, has been challenged by recent new findings that the GH/GHR complex could translocate into cell nuclei where they could still exhibit important physio...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.05.002
更新日期:2018-09-15 00:00:00
abstract::Sublethal doses of potassium thiocyanate (KSCN), an iodide competitor, depressed thyroidal radioiodide uptake of fed laboratory rainbow trout at 11 degrees, but did not depress either plasma levels of T4 (L-thyroxine) and T3 (triiodo-L-thyronine) or radioiodide incorporation into plasma iodothyronines. Furthermore, pl...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90094-1
更新日期:1983-07-01 00:00:00
abstract::We investigated the development of thyroid function during the transition to endothermy in red-winged blackbirds (Agelaius phoeniceus). Thermoregulatory capabilities of blackbirds improve markedly over their relatively short nestling period (10-12 days), with the most striking improvements occurring between days 6 and...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1999.7363
更新日期:1999-11-01 00:00:00
abstract::Oligonucleotide probes were synthesized for the mRNAs of a pair of tilapia prolactins (tPRL177 and tPRL188) and growth hormone (tGH) based on cDNAs for the hormones of Oreochromis niloticus and amino acid sequences for the hormones of O. mossambicus. The three 45mer probes were labeled with 35S for hybridization studi...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1010
更新日期:1993-01-01 00:00:00
abstract::The insulin-like growth factor (IGF) system in teleosts consists of two ligands, IGF-I and IGF-II, multiple binding proteins, and high-affinity transmembrane receptors. There exists a large gap in our knowledge of the structure and expression of receptors mediating the biological effects of the IGFs in teleosts. For e...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1999.7311
更新日期:1999-08-01 00:00:00
abstract::The effects of PGE1, PGE2, PGF2 alpha (2 micrograms/ml), and forskolin (10 microM) on follicle contraction of brook trout (salvelinus fontinalis) were studied by measuring the weight loss of punctured follicles during in vitro incubation. PGE1, PGE2, and forskolin significantly inhibited spontaneous contraction of fol...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90238-f
更新日期:1992-12-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90157-2
更新日期:1987-08-01 00:00:00
abstract::Recent studies have revealed nesfatin-1 as a hypothalamic neuropeptide, regulating food intake, energy expenditure and reproduction primarily by acting on the hypothalamic-pituitary-gonadal axis. Nesfatin-1 is also localized in several peripheral tissues including testes. However, functional significance of nesfatin-1...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.10.022
更新日期:2019-01-15 00:00:00
abstract::We have studied the effects of bovine parathyroid hormone (PTH) and salmon calcitonin (CT) on plasma calcium (Ca) and phosphate levels and on renal function in intact and parathyroidectomized (PTX) chickens, using a simple technique that separates urine from feces. In immature males and egg-laying females, the intrave...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90248-6
更新日期:1987-06-01 00:00:00
abstract::This experiment was performed on two groups of male Japanese quail. One had been maintained in our laboratory as a closed colony (S-group), and the other had been obtained from a commercial source (R-group). Different responses of gonadal function were found between two groups following either testosterone treatment o...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90390-9
更新日期:1985-09-01 00:00:00
abstract::Effects of Al on estradiol-induced vitellogenin (VTG) induction were electrophoretically examined in primary hepatocyte cultures in rainbow trout. Hepatocytes were precultured for 2 days and then estradiol-17 beta (E2, 6 x 10(-6) M) and Al (10(-6)-10(-4) M) were added to the incubation medium. The hepatocytes were cul...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1997.6996
更新日期:1998-01-01 00:00:00
abstract::Early maternal effects in the form of substances accumulated in the egg, such as carotenoids and hormones, can be physiologically relevant for a good development of offspring. It has been found in different species that testosterone (T) can be beneficial to offspring by increasing growth rate, but detrimental by reduc...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2007.12.010
更新日期:2008-04-01 00:00:00
abstract::Three gonadotropin-releasing hormones (GnRHs) and three cognate receptors have been identified in vertebrates, with distinct distributions and functions. According to their sequences, the receptors can be grouped into distinct classes: types I, II, and III. One branch contains all type-I GnRH receptors (GnRH-R-I) from...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2005.01.019
更新日期:2005-05-15 00:00:00
abstract::Understanding reproductive profiles and timing of reproductive events is essential in the management and conservation of humpback whales (Megaptera novaeangliae). Yet compared to other parameters and life history traits, such as abundance, migratory trends, reproductive rates, behavior and communication, relatively li...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2019.03.013
更新日期:2019-08-01 00:00:00
abstract::Isospecific injections of intercerebral commissure (COM) extracts of L. stagnalis, L. palustris, and L. ovata (Lymnaeidae) and of extracts of COM/mediodorsal bodies complexes (COM/DB) of Bulinus truncatus (Bulinidae) and Biomphalaria glabrata (Planorbidae) induced oviposition in a high percentage of the experimental s...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90164-8
更新日期:1983-10-01 00:00:00
abstract::In vitro experiments were conducted to test the hypothesis that 17alpha,20beta-dihydroxy-4-prengnen-3-one (17,20-P) regulates cortisol metabolism in Pacific salmon. In both rainbow trout and coho salmon, cortisol metabolism was significantly higher in the kidney compared to the liver. The rainbow trout kidney converte...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.06.003
更新日期:2010-01-01 00:00:00
abstract::Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2012.11.017
更新日期:2013-01-15 00:00:00
abstract::In in vivo experiments injection of dopamine (DA) or leucine enkephalin (L-Enk) produced hypoglycemia in intact red swamp crayfish, Procambarus clarkii. In contrast, neither compound had a significant effect on the circulating glucose concentration in eyestalkless individuals. With intact crayfish spiperone, a DA rece...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1995.1015
更新日期:1995-01-01 00:00:00
abstract::This article is focused on the effect of specific phase relation of serotonergic and dopaminergic oscillations on the gonadal responses of different avian species. These species include (i) summer breeding birds - Red headed bunting (exhibiting post-reproductive absolute photorefractoriness) and Indian Weaver bird (wh...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.06.019
更新日期:2013-09-01 00:00:00
abstract::By mapping the distribution of cholecystokinin (CCK) receptor types onto an established phylogenetic hypothesis of vertebrate relationships, we tested two hypothesis about the evolution of CCK receptors: (1) A single CCK receptor type, CCK-X, is the ancestral receptor, while CCK-A and CCK-B receptors represent derived...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.0047
更新日期:1996-04-01 00:00:00
abstract::Twelve male edible dormice, captured in the autumn of 1979, were studied for 2 years under natural lighting in an animal enclosure with permanently open windows, at Montpellier, in Southern France (latitude 43 degrees 50' N). Ambient temperature fluctuated annually between +4 and 27 degrees, and the photoperiod betwee...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90266-6
更新日期:1983-06-01 00:00:00
abstract::Yellow catfish (Pelteobagrus fulvidraco) is an important freshwater fish species in China. In particular, an all-male population has been commercially produced for the males grow faster than females. However, the molecular mechanisms underlying sexual dimorphism of body size and sex differentiation are still unclear i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2015.04.015
更新日期:2015-05-15 00:00:00