Crystal structure of ferric Aplysia limacina myoglobin at 2 X 0 A resolution.

abstract：:The antibiotic thiostrepton, a thiazole-containing peptide, inhibits translation and ribosomal GTPase activity by binding directly to a limited and highly conserved region of the large subunit ribosomal RNA termed the GTPase center. We have previously used a filter binding assay to examine the binding of ribosomal pro...

journal_title：Journal of molecular biology

authors： Ryan PC,Lu M,Draper DE

更新日期：1991-10-20 00:00:00

abstract：:Two new human cDNAs, designated phclk2 and phclk3, which have a high identity to the cDNA of the human protein kinase clk, were characterized. Typical features of hclk2 and hclk3 proteins are non-homologous N-terminal regions and the presence of the C-terminal protein kinase domain, which is characteristic for serine/...

journal_title：Journal of molecular biology

authors： Hanes J,von der Kammer H,Klaudiny J,Scheit KH

更新日期：1994-12-16 00:00:00

abstract：:Human serum amyloid P component (SAP) is a normal plasma glycoprotein and the precursor of amyloid P component which is a universal constituent of the abnormal tissue deposits in amyloidosis. X-ray and neutron scattering data showed that pentameric or decameric ring structures for SAP in solution are readily distingui...

journal_title：Journal of molecular biology

authors： Ashton AW,Boehm MK,Gallimore JR,Pepys MB,Perkins SJ

更新日期：1997-09-26 00:00:00

abstract：:Membrane-bound proteases are involved in various regulatory functions. Our previous study indicated that the N-terminal region of an open reading frame, PH1510 (residues 16-236, designated as 1510-N) from the hyperthermophilic archaeon Pyrococcus horikoshii, is a serine protease with a catalytic Ser-Lys dyad that spec...

journal_title：Journal of molecular biology

authors： Yokoyama H,Matsui E,Akiba T,Harata K,Matsui I

更新日期：2006-05-12 00:00:00

abstract：:Phage display, SELEX and other methods of combinatorial chemistry have become very popular means of finding ligands with high affinities to given targets. Despite their success, they suffer from numerous sources of error and bias, such as very low initial concentrations of species, non-specific binding, and the sampli...

journal_title：Journal of molecular biology

authors： Levitan B

更新日期：1998-04-10 00:00:00

abstract：:The periplasmic ribose and glucose-galactose receptors (binding proteins) of Gram-negative bacteria compete for a common inner membrane receptor in bacterial chemotaxis, as well as being the essential primary receptors for their respective membrane transport systems. The high-resolution structures of the periplasmic r...

journal_title：Journal of molecular biology

authors： Mowbray SL

更新日期：1992-09-20 00:00:00

abstract：:The Holliday junction is the central intermediate in homologous recombination. Branch migration of this four-stranded DNA structure is a key step in genetic recombination that affects the extent of genetic information exchanged between two parental DNA molecules. Here, we have constructed synthetic Holliday junctions ...

journal_title：Journal of molecular biology

authors： Prabhu VP,Simons AM,Iwasaki H,Gai D,Simmons DT,Chen J

更新日期：2002-03-08 00:00:00

abstract：:This study describes an algorithm that finds rho-independent transcription terminators in bacterial genomes and evaluates the accuracy of its predictions. The algorithm identifies terminators by searching for a common mRNA motif: a hairpin structure followed by a short uracil-rich region. For each terminator, an energ...

journal_title：Journal of molecular biology

authors： Ermolaeva MD,Khalak HG,White O,Smith HO,Salzberg SL

更新日期：2000-08-04 00:00:00

abstract：:The type IC DNA methyltransferase M.EcoR124I is a trimeric enzyme of 162 kDa consisting of two modification subunits, HsdM, and a single specificity subunit, HsdS. Studies have been largely restricted to the HsdM subunit or to the intact methyltransferase since the HsdS subunit is insoluble when over-expressed indepen...

journal_title：Journal of molecular biology

authors： Smith MA,Read CM,Kneale GG

更新日期：2001-11-16 00:00:00

abstract：:The structure of filamentous bacteriophage Pf1 has been studied using neutron diffraction from magnetically oriented gels of native and valine-deuterated phage. Neutron diffraction intensities were measured to approximately 8 A resolution along the equator and first six layer-lines, and differences due to the deuterat...

journal_title：Journal of molecular biology

authors： Stark W,Glucksman MJ,Makowski L

更新日期：1988-01-05 00:00:00

abstract：:The terminal regions of monomeric hook protein from Salmonella typhimurium are known to be highly mobile and exposed to the solvent. Although hook protein exhibits an unusual far-UV circular dichroism spectrum, resembling that of random coil structures, our calorimetric experiments clearly demonstrate that the molecul...

journal_title：Journal of molecular biology

authors： Vonderviszt F,Závodszky P,Ishimura M,Uedaira H,Namba K

更新日期：1995-08-25 00:00:00

abstract：:Six different hybrid colicins were constructed by recombining various domains of the two pore-forming colicins A and E1. These hybrid colicins were purified and their properties were studied. All of them were active against sensitive cells, although to varying degrees. From the results, one can conclude that: (1) the ...

journal_title：Journal of molecular biology

authors： Benedetti H,Frenette M,Baty D,Knibiehler M,Pattus F,Lazdunski C

更新日期：1991-02-05 00:00:00

abstract：:Efficient gene control by antisense RNA requires rapid bi-molecular interaction with a cognate target RNA. A comparative analysis revealed that a YUNR motif (Y=pyrimidine, R=purine) is ubiquitous in RNA recognition loops in antisense RNA-regulated gene systems. The (Y)UNR sequence motif specifies two intraloop hydroge...

journal_title：Journal of molecular biology

authors： Franch T,Petersen M,Wagner EG,Jacobsen JP,Gerdes K

更新日期：1999-12-17 00:00:00

abstract：:Cytosine deaminase (CD) catalyzes the deamination of cytosine, producing uracil. This enzyme is present in prokaryotes and fungi (but not multicellular eukaryotes) and is an important member of the pyrimidine salvage pathway in those organisms. The same enzyme also catalyzes the conversion of 5-fluorocytosine to 5-flu...

journal_title：Journal of molecular biology

authors： Ireton GC,McDermott G,Black ME,Stoddard BL

更新日期：2002-01-25 00:00:00

abstract：:To elucidate the general constraints imposed on the structure of the D and T-loops in functional tRNAs, active suppressor tRNAs were selected in vivo from a combinatorial tRNA gene library in which several nucleotide positions in these loops were randomized. Analysis of the nucleotide sequences of the selected clones ...

journal_title：Journal of molecular biology

authors： Doyon FR,Zagryadskaya EI,Chen J,Steinberg SV

更新日期：2004-10-08 00:00:00

abstract：:Four subunits of the F1F0-ATPase from bovine heart mitochondria have been produced by heterologous over-expression in Escherichia coli. They are the oligomycin sensitivity conferral protein (OSCP), coupling factor 6 (F6) and subunits b and d. Likewise, fragments b', bI, bC, and bM (amino acid residues 79 to 214, 121 t...

journal_title：Journal of molecular biology

authors： Collinson IR,van Raaij MJ,Runswick MJ,Fearnley IM,Skehel JM,Orriss GL,Miroux B,Walker JE

更新日期：1994-09-30 00:00:00

abstract：:Interleukin (IL)-12 and IL-23 are heterodimeric proinflammatory cytokines that share a common p40 subunit, paired with p35 and p19 subunits, respectively. They represent an attractive class of therapeutic targets for the treatment of psoriasis and other immune-mediated diseases. Ustekinumab is a fully human monoclonal...

journal_title：Journal of molecular biology

authors： Luo J,Wu SJ,Lacy ER,Orlovsky Y,Baker A,Teplyakov A,Obmolova G,Heavner GA,Richter HT,Benson J

更新日期：2010-10-08 00:00:00

abstract：:The bacteriophage P1 recombinase Cre mediates site-specific recombination between loxP sites. The loxP site consists of two 13 base-pair inverted repeats separated by an eight base-pair spacer region. When DNA containing the loxP site is incubated with Cre, specific cleavages occur within the spacer region, creating a...

journal_title：Journal of molecular biology

authors： Hoess RH,Abremski K

更新日期：1985-02-05 00:00:00

abstract：:Bovine enterovirus (BEV) strain VG-5-27 derived from an infectious cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2.7 A. The crystal system is monoclinic,...

journal_title：Journal of molecular biology

authors： Smyth M,Fry E,Stuart D,Lyons C,Hoey E,Martin SJ

更新日期：1993-06-05 00:00:00

abstract：:The backbone dynamics of the cytokine interleukin-8, a symmetric homodimer of overall molecular mass 16 kDa, has been investigated at pH 5.2 by means of 15N relaxation measurements using heteronuclear two-dimensional inverse detected 1H-15N spectroscopy. 15N T1, T2 and NOE data were obtained for 66 out of a total of 6...

journal_title：Journal of molecular biology

authors： Grasberger BL,Gronenborn AM,Clore GM

更新日期：1993-03-20 00:00:00

abstract：:Spider dragline silk, one of the strongest polymers in nature, is composed of proteins termed major ampullate spidroin (MaSp) 1 and MaSp2. The N-terminal (NT) domain of MaSp1 produced by the nursery web spider Euprosthenops australis acts as a pH-sensitive relay, mediating spidroin assembly at around pH 6.3. Using ami...

journal_title：Journal of molecular biology

authors： Landreh M,Askarieh G,Nordling K,Hedhammar M,Rising A,Casals C,Astorga-Wells J,Alvelius G,Knight SD,Johansson J,Jörnvall H,Bergman T

更新日期：2010-11-26 00:00:00

abstract：:Most bacteria possess two type IIA topoisomerases, DNA gyrase and topo IV, that together help manage chromosome integrity and topology. Gyrase primarily introduces negative supercoils into DNA, an activity mediated by the C-terminal domain of its DNA binding subunit (GyrA). Although closely related to gyrase, topo IV ...

journal_title：Journal of molecular biology

authors： Corbett KD,Schoeffler AJ,Thomsen ND,Berger JM

更新日期：2005-08-19 00:00:00

abstract：:Voltage-dependent H(+) (Hv) channels mediate proton conduction into and out of cells under the control of membrane voltage. Hv channels are unusual compared to voltage-dependent K(+), Na(+), and Ca(2+) channels in that Hv channel genes encode a voltage sensor domain (VSD) without a pore domain. The H(+) currents obser...

journal_title：Journal of molecular biology

authors： Lee SY,Letts JA,MacKinnon R

更新日期：2009-04-17 00:00:00

abstract：:TWINKLE is the helicase at the mitochondrial DNA (mtDNA) replication fork in mammalian cells. Mutations in the PEO1 gene, which encodes TWINKLE, cause autosomal dominant progressive external ophthalmoplegia (AdPEO), a disorder associated with deletions in mtDNA. Here, we characterized seven different AdPEO-causing mut...

journal_title：Journal of molecular biology

authors： Korhonen JA,Pande V,Holmlund T,Farge G,Pham XH,Nilsson L,Falkenberg M

更新日期：2008-03-28 00:00:00

abstract：:Nitroaromatic compounds are used extensively in many industrial processes and have been released into the environment where they are considered environmental pollutants. Nitroaromatic compounds, in general, are resistant to oxidative attack due to the electron-withdrawing nature of the nitro groups and the stability o...

journal_title：Journal of molecular biology

authors： Friemann R,Ivkovic-Jensen MM,Lessner DJ,Yu CL,Gibson DT,Parales RE,Eklund H,Ramaswamy S

更新日期：2005-05-20 00:00:00

abstract：:The multimeric membrane protein complex translocase mediates the transport of preproteins across and integration of membrane proteins into the inner membrane of Escherichia coli. The translocase consists of the peripheral membrane-associated ATPase SecA and the heterotrimeric channel-forming complex consisting of SecY...

journal_title：Journal of molecular biology

authors： Scheuring J,Braun N,Nothdurft L,Stumpf M,Veenendaal AK,Kol S,van der Does C,Driessen AJ,Weinkauf S

更新日期：2005-11-25 00:00:00

abstract：:Human prostate-specific antigen (PSA or KLK3) is an important marker for the diagnosis and management of prostate cancer. This is an androgen-regulated glycoprotein of the kallikrein-related protease family secreted by prostatic epithelial cells. Its physiological function is to cleave semenogelins in the seminal coag...

journal_title：Journal of molecular biology

authors： Ménez R,Michel S,Muller BH,Bossus M,Ducancel F,Jolivet-Reynaud C,Stura EA

更新日期：2008-02-29 00:00:00

abstract：:Initiation of transcription at malEp and malKp, two divergent Escherichia coli promoters, depends on the presence of both CRP, a pleiotropic activator, and MalT, the maltose regulon activator. We carried out in vivo genetic and functional analysis of these promoters and characterized their interaction with MalT and CR...

journal_title：Journal of molecular biology

authors： Raibaud O,Vidal-Ingigliardi D,Richet E

更新日期：1989-02-05 00:00:00

abstract：:We have constructed complexes of ribosomal proteins S8, S15, S8 + S15 and S8 + S15 + S6 + S18 with 16 S ribosomal RNA, and probed the RNA moiety with a set of structure-specific chemical and enzymatic probes. Our results show the following effects of assembly of proteins on the reactivity of specific nucleotides in 16...

journal_title：Journal of molecular biology

authors： Svensson P,Changchien LM,Craven GR,Noller HF

更新日期：1988-03-20 00:00:00

abstract：:The MA (matrix) domain of the retroviral Gag polyprotein plays several critical roles during virus assembly. Although best known for targeting the Gag polyprotein to the inner leaflet of the plasma membrane for virus budding, recent studies have revealed that MA also contributes to selective packaging of the genomic R...

journal_title：Journal of molecular biology

authors： Parent LJ,Gudleski N

更新日期：2011-07-22 00:00:00