Abstract:
:Nitroaromatic compounds are used extensively in many industrial processes and have been released into the environment where they are considered environmental pollutants. Nitroaromatic compounds, in general, are resistant to oxidative attack due to the electron-withdrawing nature of the nitro groups and the stability of the benzene ring. However, the bacterium Comamonas sp. strain JS765 can grow with nitrobenzene as a sole source of carbon, nitrogen and energy. Biodegradation is initiated by the nitrobenzene dioxygenase (NBDO) system. We have determined the structure of NBDO, which has a hetero-hexameric structure similar to that of several other Rieske non-heme iron dioxygenases. The catalytic subunit contains a Rieske iron-sulfur center and an active-site mononuclear iron atom. The structures of complexes with substrates nitrobenzene and 3-nitrotoluene reveal the structural basis for its activity with nitroarenes. The substrate pocket contains an asparagine residue that forms a hydrogen bond to the nitro-group of the substrate, and orients the substrate in relation to the active-site mononuclear iron atom, positioning the molecule for oxidation at the nitro-substituted carbon.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Friemann R,Ivkovic-Jensen MM,Lessner DJ,Yu CL,Gibson DT,Parales RE,Eklund H,Ramaswamy Sdoi
10.1016/j.jmb.2005.03.052keywords:
subject
Has Abstractpub_date
2005-05-20 00:00:00pages
1139-51issue
5eissn
0022-2836issn
1089-8638pii
S0022-2836(05)00332-3journal_volume
348pub_type
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