Abstract:
:7-Hydroxymethylbenz[a]anthracene (7-HBA) showed potent mutagenicity towards Salmonella typhimurium TA 98 in the presence of untreated rat liver cytosol fortified with the PAPS-generating system, sodium sulphate and ATP. No mutagenic activity was observed either when the cytosol was boiled or when sodium sulphate or ATP was omitted from the assay medium. A highly reactive sulphate ester of 7-HBA was isolated and identified from the medium. It had a half-life of 3.5 min at 37 degrees C and pH 7.4 in water and showed potent, intrinsic mutagenicity towards TA 98. The mutagenicity of 7-HBA sulphate was almost completely retarded in the presence of the cytosol and glutathione. From the biological systems containing glutathione a non-mutagenic and stable glutathione conjugate was isolated that was assigned as S-(benz[a]anthracen-7-yl)methylglutathione. 7-HBA sulphate covalently bound to calf thymus DNA and cytosolic proteins. A fluorospectroscopic study indicated that the carcinogen bound to the biomacromolecules through its 7-methylene group with loss of a sulphate anion as a leaving group.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Watabe T,Hakamata Y,Hiratsuka A,Ogura Kdoi
10.1093/carcin/7.2.207subject
Has Abstractpub_date
1986-02-01 00:00:00pages
207-14issue
2eissn
0143-3334issn
1460-2180journal_volume
7pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::The DNA damaging and mutagenic activities of procarbazine, a methylating drug employed in cancer chemotherapy and suspected of causing therapy-related leukaemia, were investigated in the liver and bone marrow of lambda lacZ transgenic mice (MutaMouse). The drug was administered using two different protocols, a 'high-d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.11.2191
更新日期:1997-11-01 00:00:00
abstract::To investigate the mechanistic basis for the biological properties of anthocyanins, two aglycone anthocyanins [delphinidin (DY) and cyanidin (CY)] were used to examine their effects on cell cycle progression and on induction of apoptosis in human cancer cells (uterine carcinoma and colon adenocarcinoma cells) and in n...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh138
更新日期:2004-08-01 00:00:00
abstract::There is increasing concern that compounds formed during the chemical or biological transformation of pollutants in the environment may be more detrimental to human and environmental health than the original pollutant. In this study, two bacterial transformation products of polycyclic aromatic hydrocarbons (PAHs), pyr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh174
更新日期:2004-09-01 00:00:00
abstract::Skin is a major target of carcinogenic trivalent arsenic (arsenite, As3+). It has been thought that cell proliferation is one of the central events involved in the carcinogenic effect of arsenite. Cyclin D1, a nuclear protein playing a pivotal role in cell proliferation and cell cycle transition from G1 to S phases, h...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi321
更新日期:2006-04-01 00:00:00
abstract::Evidence from live cell bioassays shows that the flat mucosa from patients with colon cancer exhibits resistance to bile salt-induced apoptosis. Three independent cell lines derived from the colonic epithelial cell line HCT-116 were selected for resistance to bile salt-induced apoptosis. These cell lines were develope...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.12.2063
更新日期:2002-12-01 00:00:00
abstract::Many chemoradiation therapies cause DNA damage through oxidative stress. An important cellular mechanism that protects cells against oxidative stress involves DNA repair. One of the primary DNA repair mechanisms for oxidative DNA damage is base excision repair (BER). BER involves the tightly coordinated function of fo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm072
更新日期:2007-08-01 00:00:00
abstract::3-tert-Butyl-4-hydroxyanisole (3-BHA) and its metabolites, tert-butyl-1,4-benzoquinone (BQ) and 3-tert-butyl-5-methoxy-1,2-benzoquinone (BHAoQ), were tested for initiating activity in a two-stage transformation assay using BALB/3T3 cells. Cells were treated first with a test chemical and then with the tumor promoter 1...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.11.1985
更新日期:1990-11-01 00:00:00
abstract::The liver-derived human cell line, Hep G2, has high benzo[a]pyrene-metabolizing activity and converts benzo[a]pyrene to intermediates that are mutagenic and that bind to DNA. This cell line will be useful for studying metabolic activation of polycyclic aromatic hydrocarbons and other xenobiotics by human tissue and as...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.10.871
更新日期:1980-01-01 00:00:00
abstract::Comparative in vitro metabolic experiments with 7,8-benzoflavone using microsomes prepared from the livers of control Sprague-Dawley rats or from rats of the same strain treated with phenobarbitone, 3-methylcholanthrene, or Aroclor 1254 (known inducers of hepatic cytochrome P450 mixed function oxidases) disclose varia...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.2.229
更新日期:1982-01-01 00:00:00
abstract::Oral squamous cell carcinoma (SCC) is among the most prevalent cancers in the world and is characterized by high morbidity and few therapeutic options. Like most cancers, oral SCC arises from a multistep process involving alterations of genes responsible for balancing proliferation and differentiation. Among these, Kr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt349
更新日期:2014-03-01 00:00:00
abstract::Eukaryotic 3'-end formation is a critical step for mRNA maturation and involves a requisite cleavage and polyadenylation event downstream of a polyadenylation signal. Recent discoveries suggest that in nearly 70% of human genes, cleavage and polyadenylation can occur at multiple locations through a process known as al...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgx096
更新日期:2018-01-12 00:00:00
abstract::Colorectal cancer (CRC) is the fourth leading cause of cancer mortality worldwide. Genome-wide association studies (GWAS) identified more than 50 CRC loci. However, most of the previous studies were conducted in European population, and host genetic factors among Japanese population are largely remained to be identifi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy026
更新日期:2018-05-03 00:00:00
abstract::The tissue distribution and macromolecular binding of benzene was studied over a dose range spanning nine-orders of magnitude to determine the nature of the dose-response and to establish benzene's internal dosimetry at doses encompassing human environmental exposures. [14C]-Benzene was administered to B6C3F1 male mic...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.12.2421
更新日期:1997-12-01 00:00:00
abstract::We examined allelic loss in colon tumors induced by 1,2-dimethylhydrazine (DMH) in F1 hybrid mice, using sequence-tagged microsatellite site (STMS) primers derived from the chromosomal region closely linked to the p53 locus. Polymerase chain reaction--single-strand conformation polymorphism (PCR-SSCP) analysis of 155 ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.7.1483
更新日期:1993-07-01 00:00:00
abstract::The rat liver 9000 g supernatant mediated metabolism of the carcinogenic aristolochic acid, which consists of aristolochic acid I (AAI) and aristolochic acid II (AAII), was investigated. Under anaerobic conditions the major metabolites were the corresponding aristolactams for both AAI and AAII. In contrast under aerob...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.1.59
更新日期:1986-01-01 00:00:00
abstract::The metabolism in mouse epidermis of benzo[b]fluoranthene (BbF) was studied. [3H]BbF was applied topically, mice were killed at various intervals, and metabolites were extracted from the epidermis and analyzed by h.p.l.c. The major metabolites were identified by comparisons to standards as 4-, 5-, and 6-hydroxyBbF. Su...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.11.1579
更新日期:1987-11-01 00:00:00
abstract::Childhood cancer is the leading cause of disease-related death in children aged 1-14 years in Canada and the USA and it has been hypothesized that transplacental exposure to environmental carcinogens such as benzene may contribute to the etiology of these cancers. Our objectives were to determine if transplacental ben...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq074
更新日期:2010-06-01 00:00:00
abstract::Although the alternative splicing of various genes is a common event in human tumors, the mechanisms behind it have not been characterized. We hypothesized that the expression of splicing regulatory factors would be changed during cellular transformation. Gene expression of three splicing regulatory factors, alternati...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.12.2341
更新日期:1999-12-01 00:00:00
abstract::Glucosinolates are common components of cruciferous vegetables that can be hydrolyzed during food processing to yield isothiocyanates, some of which have been shown to inhibit the induction of mammary tumors in rats by 7,12-dimethyl-benz[a]anthracene. To determine how intact glucosinolates affect the metabolism of ben...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.4.657
更新日期:1988-04-01 00:00:00
abstract::The effect of whole body ionising radiation from a linear accelerator on rat tissue O6-methylguanine (O6-meG) methyltransferase (MT) activity has been assessed using an assay which measures the transfer of 3H-radioactivity from 3H-methylated substrate DNA to protein. The maximal effect occurred 2 days after a 1 krad d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.12.1699
更新日期:1985-12-01 00:00:00
abstract::Base excision repair pathway may play an important role in repairing DNA damage related to Helicobacter pylori-induced inflammatory process. To evaluate the association between genetic polymorphisms of X-ray repair cross-complementing group 1 (XRCC1, Arg194Trp and Arg399Gln), adenosine diphosphate ribosyl transferase ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp018
更新日期:2009-03-01 00:00:00
abstract::Apurinic/apyrimidinic endonuclease/redox effector factor-1 (APE/Ref-1) is a multifunctional protein involved both in DNA base excision repair and redox regulation. Studies have suggested that abnormal Ref-1 levels and/or activities are associated with tumor progression and sensitivities to treatment, but no direct evi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm128
更新日期:2007-11-01 00:00:00
abstract::We present data showing that the major phenobarbital inducible cytochromes P-450 (cytochrome P-450IIB1 and cytochrome P-450IIB2) were phosphorylated in intact hepatocytes. This phosphorylation was greatly increased by the cAMP derivatives N6-dibutyryl-cAMP and 8-thiomethyl-cAMP mediated by a cAMP-dependent protein kin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.1.225
更新日期:1989-01-01 00:00:00
abstract::To evaluate the significance of C-C chemokine receptor type 5 (CCR5) in lung tumor development, we compared carcinogen-induced tumor growth in CCR5 knockout (CCR5(-/-)) mice and wild-type (CCR5(+/+)) mice. CCR5(-/-) mice showed reduced urethane (1g/kg)-induced tumor incidence when compared with those of CCR5(+/+) mice...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs265
更新日期:2012-12-01 00:00:00
abstract::Several members of the P450 family, including cytochrome P450 1B1 (CYP1B1), can convert tobacco smoke (TS) procarcinogens, including benzo[a]pyrene (B[a]P), to carcinogenic intermediates. In this study we investigated the effects of TS condensate and B[a]P on the expression of CYP1B1 in vitro and in vivo. CYP1B1 mRNA ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh243
更新日期:2004-11-01 00:00:00
abstract::SENCAR mice are unusually sensitive to induction of papillomas and squamous cell carcinomas by initiation with 7,12-dimethylbenzanthracene (DMBA) and promotion by 12-O-tetradecanoylphorbol-13-acetate (TPA). Tumors induced by this protocol were tested for the presence of papillomavirus by immunohistochemistry, Southern...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.2.341
更新日期:1990-02-01 00:00:00
abstract::The organochlorine pesticides chlordane and heptachlor were demonstrated to be liver neoplasm promoters in mice. Male B6C3F1 mice exposed to diethylnitrosamine (DEN) for 14 weeks followed by 25 weeks on control diet developed a 40% incidence of liver neoplasms whereas those given chlordane or heptachlor afterwards had...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.12.1689
更新日期:1984-12-01 00:00:00
abstract::Receptor tyrosine kinase EphB3 is expressed in cells in the bottom of intestinal crypts near stem cell niches. Loss of Ephb3 has recently been reported to produce invasive colorectal carcinoma in Apc(Min/+) mice and EphB-mediated compartmentalization was demonstrated to be a mechanism suppressing colorectal cancer pro...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp133
更新日期:2009-09-01 00:00:00
abstract::Treatment of SV40-infected CV-1 cells with the ultimate carcinogen anti-benzo[a]pyrene diol epoxide (BPDE) at 1 X 10(-4) mg/ml or higher reduced the rate of viral DNA synthesis to an extent dependent on the BPDE concentration; similar reductions in cellular DNA synthesis were produced in infected and uninfected cells....
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.2.125
更新日期:1983-01-01 00:00:00
abstract::The carcinogenicity of two commonly used brown and black varieties of masheri, a pyrolysed tobacco product, was studied by feeding the masheri through the diet at a 10% level to three different animal species of both sexes. In Sprague-Dawley rats, only brown masheri was used, while in Swiss mice and Syrian golden hams...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.11.2137
更新日期:1988-11-01 00:00:00