当前位置： SCI文献检索 > JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH期刊下所有文献 > Flow cytometric analysis of CK19 expression in the peripheral blood of breast carcinoma patients: relevance for circulating tumor cell detection.

Flow cytometric analysis of CK19 expression in the peripheral blood of breast carcinoma patients: relevance for circulating tumor cell detection.

Abstract：

BACKGROUND:Immunocytochemistry and RT-PCR have been widely used for the detection of circulating tumor cells in patients with breast cancer but their specificity is limited. Our purpose is to utilize a convenient and specific technology to detect circulating tumor cells in breast cancer patients. METHODS:To determine the sensitivity and specificity of our method, A431 cells were serially diluted with human peripheral blood leukocytes and stained with CK19. A total of 73 blood specimens including 25 healthy volunteers and 48 patients with breast carcinoma and benign tumor were tested by flow cytometry to quantify the expression of CK19. RESULTS:The detectable upper limit of A431 cells was 1 cancer cell among 104 human white blood cells. CK19 was detected in 27% of breast cancer patients but none control gives positive result. The number of cancer cells increased gradually along with the disease stages for it was the least in stage I (0%) and the most in stage IV (1.29%). Fifteen patients were observed during three month chemotherapy after surgery, and most of their CK19 expression levels declined after treatment. CONCLUSION:Our research convinces that the detection of CK19 in peripheral blood by flow cytometry is also a specific and feasible method to monitor circulating tumor cells in breast cancer.

journal_name

J Exp Clin Cancer Res

journal_title

Journal of experimental & clinical cancer research : CR

authors

Wang L,Wang Y,Liu Y,Cheng M,Wu X,Wei H

doi

10.1186/1756-9966-28-57

subject

Has Abstract

pub_date

2009-04-28 00:00:00

pages

57

eissn

0392-9078

issn

1756-9966

pii

1756-9966-28-57

journal_volume

28

pub_type

杂志文章

相关文献

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH文献大全
  • Glucose-6-phosphate dehydrogenase blockade potentiates tyrosine kinase inhibitor effect on breast cancer cells through autophagy perturbation.

    abstract：BACKGROUND:Glucose-6-phospate dehydrogenase (G6PD) is the limiting enzyme of the pentose phosphate pathway (PPP) correlated to cancer progression and drug resistance. We previously showed that G6PD inhibition leads to Endoplasmic Reticulum (ER) stress often associated to autophagy deregulation. The latter can be induce...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1164-5

    authors： Mele L,la Noce M,Paino F,Regad T,Wagner S,Liccardo D,Papaccio G,Lombardi A,Caraglia M,Tirino V,Desiderio V,Papaccio F

    更新日期：2019-04-12 00:00:00

  • Cdk1/P34Cdc2 and P21waf expression in colorectal adenomas and carcinomas.

    abstract：:p21WAF1,CIP1,SDI 1 is a specific inhibitor of cyclin-dependent kinase and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Cdk1/p34cdc2 plays a crucial role during mitosis. The immunoreactivity of p21 WAF1,CIP1,SDI 1 and cdk1/p34cdc2 was evaluated in adenomas and adenocarcinomas of the hum...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Egilmez R,Elagöz S,Kanik EA

    更新日期：2001-12-01 00:00:00

  • A pseudogene-signature in glioma predicts survival.

    abstract：:Pseudogene was recognized as a potential tumor suppressor or oncogene in varies of diseases, however its roles in glioma have not been investigated. Our study was to identify the pseudogene-signature that predicted glioma survival. Using a pseudogene-mining approach, we performed pseudogene expression profiling in 183...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-015-0137-6

    authors： Gao KM,Chen XC,Zhang JX,Wang Y,Yan W,You YP

    更新日期：2015-03-04 00:00:00

  • Transfection of mammalian cells by the methods of receptor mediated gene transfer and particle bombardment.

    abstract：:The efficacy of currently developed methods for gene transfer into mammalian cells depends primarily on the transfection technique, and also on the type of targeted cells. Considering the importance of gene transfer in the creation of gene therapies, our study was aimed at the assessment of transfection capacity of re...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Novaković S,Knezević M,Golouh R,Jezersek B

    更新日期：1999-12-01 00:00:00

  • Intrinsic fluorescence of the clinically approved multikinase inhibitor nintedanib reveals lysosomal sequestration as resistance mechanism in FGFR-driven lung cancer.

    abstract：BACKGROUND:Studying the intracellular distribution of pharmacological agents, including anticancer compounds, is of central importance in biomedical research. It constitutes a prerequisite for a better understanding of the molecular mechanisms underlying drug action and resistance development. Hyperactivated fibroblast...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0592-3

    authors： Englinger B,Kallus S,Senkiv J,Heilos D,Gabler L,van Schoonhoven S,Terenzi A,Moser P,Pirker C,Timelthaler G,Jäger W,Kowol CR,Heffeter P,Grusch M,Berger W

    更新日期：2017-09-07 00:00:00

  • Malignant glucagonoma. New options of treatment.

    abstract：:Few cases of malignant glucagonomas have been described in the literature. In this paper we present a case of a 77-year-old woman with necrolytic migratory erythema and high plasma glucagon and chromogranin A levels caused by a neuroendocrine tumour. An abdominal CT scan suggested a pancreatic lesion and two liver met...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Appetecchia M,Ferretti E,Carducci M,Izzo F,Carpanese L,Marandino F,Terzoli E

    更新日期：2006-03-01 00:00:00

  • Over-expression of long noncoding RNA BANCR inhibits malignant phenotypes of human bladder cancer.

    abstract：BACKGROUND:Accumulating evidences indicated that lncRNAs play crucial regulatory roles in oncogenesis and progression of cancers. BRAF activated non-coding RNA (BANCR) has been identified to contribute to the progression of some human cancers. However, the relationship between BANCR and bladder cancer (BC) is largely u...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0397-9

    authors： He A,Liu Y,Chen Z,Li J,Chen M,Liu L,Liao X,Lv Z,Zhan Y,Zhuang C,Lin J,Huang W,Mei H

    更新日期：2016-08-11 00:00:00

  • Neuroendocrine differentiation in follicle-cell thyroid carcinoma: correlation to prognostic factors in papillary carcinoma.

    abstract：:The presence of NE-differentiation in the follicle-cell thyroid carcinoma has been investigated by immunohistochemical detection of chromogranin A in the neoplastic cells. It has been found that NE-differentiation was present in a significant percentage in the papillary Ca alone (46.60%) whereas all Huerthle-cell neop...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Tseleni-Balafouta S,Kavantzas N,Alevizaki M,Paraskevakou H,Davaris P

    更新日期：1998-12-01 00:00:00

  • Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma.

    abstract：BACKGROUND:Despite the improvement of relapse-free survival mediated by anti-angiogenic drugs like sunitinib (Sutent®), or by combinations of anti-angiogenic drugs with immunotherapy, metastatic clear cell Renal Cell Carcinoma (mccRCC) remain incurable. Hence, new relevant treatments are urgently needed. The VEGFs core...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-021-01832-x

    authors： Dumond A,Brachet E,Durivault J,Vial V,Puszko AK,Lepelletier Y,Montemagno C,Pagnuzzi-Boncompagni M,Hermine O,Garbay C,Lagarde N,Montes M,Demange L,Grépin R,Pagès G

    更新日期：2021-01-18 00:00:00

  • Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression.

    abstract：BACKGROUND:Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. MATERIALS AND METHODS:qRT-PCR and western blot were conducted to measure relev...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01551-9

    authors： Rong Z,Wang Z,Wang X,Qin C,Geng W

    更新日期：2020-04-09 00:00:00

  • Long non-coding RNA small nucleolar RNA host gene 12 (SNHG12) promotes tumorigenesis and metastasis by targeting miR-199a/b-5p in hepatocellular carcinoma.

    abstract：BACKGROUND:Hepatocellular carcinoma (HCC) is third leading cause of cancer-related death globally. Evidence suggest that long non-coding RNAs (lncRNAs) have emerged as key regulators of tumorigenesis and metastasis in HCC. In this study, we investigated the functional significance of SNHG12 and explored whether SNHG12 ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0486-9

    authors： Lan T,Ma W,Hong Z,Wu L,Chen X,Yuan Y

    更新日期：2017-01-10 00:00:00

  • Synergistic anti-tumor effect of ultrasound and hematoporphyrin on sarcoma180 cells with special reference to the changes of morphology and cytochrome oxidase activity of tumor cells.

    abstract：:This study is aimed at evaluating the inhibitory effects of the association of hematoporphyrin and ultrasound at variable intensities with a fixed frequency of 1.1MHz in tumor nodules. Specifically, the effects were studied both in solid and ascitic S180 tumors transplanted in mice by clinical, cytochemical and ultras...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Quan-hong L,Shi-hui S,Ya-ping X,Hao Q,Jin-xuan Z,Yao-hui R,Meng L,Pan W

    更新日期：2004-06-01 00:00:00

  • Linc-ROR promotes esophageal squamous cell carcinoma progression through the derepression of SOX9.

    abstract：BACKGROUND:Novel therapies tailored to the molecular composition of esophageal squamous cell carcinoma (ESCC) are needed to improve patient survival. We investigated the regulatory network of long intergenic non-protein coding RNA, regulator of reprogramming (linc-ROR) and sex-determining region Y-box 9 (SOX9), and the...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0658-2

    authors： Wang L,Yu X,Zhang Z,Pang L,Xu J,Jiang J,Liang W,Chai Y,Hou J,Li F

    更新日期：2017-12-13 00:00:00

  • Up-regulation of VANGL1 by IGF2BPs and miR-29b-3p attenuates the detrimental effect of irradiation on lung adenocarcinoma.

    abstract：:Accumulating evidence suggests that radiation treatment causes an adaptive response of lung adenocarcinoma (LUAD), which in turn attenuates the lethal effect of the irradiation. Previous microarray assays manifested the change of gene expression profile after irradiation. Bioinformatics analysis of the significantly c...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-020-01772-y

    authors： Hao CC,Xu CY,Zhao XY,Luo JN,Wang G,Zhao LH,Ge X,Ge XF

    更新日期：2020-11-23 00:00:00

  • HOXD9 promotes the growth, invasion and metastasis of gastric cancer cells by transcriptional activation of RUFY3.

    abstract：BACKGROUND:The transcription factor HOXD9 is one of the members of the HOX family, which plays an important role in neoplastic processes. However, the role of HOXD9 in the growth and metastasis of gastric cancer (GC) remains to be elucidated. METHODS:In vitro functional role of HOXD9 and RURY3 in GC cells was determin...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1399-1

    authors： Zhu H,Dai W,Li J,Xiang L,Wu X,Tang W,Chen Y,Yang Q,Liu M,Xiao Y,Zhang W,Lin J,Wang J,Liu G,Sun Y,Jiang P,Li G,Li A,Liu S,Chen Y,Wang J

    更新日期：2019-09-23 00:00:00

  • NF-KappaB expression correlates with apoptosis and angiogenesis in clear cell renal cell carcinoma tissues.

    abstract：BACKGROUND:Clear cell renal cell carcinoma (ccRCC) is the most frequently encountered tumor in the adult kidney. Many factors are known to take part in the development and progression of this tumor. Nuclear factor kappa B (NF-kappaB) is a family of the genes that includes five members acting in events such as inflammat...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-27-53

    authors： Meteoglu I,Erdogdu IH,Meydan N,Erkus M,Barutca S

    更新日期：2008-10-19 00:00:00

  • LSH interacts with and stabilizes GINS4 transcript that promotes tumourigenesis in non-small cell lung cancer.

    abstract：BACKGROUND:Elucidating mechanisms in oncogenes and epigenetic modifiers are needed to gain insights into the etiology and treatment of cancer, regulation of oncogene by chromatin modifiers at post-transcriptional level is critical and remains unclear. We have investigated the role of GINS4 in NSCLC. METHODS:The expres...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1276-y

    authors： Yang R,Liu N,Chen L,Jiang Y,Shi Y,Mao C,Liu Y,Wang M,Lai W,Tang H,Gao M,Xiao D,Wang X,Yu F,Cao Y,Yan Q,Liu S,Tao Y

    更新日期：2019-06-28 00:00:00

  • In vivo molecular targeting effects of anti-Sp17- ICG-Der-02 on hepatocellular carcinoma evaluated by an optical imaging system.

    abstract：BACKGROUND:As the expression of human sperm protein 17 (Sp17) in normal tissue is limited and the function is obscure, its aberrant expression in malignant tumors makes it to be a candidated molecular marker for tumor imaging diagnosis and targeting therapy of the diseases.The aim of this research is to evaluate the ta...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/1756-9966-30-25

    authors： Li FQ,Zhang SX,An LX,Gu YQ

    更新日期：2011-03-03 00:00:00

  • Breast carcinomas with low amplified/equivocal HER2 by Ish: potential supporting role of multiplex ligation-dependent probe amplification.

    abstract：BACKGROUND:This is a retrospective cross sectional study aimed to verify whether Multiplex Ligation-dependent Probe Amplification (MLPA), a quantitative molecular assay, may represent a valuable reflex test in breast cancer with equivocal HER2 expression by immunohistochemistry and HER2 gene signals/nucleus (s/n) rangi...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0613-2

    authors： Ercolani C,Marchiò C,Di Benedetto A,Fabi A,Perracchio L,Vici P,Sperati F,Buglioni S,Arena V,Pescarmona E,Sapino A,Terrenato I,Mottolese M

    更新日期：2017-10-13 00:00:00

  • The immunoregulatory mechanisms of carcinoma for its survival and development.

    abstract：:The immune system in patients detects and eliminates tumor cells, but tumors still progress persistently. The mechanisms by which tumor cells survive under the pressure of immune surveillance are not fully understood. This review is to present the evidence from clinical studies, showing a significant correlation of cl...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章,评审

    doi：10.1186/1756-9966-30-12

    authors： Du C,Wang Y

    更新日期：2011-01-21 00:00:00

  • High glucose dephosphorylates serine 46 and inhibits p53 apoptotic activity.

    abstract：BACKGROUND:In response to diverse genotoxic stimuli p53 is activated as transcription factor to exert its tumor-suppressor function. P53 activation requires protein stabilization, nuclear localization and posttranslational modifications in key residues that may influence p53 selection of target genes. Among them, serin...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-014-0079-4

    authors： Garufi A,D'Orazi G

    更新日期：2014-09-27 00:00:00

  • Cost of de novo acute myeloid leukemia induction therapy in adults: analysis of EORTC-GIMEMA AML10 and FLANG regimens.

    abstract：:Since the social and financial impact of AML therapy is becoming more and more relevant we analyzed the cost of induction therapy of two different regimens. The first one is part of the widely employed EORTC-GIMEMA AML-10 and consists often days of therapy. The second (FLANG) is a short (three day), Fludarabine, Ara-C...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Clavio M,Quintino S,Masoudi B,Carrara S,Cerri R,Pierri I,Canepa L,Miglino M,Muner P,Damasio E,Gobbi M

    更新日期：2001-06-01 00:00:00

  • Early perfusion changes in patients with recurrent high-grade brain tumor treated with Bevacizumab: preliminary results by a quantitative evaluation.

    abstract：BACKGROUND:To determine whether early monitoring of the effects of bevacizumab in patients with recurrent high-grade gliomas, by a Perfusion Computed Tomography (PCT), may be a predictor of the response to treatment assessed through conventional MRI follow-up. METHODS:Sixteen patients were enrolled in the present stud...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 临床试验,杂志文章

    doi：10.1186/1756-9966-31-33

    authors： Vidiri A,Pace A,Fabi A,Maschio M,Latagliata GM,Anelli V,Piludu F,Carapella CM,Giovinazzo G,Marzi S

    更新日期：2012-04-11 00:00:00

  • LncRNA XIST/miR-34a axis modulates the cell proliferation and tumor growth of thyroid cancer through MET-PI3K-AKT signaling.

    abstract：BACKGROUND:Thyroid cancer is one of the most prevalent malignancies in endocrine system. Further understanding and revealing the molecular mechanism underlying thyroid cancer are indispensable for the development of effective diagnosis and treatments. In the present study, we attempted to provide novel basis for target...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-018-0950-9

    authors： Liu H,Deng H,Zhao Y,Li C,Liang Y

    更新日期：2018-11-21 00:00:00

  • miR-539 inhibits prostate cancer progression by directly targeting SPAG5.

    abstract：BACKGROUND:We conducted multiple microarray datasets analyses from clinical and xenograft tumor tissues to search for disease progression-driving oncogenes in prostate cancer (PCa). Sperm-associated antigen 5 (SPAG5) attracted our attention. SPAG5 was recently identified as an oncogene participating in lung cancer and ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-016-0337-8

    authors： Zhang H,Li S,Yang X,Qiao B,Zhang Z,Xu Y

    更新日期：2016-04-01 00:00:00

  • A central role for MeCP2 in the epigenetic repression of miR-200c during epithelial-to-mesenchymal transition of glioma.

    abstract：BACKGROUND:The epithelial-to-mesenchymal transition (EMT) has been linked to the regulation of glioma progression. However, the underlying signaling mechanisms that regulate EMT are poorly understood. METHODS:Quantitative real-time PCR (RT-qPCR) and western blot were performed to detect the expression of MeCP2 in glio...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-019-1341-6

    authors： Bian E,Chen X,Xu Y,Ji X,Cheng M,Wang H,Fang Z,Zhao B

    更新日期：2019-08-20 00:00:00

  • PCBP1 depletion promotes tumorigenesis through attenuation of p27Kip1 mRNA stability and translation.

    abstract：BACKGROUND:Poly C Binding Protein 1 (PCBP1) is an RNA-binding protein that binds and regulates translational activity of subsets of cellular mRNAs. Depletion of PCBP1 is implicated in various carcinomas, but the underlying mechanism in tumorigenesis remains elusive. METHODS:We performed a transcriptome-wide screen to ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-018-0840-1

    authors： Shi H,Li H,Yuan R,Guan W,Zhang X,Zhang S,Zhang W,Tong F,Li L,Song Z,Wang C,Yang S,Wang H

    更新日期：2018-08-07 00:00:00

  • Obstructive biliary symptomatology as the first sign of HIV-infection.

    abstract：:A 41-year-old white homosexual man presented with epigastric pain and jaundice. Physical examination showed enlargement of bilateral axillar and left inguinal lymph node, while ERCP and a CT scan suggested interruption of bile flow in the intrapancreatic tract of the common bile duct. An endoprosthesis was positioned ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： De Toma G,Codacci-Pisanelli M,Nicolanti V,Plocco M,Caterino S,Perno CF

    更新日期：1999-12-01 00:00:00

  • Fusion between homologous cells, modified epigenetic environment and possible cell cancerization.

    abstract：:For a long time now we have been putting forwards the hypothesis that, when two cells belonging to different tissues of the same organism fuse, the nucleous/nuclei of the resulting "fused" cell could lay into a cytoplasm possibly containing factors influencing the activation or deactivation of genes able to interfere ...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：

    authors： Sebastiani M,Soldati V

    更新日期：2002-06-01 00:00:00

  • Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL.

    abstract：BACKGROUND:Survival of adults with B-Acute Lymphoblastic Leukemia requires accurate risk stratification of patients in order to provide the appropriate therapy. Contemporary techniques, using clinical and cytogenetic variables are incomplete for prognosis prediction. METHODS:To improve the classification of adult pati...

    journal_title：Journal of experimental & clinical cancer research : CR

    pub_type： 杂志文章

    doi：10.1186/s13046-017-0506-4

    authors： Cruz-Rodriguez N,Combita AL,Enciso LJ,Raney LF,Pinzon PL,Lozano OC,Campos AM,Peñaloza N,Solano J,Herrera MV,Zabaleta J,Quijano S

    更新日期：2017-02-28 00:00:00