Molecular structure of human seminal coagulum: the role of proteolysis.

Abstract:

:The techniques of SDS-polyacrylamide slab gel electrophoresis and a sensitive fluorescamine-based method were used to study autoproteolysis of human seminal coagulum at various pH. The major protein bands of 72 and 55 Kd were optimally degraded at pH 7.5. Degradation at pH 3.5 was preceded by a lag period of 30 min. When the seminal coagulum was incubated for 5 min at pH 7.5 and then adjusted to pH 3.5, degradation was more rapid and complete than at pH 3.5 only. Autoproteolytic degradation product of discreted sizes were obtained when compared with degradation in the presence of pronase.

journal_name

Andrologia

journal_title

Andrologia

authors

Boonsaeng V

doi

10.1111/j.1439-0272.1986.tb01771.x

subject

Has Abstract

pub_date

1986-05-01 00:00:00

pages

252-8

issue

3

eissn

0303-4569

issn

1439-0272

journal_volume

18

pub_type

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