Preliminary observations on steroid metabolism in isolated epithelum of guinea pig seminal vesicle.

Abstract:

:The metabolism of nine radioactively labeled steroids in the epithelium of the seminal vesicle of the mature guinea pig has been studied. The rapid assimilation and metabolism of these steroids demostrate the very active biochemical nature of this tissue. Based on the use of several thin-layer chromatography systems and comparison with the locations of known standards, the following have been disclosed. Testosterone was rapidly converted to dihydrostestosterone and androstanediol. The latter was the major metablolite of dihydrotestosterone and of androsterone. Androstenedione was readily converted to androstanedione, testosterone, and dihydrostestosterone, although it formed little androstanediol. Dehydroepiandrosterone was converted to small amounts of androstenediol and androstanediol. Pregnenolone was rapidly converted to an unidentified highly polar compound only. Progesterone was converted to 5alpha-pregnane-3beta-ol-20-one and its 3alpha-isomer. Dehydroepiandrosterone and progesterone were also significantly converted to unidentified highly polar compounds. The major metabolites of 17-hydroxyprogesterone co-chromatographed with standard androstanediol, testosterone, and unidentified metabolite possessing intermediate chromatographic mobility. In addition, 17-hydroxyprogesterone was converted to small amounts of compounds possessing identical Rf values as standard androstenedione and dihydrostestosterone. The identification of the products of 17-hydroxyprogesterone metabolism and their physiologic significance must await critical evaluation. Because of its homogeneity, isolated epithelium of guinea pig seminal vesicle shows promise as a tissue preparation for use in future studies that might elucidate the role(s) of individual androgens in secretory tissues of the male accessory sex organs. Our demonstration of extensive steroid interconversions in this tissue is a logical prerequisite to such studies. :Metabolism of 9 labeled steroids by epithelium of mature guinea pig seminal vesicles was analyzed by thin layer chromatography. Testosterone was rapidly converted to dihydrotestosterone, and dihydrotestosterone to its major metabolite androstanediol. Androstenedione yielded androstanedione, testosterone, and dihydrotestosterone, but produced little androstanediol. The epithelium made small amounts of androstenediol and androstanediol from dehydroepiandrosteone. Pregnenolone, dehydroepiandrosterone, and progesterone was metabolized to highly polar compounds. Progesterone was transformed to 5alpha-pregnane-3beta-ol-one and its 3 alpha-isomer. The major metabolites of 17-hydroxyprogesterone were androstanediol, testosterone, and an unknown with intermediate mobility, and minor products were androstenedione and dihydrotestosterone. Immature and mature acute castrated guinea pigs showed significant androgen metabolism in small scale experiments, although the tissues were not pure epithelium.

journal_name

Andrologia

journal_title

Andrologia

authors

Steer RC,Veneziale CM

doi

10.1111/j.1439-0272.1977.tb01274.x

keywords:

subject

Has Abstract

pub_date

1977-04-01 00:00:00

pages

141-54

issue

2

eissn

0303-4569

issn

1439-0272

journal_volume

9

pub_type

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