Abstract:
:The aim of the study was to investigate whether the promoter methylation of XRCC1 and ERCC2 genes is associated with sperm DNA fragmentation and chromatin condensation in idiopathic oligoasthenoteratozoospermic men. This study involved 77 infertile men with idiopathic oligoasthenoteratozoospermia and 51 normozoospermic controls. The methylight method, TUNEL assay and aniline blue staining were used for the evaluation of XRCC1 and ERCC2 genes' methylation, SDF and sperm chromatin condensation, respectively. SDF (p = .004) and XRCC1 methylation (p = .0056) were found to be significantly higher in men with idiopathic OAT than in the controls, while mature spermatozoa frequency was higher in controls as compared to infertile men (p < .0001). No significant association was found between SDF and methylation of XRCC1 and ERCC2 genes (p = .9277 and p = .8257, respectively). However, compared to the cut-off point obtained by receiver operating characteristic analysis, a significant association was found between SDF and XRCC1 methylation, positive and negative methylation groups, generated according to the cut-off value for XRCC1. XRCC1 methylation was found to have a significant effect on chromatin condensation (p = .0017). No significant difference was detected among ERCC2 methylation, male infertility and SDF. In conclusion, XRCC1 methylation may have a role in sperm chromatin condensation and idiopathic OAT.
journal_name
Andrologiajournal_title
Andrologiaauthors
Metin Mahmutoglu A,Gunes S,Asci R,Henkel R,Aydin Odoi
10.1111/and.13925subject
Has Abstractpub_date
2020-12-23 00:00:00pages
e13925eissn
0303-4569issn
1439-0272pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::We studied the protective effect of Satureja khuzestanica essential oil (SKEO) against damage caused by busulfan on testis in male mice. The NMRI mice (n = 40) were assigned to four groups including: G1: control, G2: treated with busulfan for 4 days (3.2 mg kg(-1)), G3: receive busulfan (4 days, 3.2 mg kg(-1)) and SKE...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12421
更新日期:2016-02-01 00:00:00
abstract::Partial priapism is a rare disorder in literature and generally described as a contusion or thrombosis of the cavernous body of the penis secondary to blunt trauma. Because of the rarity of disease, there is not much information about the treatment. Conservative management with nonsteroidal anti-inflammatory drug is o...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13510
更新日期:2020-04-01 00:00:00
abstract::Studies assessing the efficacy of intralesional verapamil injection in the treatment of Peyronie's disease have yielded mixed results. The purpose of this meta-analysis is to systematise the existing literature on the efficacy of intralesional verapamil injection when used in the treatment of Peyronie's disease. The t...
journal_title:Andrologia
pub_type: 杂志文章,meta分析
doi:10.1111/and.13388
更新日期:2019-11-01 00:00:00
abstract::The numbers of Sertoli and pre-meiotic germ cells in the developing rabbit testis were investigated as an initial step in determining the physiological meaning of the control of cell number in the testis by apoptosis. Sections were stained immunohistochemically for the detection of apoptotic cells and counterstained w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.2002.00464.x
更新日期:2002-02-01 00:00:00
abstract::Mast cells in the bilateral testicular biopsies of 30 patients with a 'mixed atrophy' of seminiferous tubules were analysed. Seven biopsies from vasectomized patients served as controls. With regard to their characteristic location within testicular tissue, two groups of mast cells could be distinguished, in both cont...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.1999.00287.x
更新日期:1999-07-01 00:00:00
abstract::The objective of this study was to evaluate pubertal onset and characteristics in Egyptian boys. Examination of schoolboys (9-18 years) included height (cm), penile length (cm) and testicular volume (ml). Pubertal onset was considered at gonadal (G) stage 2 (G2 indicated testicular volume from 4-8 ml). Out of 1,078 bo...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13192
更新日期:2019-03-01 00:00:00
abstract::This study investigated the impact of the administration of HAART and anti-Koch's, singly and in combination, on sexual competence and birth statistics. Adult male Wistar rats were randomised into distilled water-treated control, HAART-treated, anti-Koch's-treated and HAART + anti-Koch's-treated groups. The 56-day ora...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13951
更新日期:2021-01-05 00:00:00
abstract::Human ejaculates collected for in vitro procedures show variably rapid increases in osmolality, depending on enzymatic degradation of compounds. Changes in osmolality can affect cell functions due to the energy consuming processes needed to control cell volume. The aim was to examine the effects of a hypotonic challen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13859
更新日期:2020-12-01 00:00:00
abstract::The inactivation of ornithine decarboxylase, a polyamine biosynthetic enzyme, by alpha-difluoromethylornithine (alpha-DFMO) was studied in patients with chronic non-suppurative prostatitis. Partial clinical response and remission of gross hematospermia were observed after 18 g/day alpha-DFMO, when administered orally ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1981.tb00014.x
更新日期:1981-03-01 00:00:00
abstract::The lipid composition of the sperm membrane has been shown to exert a significant effect upon the functional quality of spermatozoa. We have studied fatty acid composition of the phospholipids in spermatozoa in asthenozoospermic and normozoospermic men and determined the ratio of polyunsaturated fatty acids (PUFAs) to...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2006.00735.x
更新日期:2006-10-01 00:00:00
abstract::Administration of indomethacin exhibited antigonadal action by inhibiting the Leydig cells, sex-accessories and spermatocyte-spermatid conversion process. Administration of PGE2 or PGF2 alpha had no effect on Leydig cell nuclear volume or accessory organ weights, but PGE2 had a restorative effect on the efficiency of ...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::Male infertility is a major health problem worldwide. We investigated a possible association between leptin, obesity, hormonal interplay and male infertility. This cross-sectional study of 313 males (178 infertile and 135 fertile) was carried out in 2017. The subjects were categorised by body mass index (BMI) and body...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13147
更新日期:2019-02-01 00:00:00
abstract::This study investigated the effects of rutin against reproductive damage caused by toxic mercury in male rats. Thirty-five Sprague Dawley rats were used. Control group was injected with saline for 7 days. The rutin-100 group received 100 mg/kg/b.w. rutin for 7 days. Mercuric chloride (HgCl2 ) group received 1.23 mg/kg...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13524
更新日期:2020-04-01 00:00:00
abstract::This study was a 12-week double blind placebo-controlled, randomized, parallel trial in which active treatment with different doses of Maca Gelatinizada was compared with placebo. The study aimed to demonstrate if effect of Maca on subjective report of sexual desire was because of effect on mood or serum testosterone ...
journal_title:Andrologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1439-0272.2002.00519.x
更新日期:2002-12-01 00:00:00
abstract::Pre- and postdexamethasone triiodothyronine (T3), thyroxine (T4), and TSH levels of thirteen patients with psychogenic sexual dysfunction and thirteen controls were studied. Patients showed lowered T4 levels in comparison with the control group whereas T3 and TSH levels did not differ significantly. Dexamethasone had ...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::5alpha-Dihydrotestosterone (DHT), the most powerful naturally occurring androgen, is commercially available since 1982 as a gel. In view of its considerably higher biopotency (three to six times) than of testosterone, side effects, particularly on the main target organ of androgens, the prostate, are anticipated. In f...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.2007.00786.x
更新日期:2007-12-01 00:00:00
abstract::The study investigated the association between lower urinary tract symptoms (LUTS) and sexual dysfunction in ageing men. It was a cross-sectional study in an unselected consecutive sample of 398 men aged >40 years attending a urology clinic. LUTS and sexual function were assessed by validated symptom scales, including...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2008.00876.x
更新日期:2008-12-01 00:00:00
abstract::The objective was to determine effects of gallic acid (GA) and carnosic acid (CA), present in carob pods and rosemary extract respectively, on frozen-thawed ram spermatozoa. Thirty ejaculates were collected from five Merino rams, pooled, diluted in Tris-based extender and divided into five equal portions containing: 0...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13393
更新日期:2019-11-01 00:00:00
abstract::The aim of this study was to investigate possible ultrasound seminal vesicle (SV) changes in infertile patients with 'hypertrophic-congestive' (HCUF) or 'fibro-sclerotic' (FSUF) ultrasound form of male accessory gland infection (MAGI) after prolonged administration of tadalafil (TAD), a selective phosphodiesterase-5 i...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12027
更新日期:2013-12-01 00:00:00
abstract::Infertility occurs in up to 54% of men with bilateral undescended testes. Orchiectomy is considered the best therapeutic approach, especially when cryptorchidism is diagnosed in adulthood, due to a high risk of malignancy. A 33-year-old man was referred with a clinical presentation of empty scrotum and an ultrasonogra...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12936
更新日期:2018-03-01 00:00:00
abstract::Chronic prostatitis/chronic pelvic pain syndrome (CP⁄CPPS) is the prostate gland inflammation characterised as genitourinary pain in the pelvic region. The rat experimental autoimmune prostatitis (EAP) was achieved to mimic CP⁄CPPS. The expressions of transient receptor potential vanilloid 1 (TRPV1) in the prostate, b...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13302
更新日期:2019-09-01 00:00:00
abstract::When washed human sperm were incubated in a modified Krebs-Ringer buffer solution in the absence of exogenous metabolizable substrates at 30 degrees C they maintained progressive motility for at least six hours. Under these conditions the spermatozoa apparently utilize endogenous substrates but addition of exogenous s...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1986.tb01792.x
更新日期:1986-07-01 00:00:00
abstract::Oestrus urine was proved as a potential endocrine modulator in alleviating the toxicity induced by 3-methylcholanthrene (3-MC) in male rats. We, in this study, aimed to prove the attributing potential of toxicity alleviation to squalene, an oestrus-specific pheromone in rats. A single dose of 3-methylcholanthrene (25 ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13117
更新日期:2018-12-01 00:00:00
abstract::The aim of this study was to evaluate the effect of Lepidium meyenii (maca) in chemically and physically subfertile mice. After 35 days, the following groups of mice were evaluated: control, sham, chemical subfertility, chemical subfertility-maca-supplemented, physical subfertility, physical subfertility-maca-suppleme...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12682
更新日期:2016-10-01 00:00:00
abstract::The possible physiological role of testicular corticotropin-releasing factor (CRF) in the regulation of testicular functions was studied in neonatal rats. Two microlitres of anti-CRF-antiserum (dilution: 1:10 or 1:100) was injected intratesticularly to 5 d-old rats with two testes and to hemicastrates. Five days after...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1993.tb02711.x
更新日期:1993-07-01 00:00:00
abstract::Our aim was to compare peripheral blood and seminal fluid serum amyloid A (SAA) protein levels in men classified on the basis of sperm concentration and investigate whether SAA protein is an important marker of male infertility. A total of 74 first-attempt IVF male partners of infertile couples classified as azoosperm...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12491
更新日期:2016-08-01 00:00:00
abstract::In this study, the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvumDNA were investigated using real-time multiplex PCR detection kit in the urine samples of 50 patients who presented to the infertili...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13127
更新日期:2018-12-01 00:00:00
abstract::Varicocele is one of causes of the declined sperm quality and low sperm production, which can lead to infertility in males. There are several experimental and epidemiological findings which support the idea that inflammatory mechanisms play an essential role in varicocele pathogenesis. Besides, in this pathological st...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12685
更新日期:2017-08-01 00:00:00
abstract::Between 1994 and 2010, a total of 123 patients with obstructive azoospermia due to aplasia of vas deferens (CAVD) were surgically treated. In 110 patients, the condition was bilateral (CBAVD), 13 men had unilateral aplasia (CUAVD), and 10 patients additionally had aplasia of one kidney. All patients underwent CFTR gen...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2012.01281.x
更新日期:2012-10-01 00:00:00
abstract::Iatrogenic obstruction of the vas deferens within the inguinal canal can be managed by direct on-site vasovasostomy. However, in cases with large defect of the vas, the anastomosis may be under tension. Dissecting through the site of a previous hernia repair is tedious, and may lead to recurrence of the hernia. The pr...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2004.00624.x
更新日期:2004-10-01 00:00:00