Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50.

abstract：:"Stoppers" are a class of Neurospora crassa extranuclear mutants characterized by gross deficiencies of cytochromes b and aa3 and an unusual growth phenotype which involves irregular periods of growth andnongrowth. In the present work, mtDNAs from all four stopper mutants were found to contain deletions or insertions ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bertrand H,Collins RA,Stohl LL,Goewert RR,Lambowitz AM

更新日期：1980-10-01 00:00:00

abstract：:Escherichia coli cells treated with toluene replicate DNA when they are provided with deoxyribonucleoside 5'-triphosphates, ATP, Mg(++), and K(+). However, when deoxycytidine 5'-triphosphate is replaced by hydroxymethyl deoxycytidine 5'-triphosphate, incorporation of nucleotides into acid-precipitable material by tolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleischman RA,Richardson CC

更新日期：1971-10-01 00:00:00

abstract：:Alternative splicing is critical for diversifying eukaryotic proteomes, but the rules governing and coordinating splicing events among multiple alternate splice sites within individual genes are not well understood. We developed a quantitative PCR-based strategy to quantify the expression of the 12 transcripts encoded...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glauser DA,Johnson BE,Aldrich RW,Goodman MB

更新日期：2011-12-20 00:00:00

abstract：:Aspergillus fumigatus is commonly associated with allergic bronchopulmonary aspergillosis in patients with severe asthma in which chronic airway neutrophilia predicts a poor outcome. We were able to recapitulate fungus-induced neutrophilic airway inflammation in a mouse model in our efforts to understand the underlyin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fei M,Bhatia S,Oriss TB,Yarlagadda M,Khare A,Akira S,Saijo S,Iwakura Y,Fallert Junecko BA,Reinhart TA,Foreman O,Ray P,Kolls J,Ray A

更新日期：2011-03-29 00:00:00

abstract：:Cell couples have been formed by mixing an antigen- and Ia-specific cloned helper T-cell line with a B-cell hybridoma presenting the antigen. By immunofluorescence observations, we have shown that the microtubule-organizing center (MTOC) inside the helper T cell, but not in the bound antigen-presenting cell, becomes o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kupfer A,Swain SL,Janeway CA Jr,Singer SJ

更新日期：1986-08-01 00:00:00

abstract：:Fragment screens for new ligands have had wide success, notwithstanding their constraint to libraries of 1,000-10,000 molecules. Larger libraries would be addressable were molecular docking reliable for fragment screens, but this has not been widely accepted. To investigate docking's ability to prioritize fragments, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teotico DG,Babaoglu K,Rocklin GJ,Ferreira RS,Giannetti AM,Shoichet BK

更新日期：2009-05-05 00:00:00

abstract：:Adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) deficiency is one cause of the genetic disease severe combined immunodeficiency. To identify mutations responsible for ADA deficiency, we synthesized cDNAs to ADA mRNAs from two cell lines, GM2756 and GM2825A, derived from ADA-deficient immunodeficient pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akeson AL,Wiginton DA,States JC,Perme CM,Dusing MR,Hutton JJ

更新日期：1987-08-01 00:00:00

abstract：:The Mauer mandible, holotype of Homo heidelbergensis, was found in 1907 in fluvial sands deposited by the Neckar River 10 km southeast of Heidelberg, Germany. The fossil is an important key to understanding early human occupation of Europe north of the Alps. Given the associated mammal fauna and the geological context...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner GA,Krbetschek M,Degering D,Bahain JJ,Shao Q,Falguères C,Voinchet P,Dolo JM,Garcia T,Rightmire GP

更新日期：2010-11-16 00:00:00

abstract：:The major protein constituent of human plasma high density lipoproteins has been isolated and its complete amino-acid sequence determined. The protein, designated apolipoprotein-glutamine-I by the presence of carboxyl-terminal glutamine, is a single polypeptide chain of 245 amino-acid residues, including three residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baker HN,Delahunty T,Gotto AM Jr,Jackson RL

更新日期：1974-09-01 00:00:00

abstract：:Achieving label-free, molecular-specific imaging with high spatial resolution in deep tissue is often considered the grand challenge of optical imaging. To accomplish this goal, significant optical scattering in tissues has to be overcome while achieving molecular specificity without resorting to extrinsic labeling. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yakovlev VV,Zhang HF,Noojin GD,Denton ML,Thomas RJ,Scully MO

更新日期：2010-11-23 00:00:00

abstract：:The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) is induced as part of the IFN response in mammals and acts to shut down protein synthesis by the phosphorylation of eukaryotic initiation factor 2alpha (eIF2alpha). In fish, a PKR-like kinase activity has been detected, but the enzyme responsible has elude...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothenburg S,Deigendesch N,Dittmar K,Koch-Nolte F,Haag F,Lowenhaupt K,Rich A

更新日期：2005-02-01 00:00:00

abstract：:Classical genetic selection was combined with site-directed mutagenesis to study bacteriophage T4 DNA polymerase 3'----5' exonuclease activity. A mutant DNA polymerase with very little (less than or equal to 1%) 3'----5' exonuclease activity was generated. In vivo, the 3'----5' exonuclease-deficient DNA polymerase pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reha-Krantz LJ,Stocki S,Nonay RL,Dimayuga E,Goodrich LD,Konigsberg WH,Spicer EK

更新日期：1991-03-15 00:00:00

abstract：:Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 microM are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthesis. In the abse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JB

更新日期：1983-10-01 00:00:00

abstract：:We describe a strategy to "revive" putatively pathogenic T cells from frozen specimens of human inflammatory target organs. To distinguish pathogenic from irrelevant bystander T cells, we focused on cells that were (i) clonally expanded and (ii) in direct morphological contact with a target cell. Using CDR3 spectratyp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seitz S,Schneider CK,Malotka J,Nong X,Engel AG,Wekerle H,Hohlfeld R,Dornmair K

更新日期：2006-08-08 00:00:00

abstract：:Over the past decade or more, contradictory evidence of Martian climate, indicating that surface temperatures seldom if ever approach the melting point of water at midlatitudes, and geomorphic features, consistent with liquid flows at these same latitudes, have proven difficult to reconcile. In this article, we demons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shinbrot T,Duong NH,Kwan L,Alvarez MM

更新日期：2004-06-08 00:00:00

abstract：:The cingulate gyri in 37 subjects with and without early dementia of the Alzheimer type (DAT) were studied by using MRI at 1.0 mm3 isotropic resolution. Groups were segregated into young controls (n = 10), age-matched normal controls (n = 10), very mild DAT (n = 8), and mild DAT (n = 9). By using automated Bayesian se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller MI,Hosakere M,Barker AR,Priebe CE,Lee N,Ratnanather JT,Wang L,Gado M,Morris JC,Csernansky JG

更新日期：2003-12-09 00:00:00

abstract：:A controversial topic in the recent climate modeling literature is the fashion in which metastable low-frequency regimes in the atmosphere occur despite nearly Gaussian statistics for these planetary waves. Here a simple 57-mode paradigm model for such metastable atmospheric regime behavior is introduced and analyzed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majda AJ,Franzke CL,Fischer A,Crommelin DT

更新日期：2006-05-30 00:00:00

abstract：:Eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of dead...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Dijk E,Le Hir H,Séraphin B

更新日期：2003-10-14 00:00:00

abstract：:Human cDNA clones encoding a structure-specific recognition protein, SSRP1, that binds specifically to DNA modified with cisplatin have been isolated and characterized. The SSRP1 gene maps to human chromosome 11q12. The cDNA clones, obtained by using partial-length cDNAs described previously, predict an 81-kDa protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruhn SL,Pil PM,Essigmann JM,Housman DE,Lippard SJ

更新日期：1992-03-15 00:00:00

abstract：:Achieving long-term pancellular expression of a transferred gene at therapeutic level in a given hematopoietic lineage remains an important goal of gene therapy. Advances have recently been made in the genetic correction of the hemoglobinopathies by means of lentiviral vectors and large locus control region (LCR) deri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Imren S,Payen E,Westerman KA,Pawliuk R,Fabry ME,Eaves CJ,Cavilla B,Wadsworth LD,Beuzard Y,Bouhassira EE,Russell R,London IM,Nagel RL,Leboulch P,Humphries RK

更新日期：2002-10-29 00:00:00

abstract：:Marriage in many traditional societies often concerns the institutionalized exchange of reproductive partners among groups of kin. Such exchanges most often involve cross-cousins-marriage with the child of a parent's opposite-sex sibling-but it is unclear who benefits from these exchanges. Here we analyze the fitness ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chagnon NA,Lynch RF,Shenk MK,Hames R,Flinn MV

更新日期：2017-03-28 00:00:00

abstract：:A low molecular weight inhibitor of cell-free protein synthesis effective at subnanomolar concentrations is formed on incubation of cytoplasmic extracts from interferon-treated cells with double-stranded RNA and ATP. It can be conveniently synthesized by incubating a poly(I).poly(C)-Sepharose-bound enzyme fraction fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kerr IM,Brown RE

更新日期：1978-01-01 00:00:00

abstract：:Molecular clocks have been used to date the divergence of humans and chimpanzees for nearly four decades. Nonetheless, this date and its confidence interval remain to be firmly established. In an effort to generate a genomic view of the human-chimpanzee divergence, we have analyzed 167 nuclear protein-coding genes and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumar S,Filipski A,Swarna V,Walker A,Hedges SB

更新日期：2005-12-27 00:00:00

abstract：:Sympathetic ganglia and some peripheral tissues of adult guinea pig and cat were analyzed by the indirect immunofluorescence technique with antisera to catecholamine-synthesizing enzymes and some peptides. In the guinea pig, noradrenergic neurons could be subdivided into three populations containing respectively (i) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lundberg JM,Hökfelt T,Anggård A,Terenius L,Elde R,Markey K,Goldstein M,Kimmel J

更新日期：1982-02-01 00:00:00

abstract：:We have isolated a lymphoid cell line, MDS, from the pleural exudate of a patient with chronic myelomonocytic leukemia. The cells are biphenotypic, containing various T-cell and myeloid markers, and are surface negative for CD4 and CD8 but have low CD4 mRNA. The cells grow in suspension with a doubling time of 15 hr, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banerjee R,Bekesi JG,Tarcsafalvi A,Sperber K,Deak G,Choi HS,Paronetto F,Holland JF,Acs G

更新日期：1992-11-01 00:00:00

abstract：:Plants sense phosphate (Pi) deficiency and initiate signaling that controls adaptive responses necessary for Pi acquisition. Herein, evidence establishes that AtSIZ1 is a plant small ubiquitin-like modifier (SUMO) E3 ligase and is a focal controller of Pi starvation-dependent responses. T-DNA insertional mutated allel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miura K,Rus A,Sharkhuu A,Yokoi S,Karthikeyan AS,Raghothama KG,Baek D,Koo YD,Jin JB,Bressan RA,Yun DJ,Hasegawa PM

更新日期：2005-05-24 00:00:00

abstract：:We have isolated, by molecular cloning and genetic complementation of a listeriolysin-negative mutant, a gene required for the expression of this virulence factor in Listeria monocytogenes. The mutant strain SLCC53, which was nonhemolytic and avirulent, harbored a deletion of 450 base pairs located approximately 1500 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leimeister-Wächter M,Haffner C,Domann E,Goebel W,Chakraborty T

更新日期：1990-11-01 00:00:00

abstract：:Microviscosity (n) of the cell membrane lipid layer was determined in synchronized C1300 mouse neuroblastoma cells (clone Neuro-2A) by fluorescence polarization of 1,6-diphenystratum. The determined n value was maximal in mitosis, decreased markedly in the G1 phase, remained constant at a low level during the S phase,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Laat SW,van der Saag PT,Shinitzky M

更新日期：1977-10-01 00:00:00

abstract：:Hatchlings of the painted turtle (Chrysemys picta marginata) are unique as the only reptile and highest vertebrate life form known to tolerate the natural freezing of extracellular body fluids during winter hibernation. Turtles survived frequent exposures to temperatures as low as -6 degrees C to -8 degrees C in their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Storey KB,Storey JM,Brooks SP,Churchill TA,Brooks RJ

更新日期：1988-11-01 00:00:00

abstract：:The chimaeric protein repressor-galactosidase, in which fully active lac repressor is covalently linked to the active enzyme beta-galactosidase, was used as a system for probing the quaternary structure of lac repressor. Electron micrographs revealed repressor-galactosidase to be a tetrameric aggregate. When lac repre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kania J,Brown DT

更新日期：1976-10-01 00:00:00