Abstract:
:Recombination in mammalian cells is thought to involve both reciprocal and nonreciprocal modes of exchange, although rigorous proof is lacking due to the inability to recover all products of an exchange. To investigate further the relationship between these modes of exchange, we have analyzed intrachromosomal recombination between duplicated herpes simplex virus thymidine kinase (HSV tk) mutant alleles arranged as inverted repeats in cultured mouse L cells. In crosses between inverted repeats, a single intrachromatid reciprocal exchange leads to inversion of the sequence between the crossover sites and recovery of both genes involved in the event. The majority of recombinant products do not display such inversion and are thus consistent with a nonreciprocal mode of recombination (gene conversion). The remaining products display the sequence inversion predicted for intrachromatid reciprocal exchange. In light of the fact that intrachromatid exchanges occur, the rarity of intrachromatid double reciprocal exchanges strengthens the interpretation that the majority of events in this and previous investigations involve gene conversion. Furthermore, in accord with prediction, one-third of the reciprocal recombinants (inversions) display associated gene conversion. This association suggests that reciprocal and nonreciprocal modes of exchange are mechanistically related in mammalian cells. Finally, the occurrence of inversion recombinants suggests that intrachromosomal recombination can be a conservative (nondestructive) process.
journal_name
Geneticsjournal_title
Geneticsauthors
Bollag RJ,Liskay RMsubject
Has Abstractpub_date
1988-05-01 00:00:00pages
161-9issue
1eissn
0016-6731issn
1943-2631journal_volume
119pub_type
杂志文章相关文献
GENETICS文献大全abstract::As the dynamic nature of progenitor genomes accompanies the speciation by interspecific hybridization, the extraction of the constituent subgenome(s) from a natural allopolyploid species of long history and then restitution of the progenitor(s) provides the unique opportunity to study the genome evolution and interpla...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.190967
更新日期:2016-11-01 00:00:00
abstract::Transcriptional silencing involves the formation of specialized repressive chromatin structures. Previous studies have shown that the histone H3-H4 chaperone known as chromatin assembly factor 1 (CAF-1) contributes to transcriptional silencing in yeast, although the molecular basis for this was unknown. In this work w...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.054783
更新日期:2006-06-01 00:00:00
abstract::Sister-chromatid exchanges (SCE) were studied in allium cepa L. meristematic cells at the second and third divisions after BrdUrd-substitution during just the first or during the second and third cycles, respectively. The observed SCE nonreciprocal/reciprocal ratios detected at the third division in both experiments, ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::Nonallelic gene conversion has been proposed as a major force in homogenizing the sequences of paralogous genes. In this work, we investigate the extent and characteristics of gene conversion among gene families in nine species of the genus Drosophila. We carried out a genome-wide study of 2855 gene families (includin...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.115444
更新日期:2010-05-01 00:00:00
abstract::Yeast cells subjected to a reversible thermal arrest of meiosis yielded progressively fewer spores per ascus as the arrest was extended. Dissection of two-spored asci by a newly developed method that prevents selection of false asci revealed that the spores were not a random sample of the haploid meiotic products. Mos...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::Two Podospora mutants carrying mutations modE and modF were persumed to be quiescent defective, because, when grown under glucose limitation, they differed from the wild-type strain in an excess of dry weight production and a reduction of cell survival. New insight on the action of modE and modF mutations was provided...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Gastrulation is fundamental to the development of multicellular animals. Along with neurulation, gastrulation is one of the major processes of morphogenesis in which cells or whole tissues move from the surface of an embryo to its interior. Cell internalization mechanisms that have been discovered to date in Caenorhab...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.119.300240
更新日期:2020-02-01 00:00:00
abstract::We have analyzed the requirements for the multi sex combs (mxc) gene during development to gain further insight into the mechanisms and developmental processes that depend on the important trans-regulators forming the Polycomb group (PcG) in Drosophila melanogaster. mxc is allelic with the tumor suppressor locus letha...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Methods for inference and interpretation of evolutionary quantitative genetic parameters, and for prediction of the response to selection, are best developed for traits with normal distributions. Many traits of evolutionary interest, including many life history and behavioral traits, have inherently nonnormal distribu...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.186536
更新日期:2016-11-01 00:00:00
abstract::To identify in vivo pathways that compensate for impaired proliferating cell nuclear antigen (PCNA or Pol30p in yeast) activity, we performed a synthetic lethal screen with the yeast pol30-104 mutation. We identified nine mutations that display synthetic lethality with pol30-104; three mutations affected the structura...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Electron microscope serial section reconstruction analysis of all zygotene-pachytene nuclei of meiotic cells from three wild-type germaria (a subunit of the ovary containing the early meiotic stages arrayed in temporal developmental sequence) of Drosophila melanogaster females corroborates and extends earlier observat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Genomic prediction methods based on multiple markers have potential to include nonadditive effects in prediction and analysis of complex traits. However, most developments assume a Hardy-Weinberg equilibrium (HWE). Statistical approaches for genomic selection that account for dominance and epistasis in a general conte...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.199406
更新日期:2017-07-01 00:00:00
abstract::The breeding systems of many organisms are cryptic and difficult to investigate with observational data, yet they have profound effects on a species' ecology, evolution, and genome organization. Genomic approaches offer a novel, indirect way to investigate breeding systems, specifically by studying the transmission of...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.179879
更新日期:2015-11-01 00:00:00
abstract::We examined linkage relationships for RecBC-mediated recombination in lytic cycle crosses of lambda phages bearing two cohesive end sites (cos) oriented in the same direction. The relationships obtained imply that a given recombinant tends to be packaged from the cos site that is the nearer one to the right of the exc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
abstract::The Collaborative Cross (CC) was designed to facilitate rapid gene mapping and consists of hundreds of recombinant inbred lines descended from eight diverse inbred founder strains. A decade in production, it can now be applied to mapping projects. Here, we provide a proof of principle for rapid identification of major...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.163014
更新日期:2014-09-01 00:00:00
abstract::The evidence in support of two types of alternate disjunction in translocation heterozygotes from the German cockroach, Blattella germanica (L.), is reviewed. When unique cytomorphological features occur in the translocation figure, the two types of alternate disjunction are identifiable regardless of the angle of obs...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::Genes expressed in testes are critical to male reproductive success, affecting spermatogenesis, sperm competition, and sperm-egg interaction. Comparing the evolution of testis proteins at different taxonomic levels can reveal which genes and functional classes are targets of natural and sexual selection and whether th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.085902
更新日期:2008-08-01 00:00:00
abstract::The Mus101 family of chromosomal proteins, identified initially in Drosophila, is widely conserved and has been shown to function in a variety of DNA metabolic processes. Such functions include DNA replication, DNA damage repair, postreplication repair, damage checkpoint activation, chromosome stability, and chromosom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.036137
更新日期:2005-03-01 00:00:00
abstract::The walls of Aspergillus nidulans conidia contain a green pigment that protects the spores from damage by ultraviolet light. At least two genes, wA and yA, are required for pigment synthesis: yA mutants produce yellow spores, wA mutants produce white spores, and wA mutations are epistatic to yA mutations. We cloned wA...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::The transcription factors WHITE COLLAR-1 (WC-1) and WHITE COLLAR-2 (WC-2) interact to form a heterodimeric complex (WCC) that is essential for most of the light-mediated processes in Neurospora crassa. WCC also plays a distinct non-light-related role as the transcriptional activator in the FREQUENCY (FRQ)/WCC feedback...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Mapping markers from linkage data continues to be a task performed in many genetic epidemiological studies. Data collected in a study may be used to refine published map estimates and a study may use markers that do not appear in any published map. Furthermore, inaccuracies in meiotic maps can seriously bias linkage f...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.042705
更新日期:2005-10-01 00:00:00
abstract::The primary signal for Drosophila sex determination is the number of X chromosomes relative to the number of sets of autosomes. The present report shows that the numerator of this X/A signal appears to be determined by the cumulative dose of a relatively limited number of discrete X-linked genetic elements, two of whi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::One crossover point between a pair of homologous chromosomes in meiosis appears to interfere with occurrence of another in the neighborhood. It has been revealed that Drosophila and Neurospora, in spite of their large difference in the frequency of crossover points, show very similar plots of coincidence-a measure of ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Ras1 GTPase is the Schizosaccharomyces pombe homolog of the mammalian Ha-Ras proto-oncoprotein. Ras1 interacts with Scd1 (aka Ral1), a presumptive guanine nucleotide exchange factor for Cdc42sp, to control organization of the cytoskeleton. In this study, we demonstrated that the scd1 deletion (scd1Delta) induced hyper...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Restriction site-associated DNA sequencing or genotyping-by-sequencing (GBS) approaches allow for rapid and cost-effective discovery and genotyping of thousands of single-nucleotide polymorphisms (SNPs) in multiple individuals. However, rigorous quality control practices are needed to avoid high levels of error and bi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.169052
更新日期:2014-11-01 00:00:00
abstract::We developed stochastic population genetic theory for mitochondrial and chloroplast genes, using an infinite alleles model appropriate for molecular genetic data. We considered the effects of mutation, random drift, and migration in a finite island model on selectively neutral alleles. Recurrence equations were obtain...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::Linkage relationships of nine enzyme loci; aconitase (Acon ), esterase (Est), glucosephosphate isomerase A and B ( Gpi), glycerate-2-dehydrogenase (G2dh), malic enzyme (Me ), phosphoglycerate kinase (Pgk), phosphoglucomutase (Pgm ) and superoxide dismutase (Sod), were investigated in sunfishes (Lepomis, Centrarchidae)...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Data are presented for 2,393 progeny from a number of crosses related to a study in ecological genetics of the Burnsi and Kandiyohi polymorphisms in natural populations of the leopard frog, Rana pipiens. No significant differences in viability were found between wild-type homozygotes (+/+) and Burnsi heterozygotes (B/...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-01-01 00:00:00
abstract::A number of fundamental processes comprise the cell division cycle, including spindle formation, chromosome segregation, and cytokinesis. Our current understanding of these processes has benefited from the isolation and analysis of mutants, with the meiotic divisions in the male germline of Drosophila being particular...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::The third hypervariable (V3) region of the HIV-1 gp120 protein is responsible for many aspects of viral infectivity. The tertiary structure of the V3 loop seems to influence the coreceptor usage of the virus, which is an important determinant of HIV pathogenesis. Hence, the information about preferred conformations of...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051508
更新日期:2006-03-01 00:00:00