当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR.

Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR.

Abstract：

:Gaussia luciferase (GLuc) is a small luciferase (18.2 kDa; 168 residues) and is thus attracting much attention as a reporter protein, but the lack of structural information is hampering further application. Here, we report the first solution structure of a fully active, recombinant GLuc determined by heteronuclear multidimensional NMR. We obtained a natively folded GLuc by bacterial expression and efficient refolding using a Solubility Enhancement Petide (SEP) tag. Almost perfect assignments of GLuc's 1H, 13C and 15N backbone signals were obtained. GLuc structure was determined using CYANA, which automatically identified over 2500 NOEs of which > 570 were long-range. GLuc is an all-alpha-helix protein made of nine helices. The region spanning residues 10-18, 36-81, 96-145 and containing eight out of the nine helices was determined with a Cα-atom RMSD of 1.39 Å ± 0.39 Å. The structure of GLuc is novel and unique. Two homologous sequential repeats form two anti-parallel bundles made by 4 helices and tied together by three disulfide bonds. The N-terminal helix 1 is grabbed by these 4 helices. Further, we found a hydrophobic cavity where several residues responsible for bioluminescence were identified in previous mutational studies, and we thus hypothesize that this is a catalytic cavity, where the hydrophobic coelenterazine binds and the bioluminescence reaction takes place.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Wu N,Kobayashi N,Tsuda K,Unzai S,Saotome T,Kuroda Y,Yamazaki T

doi

10.1038/s41598-020-76486-4

subject

Has Abstract

pub_date

2020-11-18 00:00:00

pages

20069

issue

1

issn

2045-2322

pii

10.1038/s41598-020-76486-4

journal_volume

10

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium.

    abstract：:For augmentation or reconstruction of urinary bladder after cystectomy, bladder urothelium derived from human induced pluripotent stem cells (hiPSCs) has recently received focus. However, previous studies have only shown the emergence of cells expressing some urothelial markers among derivatives of hiPSCs, and no repo...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46848-8

    authors： Suzuki K,Koyanagi-Aoi M,Uehara K,Hinata N,Fujisawa M,Aoi T

    更新日期：2019-07-19 00:00:00

  • Unexpected Reaction Pathway for butyrylcholinesterase-catalyzed inactivation of "hunger hormone" ghrelin.

    abstract：:Extensive computational modeling and simulations have been carried out, in the present study, to uncover the fundamental reaction pathway for butyrylcholinesterase (BChE)-catalyzed hydrolysis of ghrelin, demonstrating that the acylation process of BChE-catalyzed hydrolysis of ghrelin follows an unprecedented single-st...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep22322

    authors： Yao J,Yuan Y,Zheng F,Zhan CG

    更新日期：2016-02-29 00:00:00

  • High-performance asymmetric optical transmission based on coupled complementary subwavelength gratings.

    abstract：:Asymmetric transmission (AT) devices are fundamental elements for optical computing and information processing. We here propose an AT device consisting of a pair of coupled complementary subwavelength gratings. Different from previous works, asymmetric dielectric environment is employed for unidirectional excitation o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-53586-4

    authors： Li S,Huang LR,Ling YH,Liu WB,Ba CF,Li HH

    更新日期：2019-11-19 00:00:00

  • Structural Evolution of Hydrothermally Derived Reduced Graphene Oxide.

    abstract：:Hydrothermal reduction is a promising approach for graphene oxide (GO) reduction since it is environmentally friendly, simple, and cost effective. We present a detailed study of structural changes occurring in graphene oxide during the reduction process. The correlations between the interlayer spacing, chemical states...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-25194-1

    authors： Huang HH,De Silva KKH,Kumara GRA,Yoshimura M

    更新日期：2018-05-01 00:00:00

  • Distinct forms of motion sensitivity impairments in Alzheimer's disease.

    abstract：:Motion sensitivity impairment in Alzheimer's disease (AD) is often characterized as elevated coherence threshold. An alternative way to measure motion sensitivity is the direction threshold, i.e., the minimal angle of motion direction that can be discriminated. So far, it is less clear whether and how the direction th...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48942-3

    authors： Liu Y,Feng D,Wang H

    更新日期：2019-09-10 00:00:00

  • Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules.

    abstract：:The chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. Considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. Mas...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep27974

    authors： Guyot N,Labas V,Harichaux G,Chessé M,Poirier JC,Nys Y,Réhault-Godbert S

    更新日期：2016-06-13 00:00:00

  • Loss of A20 in BM-MSCs regulates the Th17/Treg balance in Rheumatoid Arthritis.

    abstract：:Mesenchymal stem cells (MSCs) are multi-potent cells that are self-renewable and possess the potential to differentiate into multiple lineages. Several studies demonstrated that MSCs could regulate a Th17/Treg balance and could be a potential therapeutic target for Rheumatoid Arthritis (RA). A20 is highly expressed in...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18693-0

    authors： Feng Z,Zhai Y,Zheng Z,Yang L,Luo X,Dong X,Han Q,Jin J,Chen ZN,Zhu P

    更新日期：2018-01-11 00:00:00

  • Competing effect of spin-orbit torque terms on perpendicular magnetization switching in structures with multiple inversion asymmetries.

    abstract：:Current-induced spin-orbit torques (SOTs) in structurally asymmetric multilayers have been used to efficiently manipulate magnetization. In a structure with vertical symmetry breaking, a damping-like SOT can deterministically switch a perpendicular magnet, provided an in-plane magnetic field is applied. Recently, it h...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep23956

    authors： Yu G,Akyol M,Upadhyaya P,Li X,He C,Fan Y,Montazeri M,Alzate JG,Lang M,Wong KL,Khalili Amiri P,Wang KL

    更新日期：2016-04-06 00:00:00

  • Tumour Necrosis Factor-α Inhibition Improves Stroke Outcome in a Mouse Model of Rheumatoid Arthritis.

    abstract：:Rheumatoid Arthritis (RA) is a chronic inflammatory disorder where incidence and severity of myocardial infarction are increased. Data on the incidence and outcome of stroke are conflicting. Thus, we investigated outcome after Ischemia/Reperfusion (I/R) brain injury in a mouse model of RA and assessed for the role of ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-38670-z

    authors： Bonetti NR,Diaz-Cañestro C,Liberale L,Crucet M,Akhmedov A,Merlini M,Reiner MF,Gobbato S,Stivala S,Kollias G,Ruschitzka F,Lüscher TF,Beer JH,Camici GG

    更新日期：2019-02-18 00:00:00

  • Aseismic transient during the 2010-2014 seismic swarm: evidence for longer recurrence of M ≥ 6.5 earthquakes in the Pollino gap (Southern Italy)?

    abstract：:In actively deforming regions, crustal deformation is accommodated by earthquakes and through a variety of transient aseismic phenomena. Here, we study the 2010-2014 Pollino (Southern Italy) swarm sequence (main shock M W 5.1) located within the Pollino seismic gap, by analysing the surface deformation derived from Gl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-00649-z

    authors： Cheloni D,D'Agostino N,Selvaggi G,Avallone A,Fornaro G,Giuliani R,Reale D,Sansosti E,Tizzani P

    更新日期：2017-04-12 00:00:00

  • Disparate subcellular location of putative sortase substrates in Clostridium difficile.

    abstract：:Clostridium difficile is a gastrointestinal pathogen but how the bacterium colonises this niche is still little understood. Sortase enzymes covalently attach specific bacterial proteins to the peptidoglycan cell wall and are often involved in colonisation by pathogens. Here we show C. difficile proteins CD2537 and CD3...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08322-1

    authors： Peltier J,Shaw HA,Wren BW,Fairweather NF

    更新日期：2017-08-23 00:00:00

  • Men increase contributions to a public good when under sexual competition.

    abstract：:Why humans cooperate in large groups and with non-kin remains a puzzle for researchers across the natural and social sciences. Investigating whether cooperation is sexually selected could contribute to an understanding of the evolution of human cooperation. Competition for access to mates could indeed select for coope...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29819

    authors： Tognetti A,Dubois D,Faurie C,Willinger M

    更新日期：2016-07-14 00:00:00

  • Detection of smoking status from retinal images; a Convolutional Neural Network study.

    abstract：:Cardiovascular diseases are directly linked to smoking habits, which has both physiological and anatomical effects on the systemic and retinal circulations, and these changes can be detected with fundus photographs. Here, we aimed to 1- design a Convolutional Neural Network (CNN), using retinal photographs, to differe...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43670-0

    authors： Vaghefi E,Yang S,Hill S,Humphrey G,Walker N,Squirrell D

    更新日期：2019-05-09 00:00:00

  • Direct determination of three-phase contact line properties on nearly molecular scale.

    abstract：:Wetting phenomena in multi-phase systems govern the shape of the contact line which separates the different phases. For liquids in contact with solid surfaces wetting is typically described in terms of contact angle. While in macroscopic systems the contact angle can be determined experimentally, on the molecular scal...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep26111

    authors： Winkler PM,McGraw RL,Bauer PS,Rentenberger C,Wagner PE

    更新日期：2016-05-17 00:00:00

  • Batrachochytrium salamandrivorans (Bsal) not detected in an intensive survey of wild North American amphibians.

    abstract：:The salamander chytrid fungus (Batrachochytrium salamandrivorans [Bsal]) is causing massive mortality of salamanders in Europe. The potential for spread via international trade into North America and the high diversity of salamanders has catalyzed concern about Bsal in the U.S. Surveillance programs for invading patho...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-69486-x

    authors： Waddle JH,Grear DA,Mosher BA,Grant EHC,Adams MJ,Backlin AR,Barichivich WJ,Brand AB,Bucciarelli GM,Calhoun DL,Chestnut T,Davenport JM,Dietrich AE,Fisher RN,Glorioso BM,Halstead BJ,Hayes MP,Honeycutt RK,Hossack BR,Kle

    更新日期：2020-08-03 00:00:00

  • Reconstruction of the cropland cover changes in eastern China between the 10th century and 13th century using historical documents.

    abstract：:To evaluate and improve datasets of anthropogenic land cover change used in local and global climate models, great efforts were made to reconstruct historical land use, including the LandCover 6k project which dedicated to reconstructing human land use over the past 10,000 years. In this study, we utilized historical ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-31807-6

    authors： Li M,He F,Li S,Yang F

    更新日期：2018-09-10 00:00:00

  • Composite Membranes of Recombinant Silkworm Antimicrobial Peptide and Poly (L-lactic Acid) (PLLA) for biomedical application.

    abstract：:Antimicrobial peptides, produced by innate immune system of hosts in response to invading pathogens, are capable of fighting against a spectrum of bacteria, viruses, fungi, parasites and cancer cells. Here, a recombinant silkworm AMP Bmattacin2 from heterologous expression is studied, indicating a broad spectrum of an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31149

    authors： Li Z,Liu X,Li Y,Lan X,Leung PH,Li J,Li G,Xie M,Han Y,Lin X

    更新日期：2016-08-09 00:00:00

  • Publisher Correction: A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-57850-w

    authors： Stringer BW,Day BW,D'Souza RCJ,Jamieson PR,Ensbey KS,Bruce ZC,Lim YC,Goasdoué K,Offenhäuser C,Akgül S,Allan S,Robertson T,Lucas P,Tollesson G,Campbell S,Winter C,Do H,Dobrovic A,Inglis PL,Jeffree RL,Johns TG,Boy

    更新日期：2020-01-21 00:00:00

  • Automated detection of leakage in fluorescein angiography images with application to malarial retinopathy.

    abstract：:The detection and assessment of leakage in retinal fluorescein angiogram images is important for the management of a wide range of retinal diseases. We have developed a framework that can automatically detect three types of leakage (large focal, punctate focal, and vessel segment leakage) and validated it on images fr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep10425

    authors： Zhao Y,MacCormick IJ,Parry DG,Leach S,Beare NA,Harding SP,Zheng Y

    更新日期：2015-06-01 00:00:00

  • Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.

    abstract：:Misfolding and aggregation of prion protein (PrP) causes neurodegenerative diseases like Creutzfeldt-Jakob disease (CJD) and scrapie. Besides the consensus that spontaneous conversion of normal cellular PrPC into misfolded and aggregating PrPSc is the central event in prion disease, an alternative hypothesis suggests ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-39213-2

    authors： Czech A,Konarev PV,Goebel I,Svergun DI,Wills PR,Ignatova Z

    更新日期：2019-02-21 00:00:00

  • Fluorinated benzalkylsilane molecular rectifiers.

    abstract：:We report on the synthesis and electrical properties of nine new alkylated silane self-assembled monolayers (SAMs) - (EtO)3Si(CH2)nN = CHPhX where n = 3 or 11 and X = 4-CF3, 3,5-CF3, 3-F-4-CF3, 4-F, or 2,3,4,5,6-F, and explore their rectification behavior in relation to their molecular structure. The electrical proper...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38092

    authors： Lamport ZA,Broadnax AD,Harrison D,Barth KJ,Mendenhall L,Hamilton CT,Guthold M,Thonhauser T,Welker ME,Jurchescu OD

    更新日期：2016-11-29 00:00:00

  • Publisher Correction: BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-018-33869-y

    authors： Boel A,Steyaert W,De Rocker N,Menten B,Callewaert B,De Paepe A,Coucke P,Willaert A

    更新日期：2018-10-29 00:00:00

  • Plasma dipeptidyl-peptidase-4 activity is associated with left ventricular systolic function in patients with ST-segment elevation myocardial infarction.

    abstract：:Plasma dipeptidyl-peptidase-4 activity (DPP4a) is inversely associated with left ventricular function in patients with heart failure (HF) or diabetes. However, the association between DPP4a and left ventricular function in ST-segment elevation myocardial infarction (STEMI) patients has not been reported. We studied th...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-06514-3

    authors： Li JW,Chen YD,Liu YQ,Wang JD,Chen WR,Zhang YQ,Ma Q

    更新日期：2017-07-21 00:00:00

  • Analysis of Bioactive Amino Acids from Fish Hydrolysates with a New Bioinformatic Intelligent System Approach.

    abstract：:The current economics of the fish protein industry demand rapid, accurate and expressive prediction algorithms at every step of protein production especially with the challenge of global climate change. This help to predict and analyze functional and nutritional quality then consequently control food allergies in hype...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-10890-1

    authors： Elaziz MA,Hemdan AM,Hassanien A,Oliva D,Xiong S

    更新日期：2017-09-07 00:00:00

  • Mapping of multi-elements during melting and solidification using synchrotron X-rays and pixel-based spectroscopy.

    abstract：:A new synchrotron-based technique for elemental imaging that combines radiography and fluorescence spectroscopy has been developed and applied to study the spatial distribution of Ag, Zr and Mo in an Al alloy during heating and melting to 700, and then re-soldification. For the first time, multi-element distributions ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep15988

    authors： Liotti E,Lui A,Connolley T,Dolbnya IP,Sawhney KJ,Malandain A,Wilson MD,Veale MC,Seller P,Grant PS

    更新日期：2015-11-02 00:00:00

  • Ultra-Broadband Optical Gain in III-Nitride Digital Alloys.

    abstract：:A novel III-Nitride digital alloy (DA) with ultra-broadband optical gain is proposed. Numerical analysis shows a 50-period InN/GaN DA yields minibands that are densely quantized by numerous confined states. Interband transitions between the conduction and valence minibands create ultra-broadband optical gain spectra w...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21434-6

    authors： Sun W,Tan CK,Wierer JJ Jr,Tansu N

    更新日期：2018-02-15 00:00:00

  • Cardiac MLC2 kinase is localized to the Z-disc and interacts with α-actinin2.

    abstract：:Cardiac contractility is enhanced by phosphorylation of myosin light chain 2 (MLC2) by cardiac-specific MLC kinase (cMLCK), located at the neck region of myosin heavy chain. In normal mouse and human hearts, the level of phosphorylation is maintained relatively constant, at around 30-40% of total MLC2, likely by well-...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48884-w

    authors： X Cai L,Tanada Y,D Bello G,C Fleming J,F Alkassis F,Ladd T,Golde T,Koh J,Chen S,Kasahara H

    更新日期：2019-08-29 00:00:00

  • Genetic insights into underground responses to Fusarium graminearum infection in wheat.

    abstract：:The ongoing global intensification of wheat production will likely be accompanied by a rising pressure of Fusarium diseases. While utmost attention was given to Fusarium head blight (FHB) belowground plant infections of the pathogen have largely been ignored. The current knowledge about the impact of soil borne Fusari...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-31544-w

    authors： Voss-Fels KP,Qian L,Gabur I,Obermeier C,Hickey LT,Werner CR,Kontowski S,Frisch M,Friedt W,Snowdon RJ,Gottwald S

    更新日期：2018-09-03 00:00:00

  • Active layers of high-performance lead zirconate titanate at temperatures compatible with silicon nano- and microeletronic [corrected] devices.

    abstract：:Applications of ferroelectric materials in modern microelectronics will be greatly encouraged if the thermal incompatibility between inorganic ferroelectrics and semiconductor devices is overcome. Here, solution-processable layers of the most commercial ferroelectric compound--morphotrophic phase boundary lead zircona...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20143

    authors： Bretos I,Jiménez R,Tomczyk M,Rodríguez-Castellón E,Vilarinho PM,Calzada ML

    更新日期：2016-02-03 00:00:00

  • DNA methylation regulates TMEM16A/ANO1 expression through multiple CpG islands in head and neck squamous cell carcinoma.

    abstract：:ANO1 is a calcium-activated chloride channel that is frequently overexpressed in head and neck squamous cell carcinoma (HNSCC) and other cancers. While ANO1 expression negatively correlates with survival in several cancers, its epigenetic regulation is poorly understood. We analyzed HNSCC samples from TCGA and a separ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-15634-9

    authors： Finegersh A,Kulich S,Guo T,Favorov AV,Fertig EJ,Danilova LV,Gaykalova DA,Califano JA,Duvvuri U

    更新日期：2017-11-09 00:00:00