Abstract：
:The insect midgut secretes a semi-permeable, acellular peritrophic membrane (PM) that maintains intestinal structure, promotes digestion, and protects the midgut from food particles and pathogenic microorganisms. Peritrophin is an important PM protein (PMP) in the PM. Here, we identified 11 peritrophins with 1-16 chitin binding domains (CBDs) comprising 50-56 amino acid residues. Multiple CBDs in the same peritrophin clustered together, rather than by species. The CBD contained six highly conserved cysteine residues, with the key feature of amino acids between them being CX11-15CX5CX9-14CX11-12CX6-7C. Peritrophins with 2 and 4 CBDs (Bm09641 and Bm01504, respectively), and with 1, 8, and 16 CBDs (Bm11851, Bm00185, and Bm01491, respectively) were mainly expressed in the anterior midgut, and throughout the midgut, respectively. Survival rates of transgenic silkworms with Bm01504 overexpression (Bm01504-OE) and knockout (Bm01504-KO) infected with B. morinucleopolyhedrovirus (BmNPV) were significantly higher and lower, whereas expression of the key viral gene, p10, were lower and higher, respectively, compared with wild type (WT). Therefore, Bm01504-OE and Bm01504-KO transgenic silkworms were more and less resistant, respectively, to BmNPV. Bm01504 plays important roles in resisting BmNPV invasion. We provide a new perspective for studying PM function, and reveal how the silkworm midgut resists invasive exogenous pathogenic microorganisms.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zha XL,Yu XB,Zhang HY,Wang H,Huang XZ,Shen YH,Lu Cdoi
10.3390/ijms21217973subject
Has Abstractpub_date
2020-10-27 00:00:00issue
21issn
1422-0067pii
ijms21217973journal_volume
21pub_type
杂志文章abstract：:Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms11093529
更新日期：2010-09-21 00:00:00
abstract：:Advances in the use of targeted and immune therapies have revolutionized the clinical management of melanoma patients, prolonging significantly their overall and progression-free survival. However, both targeted and immune therapies suffer limitations due to genetic mutations and epigenetic modifications, which determ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21124544
更新日期：2020-06-26 00:00:00
abstract：:Bifidobacterium bifidum BGN4 is a probiotic strain that has been used as a major ingredient to produce nutraceutical products and as a dairy starter since 2000. The various bio-functional effects and potential for industrial application of B. bifidum BGN4 has been characterized and proven by in vitro (i.e., phytochemi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17091544
更新日期：2016-09-14 00:00:00
abstract：:Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates are known to tolerate superior quinolone antimicrobials compared with other antibacterial agents. Among the clones belonging to sequence type (ST) 131 by multilocus sequence typing, the involvement of the H30-Rx subclone has been reported worldwi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205162
更新日期：2019-10-18 00:00:00
abstract：:The current study examined the efficacy of royal jelly (RJ) against cadmium chloride (CdCl₂)-induced testicular dysfunction. A total of 28 Swiss male mice were allocated into four groups (n = 7), and are listed as follows: (1) the control group, who was intraperitoneally injected with physiological saline (0.9% NaCl) ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19123979
更新日期：2018-12-10 00:00:00
abstract：:With the aim of contributing to the knowledge about their potential therapeutic activity, we determined the biological activities of cyanidin and its selected O-glycosides in relation to erythrocytes (RBCs) and human dermal vascular endothelial cells (HMEC-1). Furthermore, on the basis of changes in the physical/funct...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081928
更新日期：2019-04-19 00:00:00
abstract：:Sunflower (Helianthus annuus L.) oil remains under-utilised albeit one of the major seed oils produced world-wide. Moreover, the high oleic sunflower varieties make the oil attractive for applications requiring high temperature processes and those targeting the C=C double bond functionality. Herein an overview of the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9081393
更新日期：2008-08-01 00:00:00
abstract：:Membrane traffic plays a pivotal role in virulence in the enteric protozoan parasite Entamoeba histolytica. EhRab8A small GTPase is a key regulator of membrane traffic at the endoplasmic reticulum (ER) of this protist and is involved in the transport of plasma membrane proteins. Here we identified the binding proteins...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19123831
更新日期：2018-11-30 00:00:00
abstract：:Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect CTCs mutations whi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124364
更新日期：2020-06-19 00:00:00
abstract：:Intrinsically disordered proteins (IDPs) are proteins that usually do not adopt well-defined native structures when isolated in solution under physiological conditions. Numerous IDPs have close relationships with human diseases such as tumor, Parkinson disease, Alzheimer disease, diabetes, and so on. These disease-ass...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms12053205
更新日期：2011-01-01 00:00:00
abstract：:The purpose of this study was to investigate the inhibitory activities of ethanolic extracts from Antrodia cinnamomea (EEAC) on lung cancer. Cell proliferation and cell cycle distribution were analyzed using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and flow cytometry, respectively. Wo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030791
更新日期：2018-03-09 00:00:00
abstract：:The renal cortex drives renal function. Hypoxia/reoxygenation are primary factors in ischemia-reperfusion (IR) injuries, but renal oxygenation per se is complex and awaits full elucidation. Few mathematical models address this issue: none captures cortical tissue heterogeneity. Using agent-based modeling, we develop t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246246
更新日期：2019-12-11 00:00:00
abstract：:Most pharmaceutical substances interact with several or even many molecular targets in the organism, determining the complex profiles of their biological activity. Moreover, due to biotransformation in the human body, they form one or several metabolites with different biological activity profiles. Therefore, the deve...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207492
更新日期：2020-10-11 00:00:00
abstract：:Proteinuria is a marker of incipient kidney injury in many disorders, including obesity. Previously, we demonstrated that megalin, a receptor endocytotic protein in the proximal tubule, is downregulated in obese mice, which was prevented by inhibition of dipeptidyl protease 4 (DPP4). Obesity is thought to be associate...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17050780
更新日期：2016-05-20 00:00:00
abstract：:In recent years, the overuse of antibiotics has become very serious. Many pathogenic bacteria have become resistant to them, with serious potential health consequences. Thus, it is urgent that we develop new antibiotic drugs. Antimicrobial peptides (AMPs) are important endogenous antibacterial molecules that contribut...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19102951
更新日期：2018-09-27 00:00:00
abstract：:Mass spectrometry-based metabolome profiling became the method of choice in systems biology approaches and aims to enhance biological understanding of complex biological systems. Genomics, transcriptomics, and proteomics are well established technologies and are commonly used by many scientists. In comparison, metabol...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17050632
更新日期：2016-04-27 00:00:00
abstract：:Spinocerebellar ataxia type 3 (SCA3) is a devastating neurodegenerative disease for which there is currently no cure, nor effective treatment strategy. One of nine polyglutamine disorders known to date, SCA3 is clinically heterogeneous and the main feature is progressive ataxia, which in turn affects speech, balance a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20215434
更新日期：2019-10-31 00:00:00
abstract：:The serine protease Caseinolytic protease subunit P (ClpP) plays an important role for protein homeostasis in bacteria and contributes to various developmental processes, as well as virulence. Therefore, ClpP is considered as a potential drug target in Gram-positive and Gram-negative bacteria. In this study, we utiliz...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20112686
更新日期：2019-05-31 00:00:00
abstract：:Neoplastic growth and cellular differentiation are critical hallmarks of tumor development. It is well established that cell-to-cell communication between tumor cells and "normal" surrounding cells regulates tumor differentiation and proliferation, aggressiveness, and resistance to treatment. Nevertheless, the mechani...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19051270
更新日期：2018-04-24 00:00:00
abstract：:Glial cell line-derived neurotrophic factor (GDNF) was encapsulated into liposomes in order to protect it from enzyme degradation in vivo and promote its permeability across the blood-brain barrier (BBB). In this study, GDNF conventional liposomes (GDNF-L) and GDNF target sterically stabilized liposomes (GDNF-SSL-T) w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15033612
更新日期：2014-02-27 00:00:00
abstract：:In many social anxiety disorder (SAD) patients, the efficacy of antidepressant therapy is unsatisfactory. Here, we investigated whether mice deficient for the lysosomal glycoprotein acid sphingomyelinase (ASM-/-) represent an appropriate tool to study antidepressant-resistant social fear. We also investigated whether ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218220
更新日期：2020-11-03 00:00:00
abstract：:Cells have evolved balanced systems that ensure an appropriate response to stress. The systems elicit repair responses in temporary or moderate stress but eliminate irreparable cells via apoptosis in detrimental conditions of prolonged or severe stress. The tumor suppressor p53 is a central player in these stress resp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20205179
更新日期：2019-10-18 00:00:00
abstract：:Chondrocyte apoptosis contributes to the disruption of cartilage integrity in osteoarthritis (OA). Recently, we reported that activation of volume-sensitive Cl- current (ICl,vol) mediates cell shrinkage, triggering apoptosis in rabbit articular chondrocytes. A cyclooxygenase (COX) blocker is frequently used for the tr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141019705
更新日期：2013-09-30 00:00:00
abstract：:Powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) is one of the most serious diseases in barley. The numerous barley varieties across China provide valuable genetic resources to screen the resistant germplasm and to discover the primary genes of resistance to powdery mildew. In this study, Chinese barley ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010151
更新日期：2019-12-24 00:00:00
abstract：:Brassica campestris L., a hyperaccumulator of cadmium (Cd), is considered a candidate plant for efficient phytoremediation. The hairy roots of Brassica campestris L are chosen here as a model plant system to investigate the response mechanism of Brassica campestris L. to Cd stress. High-throughput sequencing technolog...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010180
更新日期：2019-12-26 00:00:00
abstract：:A defining challenge of the 21st century is meeting the nutritional demands of the growing human population, under a scenario of limited land and water resources and under the specter of climate change. The Vavilov seed bank contains numerous landraces collected nearly a hundred years ago, and thus may contain 'geneti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21113952
更新日期：2020-05-31 00:00:00
abstract：:The combination of an antifungal agent and drug carrier with adjunctive antimicrobial properties represents novel strategy of complex therapy in pharmaceutical technology. The goal of this study was to investigate the unmodified and ion cross-linked chitosan's influence on anti-Candida activity of clotrimazole used as...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151017765
更新日期：2014-09-30 00:00:00
abstract：:The interactions of plants with environment and insects are bi-directional and dynamic. Consequently, a myriad of mechanisms has evolved to engage organisms in different types of interactions. These interactions can be mediated by allelochemicals known as volatile organic compounds (VOCs) which include volatile terpen...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21197382
更新日期：2020-10-06 00:00:00
abstract：:DEFECTIVE IN ANTHER DEHISCENCE 1 (DAD1), a phospholipase A1, utilizes galactolipids (18:3) to generate α-linolenic acid (ALA) in the initial step of jasmonic acid (JA) biosynthesis in Arabidopsis thaliana. In this study, we isolated the JcDAD1 gene, an ortholog of Arabidopsis DAD1 in Jatropha curcas, and found that it...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21238923
更新日期：2020-11-24 00:00:00
abstract：:Extracellular vesicles (EVs), including exosomes and microvesicles, are nano-to-micrometer vesicles released from nearly all cellular types. EVs comprise a mixture of bioactive molecules (e.g., mRNAs, miRNAs, lipids, and proteins) that can be transported to the targeted cells/tissues via the blood or lymph circulation...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145163
更新日期：2020-07-21 00:00:00
