Abstract:
:Hepatocellular carcinoma (HCC) is a leading cause of cancer‑related morbidity and mortality globally. Despite the remarkable improvements in comprehensive HCC treatment, the underlying mechanistic details of HCC remain elusive. We screened HCC patients for differentially expressed genes (DEGs) using the Gene Expression Omnibus (GSE113850) and The Cancer Genome Atlas (TCGA) datasets. LINC01554 expression in 40 paired samples was determined by quantitative reverse transcription polymerase chain reaction (RT‑qPCR), and its clinical significance was assessed. LINC01554 was found to have a gain‑of‑function role in HCC in vitro. Additionally, the bioinformatics analysis of the genes co‑expressed with LINC01554 was performed using the Co‑LncRNA website, and potential molecular mechanisms were investigated using the Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes resources and validated by in vitro experiments. A total of 229 DEGs were identified from the GSE113850 dataset. Among the identified DEGs, three long non‑coding RNAs (lncRNAs) (DIO3OS, LINC01554, and LINC01093) with |logFC| ≥2 and P<0.05 were screened. A total of 148 lncRNAs with |logFC| ≥1 and P<0.05 were identified from TCGA dataset. Low LINC01554 expression levels were significantly correlated with overall survival, pathological stage, hepatitis B infection, tumour size, portal vein tumour thrombus, and TNM stage. Using gain‑of‑function assays, we further showed that LINC01554 inhibited the proliferation, migration, and invasion of the HCCLM9 and SK‑Hep1 cells and promoted G0/G1 arrest, but it did not significantly affect apoptosis. Western blotting revealed that LINC01554 overexpression resulted in increased ZO‑1 and E‑cadherin expression levels, but decreased N‑cadherin and vimentin expression levels. Moreover, LINC01554 overexpression inhibited Akt, p‑Akt, β‑catenin, and p‑Gsk3β expression. Our results showed that LINC01554 repressed HCC cell invasiveness and epithelial‑to‑mesenchymal transition partly by inhibiting Wnt and PI3K‑Akt signalling in vitro. Taken together, our findings provide new insights into the molecular mechanisms underlying HCC tumourigenesis and implicate LINC01554 as a potential target for HCC therapy.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Li L,Huang K,Lu Z,Zhao H,Li H,Ye Q,Peng Gdoi
10.3892/or.2020.7779subject
Has Abstractpub_date
2020-11-01 00:00:00pages
2185-2197issue
5eissn
1021-335Xissn
1791-2431journal_volume
44pub_type
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