Abstract：
:Ochratoxin A (OTA) is a toxic metabolite that is widely distributed in food products. Herein, we proposed a new fluorescent aptasensor for OTA detection by using cascade strand displacement reaction. The binding of OTA and OTA aptamer on magnetic beads surface inhibited its hybridization with complementary DNA, and subsequently initiated the strand displacement reaction that induced amplified fluorescence signal. By tracing fluorescence response, our method demonstrated an improved detection limit of 0.63 ng/mL, a short assay time of 110 min, and a satisfactory detection specificity by using ochratoxin B, aflatoxin B1, and zearalenone as control toxins. Recovery studies were conducted by spiking OTA in real food samples, including white wine, red wine, cereal drink, coffee beverage and tea beverage, and confirmed desirable accuracy and practical applicability of our method. Therefore, our method may have a great potential use in the food quality control in the future.
journal_name
Food Chemjournal_title
Food chemistryauthors
Han B,Fang C,Sha L,Jalalah M,Al-Assiri MS,Harraz FA,Cao Ydoi
10.1016/j.foodchem.2020.127827subject
Has Abstractpub_date
2021-02-15 00:00:00pages
127827eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31689-7journal_volume
338pub_type
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