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Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method.

Abstract：

:Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities.

journal_name

Food Chem

journal_title

Food chemistry

authors

Nakamura K,Kondo K,Akiyama H,Ishigaki T,Noguchi A,Katsumata H,Takasaki K,Futo S,Sakata K,Fukuda N,Mano J,Kitta K,Tanaka H,Akashi R,Nishimaki-Mogami T

doi

10.1016/j.foodchem.2016.02.157

subject

Has Abstract

pub_date

2016-08-15 00:00:00

pages

272-9

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(16)30335-1

journal_volume

205

pub_type

杂志文章

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