Recombinant IL-2-activated NK cells mediate LAK activity against ovarian cancer.


:We investigated the ability of endogenous and recombinant interleukin-2 (IL-2)-stimulated NK cells from normal donors and ovarian cancer patients to mediate lysis of ovarian tumors, and found that peripheral blood (PB) mononuclear cells of normal donors or cancer patients did not display tumoricidal activity against ovarian cell line or fresh ovarian tumors. In addition, ovarian cancer patients displayed a defect in NK-cell activity against the highly NK-sensitive target, K-562. However, lytic activity against ovarian tumors (including cultured and primary tumors) was induced and that against K-562 was potentiated in PB of normal donors and PB and peritoneal cavity of ovarian cancer patients after enrichment of LGL on Percoll density gradient or after stimulation of effector cells with recombinant IL-2 in vitro. The IL-2-generated cytotoxic cells in PB were characterized as NK cells displaying CD16+, NKH1 (Leu-19)+, CD3- and CD5- phenotype, while those in the peritoneal cavity were predominantly CD16-, NKH1+, CD3- and CD5-. Studies on the mechanism of IL-2-dependent generation of cytotoxicity showed that such an effect was mediated via recruitment of tumor-binding cells as well as by an increase in the frequency of cytotoxic cells.


Int J Cancer


Lotzová E,Savary CA,Freedman RS,Edwards CL,Wharton JT




Has Abstract


1988-08-15 00:00:00












  • Characterization of a lung-cancer-associated auto-antigen.

    abstract::An antigen, protein X (Px), was purified from immune complexes isolated from malignant pleural effusions from patients with adenocarcinoma of the lung by EDTA treatment, PEG 8000 precipitation, protein A affinity chromatography, and Sephadex G-200 separation in the presence of 3 M NaCl. The purified antigen had a M(r)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zeng CQ,Alpert LC,Alpert E

    更新日期:1992-10-21 00:00:00

  • A pooled analysis of the outcome of prospective colonoscopic surveillance for familial colorectal cancer.

    abstract::Surveillance guidelines for the management of familial colorectal cancer (FCC), a dominant family history of colorectal cancer in which the polyposis syndromes and Lynch syndrome have been excluded, are not firmly established. The outcome of colonoscopic surveillance is studied using data from six centers. DNA mismatc...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Mesher D,Dove-Edwin I,Sasieni P,Vasen H,Bernstein I,Royer-Pokora B,Holinski-Feder E,Lalloo F,Evans DG,Forsberg A,Lindblom A,Thomas H

    更新日期:2014-02-15 00:00:00

  • A tumor-associated antigen expressed in melanoma cells with lower malignant potential.

    abstract::The antigen K-1-2, detectable by a MAb is found in nevi and melanomas. It is associated with melanoma cells of low invasive and metastatic potential as shown by immunoperoxidase studies with cell lines, biopsies and autopsies: K-1-2 occurs in melanoma cell line SK-Mel 25, but not in cell line A-375. A-375 has a higher...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Suter L,Brüggen J,Bröcker EB,Sorg C

    更新日期:1985-06-15 00:00:00

  • Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap).

    abstract::Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a "liquid biopsy". In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen lit...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Andree KC,Mentink A,Zeune LL,Terstappen LWMM,Stoecklein NH,Neves RP,Driemel C,Lampignano R,Yang L,Neubauer H,Fehm T,Fischer JC,Rossi E,Manicone M,Basso U,Marson P,Zamarchi R,Loriot Y,Lapierre V,Faugeroux V,Oulhen

    更新日期:2018-11-15 00:00:00

  • Heterogeneity of colon and rectum cancer incidence across 612 SEER counties, 2000-2014.

    abstract::Recent analyses have suggested decreases over time in colorectal cancer incidence at older ages (≥55 years) but increases at younger ages (20-54 years). Understanding the geographic heterogeneity of incidence facilitates resource allocation for potential interventions and advances our knowledge of differential etiolog...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chernyavskiy P,Kennerley VM,Jemal A,Little MP,Rosenberg PS

    更新日期:2019-04-15 00:00:00

  • Clinicopathological and prognostic significance of circulating tumor cells in patients with gastric cancer: a meta-analysis.

    abstract::The prognostic significance of circulating tumor cells (CTCs) and disseminated tumor cells (DTCs) in patients with gastric cancer (GC) is controversial. The aims of our meta-analysis are to assess its correlation with clinicopathological characteristics and prognostic significance in GC. PubMed, Embase, the Cochrane d...

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析


    authors: Huang X,Gao P,Sun J,Chen X,Song Y,Zhao J,Xu H,Wang Z

    更新日期:2015-01-01 00:00:00

  • Curcumin enhances dasatinib-induced inhibition of growth and transformation of colon cancer cells.

    abstract::Colorectal cancer is the third most common form of malignancy, behind prostate and lung cancers. Despite recent advances in medicine, mortality from colorectal cancer remains high, highlighting the need for improved therapies. Numerous studies have demonstrated increased activation of EGFR and its family members (EGFR...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nautiyal J,Banerjee S,Kanwar SS,Yu Y,Patel BB,Sarkar FH,Majumdar AP

    更新日期:2011-02-15 00:00:00

  • Telomerase activity correlates with tumor aggressiveness and reflects therapy effect in breast cancer.

    abstract::Telomerase appears to be an important factor for the control of cellular proliferation capacity and for tumorigenesis. Enzyme activity is highly increased in almost all human tumors and distinguishes them from benign lesions. Besides its diagnostic value, telomerase activity appears to be associated with tumor progres...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hoos A,Hepp HH,Kaul S,Ahlert T,Bastert G,Wallwiener D

    更新日期:1998-02-20 00:00:00

  • Cytoskeleton and paclitaxel sensitivity in breast cancer: the role of beta-tubulins.

    abstract::The antineoplastic effect of paclitaxel is mainly related to its ability to bind the beta subunit of tubulin, thus preventing tubulin chain depolarization and inducing apoptosis. The relevance of the Class I beta-tubulin characteristics have also been confirmed in the clinical setting where mutations of paclitaxel-bin...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tommasi S,Mangia A,Lacalamita R,Bellizzi A,Fedele V,Chiriatti A,Thomssen C,Kendzierski N,Latorre A,Lorusso V,Schittulli F,Zito F,Kavallaris M,Paradiso A

    更新日期:2007-05-15 00:00:00

  • Parity and hepatocellular carcinoma. A population-based study in Sweden.

    abstract::The association between parity and hepatocellular carcinoma (HCC) was studied using a data-base generated by linking 2 Swedish nation-wide registries; the Cancer Registry and the Fertility Registry. Among women born between 1925 and 1960, 133 patients with HCC recorded in the Cancer Registry between 1958 and 1984 were...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lambe M,Trichopoulos D,Hsieh CC,Ekbom A,Pavia M

    更新日期:1993-11-11 00:00:00

  • Augmentation of natural killer activity by pyran copolymer in mice.

    abstract::Treatment of older mice with pyran copolymer, a known interferon-inducer, was found to result in a rapid boosting of cell-mediated cytolytic activity against YAC-1 tumor target cells. The effector cells were characterized as being non-adherent and were presumed to be natural killer (NK) cells. Augmentation occurred in...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Santoni A,Puccetti P,Riccardi C,Herberman RB,Bonmassar E

    更新日期:1979-11-15 00:00:00

  • Treatment of Burkitt's lymphoma: randomized clinical trial of single-agent versus combination chemotherapy.

    abstract::A randomized clinical trial designed to compare the effectiveness of cytoxan (CTX) alone versus a combination consisting of CTX, vincristine (Oncovin) and methotrexate (COM) in the treatment of Burkitt's lymphoma (BL) was carried out. Nineteen patients were selected at random to receive CTX alone while 21 received COM...

    journal_title:International journal of cancer

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Olweny CL,Katongole-Mbidde E,Kaddu-Mukasa A,Atine I,Owor R,Lwanga S,Carswell W,Magrath IT

    更新日期:1976-04-15 00:00:00

  • Thymidine kinase in epithelial ovarian cancer: relationship with the other pyrimidine pathway enzymes.

    abstract::TK is a pyrimidine metabolic pathway enzyme involved in salvage DNA synthesis. What roles TK may play in epithelial ovarian cancer and the relationships between TK and the other pyrimidine pathway enzymes remain unclear. We examined TK1 gene expression by RT-PCR and related it to gene expression of TS, TP and DPD in 6...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Fujiwaki R,Hata K,Nakayama K,Moriyama M,Iwanari O,Katabuchi H,Okamura H,Sakai E,Miyazaki K

    更新日期:2002-05-20 00:00:00

  • Effect of mistimed eating patterns on breast and prostate cancer risk (MCC-Spain Study).

    abstract::Modern life involves mistimed sleeping and eating patterns that in experimental studies are associated with adverse health effects. We assessed whether timing of meals is associated with breast and prostate cancer risk taking into account lifestyle and chronotype, a characteristic correlating with preference for morni...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kogevinas M,Espinosa A,Castelló A,Gómez-Acebo I,Guevara M,Martin V,Amiano P,Alguacil J,Peiro R,Moreno V,Costas L,Fernández-Tardón G,Jimenez JJ,Marcos-Gragera R,Perez-Gomez B,Llorca J,Moreno-Iribas C,Fernández-Villa T,

    更新日期:2018-11-15 00:00:00

  • Weight change, obesity and risk of prostate cancer progression among men with clinically localized prostate cancer.

    abstract::Obesity is associated with an increased risk of fatal prostate cancer. We aimed to elucidate the importance and relevant timing of obesity and weight change for prostate cancer progression. We identified 5,158 men diagnosed with localized prostate cancer (clinical stage T1/T2) from 1986 to 2012 in the Health Professio...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Dickerman BA,Ahearn TU,Giovannucci E,Stampfer MJ,Nguyen PL,Mucci LA,Wilson KM

    更新日期:2017-09-01 00:00:00

  • Expression of mucin-associated tumor antigens is altered by cell density.

    abstract::Mucin-associated sialylated Lewis antigens are implicated in tumor cell metastasis and are used in several tests for pancreatic cancer. Despite their clinical importance, little is known about the structures of the oligosaccharides of pancreatic cancer mucins or about the regulation of their synthesis or of the synthe...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yamashita Y,Ho JJ,Cheng S,Siddiki B,Chung YS,Sowa M,Kim YS

    更新日期:1997-07-29 00:00:00

  • Blood monocytes sample MelanA/MART1 antigen for long-lasting cross-presentation to CD8+ T cells after differentiation into dendritic cells.

    abstract::Human blood monocytes are very potent to take up antigens. Like macrophages in tissue, they efficiently degrade exogenous protein and are less efficient than dendritic cells (DCs) at cross-presenting antigens to CD8+ T cells. Although it is generally accepted that DCs take up tissue antigens and then migrate to lymph ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Faure F,Jouve M,Lebhar-Peguillet I,Sadaka C,Sepulveda F,Lantz O,Berre S,Gaudin R,Sánchez-Ramón S,Amigorena S

    更新日期:2018-01-01 00:00:00

  • Cancer incidence in Ho Chi Minh City, Viet Nam, 1995-1996.

    abstract::The results from the population-based cancer registry for the city of Ho Chi Minh in 1995-1996 represent the first information on the incidence of cancer in southern Viet Nam. A total of 4,080 cancer cases in males and 4,338 in females were registered, corresponding to age-standardized incidence rates (ASRs) of 130.9 ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nguyen QM,Nguyen HC,Parkin DM

    更新日期:1998-05-18 00:00:00

  • Nutrients and gastric cancer risk. A population-based case-control study in Sweden.

    abstract::A population-based case-control study of gastric cancer was conducted in areas with contrasting incidence rates in Sweden. Face-to-face interviews were carried out with 338 (74.1%) of all eligible cases and 679 (77.3%) of the selected controls. Consumption of selected nutrients during adolescence and 20 years prior to...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hansson LE,Nyrén O,Bergström R,Wolk A,Lindgren A,Baron J,Adami HO

    更新日期:1994-06-01 00:00:00

  • Lactation and the risk of breast cancer in an Italian population.

    abstract::The relation between breast feeding and breast cancer was investigated in a multicentric case-control study conducted in Italy on 2,167 parous women with histologically confirmed breast cancer, diagnosed within 1 year, and 2,208 parous control women admitted to hospitals in the same catchment areas of cases for acute,...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Negri E,Braga C,La Vecchia C,Levi F,Talamini R,Franceschi S

    更新日期:1996-07-17 00:00:00

  • Tumor-specific exon creation of the HELLS/SMARCA6 gene in non-small cell lung cancer.

    abstract::To identify tumor-suppressor genes on chromosome 10 in non-small cell lung cancers, we isolated 10 types of splicing variant of the HELLS/SMARCA6 gene transcripts. HELLS/SMARCA6 is a novel member of SNF2 family, which is implicated in cellular functions like chromatin remodeling. Variant 1 was an alternatively spliced...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yano M,Ouchida M,Shigematsu H,Tanaka N,Ichimura K,Kobayashi K,Inaki Y,Toyooka S,Tsukuda K,Shimizu N,Shimizu K

    更新日期:2004-10-20 00:00:00

  • Enzyme-linked immunosorbent assay (ELISA) for IgA and IgG antibodies to Epstein-Barr-virus ribonucleotide reductase in patients with nasopharyngeal carcinoma.

    abstract::661 bp coding for the carboxyl end of the large sub-unit of EBV ribonucleotide reductase was cloned into the pMal plasmid vector. Purified recombinant protein was tested in IgG and IgA ELISAs. For the IgG assay, 81 out of 100 NPC patients tested positive, whereas for the IgA assay, 60 tested positive. Among 100 normal...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Fones-Tan A,Chan SH,Tsao SY,Gan LH,Tan WH,Li B,Khong PW,Gan YY

    更新日期:1994-12-15 00:00:00

  • LiSa-2, a novel human liposarcoma cell line with a high capacity for terminal adipose differentiation.

    abstract::LiSa-2 is a stable cell line derived from a poorly differentiated, pleomorphic liposarcoma. In serum-containing medium, LiSa-2 cells are fibroblastoid and rapidly dividing. In a serum-free, chemically defined culture medium containing physiological concentrations of insulin, triiodothyronine and cortisol, LiSa-2 cells...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wabitsch M,Brüderlein S,Melzner I,Braun M,Mechtersheimer G,Möller P

    更新日期:2000-12-15 00:00:00

  • GPER-induced signaling is essential for the survival of breast cancer stem cells.

    abstract::G protein-coupled estrogen receptor-1 (GPER), a member of the G protein-coupled receptor (GPCR) superfamily, mediates estrogen-induced proliferation of normal and malignant breast epithelial cells. However, its role in breast cancer stem cells (BCSCs) remains unclear. Here we showed greater expression of GPER in BCSCs...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chan YT,Lai AC,Lin RJ,Wang YH,Wang YT,Chang WW,Wu HY,Lin YJ,Chang WY,Wu JC,Yu JC,Chen YJ,Yu AL

    更新日期:2020-03-15 00:00:00

  • Conundrums in screening for cancer.

    abstract::Screening for cancer has to be carefully organized for maximum effectiveness, and introduced in full understanding of the natural history of the disease. There are major potential harms as well as benefits from screening. The current state of art for breast, cervix and prostate cancer screening is reviewed, only for b...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Miller AB

    更新日期:2010-03-01 00:00:00

  • Cell proliferation in 3,800 node-negative breast cancers: consistency over time of biological and clinical information provided by 3H-thymidine labelling index.

    abstract::For breast cancer, many prognostic markers that initially appeared promising have failed to maintain their clinical predictive value. Few reports have analyzed the consistency over time of biological and clinical information provided by biomarkers. Tumour cell proliferation has acquired relevance as an indicator of pr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Silvestrini R,Daidone MG,Luisi A,Mastore M,Leutner M,Salvadori B

    更新日期:1997-02-20 00:00:00

  • Increasing risk of uterine cervical cancer among young Japanese women: Comparison of incidence trends in Japan, South Korea and Japanese-Americans between 1985 and 2012.

    abstract::In Japan, cervical cancer incidence has increased since the late 1990s especially among young women, despite a decreasing trend in most developed countries. Here, we examined age, period and birth cohort trends in cervical cancer incidence rates from 1985 to 2012. Incidence rates were ascertained using three populatio...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Utada M,Chernyavskiy P,Lee WJ,Franceschi S,Sauvaget C,de Gonzalez AB,Withrow DR

    更新日期:2019-05-01 00:00:00

  • High frequency of inactivation of the imprinted H19 gene in "sporadic" hepatoblastoma.

    abstract::Embryonal tumors such as Wilms' tumor (WT), embryonal rhabdomyosarcoma (eRMS) and hepatoblastoma have been thought to have a common pathogenetic mechanism. H19 was found to be inactivated in WT and eRMS either by loss of heterozygosity or by hypermethylation of the maternal allele. We show here that the expression of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Fukuzawa R,Umezawa A,Ochi K,Urano F,Ikeda H,Hata J

    更新日期:1999-08-12 00:00:00

  • Role of activated c-H-ras oncogene in the induction and progression of immortal liver epithelial cell lines derived from normal C3H mice.

    abstract::The nature of 15 immortal mouse liver epithelial cell (MLEC) lines established from normal C3H mice was investigated specifically in terms of ras oncogene activation. Neither transforming activity nor point mutation within codon 61 of c-H-ras could be demonstrated in any of the cell lines by DNA transfection in a NIH/...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lee GH,Sakai R,Nagao M,Kitagawa T

    更新日期:1991-01-02 00:00:00

  • Characterization of cell lines established from human hepatocellular carcinoma.

    abstract::We characterized 8 human hepatocellular-carcinoma cell lines established from the primary tumors of Korean patients. All lines showed substrate adherence and one line from anaplastic tumor also grew as floating aggregates. Most cultured cells maintained many morphological characteristics of the original tumors from wh...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Park JG,Lee JH,Kang MS,Park KJ,Jeon YM,Lee HJ,Kwon HS,Park HS,Yeo KS,Lee KU

    更新日期:1995-07-28 00:00:00