Characterization, spectroscopic and crystallographic analyses of β-lactoglobulin and docosahexaenoic acid nanocomplexes.

Abstract:

:In this work, we investigated the interaction of docosahexaenoic acid (DHA) with β-lactoglobulin (β-Lg) using spectroscopic and crystallographic methods. The fluorescence results showed that DHA formed complexes with β-Lg with a binding constant of 4.13 × 104 M-1. The secondary structure of β-Lg was not significantly (p > 0.05) changed after binding with DHA. Dynamic light scattering showed the particle size of β-Lg-DHA complexes was about 5 nm, the same as that of β-Lg alone. The turbidity of DHA in aqueous solution decreased after binding with β-Lg. The crystallographic results showed that DHA was bound at one site in the calyx of β-Lg and that the aliphatic chain was hidden inside the hydrophobic β-barrel while the carboxyl group was located at the calyx entrance. These findings indicate that β-Lg can act as an effective nanocarrier for DHA.

journal_name

Food Chem

journal_title

Food chemistry

authors

Chen X,Liu J,Jiang L,Zhang Y,Ren F,Zhang H

doi

10.1016/j.foodchem.2020.127145

subject

Has Abstract

pub_date

2020-11-15 00:00:00

pages

127145

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(20)31007-4

journal_volume

330

pub_type

杂志文章
  • An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.

    abstract::Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produ...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2018.04.085

    authors: Huang Q,Bu T,Zhang W,Yan L,Zhang M,Yang Q,Huang L,Yang B,Hu N,Suo Y,Wang J,Zhang D

    更新日期:2018-10-01 00:00:00

  • Influence of low O2 and high CO2 environment on changes in metabolite concentrations in harvested vegetable soybeans.

    abstract::Influence of atmosphere and storage period on the physicochemical and biological properties of harvested vegetable soybeans stored for 10 d at 25 °C was investigated. Storing vegetable soybeans under modified atmosphere (low O2 and high CO2), was more effective in maintaining its green color and mass than storing them...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2020.126380

    authors: Makino Y,Nishizaka A,Yoshimura M,Sotome I,Kawai K,Akihiro T

    更新日期:2020-07-01 00:00:00

  • Determination of heterocyclic amines in braised sauce beef.

    abstract::A simplified and improved analysis method to determine the content of heterocyclic amines (HAs) was developed. Samples were extracted with ethyl acetate, and macrosubstances were removed by centrifugation. The eluent used consisted of methanol/ammonia water (9:1, v/v), and the eluates were directly mixed for further H...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2013.05.010

    authors: Yao Y,Peng ZQ,Wan KH,Shao B,Shi JM,Zhang YW,Wang FL,Hui T

    更新日期:2013-12-01 00:00:00

  • Application of the solvent extraction technique to investigation of the anti-inflammatory activity of adlay bran.

    abstract::The current study utilised a bioassay-directed chemical analysis scheme to screen the anti-inflammatory activity of fractions and compounds from adlay bran (AB). Liquid-liquid extraction couple with liquid chromatography-mass spectrometry (LC-MS) was applied to the isolation, analysis and identification of active comp...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2013.08.071

    authors: Huang DW,Wu CH,Shih CK,Liu CY,Shih PH,Shieh TM,Lin CI,Chiang W,Hsia SM

    更新日期:2014-02-15 00:00:00

  • Feasibility and application of an HPLC/UVD to determine dinotefuran and its shorter wavelength metabolites residues in melon with tandem mass confirmation.

    abstract::A new analytical method was developed for dinotefuran and its metabolites, MNG, UF, and DN, in melon using high-performance liquid chromatography (HPLC) coupled with an ultraviolet detector (UVD). Due to shorter wavelength, lower sensitivity to UV detection, and high water miscibility of some metabolites, QuEChERs ace...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2012.08.064

    authors: Rahman MM,Park JH,Abd El-Aty AM,Choi JH,Yang A,Park KH,Nashir Uddin Al Mahmud M,Im GJ,Shim JH

    更新日期:2013-01-15 00:00:00

  • Supramolecular solvents combined with layered double hydroxide-coated magnetic nanoparticles for extraction of bisphenols and 4-tert-octylphenol from fruit juices.

    abstract::A magnesium-aluminum layered double hydroxides coated on magnetic nanoparticles (Fe3O4@MgAl-LDHs NPs) combined with the supramolecular solvents (SUPRASs) as the magnetic supramolecular fluids were successfully applied to the extraction and determination of four phenolic compounds (Bisphenol-A (BPA), bisphenol-AF (BPAF...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2017.06.063

    authors: Yang D,Li X,Meng D,Wang M,Yang Y

    更新日期:2017-12-15 00:00:00

  • Rising CO2 concentration altered wheat grain proteome and flour rheological characteristics.

    abstract::Wheat cv. H45 was grown under ambient CO2 concentration and Free Air CO2 Enrichment (FACE; e[CO2], ∼550 μmol CO2 mol(-1)). The effect of FACE on wheat grain proteome and associated changes in the flour rheological properties was investigated. A comparative proteomic analysis was performed using 2-D-DIGE followed by MA...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2014.07.044

    authors: Fernando N,Panozzo J,Tausz M,Norton R,Fitzgerald G,Khan A,Seneweera S

    更新日期:2015-03-01 00:00:00

  • Can artisanal "Coalho" cheese from Northeastern Brazil be used as a functional food?

    abstract::Brazilian artisanal "Coalho" cheeses from six Northeast towns were investigated as a functional food based on their peptide profiles and antioxidant, zinc-binding and antimicrobial activities. The peptides (WSP) from "Coalho" cheese showed high antioxidant activity, the best value of TEAC being 2223±10.10μM, which mea...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2012.06.058

    authors: Silva RA,Lima MS,Viana JB,Bezerra VS,Pimentel MC,Porto AL,Cavalcanti MT,Lima Filho JL

    更新日期:2012-12-01 00:00:00

  • Antifungal effects of thymol and salicylic acid on cell membrane and mitochondria of Rhizopus stolonifer and their application in postharvest preservation of tomatoes.

    abstract::This study investigated effects of the simultaneous application of thymol and salicylic acid (SIMTSA) on the target sites of Rhizopus stolonifer, as well as the defenceenzymes of postharvest tomato, when applied as edible coating. SIMTSA induced the changes of ultrastructure and membrane integrity of R. stolonifer. Wh...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2019.01.099

    authors: Kong J,Zhang Y,Ju J,Xie Y,Guo Y,Cheng Y,Qian H,Quek SY,Yao W

    更新日期:2019-07-01 00:00:00

  • Characteristics and storage stability of nanoliposomes loaded with shrimp oil as affected by ultrasonication and microfluidization.

    abstract::Shrimp oil, a rich source of n-3 fatty acids and astaxanthin, was encapsulated in nanoliposomes, prepared using ultrasonication (US) and microfluidization (MF) methods, to prevent oxidation during storage. Nanoliposomes prepared by US and MF were characterized based on particle size, structure and stability. The parti...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2019.125916

    authors: Gulzar S,Benjakul S

    更新日期:2020-04-25 00:00:00

  • Effects of hydroxycinnamic acids on the reduction of furan and α-dicarbonyl compounds.

    abstract::The effects of hydroxycinnamic acids such as cinnamic acid (CNA), p-coumaric acid(CMA), caffeic acid (CFA), and chlorogenic acid (CGA) on the reduction of furan in canned-coffee model systems (CCMS) containing α-dicarbonyls [glyoxal (GO) or methylglyoxal (MGO)] were investigated. The concentration of furan in CCMS con...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2019.126085

    authors: Lee SM,Zheng LW,Jung Y,Hwang GS,Kim YS

    更新日期:2020-05-15 00:00:00

  • Quantifying fenobucarb residue levels in beef muscles using liquid chromatography-tandem mass spectrometry and QuEChERS sample preparation.

    abstract::This paper describes a comparison of the properties of the three versions of the QuEChERS method (quick, easy, cheap, effective, rugged and safe) - the original (unbuffered), acetate-buffered, and citrate-buffered methods - for the determination of fenobucarb residues in beef muscles via liquid chromatography-electros...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2012.12.029

    authors: Park KH,Choi JH,Abd El-Aty AM,Musfiqur Rahman M,Jang J,Ko AY,Kwon KS,Park HR,Kim HS,Shim JH

    更新日期:2013-06-15 00:00:00

  • Impact of quercetin and fish oil encapsulation on bilayer membrane and oxidation stability of liposomes.

    abstract::Unsaturated soy phosphatidyl choline (PC) liposomes were systematically analyzed for chemical and physical stability and for influence on membrane fluidity, when quercetin and fish oil were encapsulated. The physical stability of liposomes was lowered with loading, which is mainly due to fish oil leakage. While fish o...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2015.03.121

    authors: Frenzel M,Steffen-Heins A

    更新日期:2015-10-15 00:00:00

  • A comprehensive investigation of guaiacyl-pyranoanthocyanin synthesis by one-/two-dimensional NMR and UPLC-DAD-ESI-MS(n).

    abstract::In red and rosé wines, the grape anthocyanins are progressively converted to more stable pigments, including phenylpyranoanthocyanins. One-/two-dimensional NMR and UPLC-DAD-ESI-MS(n) measurements were used to monitor the synthesis of guaiacylpyranomalvidin 3-O-glucoside from malvidin 3-O-glucoside and vinylguaiacol in...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2015.12.089

    authors: Vallverdú-Queralt A,Meudec E,Ferreira-Lima N,Sommerer N,Dangles O,Cheynier V,Le Guernevé C

    更新日期:2016-05-15 00:00:00

  • Green and efficient removal of cadmium from rice flour using natural deep eutectic solvents.

    abstract::Natural deep eutectic solvents (NADESs) constitute a novel class of biodegradable and inexpensive solvents. In this study, twenty choline chloride- and glycerol-based NADESs were prepared and investigated as washing agents in the removal of cadmium (Cd) from rice flour for the first time. Choline chloride-based NADESs...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2017.10.060

    authors: Huang Y,Feng F,Chen ZG,Wu T,Wang ZH

    更新日期:2018-04-01 00:00:00

  • The impact of whey protein preheating on the properties of emulsion gel bead.

    abstract::Thermal treatment effect (70 or 80 °C for 5 or 30 min) was evaluated on functional properties of whey protein isolate (WPI) dispersions used for the development of novel vitamin A delivery systems based on emulsion gel beads. This process combines an (O/W) emulsion diluted by a polysaccharide solution and a cold-set g...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2013.11.071

    authors: Ruffin E,Schmit T,Lafitte G,Dollat JM,Chambin O

    更新日期:2014-05-15 00:00:00

  • Purification and characterization of type II collagen from chick sternal cartilage.

    abstract::Type II collagen was purified from sternal cartilage of the chick using a combination of pepsin digestion, NaCl precipitation and DEAE-sepharose CL 6B ion exchange chromatography. Pepsin-solubilized type II collagen of higher stability can be obtained with the extraction time of 32h, 0.5% pepsin concentration at 20°C....

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2007.09.022

    authors: Cao H,Xu SY

    更新日期:2008-05-15 00:00:00

  • Gastric digestion of cow and goat milk: Peptides derived from simulated conditions of infant digestion.

    abstract::Infant formula products are predominantly manufactured using cow milk protein; goat milk also provides a suitable protein source. In this study, we directly compared cow and goat milk protein digestion using pH and enzyme conditions to simulate infant gastric conditions. Generated peptides, identified using liquid chr...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2018.10.065

    authors: Hodgkinson AJ,Wallace OAM,Smolenski G,Prosser CG

    更新日期:2019-03-15 00:00:00

  • Production of hydrogen peroxide in formulated beverages is associated with the presence of ascorbic acid combined with selected redox-active functional ingredients.

    abstract::Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that mediates essential signaling in vivo but may cause irreversible tissue damage under dysregulated or acute exposure conditions. Beverages containing redox-active compounds might produce H2O2 during shelf storage and potentially be consumed. Concentrations...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2020.127947

    authors: Bopitiya D,Christensen D,Martin M,Zhang J,Bennett LE

    更新日期:2021-02-15 00:00:00

  • The phytochemical variability of fatty acids in basil seeds (Ocimum basilicum L.) affected by genotype and geographical differences.

    abstract::Basil (Ocimum basilicum) from the Lamiaceae family is among the most important medicinal plants, and its seed fatty acid (FA) composition and quantity affects its nutritional and health values. It was hypothesized basil species and geographical properties significantly affect seed FA composition and quantity, which ha...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2018.10.027

    authors: Mostafavi S,Asadi-Gharneh HA,Miransari M

    更新日期:2019-03-15 00:00:00

  • Cocoa and chocolate are sources of vitamin D2.

    abstract::Cocoa beans are susceptible to fungal contamination and often contain substantial amounts of ergosterol, the precursor to vitamin D2. We hypothesized that sun-drying the fermented cocoa beans might lead to the conversion of ergosterol to vitamin D2. We quantified vitamin D in cocoa and cocoa-based foods by liquid chro...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2018.06.098

    authors: Kühn J,Schröter A,Hartmann BM,Stangl GI

    更新日期:2018-12-15 00:00:00

  • Aliphatic organic acids as promising authenticity markers of bracatinga honeydew honey.

    abstract::This study aimed to differentiate bracatinga (Mimosa scabrella Bentham) honeydew honey (BHH) from blossom honeys and BHH intentionally adulterated, all of them from three harvests, associating data of aliphatic organic acids (AOA) determined by capillary electrophoresis and chemometric analyses. The profile and concen...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2020.128449

    authors: Seraglio SKT,Bergamo G,Brugnerotto P,Gonzaga LV,Fett R,Costa ACO

    更新日期:2021-05-01 00:00:00

  • Cross priming amplification with nucleic acid test strip analysis of mutton in meat mixtures.

    abstract::A simple, sensitive, accurate and affordable rapid detection of meat species authentication is urgently needed in food industry. In this study, a cross priming amplification (CPA) combining nucleic acid test strip (CPA-Strip) assay for rapid detection of mutton from meat mixture were developed and its feasibility was ...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2017.08.107

    authors: Feng T,Li S,Wang S,Pan J

    更新日期:2018-04-15 00:00:00

  • Study of composition of espresso coffee prepared from various roast degrees of Coffea arabica L. coffee beans.

    abstract::Espresso coffee samples prepared at various roasting degrees defined according to its basic conventional classification (light, medium, medium-dark and dark roasted) were analyzed by ultra-performance liquid chromatography/tandem mass spectrometry. Obtained raw data were processed using multivariate statistical analys...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2015.12.080

    authors: Kučera L,Papoušek R,Kurka O,Barták P,Bednář P

    更新日期:2016-05-15 00:00:00

  • Magnetic microsphere sorbent on CaCO3 templates: Simple synthesis and efficient extraction of trace carbamate pesticides in fresh produce.

    abstract::Polypyrrole magnetic microspheres were synthesized and used to extract carbaryl, carbofuran, and methomyl before analysis by a high-performance liquid chromatography with diode array detection. Under optimal conditions, four times the preconcentration was achieved with the use of only 1.2 mL of sample. Good linearity ...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2020.128336

    authors: Changsan T,Wannapob R,Kaewpet M,Shearman K,Wattanasin P,Cheung Mak W,Kanatharana P,Thavarungkul P,Thammakhet-Buranachai C

    更新日期:2021-04-16 00:00:00

  • Vitamins A and E content of commercial infant foods in the UK: A cause for concern?

    abstract::According to the European Food Safety Authority, currently, there are no reliable data or robust guidelines available in relation to the micronutrient composition of infant foods. This study evaluated the intake of vitamins A and E of infants from 'ready-to-feed' foods and formulas. Normal phase high performance liqui...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2016.04.014

    authors: Loughrill E,Govinden P,Zand N

    更新日期:2016-11-01 00:00:00

  • Determination of starting materials, intermediates, and subsidiary colors in the color additive Food Red No. 106 (Sulforhodamine B) using high-performance liquid chromatography.

    abstract::The main subsidiary color of structure in Food Red No. 106 (R106) was identified to be a desethyl derivative (R106-SubA). High-performance liquid chromatography (HPLC) was performed for the quantitative determination of benzaldehyde-2,4-disulfonic acid, N,N-diethyl-m-aminophenol, leuco acid, pyrone acid, R106-SubA, et...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2017.05.084

    authors: Tatebe C,Ohtsuki T,Fujita T,Nishiyama K,Itoh S,Sugimoto N,Kubota H,Tada A,Sato K,Akiyama H

    更新日期:2017-12-15 00:00:00

  • Impact of blanching on polyphenol stability and antioxidant capacity of innovative coriander (Coriandrum sativum L.) pastes.

    abstract::Fresh coriander leaves were steam- and water-blanched at 100 °C and at 90 and 100 °C, respectively, for 1-10 min, and subsequently comminuted to form a paste. Pasty products obtained from coriander fruits were processed after water-blanching applying the same time-temperature regimes. Among the 11 phenolics characteri...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2013.02.077

    authors: Kaiser A,Kammerer DR,Carle R

    更新日期:2013-09-01 00:00:00

  • Performances of different protocols for exocellular polysaccharides extraction from milk acid gels: Application to yogurt.

    abstract::Dextran or xanthan were used as model exocellular polysaccharides (EPS) to compare the extraction efficiency of EPS from skim milk acid gels using three different protocols. Extraction yields, residual protein concentrations and the macromolecular properties of extracted EPS were determined. For both model EPS, the hi...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2017.06.121

    authors: Nguyen AT,Nigen M,Jimenez L,Ait-Abderrahim H,Marchesseau S,Picart-Palmade L

    更新日期:2018-01-15 00:00:00

  • Concentrations of PCDD/Fs, dioxin-like PCBs, PBDEs, and hexachlorobenzene in fat samples from cattle of different ages and gender in Korea.

    abstract::Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), dioxin-like polychlorinated biphenyls (DL-PCBs), polybrominated diphenyl ethers (PBDEs), and hexachlorobenzene (HCB) concentrations were determined in abdominal fat samples from 30 cattle. The relationships between chemicals, age, and gender were investiga...

    journal_title:Food chemistry

    pub_type: 杂志文章

    doi:10.1016/j.foodchem.2012.11.072

    authors: Kim M,Kim DG,Bong YH,Jang JH,Son SW

    更新日期:2013-06-01 00:00:00