当前位置： SCI文献检索 > INTERNATIONAL IMMUNOPHARMACOLOGY期刊下所有文献 > Design, expression, and evaluation of novel multiepitope chimeric antigen of Wuchereria bancrofti for the diagnosis of lymphatic filariasis - A structure-based strategy.

Design, expression, and evaluation of novel multiepitope chimeric antigen of Wuchereria bancrofti for the diagnosis of lymphatic filariasis - A structure-based strategy.

Abstract：

:The Global Program for Elimination Lymphatic Filariasis (GPELF) is in an advanced stage and requires tools for diagnosing infection, assessing transmission and certification. This study was aimed at developing an antibody-based assay using a chiemric antigen containing multi-B-cell epitopes from antigens highly expressed in different stages of Wuchereria bancrofti to detect LF infection and its transmission. The antigen was express cloned and two indirect ELISA based (IgG1 & IgG4 based) antibody assays were developed using the recombinant antigen. The chimeric antigen displayed 1 and 3-fold reactivity with IgG1 and IgG4 antibodies, respectively in microfilaraial (mf) positive sera when compared to that in sera samples of Non-endemic normal sera (NEN) (O.D, 0.13 ± 0.20 and 0.18 ± 0.07), thus differentiating infected from uninfected individuals. In IgG1 and IgG4 antibody assays, the multiepitope antigen also showed reactivity (O.D, 0.27 ± 0.18 and 0.16 ± 0.03) in a small proportion (18 and 30, respectively out of 156) endemic normal individuals and in IgG1 antibody in a few (4) chronic patients (CP). The antigen did not react with IgG1 or IgG4 antibodies in the sera samples of malaria, scrub typhus, dengue, hookworm, and roundworm helminth cases (0.139 ± 0.018, 0.144 ± 0.007 0.17804 ± 0.007 and 0.162 ± 0.006), thus showing its high specificity. The sensitivity (%) and specificity (%) of the multi-epitope antigen-based IgG1 and IgG4 antibody assays are 100, 98.1 and 100, 99.52, respectively. Thus, the recombinant multiepitope antigen appears to have good potential in detecting active LF infection and in assessing its transmission in endemic communities.

journal_name

Int Immunopharmacol

journal_title

International immunopharmacology

authors

Yasin N,Sugerappa Laxmanappa H,Muddapur UM,Cheruvathur J,Uday Prakash SM,Venkataramaiah Thulasiram H

doi

10.1016/j.intimp.2020.106431

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

106431

eissn

1567-5769

issn

1878-1705

pii

S1567-5769(20)30263-0

journal_volume

83

pub_type

杂志文章

相关文献

INTERNATIONAL IMMUNOPHARMACOLOGY文献大全
  • Immunogenic cross-reaction among outer membrane proteins of Gram-negative bacteria.

    abstract：:In the present study, antigenic cross-reactivity of OMPs was investigated in several species of bacterial pathogens. Heterogeneous mouse or fish antisera were used to ascertain OMPs with cross-reactivity and cluster analysis was performed to analyze the distribution of cross-antigenic OMPs in diverse bacterial strains...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2005.02.008

    authors： Xu C,Wang S,Zhaoxia Z,Peng X

    更新日期：2005-07-01 00:00:00

  • Assembly, activation, and physiologic influence of the plasma kallikrein/kinin system.

    abstract：:The plasma kallikrein/kinin system that consists of the proteins factor XII, prekallikrein, and high molecular weight kininogen was first recognized as a surface-activated coagulation system arising when blood or plasma interacts with artificial surfaces. Although surface-activated contact activation occurs in vivo wh...

    journal_title：International immunopharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.intimp.2007.08.022

    authors： Schmaier AH

    更新日期：2008-02-01 00:00:00

  • Mogroside IVE attenuates experimental liver fibrosis in mice and inhibits HSC activation through downregulating TLR4-mediated pathways.

    abstract：:Liver fibrosis has been emphasized as a serious threat to human health. There is currently no effective clinical drug treatment. Although mogrosides (MGs) have extensive pharmacological effects with minimal toxicity, their effects on liver function, inflammation, matrix metalloproteinases and hepatic stellate cell (HS...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2017.12.023

    authors： Cao F,Zhang Y,Li W,Shimizu K,Xie H,Zhang C

    更新日期：2018-02-01 00:00:00

  • Anti-neuroinflammatory effect of aurantiamide acetate from the marine fungus Aspergillus sp. SF-5921: inhibition of NF-κB and MAPK pathways in lipopolysaccharide-induced mouse BV2 microglial cells.

    abstract：:In the course of a search for anti-neuroinflammatory metabolites from marine fungi, aurantiamide acetate (1) was isolated from marine-derived Aspergillus sp. as an anti-neuroinflammatory component. Compound 1 dose-dependently inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in BV2 microglial c...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2014.10.006

    authors： Yoon CS,Kim DC,Lee DS,Kim KS,Ko W,Sohn JH,Yim JH,Kim YC,Oh H

    更新日期：2014-12-01 00:00:00

  • Butyrate alleviates inflammatory response and NF-κB activation in human degenerated intervertebral disc tissues.

    abstract：:Butyrate has multiple protective effects in inflammation-related intestinal diseases. Previous studies have found that butyrate could inhibit inflammation in rheumatoid arthritis. Inflammation is a pivotal inducement in the degeneration progress of the intervertebral disc. The anti-inflammatory treatment has an appare...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2019.106004

    authors： Jia J,Nie L,Liu Y

    更新日期：2020-01-01 00:00:00

  • Nicotine modulation of apoptosis in human coronary artery endothelial cells.

    abstract：:It has been recently reported that nicotine, the addictive component of tobacco, is an important modulator at the level of immune cell apoptosis or programmed cell death. Apoptosis is a process that helps maintain the homeostasis of the vascular endothelium and vascular smooth muscle cells, and alteration of the apopt...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/s1567-5769(02)00059-0

    authors： Hakki A,Friedman H,Pross S

    更新日期：2002-09-01 00:00:00

  • Berberine suppresses IL-33-induced inflammatory responses in mast cells by inactivating NF-κB and p38 signaling.

    abstract：:Berberine (BBR) possesses many pharmacological characteristics including anti-inflammation, anti-allergy, anti-angiogenesis and anti-tumor. However, the effects and mechanisms of BBR on IL-33-induced mast cell inflammatory responses are kept unknown. To investigate these, rat peritoneal mast cells (RPMCs) were isolate...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2018.11.009

    authors： Li W,Yin N,Tao W,Wang Q,Fan H,Wang Z

    更新日期：2019-01-01 00:00:00

  • The impact of a new immunomodulator oxo-quinoline-3-carboxamide on the progression of experimental lupus.

    abstract：:Autoimmune, lupus-prone MRL lpr/lpr mice were treated orally with oxo-quinoline-3-carboxamide (ABR-25757), a newly developed immunomodulator. Treatment was initiated in one set of experiment at the age of 10 weeks, before the onset of clinically apparent disease, and in another set at 15 weeks, after the development o...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2004.07.009

    authors： Carlsten H,Jonsson C,Bokarewa M,Svensson L,Tarkowski A

    更新日期：2004-11-01 00:00:00

  • Efficacy and safety of dendritic cell vaccines for patients with glioblastoma: A meta-analysis of randomized controlled trials.

    abstract：BACKGROUND:Dendritic cell (DC)-based vaccination has been suggested to be promising for glioblastoma. However, the evidence in randomized controlled trials (RCTs) is inconsistent. We aimed to systematically evaluate the efficacy and safety of DC vaccine for glioblastoma via a meta-analysis of RCTs. METHODS:Related ran...

    journal_title：International immunopharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.intimp.2020.106336

    authors： Lv L,Huang J,Xi H,Zhou X

    更新日期：2020-06-01 00:00:00

  • Dimethyl fumarate and curcumin attenuate hepatic ischemia/reperfusion injury via Nrf2/HO-1 activation and anti-inflammatory properties.

    abstract：BACKGROUND:Hepatic ischemia/reperfusion (I/R) injury occurs in different clinical settings as hepatic transplantation, and different types of shock. I/R injury is the main cause of hepatic damage and failure due to the production of reactive oxygen species (ROS) and inflammatory cytokines. Dimethyl fumarate (DMF), an i...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2019.106131

    authors： Ibrahim SG,El-Emam SZ,Mohamed EA,Abd Ellah MF

    更新日期：2020-03-01 00:00:00

  • Identification and verification immune-related regulatory network in acne.

    abstract：:Acne is a common inflammatory skin disease with the dysregulation of innate and adaptive immunity. However, the underlying mechanism of acne has not been completely elucidated. In this study, we identified gene signatures and the immune-related regulatory network in acne using integrated bioinformatics methods. Here, ...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2020.107083

    authors： Xin Y,Zhang S,Deng Z,Zeng D,Li J,Zhang Y

    更新日期：2020-12-01 00:00:00

  • Evaluation of immunosuppression induced by metronidazole in Balb/c mice and human peripheral blood lymphocytes.

    abstract：:The immunomodulatory effect of metronidazole (MTZ), a nitroimidazole drug used as an antiprotozoal and antibacterial agent, was investigated using Balb/c mice and human peripheral blood lymphocytes. For in vivo studies, mice were divided into six groups, six animals per group, group I received vehicle alone while the ...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2007.10.018

    authors： Fararjeh M,Mohammad MK,Bustanji Y,Alkhatib H,Abdalla S

    更新日期：2008-02-01 00:00:00

  • BCG-infected adherent mononuclear cells release cytokines that regulate group 1 CD1 molecule expression.

    abstract：:Increasing evidence is now available showing that CD1-restricted T cell responses against non-peptide mycobacterial antigens could play a role in the immune resistance against tuberculosis. BCG, widely used in anti-tubercular vaccination, shares various constituents with Mycobacterium tuberculosis, but does not provid...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2006.11.003

    authors： Prete SP,Giuliani A,D'Atri S,Graziani G,Balduzzi A,Oggioni MR,Iona E,Girolomoni G,Bonmassar L,Romani L,Franzese O

    更新日期：2007-03-01 00:00:00

  • Receptors for chemotactic formyl peptides as pharmacological targets.

    abstract：:Leukocytes accumulate at sites of inflammation and immunological reaction in response to locally existing chemotactic mediators. N-formyl peptides, such as fMet-Leu-Phe (fMLF), are some of the first identified and most potent chemoattractants for phagocytic leukocytes. In addition to the bacterial peptide fMLF and the...

    journal_title：International immunopharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/s1567-5769(01)00150-3

    authors： Le Y,Yang Y,Cui Y,Yazawa H,Gong W,Qiu C,Wang JM

    更新日期：2002-01-01 00:00:00

  • Antitumor and immunomodulatory properties of artemether and its ability to reduce CD4+ CD25+ FoxP3+ T reg cells in vivo.

    abstract：BACKGROUND:Development of agents that specifically kill cancer cells and simultaneously elicit antitumor immune response is a step forward in cancer therapy. In the present study, we investigated whether the administration of artemether contributes to the augmentation of antitumor immunity and the regression of tumor t...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2011.07.008

    authors： Farsam V,Hassan ZM,Zavaran-Hosseini A,Noori S,Mahdavi M,Ranjbar M

    更新日期：2011-11-01 00:00:00

  • Bone status of adult female butyrylcholinesterase gene-deficient mice.

    abstract：:Butyrylcholinesterase (BChE) degrades acetylcholine in addition to acetylcholinesterase (AChE) which is involved in embryonic development of limbs. Since BChE is expressed by osteoblast-like cells we asked whether it is functional in adult bone remodeling. We addressed this issue by analyzing BChE gene-deficient mice ...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2015.06.029

    authors： Haupt M,Kauschke V,Sender J,Kampschulte M,Kovtun A,Dürselen L,Heiss C,Lips KS

    更新日期：2015-11-01 00:00:00

  • Enhanced neutrophil autophagy and increased concentrations of IL-6, IL-8, IL-10 and MCP-1 in rheumatoid arthritis.

    abstract：:Rheumatoid arthritis (RA) is a high morbidity and disability disease with numerous inflammatory cells infiltrating in interstitial of articular cartilages and bones. As the most abundant inflammatory cells, neutrophil has been reported that their apoptosis changed gradually in the circumstance of RA. Apoptosis, one mo...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2018.09.011

    authors： An Q,Yan W,Zhao Y,Yu K

    更新日期：2018-12-01 00:00:00

  • Aspirin modified dendritic cells are potent inducers of allo-specific regulatory T-cells.

    abstract：:Salicylic acid (aspirin) is a widely used pharmacological agent with immunodmodulatory properties. Dendritic cells are key regulators of the immune response, and are capable of inducing hyporesponsiveness and regulatory activity in CD4+ T-cells. We have demonstrated that aspirin-treated dendritic cells are effective a...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：

    authors： Buckland M,Jago C,Fazekesova H,George A,Lechler R,Lombardi G

    更新日期：2006-12-20 00:00:00

  • Anti-inflammatory effects of 5-HT3 receptor antagonists in interleukin-1beta stimulated primary human chondrocytes.

    abstract：BACKGROUND:Chondrocytes are one of the main cell types involved in rheumatoid inflammation, releasing mediators which add to cartilage destruction, bone damage and consequently disability. Current evidence suggests that serotonin 5-HT(3) receptor antagonists (5-HT(3)RA) show anti-inflammatory and antioxidant properties...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2014.06.003

    authors： Stratz C,Anakwue J,Bhatia H,Pitz S,Fiebich BL

    更新日期：2014-09-01 00:00:00

  • MiR-let-7a regulates anti-citrullinated protein antibody-induced macrophage activation and correlates with the development of experimental rheumatoid arthritis.

    abstract：:Anti-citrullinated protein antibodies (ACPAs) are a key serological marker of rheumatoid arthritis (RA). To investigate whether miR-let7a downregulated ACPA-induced macrophage activation and arthritis, miR-let7a levels were assessed in the synovial fluid from patients with RA or osteoarthritis (OA). In addition, expre...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2017.08.001

    authors： Zhu W,Yu J,Qiu S,Liu H,Wang Y,Xu X,Shao L,Zhu L,Jiao Y,Liu F,Zhu X

    更新日期：2017-10-01 00:00:00

  • Paeoniflorin improves survival in LPS-challenged mice through the suppression of TNF-α and IL-1β release and augmentation of IL-10 production.

    abstract：:Lipopolysaccharide (LPS) plays an important role in Gram-negative bacteria-induced sepsis and multiple organ dysfunction syndrome, which are still the leading cause of high mortality in intensive care units. Although paeoniflorin (Pae) has reportedly exhibited anti-inflammatory effect and protection against immunologi...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2010.11.012

    authors： Cao W,Zhang W,Liu J,Wang Y,Peng X,Lu D,Qi R,Wang Y,Wang H

    更新日期：2011-02-01 00:00:00

  • Immunological evaluation of colonic delivered Hepatitis B surface antigen loaded TLR-4 agonist modified solid fat nanoparticles.

    abstract：:Hepatitis B is one of the leading liver diseases and remains a major global health problem. Currently available vaccines provide protection but often results in weaker/minimal mucosal immunity. Thus the present study is devoted to the development and in-vivo exploration of the colonically delivered biomimetic nanopart...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2016.08.007

    authors： Sahu KK,Pandey RS

    更新日期：2016-10-01 00:00:00

  • Neutrophil extracellular traps promote corneal neovascularization-induced by alkali burn.

    abstract：OBJECTIVES:To investigate the effects of neutrophil extracellular traps (NETs) on angiogenesis in vitro and in vivo and the regulatory role of mammalian target of rapamycin (mTOR) activity in it. METHODS:The regulatory role of mTOR in NETs formation was explored. In vitro, human neutrophils were pretreated with rapamy...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2020.106902

    authors： Yuan K,Zheng J,Huang X,Zhang Y,Han Y,Hu R,Jin X

    更新日期：2020-11-01 00:00:00

  • Keratinocyte nicotinic acetylcholine receptor activation modulates early TLR2-mediated wound healing responses.

    abstract：:The cholinergic anti-inflammatory pathway spans several macro- and micro-environments to control inflammation via α7 nicotinic acetylcholine receptors (nAChRs). Physiologic inflammation is necessary for normal wound repair and is triggered, in part, via Toll-like receptors (TLRs). Here, we demonstrate that keratinocyt...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2015.05.047

    authors： Kishibe M,Griffin TM,Radek KA

    更新日期：2015-11-01 00:00:00

  • Anti-arthritic effects of chlorogenic acid in interleukin-1β-induced rabbit chondrocytes and a rabbit osteoarthritis model.

    abstract：:Cartilage degradation is one of the pathological changes of osteoarthritis (OA), and accumulating evidence suggests an excess of matrix metalloproteinases (MMPs) plays a role in this cartilage breakdown. Here, we investigated the effects of chlorogenic acid (CGA) on the mRNA and protein expression of MMPs in interleuk...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2010.09.021

    authors： Chen WP,Tang JL,Bao JP,Hu PF,Shi ZL,Wu LD

    更新日期：2011-01-01 00:00:00

  • Interleukin-10 delivered by mesenchymal stem cells attenuates experimental autoimmune myocarditis.

    abstract：BACKGROUNDS:Autoimmune myocarditis is characterized by over-activated immune system attacking the cardiomyocytes, resulting in heart function decline. In the current study, we investigated the therapeutic advantages of delivering Interleukin-10 (IL-10) by mesenchymal stem cells (MSCs), both of which had immune suppress...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2020.106212

    authors： Shao M,Wang D,Zhou Y,Du K,Liu W

    更新日期：2020-04-01 00:00:00

  • Micheliolide alleviates hepatic steatosis in db/db mice by inhibiting inflammation and promoting autophagy via PPAR-γ-mediated NF-кB and AMPK/mTOR signaling.

    abstract：:The anti-inflammatory, immunomodulatory, and anticancer effects of micheliolide (MCL) isolated from Michelia champaca were previously reported, but its role and underlying mechanisms in relieving liver steatosis remain unclear. Herein, we investigated the effects of MCL on hepatic steatosis using a db/db mouse model a...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2018.03.036

    authors： Zhong J,Gong W,Chen J,Qing Y,Wu S,Li H,Huang C,Chen Y,Wang Y,Xu Z,Liu W,Li H,Long H

    更新日期：2018-06-01 00:00:00

  • A murine model of epicutaneous protein sensitization is useful to study efficacies of topical drugs in atopic dermatitis.

    abstract：:We studied the suitability of our murine model for the treatment trials of atopic dermatitis (AD). In this model topical application of ovalbumin (OVA) together with bacterial superantigen, staphylococcal enterotoxin B (SEB) induces a cutaneous disease resembling AD. Injured mouse skin was treated with three different...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2010.01.001

    authors： Lehto M,Savinko T,Wolff H,Kvist PH,Kemp K,Lauerma A,Alenius H

    更新日期：2010-04-01 00:00:00

  • Pure compound from Boswellia serrata extract exhibits anti-inflammatory property in human PBMCs and mouse macrophages through inhibition of TNFalpha, IL-1beta, NO and MAP kinases.

    abstract：:The aim of the present study is to probe the anti-inflammatory potential of the plant Boswellia serrata by studying the effect of the crude extract and the pure compound isolated from it on key inflammatory mediators like TNFalpha, IL-1beta, and NO thus enabling the understanding of the key signaling events involved. ...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2006.12.003

    authors： Gayathri B,Manjula N,Vinaykumar KS,Lakshmi BS,Balakrishnan A

    更新日期：2007-04-01 00:00:00

  • Inhibitory effects of eicosapentaenoic acid on lipopolysaccharide-induced activation in BV2 microglia.

    abstract：:Upon activation, microglia release proinflammatory mediators that play important roles in eliciting neuroinflammatory responses associated with neurodegenerative diseases. The anti-inflammatory properties of eicosapentaenoic acid (EPA) have been known, however, the effects responsible for lipopolysaccharide (LPS)-indu...

    journal_title：International immunopharmacology

    pub_type： 杂志文章

    doi：10.1016/j.intimp.2006.10.001

    authors： Moon DO,Kim KC,Jin CY,Han MH,Park C,Lee KJ,Park YM,Choi YH,Kim GY

    更新日期：2007-02-01 00:00:00