Abstract:
:Receptor tyrosine kinases (RTKs) are key regulators of normal cellular processes and have a critical role in the development and progression of many diseases. RTK ligand-induced stimulation leads to activation of the cytoplasmic kinase domain that controls the intracellular signalling. Although the kinase domain of RTKs has been extensively studied using X-ray analysis, the kinase insert domain (KID) and the C-terminal are partially or fully missing in all reported structures. We communicate the first structural model of the full-length RTK KIT cytoplasmic domain, a crucial target for cancer therapy. This model was achieved by integration of ab initio KID and C-terminal probe models into an X-ray structure, and by their further exploration through molecular dynamics (MD) simulation. An extended (2-µs) MD simulation of the proper model provided insight into the structure and conformational dynamics of the full-length cytoplasmic domain of KIT, which can be exploited in the description of the KIT transduction processes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Inizan F,Hanna M,Stolyarchuk M,Chauvot de Beauchêne I,Tchertanov Ldoi
10.1038/s41598-020-62460-7subject
Has Abstractpub_date
2020-03-25 00:00:00pages
5401issue
1issn
2045-2322pii
10.1038/s41598-020-62460-7journal_volume
10pub_type
杂志文章abstract::Plutella xylostella has become the major lepidopteran pest of Brassica owing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of entomopathogenic fungus, Metarhizium anisopliae, has broad-spectrum insecticidal effects. However, the interaction mechanism of destr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11298-7
更新日期:2017-09-08 00:00:00
abstract::Breast cancer (BC) is the leading cause of cancer death in women. Adipokines, and other inflammation molecules linked to adiposity, are suspected to be involved in breast carcinogenesis, however prospective findings are inconclusive. In a prospective nested case-control study within the EPIC-Varese cohort, we used con...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12703-x
更新日期:2017-10-05 00:00:00
abstract::Atmosphere-ocean coupling effect on the frequency distribution of tropical cyclones (TCs) and its future change is studied using an atmosphere and ocean coupled general circulation model (AOGCM). In the present climate simulation, the atmosphere-ocean coupling in the AOGCM improves biases in the AGCM such as the polew...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29800
更新日期:2016-07-15 00:00:00
abstract::TiNb6O17 and TiNb2O7 were synthesized using a solid-state method. The techniques were used to assess the electrochemical performance and lithium diffusion kinetics of TiNb6O17 related to the unit cell volume with TiNb2O7. The charge-discharge curves and cyclic voltammetry revealed TiNb6O17 to have a similar redox pote...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16711-9
更新日期:2017-11-30 00:00:00
abstract::Intergroup competition has an important impact on the survival and fitness of individuals in group-living species. However, factors influencing the probability of winning an encounter are not fully understood. We studied the influence of numerical advantage and location of the encounter on the chances of winning in ei...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28457
更新日期:2016-06-22 00:00:00
abstract::Alzheimer's disease (AD) is characterized by excessive production and deposition of amyloid-beta (Aβ) proteins as well as synapse dysfunction and loss. While soluble Aβ oligomers (AβOs) have deleterious effects on synapse function and reduce synapse number, the underlying molecular mechanisms are not well understood. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42548
更新日期:2017-02-13 00:00:00
abstract::Perceptual decisions are biased by recent perceptual history-a phenomenon termed 'serial dependence.' Here, we investigated what aspects of perceptual decisions lead to serial dependence, and disambiguated the influences of low-level sensory information, prior choices and motor actions. Participants discriminated whet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80128-0
更新日期:2021-01-12 00:00:00
abstract::Ancestral stress can program stress sensitivity and health trajectories across multiple generations. While ancestral stress is uncontrollable to the filial generations, it is critical to identify therapies that overcome transgenerational programming. Here we report that prenatal stress in rats generates a transgenerat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37814
更新日期:2016-11-24 00:00:00
abstract::Several microbes, including Staphylococcus epidermidis (S. epidermidis), a Gram-positive bacterium, live inside the human nasal cavity as commensals. The role of these nasal commensals in host innate immunity is largely unknown, although bacterial interference in the nasal microbiome may promote ecological competition...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27870
更新日期:2016-06-16 00:00:00
abstract::Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subject...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48364-1
更新日期:2019-08-14 00:00:00
abstract::Emerging concepts for on-chip biotechnologies aim to replace microfluidic flow by active, molecular-motor driven transport of cytoskeletal filaments, including applications in bio-simulation, biocomputation, diagnostics, and drug screening. Many of these applications require reliable detection, with minimal data acqui...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01092
更新日期:2013-01-01 00:00:00
abstract::Dust from grain and feed production may cause adverse health effects in exposed workers. In this study we explored circulating miRNAs as potential biomarkers of occupational grain dust exposure. Twenty-two serum miRNAs were analyzed in 44 grain dust exposed workers and 22 controls. Exposed workers had significantly up...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68296-5
更新日期:2020-07-09 00:00:00
abstract::Improving dewaterability of sludge is important for the disposal of sludge in wastewater treatment plants (WWTPs). This study, for the first time, investigated the Fe(II) activated oxidization processes in improving anaerobically digested sludge (ADS) dewaterability. The combination of Fe(II) (0-100 mg/g total solids ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24800
更新日期:2016-04-25 00:00:00
abstract::Materials and construction methods of nests vary between bird species and at present, very little is known about the relationships between architecture and function in these structures. This study combines computational and experimental techniques to study the structural biology of nests fabricated by the edible nest ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41245-7
更新日期:2019-03-18 00:00:00
abstract::Mechanochemical synthesis of Si/Cu3Si-based composite as negative electrode materials for lithium ion battery is investigated. Results indicate that CuO is decomposed and alloyed with Si forming amorphous Cu-Si solid solution due to high energy impacting during high energy mechanical milling (HEMM). Upon carbonization...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30703-3
更新日期:2018-08-23 00:00:00
abstract::Pancreatic cancer induces a substantial global burden. We examined its global incidence/mortality rates and their correlation with socioeconomic development (Human Development Index [HDI] and Gross Domestic Product [GDP] in 2000 as proxy measures). Data on age-standardized incidence/mortality rates in 2012 were retrie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02997-2
更新日期:2017-06-09 00:00:00
abstract::Although electroporation has been widely accepted as the main gene transfer tool, there is still considerable scope to improve the electroporation efficiency of exogenous DNAs into primary cells. Here, we developed a square-wave pulsing protocol using OptiMEM-GlutaMAX for highly efficient transfection of murine embryo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70258-w
更新日期:2020-08-07 00:00:00
abstract::Krüppel-like factor 6 (KLF6) is a transcription factor and tumor suppressor. We previously identified KLF6 as mediator of hepatocyte glucose and lipid homeostasis. The loss or reduction of KLF6 is linked to the progression of hepatocellular carcinoma, but its contribution to liver regeneration and repair in acute live...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08680-w
更新日期:2017-08-14 00:00:00
abstract::Acid-base homeostasis is critical for normal growth, development, and hearing function. The sodium-hydrogen exchanger 6 (NHE6), a protein mainly expressed in early and recycling endosomes, plays an important role in regulating organellar pH. Mutations in NHE6 cause complex, slowly progressive neurodegeneration. Little...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60262-5
更新日期:2020-02-27 00:00:00
abstract::Elastic waves are guided along finite structures such as cylinders, plates, or rods through reflection, refraction, and mode conversion at the interfaces. Such wave propagation is ubiquitous in the world around us, and studies of elastic waveguides first emerged in the later part of the 19(th) century. Early work on e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11112
更新日期:2015-06-08 00:00:00
abstract::For a high-performance cocatalyst-modified photocatalyst, an effective interfacial separation of photogenerated electron from its corresponding holes and its following reduction reaction at the active sites are highly required. However, it is difficult for a single-component cocatalyst to simultaneously realize the cr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13083
更新日期:2015-08-14 00:00:00
abstract::Drug-resistant tuberculosis (TB) is a serious global problem, and pathogen factors involved in the transmission of isoniazid (INH)-resistant TB have not been fully investigated. We performed whole genome sequencing of 332 clinical Mycobacterium tuberculosis (Mtb) isolates collected from patients newly diagnosed with s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51812-7
更新日期:2019-10-25 00:00:00
abstract::A biofilm has a unique structure composed of microorganisms, extracellular polymeric substances (EPSs), etc., and it is layered on a substrate in water. In material science, it is important to detect the biofilm formed on a surface to prevent biofouling. EPSs, the major component of the biofilm, mainly consist of poly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44167-6
更新日期:2019-05-30 00:00:00
abstract::The design of novel exciplex-forming co-host materials provides new opportunities to achieve high device performance of organic light emitting diodes (OLEDs), including high efficiency, low driving voltage and low efficiency roll-off. Here, we report a comprehensive study of exciplex-forming co-host system in OLEDs in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12059-2
更新日期:2017-09-20 00:00:00
abstract::ATP depletion and succinate accumulation during ischemia lead to oxidative damage to mammalian organs upon reperfusion. In contrast, freshwater turtles survive weeks of anoxia at low temperatures without suffering from oxidative damage upon reoxygenation, but the mechanisms are unclear. To determine how turtles surviv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39836-5
更新日期:2019-02-26 00:00:00
abstract::Recently, extracorporeal shock wave therapy (ESWT) has been shown to be a novel therapy for carpal tunnel syndrome (CTS). However, previous studies did not examine the diverse effects of different-session ESWT for different-grades CTS. Thus, we conducted a randomized, single-blind, placebo-controlled study. Sixty-nine...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/srep38344
更新日期:2016-12-02 00:00:00
abstract::Connectivity is fundamentally important for shaping the resilience of complex human and natural networks when systems are disturbed. Ecosystem resilience is, in part, shaped by the spatial arrangement of habitats, the permeability and fluxes between them, the stabilising functions performed by organisms, their dispers...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-80987-1
更新日期:2021-01-13 00:00:00
abstract::Interactions between diverse species that coexist in nature are of utmost interest in the field of ecology. Recent theoretical studies have shown that spatiality plays a key role in maintaining complex systems with multiple differing species. In these models, however, organisms move among habitats randomly, implying t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49143-8
更新日期:2019-09-02 00:00:00
abstract::In a haptic search task, one has to detect the presence of a target among distractors using the sense of touch. A salient target can be detected faster than a non-salient target. However, little is known about the exploration strategies that are used, especially in 3D search tasks where items are held in the hand. In ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06254
更新日期:2014-09-03 00:00:00
abstract::Land use change is one of the important aspects of the regional ecological restoration research. With remote sensing (RS) image in 2003, 2007 and 2012, using geographic information system (GIS) technologies, the land use pattern changes in Yimeng Mountain ecological restoration area in China and its driving force fact...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11038
更新日期:2015-06-05 00:00:00