Abstract：
:Several studies have suggested that there is a link between membrane attack complex (MAC) deposition in the retina and the progression of diabetic retinopathy (DR). Our recent investigation demonstrated that circulating IgG-laden extracellular vesicles contribute to an increase in retinal vascular permeability in DR through activation of the complement system. However, the mechanism through which extracellular vesicle-induced complement activation contributes to retinal vascular cytolytic damage in DR is not well understood. In this study, we demonstrate that IgG-laden extracellular vesicles in rat plasma activate the classical complement pathway, and in vitro Streptozotocin (STZ)-induced rat diabetic plasma results in MAC deposition and cytolytic damage in human retinal endothelial cells (HRECs). Moreover, removal of the plasma extracellular vesicles reduced the MAC deposition and abrogated cytolytic damage seen in HRECs. Together, the results of this study demonstrate that complement activation by IgG-laden extracellular vesicles in plasma could lead to MAC deposition and contribute to endothelium damage and progression of DR.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Huang C,Fisher KP,Hammer SS,Busik JVdoi
10.3390/ijms21051693subject
Has Abstractpub_date
2020-03-01 00:00:00issue
5issn
1422-0067pii
ijms21051693journal_volume
21pub_type
杂志文章abstract：:Docking-i.e., interaction of a small molecule (ligand) with a proteic structure (receptor)-represents the ground of drug action mechanism of the vast majority of bioactive chemicals. Ligand and receptor accommodate their geometry and energy, within this interaction, in the benefit of receptor-ligand complex. In an ind...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2017-06-27 00:00:00
abstract：:We evaluated the association between prostate cancer non-coding RNA 1 (PRNCR1) polymorphisms and the risk of developing gastric cancer (GC) and GC subgroups in Korea. A case-control study was conducted with 437 GC patients and 357 healthy controls using a TaqMan genotyping assay. A chi-squared test, binary logistic re...
journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2014-04-04 00:00:00
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journal_title：International journal of molecular sciences
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doi：10.3390/ijms12095577
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2017-12-01 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2018-04-29 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2021-01-06 00:00:00
abstract：:The kidney plays a primary role in maintaining homeostasis and detoxification of numerous hydrophilic xenobiotics as well as endogenous compounds. Because the kidney is exposed to a larger proportion and higher concentration of drugs and toxins than other organs through the secretion of ionic drugs by tubular organic ...
journal_title：International journal of molecular sciences
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更新日期：2016-11-01 00:00:00
abstract：:The activating leukocyte immunoglobulin-like receptors (LILRAs) play an important role in innate immunity. However, most of the LILRA members have not been characterized in avian species including chickens. The present study is the first attempt at cloning, structural analysis and functional characterization of two LI...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms19092710
更新日期：2018-09-11 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18061241
更新日期：2017-06-09 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197106
更新日期：2020-09-26 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020774
更新日期：2021-01-14 00:00:00
abstract：:Clinical conditions leading to chronic pain show important sex-related differences in the prevalence, severity, and degree of functional disability. Decades of epidemiological and clinical studies have demonstrated that women are more sensitive to pain than men. Arthritis, including rheumatoid arthritis (RA) and osteo...
journal_title：International journal of molecular sciences
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更新日期：2020-10-26 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2018-02-08 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2008-01-01 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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abstract：: Mono-saturated polyprenols (dolichols) have been found in almost all Eukaryotic cells, however, dolichols containing additional saturated bonds at the ω-end, have been identified in A.fumigatus and A.niger. Here we confirm using an LC-ESI-QTOF-MS analysis, that poly-saturated dolichols are abundant in other fila...
journal_title：International journal of molecular sciences
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更新日期：2019-06-21 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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abstract：:CDK5RAP3 was regarded as the most significant regulator of cellular responses against heat stress, which is associated with dysfunctions of the immune system and animal susceptibility to disease. Despite this, little known about how CDK5RAP3 regulates heat stress response. In this study, CDK5RAP3 conditional Knockout ...
journal_title：International journal of molecular sciences
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