Abstract:
: Mono-saturated polyprenols (dolichols) have been found in almost all Eukaryotic cells, however, dolichols containing additional saturated bonds at the ω-end, have been identified in A.fumigatus and A.niger. Here we confirm using an LC-ESI-QTOF-MS analysis, that poly-saturated dolichols are abundant in other filamentous fungi, Trichodermareesei, A.nidulans and Neurosporacrassa, while the yeast Saccharomyces cerevisiae only contains the typical mono-saturated dolichols. We also show, using differential scanning calorimetry (DSC) and fluorescence anisotropy of 1,6-diphenyl-l,3,5-hexatriene (DPH) that the structure of dolichols modulates the properties of membranes and affects the functioning of dolichyl diphosphate mannose synthase (DPMS). The activity of this enzyme from T.reesei and S. cerevisiae was strongly affected by the structure of dolichols. Additionally, the structure of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) model membranes was more strongly disturbed by the poly-saturated dolichols from Trichoderma than by the mono-saturated dolichols from yeast. By comparing the lipidome of filamentous fungi with that from S. cerevisiae, we revealed significant differences in the PC/PE ratio and fatty acids composition. Filamentous fungi differ from S. cerevisiae in the lipid composition of their membranes and the structure of dolichols. The structure of dolichols profoundly affects the functioning of dolichol-dependent enzyme, DPMS.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Gryz E,Perlińska-Lenart U,Gawarecka K,Jozwiak A,Piłsyk S,Lipko A,Jemiola-Rzeminska M,Bernat P,Muszewska A,Steczkiewicz K,Ginalski K,Długoński J,Strzalka K,Swiezewska E,Kruszewska JSdoi
10.3390/ijms20123043subject
Has Abstractpub_date
2019-06-21 00:00:00issue
12issn
1422-0067pii
ijms20123043journal_volume
20pub_type
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