Urinary NMR Profiling in Pediatric Acute Kidney Injury-A Pilot Study.


:Acute kidney injury (AKI) in critically ill children and adults is associated with significant short- and long-term morbidity and mortality. As serum creatinine- and urine output-based definitions of AKI have relevant limitations, there is a persistent need for better diagnostics of AKI. Nuclear magnetic resonance (NMR) spectroscopy allows for analysis of metabolic profiles without extensive sample manipulations. In the study reported here, we examined the diagnostic accuracy of NMR urine metabolite patterns for the diagnosis of neonatal and pediatric AKI according to the Kidney Disease: Improving Global Outcomes (KDIGO) definition. A cohort of 65 neonatal and pediatric patients (0-18 years) with established AKI of heterogeneous etiology was compared to both a group of apparently healthy children (n = 53) and a group of critically ill children without AKI (n = 31). Multivariate analysis identified a panel of four metabolites that allowed diagnosis of AKI with an area under the receiver operating characteristics curve (AUC-ROC) of 0.95 (95% confidence interval 0.86-1.00). Especially urinary citrate levels were significantly reduced whereas leucine and valine levels were elevated. Metabolomic differentiation of AKI causes appeared promising but these results need to be validated in larger studies. In conclusion, this study shows that NMR spectroscopy yields high diagnostic accuracy for AKI in pediatric patients.


Int J Mol Sci


Muhle-Goll C,Eisenmann P,Luy B,Kölker S,Tönshoff B,Fichtner A,Westhoff JH




Has Abstract


2020-02-11 00:00:00










  • Transcription Factor GarWRKY5 Is Involved in Salt Stress Response in Diploid Cotton Species (Gossypium aridum L.).

    abstract::Cotton is one of the most economically important crops in the world, and it is exposed to various abiotic stresses during its lifecycle, especially salt stress. However, the molecular mechanisms underlying cotton tolerance to salt stress are still not fully understood due to the complex nature of salt response. Theref...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Guo Q,Zhao L,Fan X,Xu P,Xu Z,Zhang X,Meng S,Shen X

    更新日期:2019-10-23 00:00:00

  • Molecular mechanisms of (R,R)ZX-5 on NO synthesis and its anti-angiogenic effect.

    abstract::(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western bl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Pan L,Hu JL,Wang WJ,Zhang XJ,Wei J,Liu ZD,Zhang YH,Xu HM

    更新日期:2012-01-01 00:00:00

  • Comprehensive analysis suggests overlapping expression of rice ONAC transcription factors in abiotic and biotic stress responses.

    abstract::NAC (NAM/ATAF/CUC) transcription factors comprise a large plant-specific gene family that contains more than 149 members in rice. Extensive studies have revealed that NAC transcription factors not only play important roles in plant growth and development, but also have functions in regulation of responses to biotic an...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sun L,Huang L,Hong Y,Zhang H,Song F,Li D

    更新日期:2015-02-17 00:00:00

  • Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood.

    abstract::Skeletal muscle has a remarkable plasticity, and its phenotype is strongly influenced by hormones, transcription factors, and physical activity. However, whether skeletal phenotype can be oriented or not during early embryonic stages has never been investigated. Here, we report that pyruvate as the only source of carb...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Bertrand-Gaday C,Letheule M,Blanchet E,Vernus B,Pessemesse L,Bonnet-Garnier A,Bonnieu A,Casas F

    更新日期:2020-09-29 00:00:00

  • Inherited Metabolic Disorders Presenting with Ataxia.

    abstract::Ataxia is a common clinical feature in inherited metabolic disorders. There are more than 150 inherited metabolic disorders in patients presenting with ataxia in addition to global developmental delay, encephalopathy episodes, a history of developmental regression, coarse facial features, seizures, and other types of ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Silver G,Mercimek-Andrews S

    更新日期:2020-08-01 00:00:00

  • Pygenic Acid A (PA) Sensitizes Metastatic Breast Cancer Cells to Anoikis and Inhibits Metastasis In Vivo.

    abstract::Metastasis is the main cause of cancer-related deaths. Anoikis is a type of apoptosis caused by cell detachment, and cancer cells become anoikis resistant such that they survive during circulation and can successfully metastasize. Therefore, sensitization of cancer cells to anoikis could prevent metastasis. Here, by s...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lim GE,Sung JY,Yu S,Kim Y,Shim J,Kim HJ,Cho ML,Lee JS,Kim YN

    更新日期:2020-11-10 00:00:00

  • High Expression of microRNA-143 is Associated with Favorable Tumor Immune Microenvironment and Better Survival in Estrogen Receptor Positive Breast Cancer.

    abstract::microRNA-143(miR-143) is a well-known tumor suppressive microRNA that exhibits anti-tumoral function by targeting KRAS signaling pathways in various malignancies. We hypothesized that miR-143 suppresses breast cancer progression by targeting KRAS and its effector molecules. We further hypothesized that high expression...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Tokumaru Y,Asaoka M,Oshi M,Katsuta E,Yan L,Narayanan S,Sugito N,Matsuhashi N,Futamura M,Akao Y,Yoshida K,Takabe K

    更新日期:2020-05-01 00:00:00

  • Levels of Interleukin-6 in Saliva, but Not Plasma, Correlate with Clinical Metrics in Huntington's Disease Patients and Healthy Control Subjects.

    abstract::Growing evidence suggests that inflammatory responses, in both the brain and peripheral tissues, contribute to disease pathology in Huntington's disease (HD), an inherited, progressive neurodegenerative disorder typically affecting adults in their 30-40 s. Hence, studies of inflammation-related markers in peripheral f...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Corey-Bloom J,Fischer RS,Kim A,Snell C,Parkin GM,Granger DA,Granger SW,Thomas EA

    更新日期:2020-09-02 00:00:00

  • Mitochondrial Quality Control in Age-Related Pulmonary Fibrosis.

    abstract::Idiopathic pulmonary fibrosis (IPF) is age-related interstitial lung disease of unknown etiology. About 100,000 people in the U.S have IPF, with a 3-year median life expectancy post-diagnosis. The development of an effective treatment for pulmonary fibrosis will require an improved understanding of its molecular patho...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Roque W,Cuevas-Mora K,Romero F

    更新日期:2020-01-18 00:00:00

  • SOD1 in Amyotrophic Lateral Sclerosis: "Ambivalent" Behavior Connected to the Disease.

    abstract::In 1993, Rosen and collaborators discovered that the gene encoding SOD1 has mutations in amyotrophic lateral sclerosis (ALS) patients; moreover, these mutations are found in the exon regions, suggesting that their toxic effects are the consequence of protein dysfunction with an increase of oxidative stress. While a cl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Pansarasa O,Bordoni M,Diamanti L,Sproviero D,Gagliardi S,Cereda C

    更新日期:2018-05-03 00:00:00

  • Studies to Prevent Degradation of Recombinant Fc-Fusion Protein Expressed in Mammalian Cell Line and Protein Characterization.

    abstract::Clipping of recombinant proteins is a major issue in animal cell cultures. A recombinant Fc-fusion protein, VEGFR1(D1-D3)-Fc expressed in CHOK1SV GS-KO cells was observed to be undergoing clippings in lab scale cultures. Partial cleaving of expressed protein initiated early on in cell culture and was observed to incre...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Chakrabarti S,Barrow CJ,Kanwar RK,Ramana V,Kanwar JR

    更新日期:2016-06-09 00:00:00

  • The Urinary Exosomal miRNA Expression Profile is Predictive of Clinical Response in Lupus Nephritis.

    abstract::Data on exosomal-derived urinary miRNAs have identified several miRNAs associated with disease activity and fibrosis formation, but studies on prognosis are lacking. We conducted a qPCR array screening on urinary exosomes from 14 patients with biopsy-proven proliferative lupus glomerulonephritis with a renal outcome o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Garcia-Vives E,Solé C,Moliné T,Vidal M,Agraz I,Ordi-Ros J,Cortés-Hernández J

    更新日期:2020-02-18 00:00:00

  • Regulation of Akt/FoxO3a/Skp2 Axis Is Critically Involved in Berberine-Induced Cell Cycle Arrest in Hepatocellular Carcinoma Cells.

    abstract::The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to induce cell cycle arrest...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li F,Dong X,Lin P,Jiang J

    更新日期:2018-01-23 00:00:00

  • Biohydrogen and Bioethanol Production from Biodiesel-Based Glycerol by Enterobacter aerogenes in a Continuous Stir Tank Reactor.

    abstract::Crude glycerol from the biodiesel manufacturing process is being produced in increasing quantities due to the expanding number of biodiesel plants. It has been previously shown that, in batch mode, semi-anaerobic fermentation of crude glycerol by Enterobacter aerogenes can produce biohydrogen and bioethanol simultaneo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Jitrwung R,Yargeau V

    更新日期:2015-05-11 00:00:00

  • Evaluation of Magnetic Nanoparticle-Labeled Chondrocytes Cultivated on a Type II Collagen-Chitosan/Poly(Lactic-co-Glycolic) Acid Biphasic Scaffold.

    abstract::Chondral or osteochondral defects are still controversial problems in orthopedics. Here, chondrocytes labeled with magnetic nanoparticles were cultivated on a biphasic, type II collagen-chitosan/poly(lactic-co-glycolic acid) scaffold in an attempt to develop cultures with trackable cells exhibiting growth, differentia...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Su JY,Chen SH,Chen YP,Chen WC

    更新日期:2017-01-04 00:00:00

  • Multiple Links between HD-Zip Proteins and Hormone Networks.

    abstract::HD-Zip proteins are unique to plants, and contain a homeodomain closely linked to a leucine zipper motif, which are involved in dimerization and DNA binding. Based on homology in the HD-Zip domain, gene structure and the presence of additional motifs, HD-Zips are divided into four families, HD-Zip I⁻IV. Phylogenetic a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Sessa G,Carabelli M,Possenti M,Morelli G,Ruberti I

    更新日期:2018-12-14 00:00:00

  • AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis.

    abstract::Although most osmotic stresses are harmful to plant growth and development, certain drought- or polyethylene glycol (PEG)-induced mild osmotic stresses promote plant root growth. The underlying regulatory mechanisms of this response remain elusive. Here, we report that the GLYCOGEN SYNTHASE KINASE 3 (GSK3) genes ARABI...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dong L,Wang Z,Liu J,Wang X

    更新日期:2020-06-02 00:00:00

  • Inflammatory Cutaneous Diseases in Renal Transplant Recipients.

    abstract::Kidney transplant recipients frequently suffer from skin infections and malignancies, possibly due to the effects of long-term immunosuppressive therapy. While the relationships between immunosuppression and these pathological conditions have been widely investigated, little is known about the relative incidence and c...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Savoia P,Cavaliere G,Zavattaro E,Veronese F,Fava P

    更新日期:2016-08-19 00:00:00

  • Injectable Gel Form of a Decellularized Bladder Induces Adipose-Derived Stem Cell Differentiation into Smooth Muscle Cells In Vitro.

    abstract::Biologic scaffolds composed of extracellular matrix components have been proposed to repair and reconstruct a variety of tissues in clinical and pre-clinical studies. Injectable gels can fill and conform any three-dimensional shape and can be delivered to sites of interest by minimally invasive techniques. In this stu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Moreno-Manzano V,Zaytseva-Zotova D,López-Mocholí E,Briz-Redón Á,Løkensgard Strand B,Serrano-Aroca Á

    更新日期:2020-11-15 00:00:00

  • A Current Overview of the Biological and Cellular Effects of Nanosilver.

    abstract::Nanosilver plays an important role in nanoscience and nanotechnology, and is becoming increasingly used for applications in nanomedicine. Nanosilver ranges from 1 to 100 nanometers in diameter. Smaller particles more readily enter cells and interact with the cellular components. The exposure dose, particle size, coati...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Cameron SJ,Hosseinian F,Willmore WG

    更新日期:2018-07-12 00:00:00

  • The Biocontrol Root-Oomycete, Pythium Oligandrum, Triggers Grapevine Resistance and Shifts in the Transcriptome of the Trunk Pathogenic Fungus, Phaeomoniella Chlamydospora.

    abstract::The worldwide increase in grapevine trunk diseases, mainly esca, represents a major threat for vineyard sustainability. Biocontrol of a pioneer fungus of esca, Phaeomoniella chlamydospora, was investigated here by deciphering the tripartite interaction between this trunk-esca pathogen, grapevine and the biocontrol-oom...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Yacoub A,Magnin N,Gerbore J,Haidar R,Bruez E,Compant S,Guyoneaud R,Rey P

    更新日期:2020-09-19 00:00:00

  • miR-7 Knockdown by Peptide Nucleic Acids in the Ascidian Ciona intestinalis.

    abstract::Peptide Nucleic Acids (PNAs) are synthetic mimics of natural oligonucleotides, which bind complementary DNA/RNA strands with high sequence specificity. They display numerous advantages, but in vivo applications are still rare. One of the main drawbacks of PNAs application is the poor cellular uptake that could be over...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mercurio S,Cauteruccio S,Manenti R,Candiani S,Scarì G,Licandro E,Pennati R

    更新日期:2019-10-16 00:00:00

  • Thyroid Disrupting Chemicals.

    abstract::Endocrine disruptor compounds are exogenous agents able to interfere with a gland function, exerting their action across different functional passages, from the synthesis to the metabolism and binding to receptors of the hormone produced. Several issues, such as different levels and time of exposure and different acti...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Calsolaro V,Pasqualetti G,Niccolai F,Caraccio N,Monzani F

    更新日期:2017-12-01 00:00:00

  • The between Now and Then of Lung Cancer Chemotherapy and Immunotherapy.

    abstract::Lung cancer is the most common cancer worldwide. Disappointingly, despite great effort in encouraging screening or, at least, a close surveillance of high-risk individuals, most of lung cancers are diagnosed when already surgically unresectable because of local advancement or metastasis. In these cases, the treatment ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Visconti R,Morra F,Guggino G,Celetti A

    更新日期:2017-06-27 00:00:00

  • Ceramide Metabolism Balance, a Multifaceted Factor in Critical Steps of Breast Cancer Development.

    abstract::Ceramides are key lipids in energetic-metabolic pathways and signaling cascades, modulating critical physiological functions in cells. While synthesis of ceramides is performed in endoplasmic reticulum (ER), which is altered under overnutrition conditions, proteins associated with ceramide metabolism are located on me...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: García-González V,Díaz-Villanueva JF,Galindo-Hernández O,Martínez-Navarro I,Hurtado-Ureta G,Pérez-Arias AA

    更新日期:2018-08-26 00:00:00

  • Efficient and Stable Delivery of Multiple Genes to Fish Cells by a Modified Recombinant Baculovirus System.

    abstract::The recombinant baculovirus has been widely used as an efficient tool to mediate gene delivery into mammalian cells but has barely been used in fish cells. In the present study, we constructed a recombinant baculovirus containing the dual-promoter cytomegalovirus (CMV) and white spot syndrome virus (WSSV) immediate-ea...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang Q,Fang J,Pan Q,Wang Y,Xue T,Li L,Chen T

    更新日期:2018-11-27 00:00:00

  • Evolution of Disease Defense Genes and Their Regulators in Plants.

    abstract::Biotic stresses do damage to the growth and development of plants, and yield losses for some crops. Confronted with microbial infections, plants have evolved multiple defense mechanisms, which play important roles in the never-ending molecular arms race of plant⁻pathogen interactions. The complicated defense systems i...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zhang R,Zheng F,Wei S,Zhang S,Li G,Cao P,Zhao S

    更新日期:2019-01-15 00:00:00

  • Aminolevulinic Acid-Based Tumor Detection and Therapy: Molecular Mechanisms and Strategies for Enhancement.

    abstract::Aminolevulinic acid (ALA) is the first metabolite in the heme biosynthesis pathway in humans. In addition to the end product heme, this pathway also produces other porphyrin metabolites. Protoporphyrin (PpIX) is one heme precursor porphyrin with good fluorescence and photosensitizing activity. Because tumors and other...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Yang X,Palasuberniam P,Kraus D,Chen B

    更新日期:2015-10-28 00:00:00

  • Characterization and comparison of Fumonisin B(1)-protein conjugates by six methods.

    abstract::In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (Fumonisin B(1), FB(1)) was coupled to ovalbumin (OVA) and bovine serum albumin (BSA), respectively by a short cross-linker reagent (glutaraldehyde, GA). T...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang Y,He CH,Zheng H,Zhang HB

    更新日期:2012-01-01 00:00:00

  • Small Extracellular Vesicles Isolated from Serum May Serve as Signal-Enhancers for the Monitoring of CNS Tumors.

    abstract::Liquid biopsy-based methods to test biomarkers (e.g., serum proteins and extracellular vesicles) may help to monitor brain tumors. In this proteomics-based study, we aimed to identify a characteristic protein fingerprint associated with central nervous system (CNS) tumors. Overall, 96 human serum samples were obtained...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dobra G,Bukva M,Szabo Z,Bruszel B,Harmati M,Gyukity-Sebestyen E,Jenei A,Szucs M,Horvath P,Biro T,Klekner A,Buzas K

    更新日期:2020-07-28 00:00:00