Abstract：
:Somatic cell nuclear transfer (SCNT) has potential applications in agriculture and biomedicine, but the efficiency of cloning is still low. In this study, the transcriptional profiles in cloned and fertilized embryos were measured and compared by RNA sequencing. The 2-cell embryos were detected to identify the earliest transcriptional differences between embryos derived through IVF and SCNT. As a result, 364 genes showed decreased expression in cloned 2-cell embryos and were enriched in "intracellular protein transport" and "ubiquitin mediated proteolysis". In blastocysts, 593 genes showed decreased expression in cloned blastocysts and were enriched in "RNA binding", "nucleotide binding", "embryo development", and "adherens junction". We identified 14 development related genes that were not activated in the cloned embryos. Then, 68 and 245 long non-coding RNAs were recognized abnormally expressed in cloned 2-cell embryos and cloned blastocysts, respectively. Furthermore, we found that incomplete RNA-editing occurred in cloned embryos and might be caused by decreased ADAR expression. In conclusion, our study revealed the abnormal transcripts and deficient RNA-editing sites in cloned embryos and provided new data for further mechanistic studies of somatic nuclear reprogramming.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhang L,Yu M,Xu H,Wei X,Liu Y,Huang C,Chen H,Guo Zdoi
10.1016/j.ijbiomac.2020.02.026subject
Has Abstractpub_date
2020-05-01 00:00:00pages
492-500eissn
0141-8130issn
1879-0003pii
S0141-8130(19)35970-7journal_volume
150pub_type
杂志文章abstract：:Phauda flammans Walker belongs to Phaudidae (Lepidoptera), which is a holometabolous and leaf-eating pest that harms trees. So far, there is no mitochondrial (mt) genome reported of Phaudidae. Herein, we sequenced and annotated the complete mt genome of P. flammans representing the first mt genome of Phaudidae and pre...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.01.307
更新日期：2020-04-15 00:00:00
abstract：:In this study, highly porous N/S doped magnetic carbon aerogel (N/S-MCA) was successfully fabricated using sugarcane bagasse based cellulose and used for the removal of bisphenol‑A (BPA) from aqueous solution. The fabricated N/S-MCA nanocomposite derived from cellulose was characterized by various analytical technique...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.004
更新日期：2019-07-01 00:00:00
abstract：:alpha-helices are the most common secondary structures in observed proteins. However, they are not always found in ideal helical conformation and they often exhibit structural distortions. Quantification of these irregularities become essential in understanding the packing of helices and therefore, their role in the f...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(96)01106-3
更新日期：1996-08-01 00:00:00
abstract：:Fibrinolytic enzymes are proteases responsible for cleavage of fibrin mesh in thrombus clots, which are the primary causative agents in cardiovascular diseases. Developing safe, effective and cheap thrombolytic agents are important for prevention and cure of thrombosis. Although a wide variety of sources have been dis...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.07.303
更新日期：2020-11-15 00:00:00
abstract：:The effect of gamma irradiation, with doses from 10(2) to 10(3) kGy, on the dielectric relaxation time of solid-state collagen was studied. Temperature measurements of the relaxation time were made over a range of frequency of the electric field from 10(1) to 10(5) Hz and at temperatures from 298 to 480 K. The samples...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(95)93510-5
更新日期：1995-02-01 00:00:00
abstract：:Conjugation of DNA with testosterone and it aliphatic dimer (alip) and aromatic dimer (arom) was investigated in aqueous solution at pH 7.4. Multiple spectroscopic methods, transmission electron microscopy (TEM) and molecular modeling were used to characterize steroid-DNA binding and DNA morphology. Spectroscopic anal...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.09.090
更新日期：2017-02-01 00:00:00
abstract：:Ubb+1, a ubiquitin (Ub) mutant protein originating from misreading of the Ub B gene, is found accumulated in brain tissues of Alzheimer's disease patients. The mutant attracts strong interest due to its possible participation in the molecular events leading to neurodegeneration. Ubb+1 is composed of the globular domai...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.11.121
更新日期：2018-03-01 00:00:00
abstract：:A response surface methodology was used to optimize the parameters for extracting the polysaccharides from tobacco waste (TWPs) using hot water. The extraction process, carried out under the following optimized parameters: an extraction temperature of 90°C, a ratio of water to raw material of 54, and an extraction tim...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.05.069
更新日期：2016-10-01 00:00:00
abstract：:There have been few reports of complete mitochondrial genomes (mitogenomes) of scale insects, and it has been indicated that complex and novel structures in their mitogenomes may lead to difficulties in sequencing, assembly and annotation. Transfer RNAs (tRNAs) usually possess typical cloverleaf secondary structures, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.257
更新日期：2020-05-07 00:00:00
abstract：:Cellulose-based hydrogels have wide applications in tissue engineering and controlled delivery systems. In this study, chloramphenicol (CAP) loaded 2,3 dialdehyde cellulose (DABC) hydrogel membranes were prepared, characterized and their antibacterial efficacy was evaluated. Bacterial cellulose (BC) secreted by Acetob...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.03.003
更新日期：2014-06-01 00:00:00
abstract：:Three 4-(1H-imidazo[4,5-f]-1,10-phenanthrolin-2-yl)phenol derivatives-based platinum (II) complexes have been synthesized, and their G-quadruplex DNAs-binding interactions, telomerase inhibition, antiproliferative activity, and cell cycle arrest were studied. Three complexes show the preference for stabilizing h-telo,...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.01.004
更新日期：2013-04-01 00:00:00
abstract：:Morinda officinalis is an important traditional tonic herbal medicine. In the present study, we found that crude polysaccharides extracted from M. officinalis, named MO90, could significantly increase the bone mineral density (BMD) of the whole femur, distal femur, and proximal femur in ovariectomized (OVX) rats. In a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.084
更新日期：2019-07-15 00:00:00
abstract：:The effects of different pretreatments on yield and composition of extraction, physicochemical, and rheological properties of duck feet gelatin (DFG) were investigated. Gelatins were extracted from the whole feet of Pekin duck with an average yield of 4.09%, 3.65%, and 5.75% for acidic (Ac-DFG), alkaline (Al-DFG), and...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.139
更新日期：2017-05-01 00:00:00
abstract：:The unperturbed dimensions of poly-alpha-D-galacturonic acid (PGA) as a function of the degree of polymerization (n) and degree of ionization (%GalA-) have been determined by molecular mechanics and Monte Carlo methods. Chain extensions appear to depend substantially on contributions arising from local redistributions...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(95)92688-m
更新日期：1995-06-01 00:00:00
abstract：:In this study, the heterologous expression and biochemical characterization of a thermostable α-amylase from Geobacillus sp. GS33 was investigated. The recombinant α-amylase was overexpressed in Escherichia coli BL21 (λDE) and purified via anion exchange and size-exclusion chromatography. The purified α-amylase had a ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.171
更新日期：2020-12-01 00:00:00
abstract：:The structure of a ubiquitin-like protein, small ubiquitin-related modifier-1 (SUMO-1), was earlier determined using homonuclear nuclear magnetic resonance (NMR) spectroscopy, since the spectral quality of the protein was not suitable for heteronuclear NMR data collection. In this study, a slightly different construct...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(00)00169-0
更新日期：2001-03-14 00:00:00
abstract：:Effective hemorrhage control is pivotal for decreasing the trauma death both in civilian and military but has proven to be dauntingly challenging, especially for solid viscera and artery trauma. Here we report the fabrication of a novel starch-based hemostat, sodium trimethaphosphate (STMP)-crosslinked starch/hyaluron...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.018
更新日期：2020-05-06 00:00:00
abstract：:For saccharifying starch in one step, a chimeric biocatalyst (Amy-Glu) was generated from engineered α-amylase (Ba-Gt-amy) of Bacillus acidicola and glucoamylase (Glu) gene of Aspergillus niger. In order to join two enzymes, a linker peptide of 25 amino acids was used. Chimeric Amy-Glu was expressed in E. coli. Glu is...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.02.083
更新日期：2017-06-01 00:00:00
abstract：:An efficient method was developed to prepare oxidized potato starch under the pasting point by Electro-Fenton system, in which a complex of sodium citrate and Fe2+ (SC-Fe2+) was used as catalyst, and H2O2 was used as oxidant. In the preparation process the oxidation degree of starch was controlled by the molar ratios ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.012
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:Long non-coding RNAs are emerging as key molecules in cancer progression. LncRNA-CCAL has shown to be highly expressed and important in regulating CRC and osteosarcoma development. Nevertheless, the expression and mechanism of CCAL in HCC is still not well understood. METHODS:qRT-PCR and ISH were used to eva...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.110
更新日期：2018-04-01 00:00:00
abstract：:This study aimed to evaluate the in vitro protective effect of topical treatment with a native sulfated polysaccharide of G. caudata (SP-Gc), hydrolyzed (H-SP-Gc), or desulfated (D-SP-Gc) polysaccharide of Gracilaria caudata in esophageal biopsies obtained from GERD patients. Biopsies were obtained from nonerosive ref...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.074
更新日期：2020-05-01 00:00:00
abstract：:Clinical application of curcumin for Alzheimer's disease treatment is severely limited with regard to its poor bioavailability, high rate of metabolism, and instability under neutral condition. In the current study, we designed three compounds in which the diketone moiety of curcumin was replaced by cyclohexanone. In ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.038
更新日期：2015-01-01 00:00:00
abstract：:The present study describes the physical, antioxidant, and antimicrobial properties of biodegradable films prepared by incorporating different concentrations (1, 2, and 3% v/v) of Zataria multiflora Boiss (avishan-e shirazi) essential oil (ZEO) into carboxymethyl cellulose (CMC) film. The films' tensile strength, elon...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.09.006
更新日期：2015-01-01 00:00:00
abstract：:Two laboratory-made cationic starch-based flocculants (St-CTA and St-AD) with different chain architectures were used to simultaneously remove phosphorus and turbidity from two simulated wastewaters and one actual wastewater with laboratory and pilot scales, respectively, in conjunction with FeCl3. A commercial polyac...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.176
更新日期：2021-01-15 00:00:00
abstract：:Survival of the Acinetobacter baumannii inside host requires different micronutrients such as iron, but their bioavailability is limited because of nutritional immunity created by host. A. baumannii has to develop mechanisms to acquire nutrient iron during infection. The present study is an attempt to identify membran...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.173
更新日期：2019-03-15 00:00:00
abstract：:This study investigated the effect of Lactobacillus fermentum fermentation treatment on the gastrointestinal digestion and fermentation in vitro of polysaccharides from longan pulp. Polysaccharide isolated from unfermented and fermented longan pulp named LP and LP-F, respectively. The molecular weight of LP-F declined...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.01.059
更新日期：2020-03-15 00:00:00
abstract：:While the cashew culture is focused on processing and commercialization of cashew nuts, the pseudofruit (cashew apples) - highly perishable and of limited acceptance - are mostly wasted. The cashew tree pruning fiber (CTPF) is another interesting cashew byproduct. In this study, films have been made from bacterial cel...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.269
更新日期：2020-10-15 00:00:00
abstract：:Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at -20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtaine...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.141
更新日期：2020-08-01 00:00:00
abstract：:Small Hsps represent a variation on the theme of protection of proteins from irreversible aggregation by reversible interaction with chaperone proteins. While different sHsps are highly heterogeneous in sequence and size, the common trait is the presence of a conserved alpha-crystallin domain. In addition sHsps assemb...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.01.013
更新日期：2006-03-30 00:00:00
abstract：:Fluorescence quenching data on interaction of a fungicide methyl thiophanate (MT) with human serum albumin (HSA) elucidated a primary binding site at sub-domain IIA. Stern-Volmer algorithm and double log plot revealed the binding affinity (K(a)) and capacity (n) of HSA as 1.65 x 10(4)M(-1) and 1.0 (r(2)=0.99), respect...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.03.020
更新日期：2010-07-01 00:00:00
