RNA sequencing revealed the abnormal transcriptional profile in cloned bovine embryos.

Abstract:

:Somatic cell nuclear transfer (SCNT) has potential applications in agriculture and biomedicine, but the efficiency of cloning is still low. In this study, the transcriptional profiles in cloned and fertilized embryos were measured and compared by RNA sequencing. The 2-cell embryos were detected to identify the earliest transcriptional differences between embryos derived through IVF and SCNT. As a result, 364 genes showed decreased expression in cloned 2-cell embryos and were enriched in "intracellular protein transport" and "ubiquitin mediated proteolysis". In blastocysts, 593 genes showed decreased expression in cloned blastocysts and were enriched in "RNA binding", "nucleotide binding", "embryo development", and "adherens junction". We identified 14 development related genes that were not activated in the cloned embryos. Then, 68 and 245 long non-coding RNAs were recognized abnormally expressed in cloned 2-cell embryos and cloned blastocysts, respectively. Furthermore, we found that incomplete RNA-editing occurred in cloned embryos and might be caused by decreased ADAR expression. In conclusion, our study revealed the abnormal transcripts and deficient RNA-editing sites in cloned embryos and provided new data for further mechanistic studies of somatic nuclear reprogramming.

journal_name

Int J Biol Macromol

authors

Zhang L,Yu M,Xu H,Wei X,Liu Y,Huang C,Chen H,Guo Z

doi

10.1016/j.ijbiomac.2020.02.026

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

492-500

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)35970-7

journal_volume

150

pub_type

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