RNA sequencing revealed the abnormal transcriptional profile in cloned bovine embryos.


:Somatic cell nuclear transfer (SCNT) has potential applications in agriculture and biomedicine, but the efficiency of cloning is still low. In this study, the transcriptional profiles in cloned and fertilized embryos were measured and compared by RNA sequencing. The 2-cell embryos were detected to identify the earliest transcriptional differences between embryos derived through IVF and SCNT. As a result, 364 genes showed decreased expression in cloned 2-cell embryos and were enriched in "intracellular protein transport" and "ubiquitin mediated proteolysis". In blastocysts, 593 genes showed decreased expression in cloned blastocysts and were enriched in "RNA binding", "nucleotide binding", "embryo development", and "adherens junction". We identified 14 development related genes that were not activated in the cloned embryos. Then, 68 and 245 long non-coding RNAs were recognized abnormally expressed in cloned 2-cell embryos and cloned blastocysts, respectively. Furthermore, we found that incomplete RNA-editing occurred in cloned embryos and might be caused by decreased ADAR expression. In conclusion, our study revealed the abnormal transcripts and deficient RNA-editing sites in cloned embryos and provided new data for further mechanistic studies of somatic nuclear reprogramming.


Int J Biol Macromol


Zhang L,Yu M,Xu H,Wei X,Liu Y,Huang C,Chen H,Guo Z




Has Abstract


2020-05-01 00:00:00












  • Cu(II) ion loading in silk fibroin scaffolds with silk I structure.

    abstract::Metal ions play important roles in the diverse biochemical reactions associated with many cell signalling pathways. The modification of biomaterials with metal ions may offer a promising approach to stimulate cellular activity for improving tissue regeneration. Here, copper ion loading as a potential therapeutic agent...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hua J,You H,Li X,You R,Ma L

    更新日期:2020-05-05 00:00:00

  • Heteronuclear nuclear magnetic resonance assignments, structure and dynamics of SUMO-1, a human ubiquitin-like protein.

    abstract::The structure of a ubiquitin-like protein, small ubiquitin-related modifier-1 (SUMO-1), was earlier determined using homonuclear nuclear magnetic resonance (NMR) spectroscopy, since the spectral quality of the protein was not suitable for heteronuclear NMR data collection. In this study, a slightly different construct...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Jin C,Shiyanova T,Shen Z,Liao X

    更新日期:2001-03-14 00:00:00

  • Low molecular weight chitosan nanoparticles for CpG oligodeoxynucleotides delivery: Impact of molecular weight, degree of deacetylation, and mannosylation on intracellular uptake and cytokine induction.

    abstract::Although synthetic CpG oligodeoxynucleotides (ODNs) have shown substantial potential as immunotherapeutic agents, their effective intracellular delivery remains challenging. In this work, nanoparticles prepared from low-molecular weight (LMW) chitosans were investigated as CpG ODN delivery systems. Chitosan samples wi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Babii O,Wang Z,Liu G,Martinez EC,van Drunen Littel-van den Hurk S,Chen L

    更新日期:2020-09-15 00:00:00

  • Chemical and biological characterization of polysaccharides isolated from Ilex paraguariensis A. St.-Hil.

    abstract::The potential gastroprotection of polysaccharides (SP) isolated from maté (Ilex paraguariensis) leaves of different growth stages, under different sunlight conditions and of processing methods were evaluated. The SP consist of type I arabinogalactan (AG1) containing a (1→4)-linked β-Galp chain, with substituents of ar...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Maria-Ferreira D,Dartora N,da Silva LM,Pereira IT,de Souza LM,Ritter DS,Iacomini M,Werner MF,Sassaki GL,Baggio CH

    更新日期:2013-08-01 00:00:00

  • Comparison of laccase-catalyzed cross-linking of organosolv lignin and lignosulfonates.

    abstract::Lignin, an underutilized by-product from chemical pulping of wood, can be modified enzymatically through oxidation by laccase. However, little is known about the molecular details surrounding the cross-linking which is a result of the oxidation. To reduce this lack of knowledge, we used oxygen consumption rate data, p...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Gillgren T,Hedenström M,Jönsson LJ

    更新日期:2017-12-01 00:00:00

  • Biologically active polysaccharide from edible mushrooms: A review.

    abstract::Mushrooms are renewable natural gift for humankind, furnished with unique taste, flavor and medicinal properties. For the last few decades study of mushroom polysaccharides has become a matter of great interest to the researchers for their immunomodulating, antimicrobial, antioxidant, anticancer, and antitumor propert...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Maity P,Sen IK,Chakraborty I,Mondal S,Bar H,Bhanja SK,Mandal S,Maity GN

    更新日期:2021-01-16 00:00:00

  • Thermal stress resistance and aging effects of Panax notoginseng polysaccharides on Caenorhabditis elegans.

    abstract::Panax notoginseng attract public attention due to their potential biomedical properties and corresponding health benefits. The present study investigated the anti-aging and thermal stress resistance effects of polysaccharides from P. notoginseng on Caenorhabditis elegans. Results showed polysaccharides had little scav...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Feng S,Cheng H,Xu Z,Shen S,Yuan M,Liu J,Ding C

    更新日期:2015-11-01 00:00:00

  • One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.

    abstract::Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability. To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chrom...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Gopalakannan V,Viswanathan N

    更新日期:2016-02-01 00:00:00

  • Preparation and characterization of poly(ε-caprolactone) scaffolds modified with cell-loaded fibrin gel.

    abstract::Poly(ε-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and m...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Malikmammadov E,Tanir TE,Kiziltay A,Hasirci N

    更新日期:2019-03-15 00:00:00

  • Study the effect of trypsin enzyme activity on the screening of applying frontal affinity chromatography.

    abstract::Frontal affinity chromatography (FAC) combined with enzyme has been widely used for drug screening. In this paper, the effect of target enzyme activity on screening of bioactive compounds was studied through applying FAC. Trypsin with different degree of inactivation were prepared as target enzyme by thermal denaturat...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Qian J,Zhao C,Tong J,Jiang S,Zhang Z,Lu S,Guo H

    更新日期:2019-10-15 00:00:00

  • Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.

    abstract::The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications. Arabinoxylan extracted from psyllium was carboxymethylated by reacting with monochloroacetic acid under alkaline co...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Bhatia M,Ahuja M

    更新日期:2015-01-01 00:00:00

  • Evaluation of the fluid uptake kinetics and drug release from gellan gum tablets containing metronidazole.

    abstract::In this study, the fluid uptake (swelling) kinetics and disintegrant properties of gellan gum in metronidazole tablets were evaluated in both simulated gastric and intestinal fluids (SGF and SIF respectively) without enzymes. The mechanical properties as well as the disintegration and dissolution profile of the tablet...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Emeje MO,Franklin-Ude PI,Ofoefule SI

    更新日期:2010-08-01 00:00:00

  • Effects of trehalose and sorbitol on the activity and structure of Pseudomonas cepacia lipase: spectroscopic insight.

    abstract::The stability of enzymes with no reduction in their catalytic activity still remains a critical issue in industrial applications. Naturally occurring osmolytes are commonly used as protein stabilizers. In this study we have investigated the effects of sorbitol and trehalose on the structural stability and activity of ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Azizi A,Ranjbar B,Khajeh K,Ghodselahi T,Hoornam S,Mobasheri H,Ganjalikhany MR

    更新日期:2011-11-01 00:00:00

  • Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii.

    abstract::A water-soluble polysaccharide (EPA-1) from Pleurotus eryngii was obtained using DEAE-52 and Sephadex G-50 columns. The properties, structure and immunomodulatory activity of EPA-1 were studied. The results demonstrated that EPA-1 was a homogeneous polysaccharide with the molecular weight of 9.97×104Da. EPA-1 consiste...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Xu D,Wang H,Zheng W,Gao Y,Wang M,Zhang Y,Gao Q

    更新日期:2016-11-01 00:00:00

  • The development of advanced cellulosic fibres.

    abstract::For the majority of the last century, commercial routes to regenerated cellulose fibres have coped with the difficulties of making a good cellulose solution by using an easy to dissolve derivative (e.g. xanthate in the case of viscose rayon) or complex (e.g. cuprammonium rayon). For the purposes of this paper, advance...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Woodings CR

    更新日期:1995-12-01 00:00:00

  • Radiation-mediated molecular weight reduction and structural modification in carrageenan potentiates improved photosynthesis and secondary metabolism in peppermint (Mentha piperita L.).

    abstract::In an attempt to gain insights into the possible relationship between the irradiation-mediated molecular weight reduction and structural modification and the growth-promotion activity, characterization of the polysaccharide before and after irradiation was carried out through Fourier Transform Infrared (FT-IR), Ultrav...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ahmad B,Jahan A,Sadiq Y,Shabbir A,Jaleel H,Khan MMA

    更新日期:2019-03-01 00:00:00

  • Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 β-N-acetylhexosaminidase.

    abstract::β-N-Acetylhexosaminidases (CAZy GH20, EC are exo-glycosidases specific for cleaving N-acetylglucosamine and N-acetylgalactosamine moieties of various substrates. The β-N-acetylhexosaminidase from the filamentous fungus Talaromyces flavus (TfHex), a model enzyme in this study, has a broad substrate flexibilit...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kapešová J,Petrásková L,Kulik N,Straková Z,Bojarová P,Markošová K,Rebroš M,Křen V,Slámová K

    更新日期:2020-10-15 00:00:00

  • Study on the derivation of cassava residue and its application in surface sizing.

    abstract::In order to maximize value and minimize waste, carboxymethyl cellulose (CMC) and carboxymethyl starch (CMS) was synthesized via solvent method using cassava residue as raw material. The alkalization time, etherification temperature, the amounts of reactants and the alkali boiling pretreatment which influence the carbo...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Yun T,Pang B,Lu J,Lv Y,Cheng Y,Wang H

    更新日期:2019-05-01 00:00:00

  • Alginate-based hydrogels for cancer therapy and research.

    abstract::Cancer is a major health issue concerning to all of us. Current treatment options are still limited due to not-selective action. Encapsulation is contemplated as an innovative approach to address systemic toxicity and tumor resistance caused by traditional therapies, while increasing encapsulated compounds bioavailabi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Reig-Vano B,Tylkowski B,Montané X,Giamberini M

    更新日期:2021-02-15 00:00:00

  • Magnetic field-driven drug release from modified iron oxide-integrated polysaccharide hydrogel.

    abstract::Salecan is a novel water-soluble extracellular β-glucan and suitable for the hydrogel preparation due to its excellent physicochemical and biological properties. The present article describes the fabrication and characterization of a pH/magnetic field-driven hydrogel based on salecan-g-poly(vinylacetic acid-co-2-hydro...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hu X,Wang Y,Zhang L,Xu M,Zhang J,Dong W

    更新日期:2018-03-01 00:00:00

  • Cell-specific and pH-sensitive nanostructure hydrogel based on chitosan as a photosensitizer carrier for selective photodynamic therapy.

    abstract::The major problems of porphyrins as promising materials for photodynamic therapy (PDT) are their low solubility, subsequently aggregation in biological environments, and a lack of tumor selectivity. With this in mind, a chitosan-based hydrogel conjugated with tetrakis(4-aminophenyl)porphyrin (NH2-TPP) and 2,4,6-tris(p...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Belali S,Karimi AR,Hadizadeh M

    更新日期:2018-04-15 00:00:00

  • Cellular imaging and folate receptor targeting delivery of gum kondagogu capped gold nanoparticles in cancer cells.

    abstract::In this study, the green synthesis of gum kondagogu capped gold nanoparticles (GK-GNPs) was prepared using a naturally available polysaccharide. The anionic gum capped GK-GNPs enabled the successful coupling of folic acid (FA) and fluorescein isothiocyanate (FITC) to produce a fluorescently labelled GNP (F2-GNP). F2-G...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Kumar SSD,Mahesh A,Antoniraj MG,Rathore HS,Houreld NN,Kandasamy R

    更新日期:2018-04-01 00:00:00

  • Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus.

    abstract::Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent st...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Sun YG,Li R,Jiang L,Qiao S,Zhi Y,Chen XX,Xie S,Wu J,Li X,Deng R,Zhang G

    更新日期:2018-10-01 00:00:00

  • A cutting-edge immunoinformatics approach for design of multi-epitope oral vaccine against dreadful human malaria.

    abstract::Human malaria is a pathogenic disease mainly caused by Plasmodium falciparum, which was responsible for about 405,000 deaths globally in the year 2018. To date, several vaccine candidates have been evaluated for prevention, which failed to produce optimal output at various preclinical/clinical stages. This study is ba...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Pritam M,Singh G,Swaroop S,Singh AK,Pandey B,Singh SP

    更新日期:2020-09-01 00:00:00

  • Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment.

    abstract::Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl alcohol) (PVA)/chito...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Bakhsheshi-Rad HR,Ismail AF,Aziz M,Akbari M,Hadisi Z,Omidi M,Chen X

    更新日期:2020-04-15 00:00:00

  • Crystal structure of prephenate dehydrogenase from Streptococcus mutans.

    abstract::Prephenate dehydrogenase (PDH) is a bacterial enzyme that catalyzes conversion of prephenate to 4-hydroxyphenylpyruvate through the oxidative decarboxylation pathway for tyrosine biosynthesis. This enzymatic pathway exists in prokaryotes but is absent in mammals, indicating that it is a potential target for the develo...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ku HK,Do NH,Song JS,Choi S,Yeon SH,Shin MH,Kim KJ,Park SR,Park IY,Kim SK,Lee SJ

    更新日期:2011-11-01 00:00:00

  • Characterization of Camptotheca acuminata 10-hydroxygeraniol oxidoreductase and iridoid synthase and their application in biological preparation of nepetalactol in Escherichia coli featuring NADP+ - NADPH cofactors recycling.

    abstract::Nepetalactol, an iridoid with four chiral carbons, is a crucial component of aphid sex pheromones that have been employed with great success to control the insect-related diseases. Despite of agricultural usage as end products, iridoids are fundamental biosynthetic intermediates for pharmaceutically important monoterp...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Awadasseid A,Li W,Liu Z,Qiao C,Pang J,Zhang G,Luo Y

    更新日期:2020-11-01 00:00:00

  • Electrospun chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering.

    abstract::This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan-graft-poly (ɛ-caprolactone) (CS-g-PCL) was synthesized with a facile one-step ma...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chen H,Huang J,Yu J,Liu S,Gu P

    更新日期:2011-01-01 00:00:00

  • Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples.

    abstract::Under favorable growth conditions, Staphylococcus aureus infects and causes illnesses in humans and animals. Staphylococcal enterotoxins are considered the most important factor for food poisoning, with these toxins usually found in milk and its byproducts. This study investigated the presence of a classical enterotox...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Nouri A,Ahari H,Shahbazzadeh D

    更新日期:2018-02-01 00:00:00

  • Optimized extraction of polysaccharides from Cymbopogon citratus and its biological activities.

    abstract::In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Thangam R,Suresh V,Kannan S

    更新日期:2014-04-01 00:00:00