Abstract:
:The structure of a ubiquitin-like protein, small ubiquitin-related modifier-1 (SUMO-1), was earlier determined using homonuclear nuclear magnetic resonance (NMR) spectroscopy, since the spectral quality of the protein was not suitable for heteronuclear NMR data collection. In this study, a slightly different construct of the SUMO-1 gene was used for protein over-expression. The protein purified from this construct showed high spectral qualities, therefore, multi-dimensional heteronuclear NMR data for a dynamic study and structural determination were acquired. The structure of SUMO-1 obtained in this study differs in several respects from the structure obtained from homonuclear NMR data. Furthermore, structural differences were observed between the new SUMO-1 and ubiquitin structures. These differences may be important for SUMO-1-specific recognition in cells. Additionally, relaxation parameters indicate that SUMO-1 undergoes highly anisotropic tumbling in solution and that the long amino (N)-terminal sequence of SUMO-1 is highly dynamic with increasing flexibility towards the end.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Jin C,Shiyanova T,Shen Z,Liao Xdoi
10.1016/s0141-8130(00)00169-0keywords:
subject
Has Abstractpub_date
2001-03-14 00:00:00pages
227-34issue
3eissn
0141-8130issn
1879-0003pii
S0141-8130(00)00169-0journal_volume
28pub_type
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