Applications and Approaches for Three-Dimensional Precision-Cut Lung Slices. Disease Modeling and Drug Discovery.

abstract：:Gene expression for tropoelastin, the proprotein for elastin, was examined in the rat lung from 17 days of gestation (pseudoglandular stage) to adulthood by in situ hybridization using a rat-specific 35S-radiolabeled riboprobe. The tropoelastin message was present in vascular and airway smooth muscle, endothelial, sep...

journal_title：American journal of respiratory cell and molecular biology

authors： Noguchi A,Samaha H

更新日期：1991-12-01 00:00:00

abstract：:The human lung is constantly exposed to the environment and potential pathogens. As the interface between host and environment, the respiratory epithelium has evolved sophisticated sensing mechanisms as part of its defense against pathogens. In this review, we examine how the respiratory epithelium senses and responds...

journal_title：American journal of respiratory cell and molecular biology

authors： Benam KH,Denney L,Ho LP

更新日期：2019-03-01 00:00:00

abstract：:Bacterial pneumonia is a common public health problem associated with significant mortality, morbidity, and cost. Neutrophils are usually the earliest leukocytes to respond to bacteria in the lungs. Neutrophils rapidly sequester in the pulmonary microvasculature and migrate into the lung parenchyma and alveolar spaces...

journal_title：American journal of respiratory cell and molecular biology

authors： Gomez JC,Yamada M,Martin JR,Dang H,Brickey WJ,Bergmeier W,Dinauer MC,Doerschuk CM

更新日期：2015-03-01 00:00:00

abstract：:It is generally important to elucidate airway epithelial cell lineages and to identify multipotent progenitors as targets for gene therapy. Stem (S) cells are typically present in specialized compartments spatially proximal to their differentiated progeny, but an equivalent paradigm has not been demonstrated in the ai...

journal_title：American journal of respiratory cell and molecular biology

authors： Borthwick DW,Shahbazian M,Krantz QT,Dorin JR,Randell SH

更新日期：2001-06-01 00:00:00

abstract：:Endotoxin (lipopolysaccharide [LPS]) is known to induce the production of tumor necrosis factor (TNF)-alpha and the induction of manganese superoxide dismutase (MnSOD). We have recently demonstrated that induction of TNF-alpha and MnSOD by LPS is mediated through different signal transduction pathways. In the current ...

journal_title：American journal of respiratory cell and molecular biology

authors： White JE,Tsan MF

更新日期：2001-02-01 00:00:00

abstract：:The nose is an attractive source of airway epithelial cells, particularly in populations in which bronchoscopy may not be possible. However, substituting nasal cells for bronchial epithelial cells in the study of airway inflammation depends upon comparability of responses, and evidence for this is lacking. Our objecti...

journal_title：American journal of respiratory cell and molecular biology

authors： McDougall CM,Blaylock MG,Douglas JG,Brooker RJ,Helms PJ,Walsh GM

更新日期：2008-11-01 00:00:00

abstract：:Long-term success in lung transplantation is limited by obliterative bronchiolitis, whereas T cell effector mechanisms in this process remain incompletely understood. Using the mouse heterotopic allogeneic airway transplant model, we studied T cell effector responses during obliterative airways disease (OAD). Allospec...

journal_title：American journal of respiratory cell and molecular biology

authors： West EE,Lavoie TL,Orens JB,Chen ES,Ye SQ,Finkelman FD,Garcia JG,McDyer JF

更新日期：2006-01-01 00:00:00

abstract：:Chronic obstructive pulmonary disease (COPD) has been associated with heterozygosity for the Z and S alleles of the alpha1-antitrypsin gene in some studies, but these observations have not been confirmed by others. Cigarette smoking is the major risk factor for COPD and may have been a confounding factor in many of th...

journal_title：American journal of respiratory cell and molecular biology

authors： Sandford AJ,Weir TD,Spinelli JJ,Paré PD

更新日期：1999-02-01 00:00:00

abstract：:Human tracheal gland serous (HTGS) cells are now considered one principal pulmonary target for the gene therapy of cystic fibrosis (CF). We developed a CF tracheal gland serous cell line, CF-KM4, obtained by the transformation of primary cultures of CF tracheal gland serous cells homozygous for the DeltaF508 mutation ...

journal_title：American journal of respiratory cell and molecular biology

authors： Kammouni W,Moreau B,Becq F,Saleh A,Pavirani A,Figarella C,Merten MD

更新日期：1999-04-01 00:00:00

abstract：:Recent studies have indicated that, during the development of pulmonary hypertension (PH), there is a switch from oxidative phosphorylation to glycolysis in the pulmonary endothelium. However, the mechanisms underlying this phenomenon have not been elucidated. Endothelin (ET)-1, an endothelial-derived vasoconstrictor ...

journal_title：American journal of respiratory cell and molecular biology

authors： Sun X,Kumar S,Sharma S,Aggarwal S,Lu Q,Gross C,Rafikova O,Lee SG,Dasarathy S,Hou Y,Meadows ML,Han W,Su Y,Fineman JR,Black SM

更新日期：2014-06-01 00:00:00

abstract：:Severe deterioration of surfactant function is noted under conditions of plasma protein leakage into the alveolar space; moreover, fibrinogen has previously been reported to possess strong surfactant inhibitory capacity. Dissolution of alveolar deposits of fibrinogen and fibrin (e.g., hyaline membranes) requires enzym...

journal_title：American journal of respiratory cell and molecular biology

authors： Seeger W,Grube C,Günther A

更新日期：1993-09-01 00:00:00

abstract：:Previous observations by our laboratory indicate that the presence of anti-IL-8 autoantibody:IL-8 immune complexes in lung fluids from patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS) comprises an important prognostic indicator in the development and ultimate outcome of ALI/ARDS. We also ...

journal_title：American journal of respiratory cell and molecular biology

authors： Krupa A,Fudala R,Florence JM,Tucker T,Allen TC,Standiford TJ,Luchowski R,Fol M,Rahman M,Gryczynski Z,Gryczynski I,Kurdowska AK

更新日期：2013-02-01 00:00:00

abstract：:Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution o...

journal_title：American journal of respiratory cell and molecular biology

authors： Morales-Nebreda LI,Rogel MR,Eisenberg JL,Hamill KJ,Soberanes S,Nigdelioglu R,Chi M,Cho T,Radigan KA,Ridge KM,Misharin AV,Woychek A,Hopkinson S,Perlman H,Mutlu GM,Pardo A,Selman M,Jones JC,Budinger GR

更新日期：2015-04-01 00:00:00

abstract：:Alveolar type (AT)I and ATII cells are central to maintaining normal alveolar fluid homeostasis. When disrupted, they contribute to the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome. Research on ATII cells has been limited by the inability to propagate primary cells in vitro to study ...

journal_title：American journal of respiratory cell and molecular biology

authors： Bove PF,Dang H,Cheluvaraju C,Jones LC,Liu X,O'Neal WK,Randell SH,Schlegel R,Boucher RC

更新日期：2014-04-01 00:00:00

abstract：:Although neuroanatomical and neurophysiological features of neurons in the ferret trachea have been studied, the neural mediators associated with this plexus have not been completely characterized. The purpose of this study was to examine the occurrence of choline acetyltransferase (ChAT), nitric oxide synthase (NOS),...

journal_title：American journal of respiratory cell and molecular biology

authors： Dey RD,Altemus JB,Rodd A,Mayer B,Said SI,Coburn RF

更新日期：1996-03-01 00:00:00

abstract：:Polymorphonuclear leukocytes (PMN) release gelatinase B in response to variable stimuli. Gelatinase B degrades basement membrane components in vitro, and inhibition of matrix metalloproteinase activity blunts PMN migration through a prototype basement membrane (Matrigel) and amnionic membranes. Accordingly, it has bee...

journal_title：American journal of respiratory cell and molecular biology

authors： Betsuyaku T,Shipley JM,Liu Z,Senior RM

更新日期：1999-06-01 00:00:00

abstract：:We demonstrated previously that FoxD1-derived cells in the lung are enriched in pericyte-like cells in mouse lung. These cells express the common pericyte markers and are located adjacent to endothelial cells. In this study, we demonstrate the feasibility of administering diphtheria toxin (DT) by oropharyngeal aspirat...

journal_title：American journal of respiratory cell and molecular biology

authors： Hung CF,Chow YH,Liles WC,Altemeier WA,Schnapp LM

更新日期：2017-02-01 00:00:00

abstract：:The glucocorticoids (GC) and retinoids (RA) modulate branching morphogenesis and cytodifferentiation in the developing lung. We investigated downstream target genes that link glucocorticoid stimulation to the achievement of a mature lung in glucocorticoid receptor (GR) knockout mice. All GR(null) mice and approximatel...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaplan F,Comber J,Sladek R,Hudson TJ,Muglia LJ,Macrae T,Gagnon S,Asada M,Brewer JA,Sweezey NB

更新日期：2003-01-01 00:00:00

abstract：:Opiate-like peptides can regulate many cellular functions. We now map [D-Ala(2)]deltorphin I (DADTI)-like immunoreactivity (DADTI-LI) in developing mouse lung and analyze potential functional roles. Most DADTI-LI-positive cells were alveolar cells negative for prosurfactant protein (proSP)-C immunoreactivity. Peak num...

journal_title：American journal of respiratory cell and molecular biology

authors： Sunday ME,Haley KJ,Emanuel RL,Torday JS,Asokananthan N,Sikorski KA,Tooyama I,Kimura H,Renda T,Erspamer V

更新日期：2001-10-01 00:00:00

abstract：:To examine the effects of acid exposure with moderate acidity (pH 3.0-5.0) on bactericidal activity of airway surface liquid (ASL), ASL was collected by washing the surface of primary cultures of human tracheal epithelial cells 24 h after treatment with phosphate-buffered saline (PBS) adjusted to a pH of 3.0, 4.0, or ...

journal_title：American journal of respiratory cell and molecular biology

authors： Nakayama K,Jia YX,Hirai H,Shinkawa M,Yamaya M,Sekizawa K,Sasaki H

更新日期：2002-01-01 00:00:00

abstract：:The hydroxyl radical (.OH) is a highly reactive oxygen free radical that has been implicated as a cause of lung injury following exposure to silica and silicates. Despite evidence that silica generates .OH in vitro, there has been no previous demonstration of in vivo production of .OH after exposure to nonfibrous mine...

journal_title：American journal of respiratory cell and molecular biology

authors： Schapira RM,Ghio AJ,Effros RM,Morrisey J,Almagro UA,Dawson CA,Hacker AD

更新日期：1995-02-01 00:00:00

abstract：:Lung development is associated with a surge in surfactant phosphatidylcholine (PC) production to prepare the newborn for extrauterine breathing. This process is associated with a marked increase in the activity of the rate-regulatory surfactant enzyme, CTP:phosphocholine cytidylyltransferase (CCTalpha). To investigate...

journal_title：American journal of respiratory cell and molecular biology

authors： McCoy DM,Fisher K,Robichaud J,Ryan AJ,Mallampalli RK

更新日期：2006-09-01 00:00:00

abstract：:The differentiated phenotype of the alveolar type II cell is rapidly altered in vitro. To evaluate factors that might influence this process, we isolated and plated rat type II cells in serum-supplemented media to promote adherence and then maintained the cells in a simple nutrient medium in the absence (S- cells) or ...

journal_title：American journal of respiratory cell and molecular biology

authors： Paine R 3rd,Joyce-Brady M,Clement A,Brody JS

更新日期：1990-10-01 00:00:00

abstract：:Bronchiolitis obliterans (BO) is an increasingly important lung disease characterized by fibroproliferative airway lesions and decrements in lung function. Occupational exposure to the artificial food flavoring ingredient diacetyl, commonly used to impart a buttery flavor to microwave popcorn, has been associated with...

journal_title：American journal of respiratory cell and molecular biology

authors： Brass DM,Gwinn WM,Valente AM,Kelly FL,Brinkley CD,Nagler AE,Moseley MA,Morgan DL,Palmer SM,Foster MW

更新日期：2017-06-01 00:00:00

abstract：:Cytolytic lymphocytes play an important role in defense against viral and neoplastic disease. Integral to the function of these cells is the content of lysosomal granules. Recent attention has focused on a family of proteases present in the granules of natural killer (NK) cells, interleukin-2 (IL-2)-activated NK cells...

journal_title：American journal of respiratory cell and molecular biology

authors： Breite WM,Dal Nogare AR,Yarbrough WC Jr,Weissler JC

更新日期：1990-12-01 00:00:00

abstract：:Integrins are heterodimeric cell-surface glycoproteins that mediate cell-cell and cell-matrix adhesion. We previously identified cDNA encoding a novel integrin beta subunit, beta 7, from bronchoalveolar lavage fluid (BALF) leukocytes. The beta 7 subunit protein is now known to associate with at least two integrin alph...

journal_title：American journal of respiratory cell and molecular biology

authors： Erle DJ,Brown T,Christian D,Aris R

更新日期：1994-03-01 00:00:00

abstract：:The vascular endothelium plays an important role in the regulation of inflammatory responses after trauma and hemorrhage. Interactions of neutrophils with endothelial cells (ECs) contribute to the activation of specific EC responses involved in innate immunity. We have previously reported that oxidants derived from th...

journal_title：American journal of respiratory cell and molecular biology

authors： Xiang M,Yin L,Li Y,Xiao G,Vodovotz Y,Billiar TR,Wilson MA,Fan J

更新日期：2011-03-01 00:00:00

abstract：:Lung transplantation remains the only therapeutic option for many patients suffering from end-stage pulmonary disease. Long-term success after lung transplantation is severely limited by the development of bronchiolitis obliterans. The murine heterotopic tracheal transplantation model has been widely used for studies ...

journal_title：American journal of respiratory cell and molecular biology

authors： Okazaki M,Gelman AE,Tietjens JR,Ibricevic A,Kornfeld CG,Huang HJ,Richardson SB,Lai J,Garbow JR,Patterson GA,Krupnick AS,Brody SL,Kreisel D

更新日期：2007-12-01 00:00:00

abstract：:To evaluate the response of an epithelial barrier to a moderate but sustained oxidative stress, we cultured monolayers of Madin Darby canine kidney cells on microporous filters and exposed them to the hypoxanthine-xanthine oxidase (HX-XO) reaction. The transepithelial permeability coefficient for mannitol (Pm) was ass...

journal_title：American journal of respiratory cell and molecular biology

authors： Rochat T,Burkhard C,Finci-Cerkez V,Meda P

更新日期：1993-11-01 00:00:00

abstract：:Cellular senescence has been implicated in diverse pathologies. However, there is conflicting evidence regarding the role of this process in tissue fibrosis. Although dysregulation of microRNAs is a key mechanism in the pathogenesis of lung fibrosis, it is unclear whether microRNAs function by regulating cellular sene...

journal_title：American journal of respiratory cell and molecular biology

authors： Cui H,Ge J,Xie N,Banerjee S,Zhou Y,Antony VB,Thannickal VJ,Liu G

更新日期：2017-02-01 00:00:00