Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development.

abstract：:Photosynthetic bacteria are capable of producing their own food via photosynthesis. Unsurprisingly, they evolved the ability to move toward better light conditions (i.e., phototaxis). In a recent article in mBio, Chau et al. tuned the wavelength, flux, direction, and timing of light input and characterized the motilit...

journal_title：mBio

authors： Kim M

更新日期：2017-04-11 00:00:00

abstract：:Increasing evidence has indicated that single nucleotide polymorphisms (SNPs) are related to the susceptibility of sepsis and might provide potential evidence for the mechanisms of sepsis. Our recent preliminary study showed that the ADAM10 genetic polymorphism was clinically associated with the development of sepsis,...

journal_title：mBio

authors： Chen F,Wang Y,Zhang W,Cai Y,Zhao T,Mai H,Tao S,Wei W,Li J,Chen X,Li X,Tang P,Fan W,Yang J,Ou M,Lu F,Lai Z,Chen H,Zou T,Sun F,Shao Y,Cui L

更新日期：2019-08-06 00:00:00

abstract：:The clonal population structure of Candida albicans suggests that (para)sexual recombination does not play an important role in the lifestyle of this opportunistic fungal pathogen, an assumption that is strengthened by the fact that most C. albicans strains are heterozygous at the mating type locus (MTL) and therefore...

journal_title：mBio

authors： Popp C,Ramírez-Zavala B,Schwanfelder S,Krüger I,Morschhäuser J

更新日期：2019-02-05 00:00:00

abstract：:This research analyzed six Aspergillus fumigatus genes encoding putative efflux proteins for their roles as transporters. TheA. fumigatus genes abcA, abcC, abcF, abcG, abcH, and abcI were cloned into plasmids and overexpressed in a Saccharomyces cerevisiae strain in which the highly active endogenous ABC transporter g...

journal_title：mBio

authors： Esquivel BD,Rybak JM,Barker KS,Fortwendel JR,Rogers PD,White TC

更新日期：2020-03-24 00:00:00

abstract：:The chemistry underpinning microbial interactions provides an integrative framework for linking the activities of individual microbes, microbial communities, plants, and their environments. Currently, we know very little about the functions of genes and metabolites within these communities because genome annotations a...

journal_title：mBio

authors： Zhalnina K,Zengler K,Newman D,Northen TR

更新日期：2018-07-17 00:00:00

abstract：UNLABELLED:Nap1 has long been identified as a potential septin regulator in yeasts. However, its function and regulation remain poorly defined. Here, we report functional characterization of Nap1 in the human-pathogenic fungus Candida albicans. We find that deletion of NAP1 causes constitutive filamentous growth and ch...

journal_title：mBio

authors： Huang ZX,Zhao P,Zeng GS,Wang YM,Sudbery I,Wang Y

更新日期：2014-02-04 00:00:00

abstract：:We showed previously that the histone lysine methyltransferase (HKMT) H3K27me3 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and is required for the maintenance of HIV-1 latency in Jurkat T cells. Here we show, by using chromatin immunoprecipitation experiments, that both PRC2 and euchromatic...

journal_title：mBio

authors： Nguyen K,Das B,Dobrowolski C,Karn J

更新日期：2017-02-28 00:00:00

abstract：UNLABELLED:Elevated intracellular levels of the bacterial second messenger c-di-GMP are known to suppress motility and promote sessility. Bacterial chemotaxis guides motile cells in gradients of attractants and repellents over broad concentration ranges, thus allowing bacteria to quickly adapt to changes in their surro...

journal_title：mBio

authors： Russell MH,Bible AN,Fang X,Gooding JR,Campagna SR,Gomelsky M,Alexandre G

更新日期：2013-03-19 00:00:00

abstract：:Bacteria of the phylum Bacteroidetes, including commensal organisms and opportunistic pathogens, harbor abundant surface-exposed multiprotein membrane complexes (Sus-like systems) involved in carbohydrate acquisition. These complexes have been mostly linked to commensalism, and in some instances, they have also been s...

journal_title：mBio

authors： Lauber F,Cornelis GR,Renzi F

更新日期：2016-10-25 00:00:00

abstract：:For decades, identifying the regions of a bacterial chromosome that are necessary for viability has relied on mapping integration sites in libraries of random transposon mutants to find loci that are unable to sustain insertion. To date, these studies have analyzed subsaturated libraries, necessitating the application...

journal_title：mBio

authors： DeJesus MA,Gerrick ER,Xu W,Park SW,Long JE,Boutte CC,Rubin EJ,Schnappinger D,Ehrt S,Fortune SM,Sassetti CM,Ioerger TR

更新日期：2017-01-17 00:00:00

abstract：:The proteasome is a major protein degradation machinery with essential and diverse biological functions. Upon induction by cytokines, proteasome subunits β1, β2, and β5 are replaced by β1i/LMP2, β2i/MECL-1, and β5i/LMP7, resulting in the formation of an immunoproteasome (iProteasome). iProteasome-degraded products are...

journal_title：mBio

authors： Yang Y,Liu W,Hu D,Su R,Ji M,Huang Y,Shereen MA,Xu X,Luo Z,Zhang Q,Liu F,Wu K,Liu Y,Wu J

更新日期：2020-10-27 00:00:00

abstract：UNLABELLED:β-Lactam antibiotics are the drugs of choice to treat pneumococcal infections. The spread of β-lactam-resistant pneumococci is a major concern in choosing an effective therapy for patients. Systematically tracking β-lactam resistance could benefit disease surveillance. Here we developed a classification syst...

journal_title：mBio

authors： Li Y,Metcalf BJ,Chochua S,Li Z,Gertz RE Jr,Walker H,Hawkins PA,Tran T,Whitney CG,McGee L,Beall BW

更新日期：2016-06-14 00:00:00

abstract：:UDP-N-acetylglucosamine (UDP-GlcNAc), the main product of the hexosamine biosynthetic pathway, is an important metabolite in protozoan parasites since its sugar moiety is incorporated into glycosylphosphatidylinositol (GPI) glycolipids and N- and O-linked glycans. Apicomplexan parasites have a hexosamine pathway compa...

journal_title：mBio

authors： Chi J,Cova M,de Las Rivas M,Medina A,Borges RJ,Leivar P,Planas A,Usón I,Hurtado-Guerrero R,Izquierdo L

更新日期：2020-10-20 00:00:00

abstract：UNLABELLED:DNA lesions in the template strand block synthesis by replicative DNA polymerases (Pols). Eukaryotic cells possess a number of specialized translesion synthesis (TLS) Pols with the ability to replicate through DNA lesions. The Epstein-Barr virus (EBV), a member of the herpesvirus family, infects human B cell...

journal_title：mBio

authors： Yoon JH,Prakash S,Prakash L

更新日期：2012-09-11 00:00:00

abstract：UNLABELLED:Exploiting mechanisms of utilizing the sugar d-galactose in Escherichia coli as a model system, we explored the consequences of accumulation of critical intermediates of the d-galactose metabolic pathways by monitoring cell growth, metabolites, and transcript profiles. These studies revealed both metabolic n...

journal_title：mBio

authors： Lee SJ,Trostel A,Adhya S

更新日期：2014-01-28 00:00:00

abstract：:In Escherichia coli, FtsEX coordinates peptidoglycan (PG) synthesis and hydrolysis at the septum. It acts on FtsA in the cytoplasm to promote recruitment of septal PG synthetases and recruits EnvC, an activator of septal PG hydrolases, in the periplasm. Following recruitment, ATP hydrolysis by FtsEX is thought to regu...

journal_title：mBio

authors： Du S,Pichoff S,Lutkenhaus J

更新日期：2020-07-07 00:00:00

abstract：:Nutritional immunity is an elegant host mechanism used to starve invading pathogens of necessary nutrient metals. Calprotectin, a metal-binding protein, is produced abundantly by neutrophils and is found in high concentrations within inflammatory sites during infection. Group B Streptococcus (GBS) colonizes the gastro...

journal_title：mBio

authors： Burcham LR,Le Breton Y,Radin JN,Spencer BL,Deng L,Hiron A,Ransom MR,Mendonça JDC,Belew AT,El-Sayed NM,McIver KS,Kehl-Fie TE,Doran KS

更新日期：2020-11-10 00:00:00

abstract：:Low doses of antibiotics can trigger secondary metabolite biosynthesis in bacteria, but the underlying mechanisms are generally unknown. We sought to better understand this phenomenon by studying how the antibiotic trimethoprim activates the synthesis of the virulence factor malleilactone in Burkholderia thailandensis...

journal_title：mBio

authors： Li A,Mao D,Yoshimura A,Rosen PC,Martin WL,Gallant É,Wühr M,Seyedsayamdost MR

更新日期：2020-02-25 00:00:00

abstract：UNLABELLED:Promiscuous plasmids replicate in a wide range of bacteria and therefore play a key role in the dissemination of various host-beneficial traits, including antibiotic resistance. Despite the medical relevance, little is known about the evolutionary dynamics through which drug resistance plasmids adapt to new ...

journal_title：mBio

authors： Hughes JM,Lohman BK,Deckert GE,Nichols EP,Settles M,Abdo Z,Top EM

更新日期：2012-07-03 00:00:00

abstract：:Motility is an important virulence trait for many bacterial pathogens, allowing them to position themselves in appropriate locations at appropriate times. The motility structures type IV pili and flagella are also involved in sensing surface contact, which modulates pathogenicity. In Pseudomonas aeruginosa, the PilS-P...

journal_title：mBio

authors： Kilmury SLN,Burrows LL

更新日期：2018-07-24 00:00:00

abstract：:Malaria parasites increase their host erythrocyte's permeability to various nutrients, fueling intracellular pathogen development and replication. The plasmodial surface anion channel (PSAC) mediates this uptake and is linked to the parasite-encoded RhopH complex, consisting of CLAG3, RhopH2, and RhopH3. While interac...

journal_title：mBio

authors： Ahmad M,Manzella-Lapeira J,Saggu G,Ito D,Brzostowski JA,Desai SA

更新日期：2020-09-08 00:00:00

abstract：UNLABELLED:The bacterial flagellum is driven by a bidirectional rotary motor, which propels bacteria to swim through liquids or swarm over surfaces. While the functions of the major structural and regulatory components of the flagellum are known, the function of the well-conserved FliL protein is not. In Salmonella and...

journal_title：mBio

authors： Partridge JD,Nieto V,Harshey RM

更新日期：2015-02-24 00:00:00

abstract：UNLABELLED:The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) causes a severe respiratory infection with a high mortality rate (~35%). MERS-CoV has been a global threat due to continuous outbreaks in the Arabian peninsula and international spread by infected travelers since 2012. From May to Jul...

journal_title：mBio

authors： Kim Y,Cheon S,Min CK,Sohn KM,Kang YJ,Cha YJ,Kang JI,Han SK,Ha NY,Kim G,Aigerim A,Shin HM,Choi MS,Kim S,Cho HS,Kim YS,Cho NH

更新日期：2016-03-01 00:00:00

abstract：:Avian influenza viruses (AIVs) must acquire mammalian-adaptive mutations before they can efficiently replicate in and transmit among humans. The PB2 E627K mutation is known to play a prominent role in the mammalian adaptation of AIVs. The H7N9 AIVs that emerged in 2013 in China easily acquired the PB2 E627K mutation u...

journal_title：mBio

authors： Liang L,Jiang L,Li J,Zhao Q,Wang J,He X,Huang S,Wang Q,Zhao Y,Wang G,Sun N,Deng G,Shi J,Tian G,Zeng X,Jiang Y,Liu L,Liu J,Chen P,Bu Z,Kawaoka Y,Chen H,Li C

更新日期：2019-06-18 00:00:00

abstract：UNLABELLED:The Pseudomonas aeruginosa type III secretion system has been associated with poor outcomes in both animal models and human patients. Despite a large number of studies exploring the regulation of type III secretion in vitro, little is known about the timing of secretion during mammalian infection. Here we de...

journal_title：mBio

authors： Howell HA,Logan LK,Hauser AR

更新日期：2013-03-12 00:00:00

abstract：:Distinct mammalian RNA viruses trigger Dicer-mediated production of virus-derived small-interfering RNAs (vsiRNA) and encode unrelated proteins to suppress vsiRNA biogenesis. However, the mechanism and function of the mammalian RNA interference (RNAi) response are poorly understood. Here, we characterized antiviral RN...

journal_title：mBio

authors： Han Q,Chen G,Wang J,Jee D,Li WX,Lai EC,Ding SW

更新日期：2020-08-04 00:00:00

abstract：UNLABELLED:Glycoside hydrolases (GHs) are key enzymes in the depolymerization of plant-derived cellulose, a process central to the global carbon cycle and the conversion of plant biomass to fuels and chemicals. A limited number of GH families hydrolyze crystalline cellulose, often by a processive mechanism along the ce...

journal_title：mBio

authors： Hiras J,Wu YW,Deng K,Nicora CD,Aldrich JT,Frey D,Kolinko S,Robinson EW,Jacobs JM,Adams PD,Northen TR,Simmons BA,Singer SW

更新日期：2016-08-23 00:00:00

abstract：:Protein ubiquitylation regulates not only endocellular trafficking and proteasomal degradation but also the catalytic activity of enzymes. In Saccharomyces cerevisiae, we analyzed the composition of the ubiquitylated proteomes in strains lacking acetyltransferase Gcn5p, Ub-protease Ubp8p, or both to understand their i...

journal_title：mBio

authors： De Palma A,Fanelli G,Cretella E,De Luca V,Palladino RA,Panzeri V,Roffia V,Saliola M,Mauri P,Filetici P

更新日期：2020-08-11 00:00:00

abstract：:A defining activity of retroviruses is reverse transcription, the process by which the viral genomic RNA is converted into the double-stranded DNA required for virus replication. Reverse transcriptase (RT), the viral enzyme responsible for this process, was identified in 1970 by assaying permeabilized retrovirus parti...

journal_title：mBio

authors： Jennings J,Shi J,Varadarajan J,Jamieson PJ,Aiken C

更新日期：2020-12-01 00:00:00

abstract：:The concept that anaerobic microorganisms can directly accept electrons from Fe(0) has been controversial because direct metal-microbe electron transfer has previously only been indirectly inferred. Fe(0) oxidation was studied with Geobacter sulfurreducens strain ACL, an autotrophic strain that was previously shown to...

journal_title：mBio

authors： Tang HY,Holmes DE,Ueki T,Palacios PA,Lovley DR

更新日期：2019-05-14 00:00:00