Abstract:
:Escherichia coli and Salmonella Enteritidis are foodborne pathogens forming challenging biofilms that contribute to their virulence, antimicrobial resistance, and survival on surfaces. Interspecies interactions occur between species in mixed biofilms promoting different outcomes to each species. Here we describe the interactions between E. coli and S. Enteritidis strains, and their control using specific phages. Single-species biofilms presented more cells compared to dual-species biofilms. The spatial organization of strains, observed by confocal microscopy, revealed similar arrangements in both single- and dual-species biofilms. The EPS matrix composition, assessed by Fourier-transform infrared spectroscopy, disclosed that the spectra extracted from the different dual-species biofilms can either be a combination of both species EPS matrix components or that the EPS matrix of one species predominates. Phages damaged more the single-species biofilms than the mixed biofilms, showing also that the killing efficacy was greatly dependent on the phage growth characteristics, bacterial growth parameters, and bacterial spatial distribution in biofilms. This combination of methodologies provides new knowledge of species-species and phage-host interactions in biofilms of these two major foodborne pathogens.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Milho C,Silva MD,Alves D,Oliveira H,Sousa C,Pastrana LM,Azeredo J,Sillankorva Sdoi
10.1038/s41598-019-54847-ysubject
Has Abstractpub_date
2019-12-03 00:00:00pages
18183issue
1issn
2045-2322pii
10.1038/s41598-019-54847-yjournal_volume
9pub_type
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