Abstract：
:Sodium taurocholate cotransporting polypeptide (NTCP) is a recently discovered hepatitis B virus (HBV) receptor. In the present study, we used TK-NOG mice with a humanized liver to examine the impact of endogenous NTCP expression on HBV infection. Upon inoculation with HBV, these mice exhibited clear viremia in 2 weeks, and serum HBV DNA levels gradually increased. The frequency of HBsAg-positive hepatocytes in the liver was 5.1 ± 0.6% at 2 weeks and increased with increasing HBV DNA levels, reaching 92.9 ± 2.8% at 10 to 12 weeks. In vivo siRNA-mediated NTCP knockdown before and after HBV inoculation significantly suppressed the levels of HBV replication and the frequency of HBsAg-positive hepatocytes at 2 weeks, whereas NTCP knockdown 13 weeks after infection did not affect these parameters. Similar to the humanized mouse livers in the early phase of HBV infection, human liver samples from chronic hepatitis B patients, especially those treated with nucleos(t)ide analogues, contained a considerable number of hepatocytes that were negative for the anti-HBs antibody. In conclusion, NTCP inhibition prevents the spread of HBV-infected hepatocytes in mice with a humanized liver. NTCP-targeted therapy has potential for regulating HBV infection in patients with chronic hepatitis B.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nakabori T,Hikita H,Murai K,Nozaki Y,Kai Y,Makino Y,Saito Y,Tanaka S,Wada H,Eguchi H,Takahashi T,Suemizu H,Sakamori R,Hiramatsu N,Tatsumi T,Takehara Tdoi
10.1038/srep27782subject
Has Abstractpub_date
2016-06-09 00:00:00pages
27782issn
2045-2322pii
srep27782journal_volume
6pub_type
杂志文章abstract：:A Late Cretaceous-aged multi-taxon nesting site from Romania preserved in three dimensions reveals the earliest example of nest site sharing yet known from the vertebrate fossil record. Eggshell and osteological evidence combined in this single accumulation demonstrate that at least four vertebrate taxa including enan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36305-3
更新日期：2019-02-13 00:00:00
abstract：:Decoders optimized offline to reconstruct intended movements from neural recordings sometimes fail to achieve optimal performance online when they are used in closed-loop as part of an intracortical brain-computer interface (iBCI). This is because typical decoder calibration routines do not model the emergent interact...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44166-7
更新日期：2019-06-20 00:00:00
abstract：:Surgery and anesthesia induce inflammatory changes in the central nervous system, which ultimately lead to neuronal damage concomitant with an increase in the level of neurodegeneration markers. Despite some experimental data showing prolonged activation of the immune system post-surgery, no study has determined the e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42351-2
更新日期：2019-05-09 00:00:00
abstract：:During symbiosis, organisms use a range of metabolic and protein-based signals to communicate. Of these protein signals, one class is defined as 'effectors', i.e., small secreted proteins (SSPs) that cause phenotypical and physiological changes in another organism. To date, protein-based effectors have been described ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00400-8
更新日期：2017-03-23 00:00:00
abstract：:Discriminating between inherited and non-inherited sporadic hearing loss is challenging. Here, we attempted to delineate genetic inheritance in simplex cases of severe-to-profound congenital hearing loss in Korean children. Variations in SLC26A4 and GJB2 in 28 children with bilateral severe-to-profound non-syndromic h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45973
更新日期：2017-04-06 00:00:00
abstract：:Nasoseptal cartilage has been assumed to be isotropic, unlike the well-defined zonal organization of articular cartilage attributed to postnatal biomechanical loading. We know from clinical experience that malrotation of surgical nasoseptal cartilage grafts can lead to increased graft absorption. Other studies have al...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48578-3
更新日期：2019-08-28 00:00:00
abstract：:Chinese sturgeon (Acipenser sinensis) has been listed as a critically endangered species on the IUCN Red List and is an endemic fish of China. Five sets of duplex polymerase chain reactions (PCR) assays were developed with 10 tetranucleotide microsatellites for Chinese sturgeon. The size of CS57, ZHX43, ZHX69, AS105, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60401-y
更新日期：2020-02-26 00:00:00
abstract：:Across the planet the biogeographic distribution of human cultural diversity tends to correlate positively with biodiversity. In this paper we focus on the biogeographic distribution of mammal species and human cultural diversity. We show that not only are these forms of diversity similarly distributed in space, but t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76658-2
更新日期：2020-11-12 00:00:00
abstract：:A frequency reconfigurable dipole antenna based on a silicon radiator is presented. The silicon radiator is activated with the aid of highly dense solid-state plasma by injecting carriers into the intrinsic region of p-i-n diodes. The fabrication and design guideline of the reconfigurable dipole antenna with this plas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33278-1
更新日期：2018-10-09 00:00:00
abstract：:The degree of tumor removal at surgery is a major factor in predicting outcome for ovarian cancer. A single multimodality agent that can be used with magnetic resonance (MR) for staging and pre-surgical planning, and with optical imaging to aid surgical removal of tumors, would present a new paradigm for ovarian cance...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38991
更新日期：2016-12-22 00:00:00
abstract：:Heavy metal pollution has been a problem since the advent of modern transportation, which despite efforts to curb emissions, continues to play a critical role in environmental pollution. Copper ions (Cu2+), in particular, are one of the more prevalent metals that have widespread detrimental ramifications. From this pe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35565
更新日期：2016-10-18 00:00:00
abstract：:Leptin signaling in the hypothalamus plays a crucial role in the regulation of body weight. Leptin resistance, in which leptin signaling is disrupted, is a major obstacle to the improvement of obesity. We herein demonstrated that protein tyrosine phosphatase receptor type J (Ptprj) is expressed in hypothalamic neurons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12070-7
更新日期：2017-09-14 00:00:00
abstract：:The role of miRNAs in mediating insecticide resistance remains largely unknown, even for the model species Drosophila melanogaster. Building on prior research, this study used microinjection of synthetic miR-310s mimics into DDT-resistant 91-R flies and observed both a significant transcriptional repression of computa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71250-0
更新日期：2020-09-01 00:00:00
abstract：:In the study, 15 K. pneumoniae strains were isolated from the mink experiencing respiratory distress in mideastern Shandong province, China, and the prevalence of K. pneumoniae in the sampled mink was 11.9% (15/126). Fourteen (93.33%) of the 15 K. pneumoniae isolates were identified as serotype K2 and hypermucoviscosi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17681-8
更新日期：2017-12-11 00:00:00
abstract：:Migraines are a common and undertreated disease and often have psychiatric comorbidities; however, the abnormal mechanism of emotional processing in migraine patients has not been well clarified. This study sought to investigate the different brain functional activation to neutral, positive and negative emotional stim...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41919
更新日期：2017-02-09 00:00:00
abstract：:Streptococcus anginosus is increasingly recognized as an opportunistic pathogen. However, our knowledge about virulence determinants in this species is scarce. One exception is the streptolysin-S (SLS) homologue responsible for the β-hemolytic phenotype of the S. anginosus type strain. In S. anginosus the expression o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27334-z
更新日期：2018-06-13 00:00:00
abstract：:The characteristics of aortic valvular outflow jet affect aortopathy in the bicuspid aortic valve (BAV). This study aimed to elucidate the effects of BAV morphology on the aortic valvular outflow jets. Morphotype-specific valve-devising apparatuses were developed to create aortic valve models. A magnetic resonance ima...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81845-w
更新日期：2021-01-22 00:00:00
abstract：:Wss1 is a DNA-protein crosslinks (DPCs) repair protein, which is responsible for degradation of the protein components in DPCs. In this investigation, crystal structure of the protease domain from saccharomyces cerevisiae Wss1 (ScWss1) was solved and was compared with the known crystal structure of Schizosaccharomyces...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08834-w
更新日期：2017-08-15 00:00:00
abstract：:Human respiratory syncytial virus (RSV) A ON1 genotype, first detected in 2010 in Ontario, Canada, has been documented in 21 countries to date. This study investigated persistence and transmission dynamics of ON1 by grouping 406 randomly selected RSV-positive specimens submitted to Public Health Ontario from August 20...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14268
更新日期：2015-09-30 00:00:00
abstract：:Linear dichroism - the polarization dependent absorption of electromagnetic waves- is routinely exploited in applications as diverse as structure determination of DNA or polarization filters in optical technologies. Here filamentary absorbers with a large length-to-width ratio are a prerequisite. For magnetization dyn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07020-2
更新日期：2017-08-01 00:00:00
abstract：:Multi-drug resistant efflux transporters found in Blood-Brain Barrier (BBB) acts as a functional barrier, by pumping out most of the drugs into the blood. Previous studies showed focused ultrasound (FUS) induced microbubble oscillation can disrupt the BBB by loosening the tight junctions in the brain endothelial cells...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31201
更新日期：2016-08-11 00:00:00
abstract：:Keratoconus is a common degenerative corneal disease that can lead to significant visual morbidity, and both genetic and environmental factors have been implicated in its pathogenesis. We compared the transcriptome of keratoconus and control epithelium using RNA-Seq. Epithelial tissues were obtained prior to surgery f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18480-x
更新日期：2018-01-10 00:00:00
abstract：:In this study, we reported a new technique based on laser speckle flowgraphy to record the ocular blood flow in rabbits under deep anesthesia, and proposed parameters to characterize retinal ischemia. We applied the proposed technique to study the correlation of blood flow between the eyes of normal non-anesthetized a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24141-4
更新日期：2018-04-09 00:00:00
abstract：:Rotator cuff tears are common and are associated with shoulder pain, disability, and dysfunction. Previous studies that have reported different arthroscopic techniques are controversial. A network meta-analysis with indirect and direct evidence was performed to compare the effectiveness of arthroscopic techniques for ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-019-40641-3
更新日期：2019-03-11 00:00:00
abstract：:Recent studies have elucidated the crucial role for microRNAs in peripheral nerve myelination by ablating components of the microRNA synthesis machinery. Few studies have focused on the role of individual microRNAs. To fill this gap, we focused this study on miR-138, which was shown to be drastically reduced in Dicer1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22010-8
更新日期：2018-02-28 00:00:00
abstract：:Endonuclease V (EndoV) is a ubiquitous protein present in all three kingdoms of life, responsible for the specific cleavages at the second phosphodiester bond 3' to inosine. E. coli EndoV (EcEndoV) is the first member discovered in the EndoV family. It is a small protein with a compact gene organization, yet with a wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12754
更新日期：2015-08-05 00:00:00
abstract：:Aberrant immune responses characterize autoimmune disorders like Rheumatoid Arthritis (RA) wherein lymphocytes are recognized as key players. Role of CD8+ T cells in RA has been less defined however we found that these cells are activated in RA patients with increased expression of cytolytic granules and inflammatory ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01033-7
更新日期：2017-04-19 00:00:00
abstract：:Tumour growth and metastatic colonization is strongly influenced by the tumour stroma, including cancer-associated fibroblasts (CAF). Multipotent mesenchymal stromal cells (MSC) are a possible source of CAF following myofibroblastic differentiation, and we have previously shown that MSC support tumour growth. Triggere...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48142-z
更新日期：2019-08-13 00:00:00
abstract：:Patients with asymptomatic occlusion in the internal carotid arteries (ICA) have been shown to have a better preserved hemodynamic status of the brain as compared to patients with symptoms. This study was aimed to explore the cerebral perfusion alterations in asymptomatic patients using multi-parametric arterial spin-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02094-4
更新日期：2017-05-12 00:00:00
abstract：:Tuned gene expression is crucial to the proper growth and response to the environmental changes of an organism. To enable tunable gene expression as designed is desirable in both scientific research and industrial application. Here, we introduce a novel promoter switching method based on the DDI2 promoter (PDDI2) that...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49000-8
更新日期：2019-08-29 00:00:00
