Abstract：
:AcMNPV is the first baculovirus to be sequenced and is considered a model of baculovirus. ac154 is a later expression gene in AcMNPV genome and its function is unknown. In this study, we explored the function of Ac154 in AcMNPV infection process in host Sf9 cells. The results showed that Ac154 was distributed in both nucleus and cytoplasm. Knockout of ac154 did not affect the production of BV, but the yield of progeny virus was reduced, indicating the auxiliary function of Ac154 in virus production. MTT assay showed that Ac154 promoted the proliferation and inhibited apoptosis of Sf9 cells. Overexpression of ac154 gene significantly increased the transcription level of anti-apoptotic gene p35, and delayed the expression of the pro-apoptotic protein SfP53 and reduced its expression level, which indicated its anti-apoptotic role in the host cells. In conclusion, our results demonstrated Ac154 could delay apoptosis process in host cells by regulating the transcription of p35 gene and the expression of SfP53 protein, which provided a more favorable environment for progeny virus replication and packaging, thereby promoting the proliferation of progeny virus. So we provided a potentially improved bac-to-bac eukaryotic protein expression system and biopesticide in this work.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Bai H,Liang A,Fu Ydoi
10.1007/s11010-019-03631-7subject
Has Abstractpub_date
2020-01-01 00:00:00pages
79-90issue
1-2eissn
0300-8177issn
1573-4919pii
10.1007/s11010-019-03631-7journal_volume
463pub_type
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journal_title：Molecular and cellular biochemistry
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