Abstract：
:Synaptic vesicle protein 2A (SV2A), which plays an important role in the pathophysiology of epilepsy, is a unique vesicular protein recognized as a pharmacological target of anticonvulsant drugs. Furthermore, SV2A is a potential synaptic density marker, as it is ubiquitously expressed throughout the brain in all nerve terminals independently of their neurotransmitter content. Due to the growing interest in this protein, we thoroughly analyzed SV2A levels, expression patterns and colocalization in both excitatory and inhibitory synapses among different brain structures in healthy rats. In addition, we discuss the main semiquantitative methodologies used to study SV2A because these techniques might represent powerful tools for evaluating synaptic changes associated with brain disorders. Our results showed that the SV2A expression levels differed among the analyzed structures, and a positive correlation between the SV2A mRNA copy number and protein level was observed by Western blot. In addition, immunohistochemistry demonstrated slight but consistent asymmetrical SV2A levels in different laminated structures, and SV2A expression was increased by up to 40% in some specific layers compared to that in others. Finally, triple immunofluorescence revealed strong SV2A colocalization with GABAergic terminals, mainly around the principal cells, suggesting that SV2A primarily participates in this inhibitory system in different rat brain structures. Although the SV2A protein is considered a good candidate marker of synaptic density, our data show that changes in its expression in pathological processes must be viewed as not only increased or decreased synapse numbers but also in light of the type of neurotransmission being affected.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Mendoza-Torreblanca JG,García-Cruz ME,Sánchez-Cruz I,Gomez-Gonzalez B,Juárez-Méndez S,Gómez-Lira Gdoi
10.1016/j.neuroscience.2019.09.004subject
Has Abstractpub_date
2019-11-01 00:00:00pages
108-120eissn
0306-4522issn
1873-7544pii
S0306-4522(19)30649-9journal_volume
419pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:The mechanism of neuropeptide secretion induced by bradykinin from capsaicin-sensitive afferents was studied in guinea-pig atria. Both the inotropic response induced by bradykinin (0.1 microM) in the electrically driven isolated guinea-pig left atria and the bradykinin (10 microM)-induced release of calcitonin gene-re...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(90)90062-9
更新日期：1990-01-01 00:00:00
abstract：:High resolution immunogold cytochemistry was used to investigate the subcellular distribution of protein kinase C gamma and delta in Purkinje cells of the rat cerebellum. Postembedding incubation with an antibody raised to a peptide sequence near the C-terminus of protein kinase C gamma resulted in strong labelling al...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00305-9
更新日期：1998-02-01 00:00:00
abstract：:Hypocretins (orexins) are hypothalamic neuropeptides that play a crucial role in regulating sleep/wake states and autonomic functions including parasympathetic cardiac activity. We have recently demonstrated stimulation of the lateral paragigantocellular nucleus (LPGi), the nucleus which is thought to play a role in r...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.11.067
更新日期：2011-02-23 00:00:00
abstract：:Thiamine deficiency during embryonic or early postnatal development causes deficits in cerebellum-dependent activities including motor control and procedural memory. Here, we give a detailed description of the changes to A-type current in cultured cerebellar granule neurons exposed to thiamine deficiency in vitro. A-t...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.06.053
更新日期：2012-09-27 00:00:00
abstract：:Known to exert an important modulatory influence on the cerebral cortex, the cholinergic neurons of the basal forebrain are modulated in turn by neurotransmitters which may include acetylcholine released from processes of brainstem or forebrain neurons. In the present study, we examined the effect of carbachol, a non-...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00167-x
更新日期：1997-11-01 00:00:00
abstract：:The purpose of the following experiments was to describe some of the neurochemical changes that occur in the basal ganglia of rats exposed chronically to a classical neuroleptic, fluphenazine, and to relate these changes to extrapyramidal motor dysfunction. For these studies a combination of behavioural, receptor auto...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90568-1
更新日期：1994-12-01 00:00:00
abstract：:The central mechanism of fever induction is triggered by an action of prostaglandin E(2) (PGE(2)) on neurons in the preoptic area (POA) through the EP3 subtype of prostaglandin E receptor. EP3 receptor (EP3R)-expressing POA neurons project directly to the dorsomedial hypothalamus (DMH) and to the rostral raphe pallidu...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.03.041
更新日期：2009-06-30 00:00:00
abstract：:Microdialysis was combined with a highly sensitive sequential multiple antigen radioimmunoassay to simultaneously measure extracellular cholecystokinin and neurotensin fragments from discrete regions of the rat brain in vivo. The assay was conducted in 96-well plates and provided a limit of detection for both peptides...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90105-w
更新日期：1991-01-01 00:00:00
abstract：:Several studies suggest that hypothalamic cocaine- and amphetamine-regulated transcript (CART) may interact with the hypothalamic-pituitary-adrenal (HPA) axis in the control of neuroendocrine function and may also participate in cardiovascular regulation. Therefore, this study aimed to evaluate, in experimental models...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.04.047
更新日期：2011-07-14 00:00:00
abstract：:The roles of acetylcholine and dynorphin (1-13) in the presynaptic control of the release of [3H]dopamine continuously synthesized from [3H]tyrosine were examined in a prominent striosomal enriched area and in an adjacent matrix enriched area of the cat caudate nucleus. This was achieved using microsuperfusion devices...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90340-t
更新日期：1991-01-01 00:00:00
abstract：:Cholinergic and glutamatergic cells in the pedunculopontine tegmental nucleus are a principal source of excitatory input to midbrain dopamine neurons projecting to the striatum. Disruption of these brainstem inputs has been shown to respectively enhance and reduce psychostimulant and opiate self-administration in rats...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(01)00595-4
更新日期：2002-01-01 00:00:00
abstract：:Alternative motor responses can be prepared in parallel. Here, we used electroencephalography (EEG) to test whether the parallel preparation of alternative response options is modulated by their relative value. Participants performed a choice response task with three potential actions: isometric contraction of the lef...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2018.01.055
更新日期：2018-03-15 00:00:00
abstract：:Recent findings indicate that neurosteroids could act as important keys during the brain development. Fluctuations in neonatal allopregnanolone (AlloP) could result in altered pharmacological properties of the GABA(A) receptor system in adulthood. Recent studies demonstrated that neurosteroids play a critical role in ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2008.04.062
更新日期：2008-07-17 00:00:00
abstract：:The possible neuroprotective effect of physical exercise was investigated in rats after middle cerebral artery occlusion (MCAO), a focal stroke model. It was found that physical exercise in the form of a 12-week treadmill running programme reduced the volume of infarction caused by MCAO. At the molecular level, revers...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00989-2
更新日期：2003-01-01 00:00:00
abstract：:Motor commands issued by the CNS are based upon memories of past experiences with similar objects, the current state of the hand and arm postures, and sensory input. Thus widespread somatosensory information is available to form precise representations of hand shape on which to base motor commands to match a desired p...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.02.047
更新日期：2015-05-07 00:00:00
abstract：:The lack of environmental olfactory stimulation produced by sensory deprivation causes significant changes in the deprived olfactory bulb. Olfactory transmission in the main olfactory bulb (MOB) is strongly modulated by centrifugal systems. The present report examines the effects of unilateral deprivation on the norad...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.05.046
更新日期：2006-09-15 00:00:00
abstract：:Up-regulation of proBDNF in ischemic brain and the detrimental role of proBDNF on cellular survival has already been established. We propose that the up-regulated proBDNF may trigger the harmful events and evoke a secondary ischemic damage after ischemia. This study aimed to establish the neuroprotective effects of an...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.08.008
更新日期：2020-10-15 00:00:00
abstract：:Rats received bilateral lesions of the nucleus basalis magnocellularis by infusion of ibotenic acid. Fourteen days later, osmotic minipumps releasing human recombinant nerve growth factor (0.3 micrograms/day) were implanted subcutaneously. Starting one month after the lesion, spatial learning of the animals was tested...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90342-y
更新日期：1992-01-01 00:00:00
abstract：:Net-like structures, visualized with the Golgi technique and several histochemical and immunocytochemical methods, have been described to ensheath somata, parts of dendrites and axon initial segments of various types of neurons. The origin and function of these perineuronal nets have been controversially discussed. Re...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90044-2
更新日期：1994-01-01 00:00:00
abstract：:The ventromedial spinal cord of mammals contains a neural network known as the locomotor central pattern generator (CPG) which underlies the basic generation and coordination of muscle activity during walking. To understand how this neural network operates, it is necessary to identify, characterize, and map connectivi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2013.07.070
更新日期：2013-10-10 00:00:00
abstract：:Thalamocortical neurons display a wide spectrum of activity patterns that are the expressions of the non-linear interactions between the various voltage-gated ion channels. Here, we show how bistable behaviour can emerge in these neurons, and how it is brought about by the steady-state residual ("window") component of...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00118-3
更新日期：1998-11-01 00:00:00
abstract：:A transient aberrant projection from the retina to the contralateral inferior colliculus was demonstrated in pigmented rats in both whole-brains and sections following intra-ocular injection of horseradish peroxidase. The projection was prominent on the day of birth but reached its maximum density and extent after inj...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(90)90087-k
更新日期：1990-01-01 00:00:00
abstract：:The sphenopalatine ganglion (SPG) is a gathering of the cell bodies of parasympathetic fibers that dominate the nasal gland, lacrimal gland and cerebral blood vessels. The SPG controls nasal secretions, tears, and the dilation of cerebral blood vessels. However, it is unclear how serotonin regulates SPG functions. In ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.05.028
更新日期：2019-07-15 00:00:00
abstract：:Prostaglandin E2 (PGE2) produced in the medial preoptic region (MPO) in response to immune signals is generally accepted to play a major role in triggering the illness response, a complex of physiological and behavioral changes induced by infection or injury. Hyperalgesia is now thought to be an important component of...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.06.050
更新日期：2004-01-01 00:00:00
abstract：:Sleep is strongly implicated in learning, especially in the reprocessing of recently acquired memory. Children with intellectual disability (ID) tend to have sleep-wake disturbances, which may contribute to the pathophysiology of the disease. Given that sleep is partly controlled by the circadian clock, we decided to ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.08.066
更新日期：2015-11-12 00:00:00
abstract：:The tachykinin peptide substance P (SP) is expressed by many interneurons and some projection neurons in the superficial dorsal horn of the spinal cord. We have recently shown that SP-expressing excitatory interneurons in lamina II correspond largely to a morphological class known as radial cells. However, little is k...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.06.038
更新日期：2020-12-01 00:00:00
abstract：:The effects of regular exercise versus a single bout of exercise on cognition, anxiety, and mood were systematically examined in healthy, sedentary young adults who were genotyped to determine brain-derived neurotrophic factor (BDNF) allelic status (i.e., Val-Val or Val66Met polymorphism). Participants were evaluated ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.04.056
更新日期：2012-07-26 00:00:00
abstract：:The ontogeny of substance P-containing neuron system in the forebrain and upper brain stem of the rat was investigated by means of the indirect immunofluorescence technique. Substance P-positive structures first appeared in the primordium of the epithalamus and the area which surrounded the commissura posterior of the...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(82)90165-8
更新日期：1982-01-01 00:00:00
abstract：:GABA(B) receptors are G-protein-coupled receptors mediating the slow onset and prolonged synaptic actions of GABA in the CNS. The recent cloning of two genes, GABA(B1) and GABA(B2), has revealed a novel requirement for GABA(B) receptor signalling. Studies have demonstrated that the two receptor subunits associate as a...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(01)00296-2
更新日期：2001-01-01 00:00:00
abstract：:There are declines in the protein expression of the NR2B (mouse epsilon2) and NR1 (mouse zeta1) subunits of the N-methyl-D-aspartate (NMDA) receptor in the cerebral cortex and hippocampus during aging in C57BL/6 mice. This study was designed to determine if there is a greater effect of aging on subunit expression and ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.05.018
更新日期：2009-09-15 00:00:00
