Increased regulatory B cells are involved in immune evasion in patients with gastric cancer.

Abstract:

:Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of tumoral immune evasion. However, the role of regulatory B cells (Bregs) in this remains unclear. Here, we identified a role for Bregs in immune evasion in gastric cancer (GC) patients. The frequency of peripheral Bregs was significantly higher in GC patients than in healthy controls (P = 0.0023). Moreover, the frequency of CD19+CD24hiCD27+ B cells in GC tissue was significantly higher than in peripheral blood and healthy gastric tissue. Carboxyfluorescein succinimidyl ester labeling revealed that CD19+CD24hiCD27+ B cells could suppress the proliferation of autologous CD4+ T cells. Moreover, CD19+CD24hiCD27+ B cells inhibited the production of interferon-gamma by CD4+ T cells. Double staining immunohistochemistry of interleukin-10 and CD19 revealed 5-year overall survival rates of 65.4% and 13.3% in BregLow and BregHigh groups, respectively (P < 0.0001). Multivariate analysis indicated that the frequency of Bregs was an independent prognostic indicator in GC patients. Taken together, our results show the existence of Bregs in GC tissue, and indicate that they are significantly correlated with the prognosis of GC patients.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Murakami Y,Saito H,Shimizu S,Kono Y,Shishido Y,Miyatani K,Matsunaga T,Fukumoto Y,Ashida K,Sakabe T,Nakayama Y,Fujiwara Y

doi

10.1038/s41598-019-49581-4

subject

Has Abstract

pub_date

2019-09-11 00:00:00

pages

13083

issue

1

issn

2045-2322

pii

10.1038/s41598-019-49581-4

journal_volume

9

pub_type

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