Abstract:
:Bacterial proteins exported to the cell surface play key cellular functions. However, despite the interest to study the localisation of surface proteins such as adhesins, transporters or hydrolases, monitoring their dynamics in live imaging remains challenging, due to the limited availability of fluorescent probes remaining functional after secretion. In this work, we used the Escherichia coli intimin and the Listeria monocytogenes InlB invasin as surface exposed scaffolds fused with the recently developed chemogenetic fluorescent reporter protein FAST. Using both membrane permeant (HBR-3,5DM) and non-permeant (HBRAA-3E) fluorogens that fluoresce upon binding to FAST, we demonstrated that fully functional FAST can be exposed at the cell surface and used to specifically tag the external side of the bacterial envelop in both diderm and monoderm bacteria. Our work opens new avenues to study the organization and dynamics of the bacterial cell surface proteins.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Chekli Y,Peron-Cane C,Dell'Arciprete D,Allemand JF,Li C,Ghigo JM,Gautier A,Lebreton A,Desprat N,Beloin Cdoi
10.1038/s41598-020-72498-2subject
Has Abstractpub_date
2020-09-25 00:00:00pages
15791issue
1issn
2045-2322pii
10.1038/s41598-020-72498-2journal_volume
10pub_type
杂志文章abstract::In Eastern Boundary Current systems, wind-driven upwelling drives nutrient-rich water to the ocean surface, making these regions among the most productive on Earth. Regulation of productivity by changing wind and/or nutrient conditions can dramatically impact ecosystem functioning, though the mechanisms are not well u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27612
更新日期:2016-06-09 00:00:00
abstract::Nanotips made of metal and semiconductor have been widely utilized in versatile applications to strengthen the electric field through lightning rod effect and localized surface plasmon resonance (LSPR) effect. Here, we present the utilization of ferroelectric nanotips to assist photoreduction of silver nanostructures ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47523-8
更新日期:2019-07-29 00:00:00
abstract::Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts. To understand how cells choose Pols when the replication fork encounte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13080-1
更新日期:2017-10-16 00:00:00
abstract::Cigarette smoking is a risk factor for stroke and is linked to stroke severity. Previous studies have shown that cigarette smoke extract (CSE) triggers endothelial dysfunction in vitro by initiating oxidative stress and/or an inflammatory response. In addition, cerebral endothelial dysfunction (particularly at the lev...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51728-2
更新日期:2019-10-30 00:00:00
abstract::Geometric mean concentrations of fecal indicator bacteria E. coli and enterococci are commonly used to evaluate the microbial quality of irrigation, recreation, and other types of waters, as well in watershed-scale microbial water quality modeling. It is not known how the uncertainty of those geometric mean concentrat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58603-5
更新日期:2020-02-03 00:00:00
abstract::GlgE, an enzyme of the pathway that converts trehalose to α-glucans, is essential for Mycobacterium tuberculosis. Inhibition of GlgE, which transfers maltose from a maltose-1-phosphate donor to α-glucan/maltooligosaccharide chain acceptor, leads to a toxic accumulation of maltose-1-phosphate that culminates in cellula...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17144
更新日期:2015-11-30 00:00:00
abstract::Land use, habitat, and forage quality have emerged as critical factors influencing the health, productivity, and survival of honey bee colonies. However, characterization of the mechanistic relationship between differential land-use conditions and ultimate outcomes for honey bee colonies has been elusive. We assessed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52485-y
更新日期:2019-11-07 00:00:00
abstract::Waste biomass from forestry and wood processing industries is a source to obtain fine chemicals, and its processing is a good example of circular economy, but it generates secondary environmental impacts. The main objective of this study was to analyse the environmental performances of laboratory scale processes for p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70587-w
更新日期:2020-08-12 00:00:00
abstract::Despite advances in therapy, glioblastoma remains an incurable disease with a dismal prognosis. Recent studies have implicated cancer stem cells within glioblastoma (glioma stem cells, GSCs) as mediators of therapeutic resistance and tumor progression. In this study, we investigated the role of the transforming growth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51270-1
更新日期:2019-10-10 00:00:00
abstract::The inhibitory behaviour of non-ionic sugar based N,N'-didodecyl-N,N'-digluconamideethylenediamine gemini surfactant, designated as Glu(12)-2-Glu(12) on mild steel (MS) corrosion in 3.5% NaCl at 30-60 °C was explored using weight loss, PDP, EIS and SEM/EDAX/AFM techniques. The compound inhibited the corrosion of mild ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21175-6
更新日期:2018-02-27 00:00:00
abstract::In Australia, significant shifts in species distribution have occurred with the loss of megafauna, changes in indigenous Australian fire regime and land-use changes with European settlement. The emu, one of the last megafaunal species in Australia, has likely undergone substantial distribution changes, particularly ne...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79551-0
更新日期:2021-01-13 00:00:00
abstract::Parasites and pathogens of the honey bee (Apis mellifera) are key factors underlying colony losses, which are threatening the beekeeping industry and agriculture as a whole. To control the spread and development of pathogen infections within the colony, honey bees use plant resins with antibiotic activity, but little ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06488-2
更新日期:2017-07-24 00:00:00
abstract::Different grazing strategies impact grassland plant production and may also regulate the soil carbon formation. For a site in semiarid temperate steppe, we studied the effect of combinations of rest, high and moderate grazing pressure over three stages of the growing season, on the process involved in soil carbon sequ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10892
更新日期:2015-07-03 00:00:00
abstract::Traffic-related air pollution is known to be associated with skin aging manifestations. We previously found that the use of fossil fuels was associated with skin aging, but no direct link between indoor air pollutants and skin aging manifestations has ever been shown. Here we directly measured the indoor PM2.5 exposur...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-15295-8
更新日期:2017-11-10 00:00:00
abstract::Success of immunotherapeutic approaches using genetically engineered antibodies and T cells modified with chimeric antigen receptors (CARs) depends, among other things, on the selection of antigen binding domains with desirable expression and binding characteristics. We developed a luciferase-based assay, termed Malib...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59099-9
更新日期:2020-02-11 00:00:00
abstract::During maternal recognition of pregnancy (MRP), a conceptus-derived signal leads to the persistence of the corpus luteum and the maintenance of gestation. In the horse, the nature of this signal remains to be elucidated. Several studies have focused on the changes in gene expression during MRP, but little information ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23537-6
更新日期:2018-03-27 00:00:00
abstract::In our experiment, it was observed that the emission of direct-gap band in germanium with Ge-GeSn layers on one-dimensional (1D) structure. The results of experiment and calculation demonstrate that the uniaxial tensile strain in the (111) and (110) direction can efficiently transform Ge to a direct bandgap material w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24802
更新日期:2016-04-21 00:00:00
abstract::Ebola virus (EBOV), isolate Makona, was the causative agent of the West African epidemic devastating predominantly Guinea, Liberia and Sierra Leone from 2013-2016. While several experimental vaccine and treatment approaches have been accelerated through human clinical trials, there is still no approved countermeasure ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-19041-y
更新日期:2018-01-16 00:00:00
abstract::We report on an angle resolved photoemission (ARPES) study of bulk electron-doped perovskite iridate, (Sr(1-x)La(x))3Ir2O7. Fermi surface pockets are observed with a total electron count in keeping with that expected from La substitution. Depending on the energy and polarization of the incident photons, these pockets ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08533
更新日期:2015-02-23 00:00:00
abstract::The ZIC proteins are a family of transcription regulators with a well-defined zinc finger DNA-binding domain and there is evidence that they elicit functional DNA binding at a ZIC DNA binding site. Little is known, however, regarding domains within ZIC proteins that confer trans-activation or -repression. To address t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69917-9
更新日期:2020-08-04 00:00:00
abstract::In this paper, a new type of porous ceramics was prepared using the raw sepiolite mineral. The porous ceramics was shaped by the dry pressing method and sintered in the range of 700 ~ 1200 °C. The temperature-microstructure evolution and the properties of porous sepiolite ceramics were investigated by thermo gravimetr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43918-9
更新日期:2019-05-14 00:00:00
abstract::A detailed characterisation of the molecular determinants of membrane binding by α-synuclein (αS), a 140-residue protein whose aggregation is associated with Parkinson's disease, is of fundamental significance to clarify the manner in which the balance between functional and dysfunctional processes are regulated for t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27125
更新日期:2016-06-08 00:00:00
abstract::T2-weighted cardiovascular magnetic resonance (CMR) using a 3-slice approach has been shown to accurately quantify the edema-based area-at-risk (AAR) in ST-segment elevation myocardial infarction (STEMI). We aimed to compare the performance of a 3-slice approach to full left ventricular (LV) coverage for the AAR by T1...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05127-0
更新日期:2017-07-07 00:00:00
abstract::Bacteria swim in sequences of straight runs that are interrupted by turning events. They drive their swimming locomotion with the help of rotating helical flagella. Depending on the number of flagella and their arrangement across the cell body, different run-and-turn patterns can be observed. Here, we present fluoresc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16428-9
更新日期:2017-12-01 00:00:00
abstract::Staphylococcus epidermidis is the predominant cause of recalcitrant biofilm-associated infections, which are often highly resistant to antibiotics. Thus, the use of physico-chemical agents for disinfection offers a more effective approach to the control of S. epidermidis biofilm infections. However, the underlying tol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41797-8
更新日期:2019-03-29 00:00:00
abstract::Dyschloremia is common in critically ill patients. However, little is known about the effects of dyschloremia on renal function in patients with acute kidney injury (AKI) requiring continuous renal replacement therapy (CRRT). A total of 483 patients who received CRRT for AKI were selected and divided into three groups...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76798-5
更新日期:2020-11-12 00:00:00
abstract::A feasibility study is presented on the performance parameters of a novel on-chip antenna based on metasurface technology at terahertz band. The proposed metasurface on-chip antenna is constructed on an electrically thin high-permittivity gallium arsenide (GaAs) substrate layer. Metasurface is implemented by engraving...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68105-z
更新日期:2020-07-03 00:00:00
abstract::Real-valued functions of complex arguments violate the Cauchy-Riemann conditions and, consequently, do not have Taylor series expansion. Therefore, optimization methods based on derivatives cannot be directly applied to this class of functions. This is circumvented by mapping the problem to the field of the real numbe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52289-0
更新日期:2019-11-06 00:00:00
abstract::We introduce "sense, track and separate" approach for the removal of Hg(2+) ion from aqueous media using highly ordered and magnetic mesoporous ferrosilicate nanocages functionalised with rhodamine fluorophore derivative. These functionalised materials offer both fluorescent and magnetic properties in a single system ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21820
更新日期:2016-02-25 00:00:00
abstract::Recently, a large number of long noncoding RNAs (lncRNAs) have emerged as important regulators of many biological processes in animals and plants. However, how lncRNAs function during plant DNA virus infection is largely unknown. We performed strand-specific paired-end RNA sequencing of tomato samples infected with To...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16946
更新日期:2015-12-18 00:00:00