Introducing Vibrio natriegens as a Microbial Model Organism for Microgravity Research.

Abstract:

:Microbial contamination of human-tended spacecraft is unavoidable, making the study of microbial growth under space conditions essential for the preservation of astronauts' health and equipment integrity. Previous studies suggested that spaceflight conditions, such as microgravity, cause a range of physiological microbial alterations including increased growth yields and decreased antibiotic susceptibility. Because of its fast generation time, Vibrio natriegens could be used as a model organism for a variety of studies where generation time is a critical factor. In this study, V. natriegens was used as a tool to study growth characteristics by determining the viable cell number and antibiotic susceptibility under simulated microgravity using a 2-D clinostat (60 rpm) to establish a test system that resolves changes in microbial growth on a solid surface (agar) under microgravity. The data show that V. natriegens biomass increases significantly after 24 h at 37°C under simulated microgravity. The final cell population after cultivation under simulated microgravity was 60-fold greater than when cultivated under normal terrestrial gravity (1 × g). No change in susceptibility to the antibiotic rifampicin after cultivation under simulated microgravity or normal gravity was detected. These data show that V. natriegens is a new and innovative model organism for microbial microgravity research.

journal_name

Astrobiology

journal_title

Astrobiology

authors

Garschagen LS,Mancinelli RL,Moeller R

doi

10.1089/ast.2018.2010

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

1211-1220

issue

10

eissn

1531-1074

issn

1557-8070

journal_volume

19

pub_type

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