Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:Liuwei Dihuang (LWDH) is a classic prescription that has been used as a traditional medicinal formula for more than 1000 years in China. In clinical, LWDF is used for treating functional decline associated with senile disease and menopausal syndrome. Studies have demonstrated that LWDH could significantly improve estrogen level and ER expression, and suspend the process of atherosclerosis. However, the under mechanism of how LWDH suppressing VSMCs phenotypic conversion and proliferation through ER is still unknown. AIM OF THE STUDY:This study was to reveal the under mechanism of how LWDH inhibits the phenotypic conversion of VSMCs. MATERIALS AND METHODS:24 ApoE-/- mice were divided into 4 groups: sham group, model group, E2 group, and LWDH group, and 6 C57BN/L6 mice were used as control group. The primary VSMCs were divided into control group, model group, E2 group, LWDH group, LWDH + MPP group, and LWDH + PHTPP group with or without control siRNA, ERα siRNA, ERβ siRNA, and myocardin siRNA. Oil red staining was used to evaluate the lipid deposition in the cardiac aorta. Serum chemistry analysis to test serum TG, TC, LDL, and HDL. Immunofluorescence staining was used to test α-SMA, osteopontin and F-actin. Immunohistochemical staining was performed to check out the myocardin in the cardiac aorta. The mRNA levels of α-SMA, osteopontin, ERα, ERβ, SRC3 and myocardin were detected by Real Time-PCR, and the protein expression levels of them were detected by Western blotting. Co-immunoprecipitation was proceed to test the interaction between ERα and SRC3 and SRC3 and myocardin. Flow cytometry was used to check out the cell cycle. Wound healing assay and Transwell were managed to evaluate the migration capacity of VSMCs. RESULTS:In vivo administration of LWDH suppressed AS symptoms, decreases phenotypic marker of vascular endothelial cell, and increases phenotypic marker of VSMC in ovariectomized ApoE-/- female mice. Moreover, LWDH significantly increased the mRNA and protein expression levels of ERα, ERβ, SRC3 and myocardin in the cardiac aorta of ovariectomized ApoE-/- female mice. In vitro, LWDH altered cell cycle and reduced the elevated cyclinD protein expression migration capacity and in the model VSMCs. In addition, LWDH inhibited phenotypic conversion and promoted the expression of ER, SRC3, and myocardin of the primary VSMC phenotypic conversion model. Inhibition of ERα almost completely eliminated the impacts of LWDH on α- SMA and osteopontin. Furthermore, LWDH promoted the interaction between ERα and SRC3 and up-regulated the co-activation of SRC3 and myocardin. CONCLUSIONS:LWDH could inhibit the phenotypic conversion of VSMCs in vitro and in vivo by increasing the activity of myocardin through up-regulating the expression of ERα and promoting the interaction between ERα and SRC3. Our research reveals the under mechanism of how LWDH inhibits the phenotypic conversion of VSMCs.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Meng Q,Yu X,Chen Q,Wu X,Kong X,Wang S,Cai D,Cheng P,Li Y,Bian Hdoi
10.1016/j.jep.2019.112207subject
Has Abstractpub_date
2020-01-10 00:00:00pages
112207eissn
0378-8741issn
1872-7573pii
S0378-8741(19)31415-1journal_volume
246pub_type
杂志文章abstract::Lyophilised hydroalcoholic and aqueous extracts of the aerial parts of Passiflora incarnata L. (Passifloraceae) (Passion-flower), as well as chemical constituents of the plant, indole alkaloids (harman, harmin, harmalin, harmol, and harmalol) maltol and flavonoids (orientin, isoorientin, vitexin and isovitexin) were a...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(97)00042-1
更新日期:1997-06-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The study was done to establish medicinal plants used in the treatment of various diseases by the people in the Northern sector of Kibale National Park in western Uganda. It was also aimed at establishing the plant parts used and the mode of preparation of remedies. These plants create a ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.04.044
更新日期:2011-06-14 00:00:00
abstract::Melissa officinalis L. (Labiatae), Morus alba L. (Moraceae), and Artemisia capillaris Thunb. (Compositae) are suggested to be involved in the regulation of hyperlipidemia. We hypothesized that Ob-X, a mixture of three herbs, Morus alba, Melissa officinalis and Artemisia capillaris [corrected] improves lipid metabolism...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2007.09.029
更新日期:2008-01-17 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Malaria remains a major global health threat, with the heaviest burden of disease in sub-Saharan Africa. Effective treatment is not available in many affected areas, and the Plasmodium falciparum parasite is becoming resistant to existing drugs. Alternative therapies are necessary to over...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.09.010
更新日期:2015-12-04 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:[corrected] In Taiwanese folk medicine, Davallia formosana is used to treat bone diseases, including osteoporosis. AIM:This study evaluated the anti-osteoporotic effect of ethanolic extract derived from Davallia formosana (DFE). MATERIALS AND METHODS:In this in vitro study, we investiga...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.11.050
更新日期:2012-01-31 00:00:00
abstract:BACKGROUND:The pharmacology, toxicology and pharmacokinetics of bioactive preparations derived from natural sources has become a flourishing field of research. However, researching complex extracts and natural products faces numerous challenges. More broadly in recent years the critique of pharmacological research, and...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2019.112230
更新日期:2020-01-10 00:00:00
abstract::Total glucosides of paeony (TGP) is the major active constituent of Paeonia lactiflora Pall. The present study was carried out to investigate the effects of TGP on adjuvant arthritis (AA) of rat and its possible mechanisms. AA was induced by metatarsal footpad injection with complete Freund's adjuvant in male Sprague-...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.08.019
更新日期:2007-02-12 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:As an important medicinal material constituting a variety of traditional Chinese medicine prescriptions, Nepeta angustifolia C. Y. Wu was used as a folk medicine to treat various vascular-related diseases including apoplexia, and cerebral haemorrhage in Tibet, China. Our previous studies ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112771
更新日期:2020-06-12 00:00:00
abstract:AIM OF THE STUDY:We sought to determine the efficacy as antioxidant and safety profile of the polyherbal formulation in geriatric patients of eastern India. MATERIALS AND METHODS:The study was double-blind, randomized including placebo controlled and was approved by the ethical committee of SSKM hospital. Geriatric pa...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jep.2010.12.044
更新日期:2011-03-24 00:00:00
abstract::Traditional medicine (TM) in Bulamogi (Uganda) is holistic, providing treatments for physical illnesses as well as psycho-spiritual ones. People use it to prevent and eliminate the effects of witchcraft, to appease spirits and to cure chronic illnesses. The traditional medicine practitioners (TMPs) are numerous and ha...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(02)00378-1
更新日期:2003-03-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Cistanche tubulosa (Schenk) R. Wight has been used frequently in traditional folk medicine for treatment of male sexual dysfunction (MSD). Phenylethanol glycosides, the main components of C. tubulosa, possess a variety of pharmacological activities due to their multiple properties. Howeve...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112500
更新日期:2020-04-06 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Rosmarinic acid (RA), a caffeic acid-related compound found in high concentrations in Prunella vulgaris (self-heal), and ursolic acid (UA), a pentacyclic triterpene acid concentrated in Salvia officinalis (sage), have been traditionally used to treat inflammation in the mouth, and may als...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.07.037
更新日期:2011-10-11 00:00:00
abstract:AIM OF THE STUDY:Scutellaria barbata is a traditional Chinese medicine for cancer treatments. Pheophorbide-a (Pa), one of the active components isolated from this herbal medicine has been proposed to be a potential natural photosensitizer for photodynamic therapy. The anti-tumor effect of pheophorbide-a based photodyna...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.06.007
更新日期:2010-08-19 00:00:00
abstract::Aqueous, ethanolic and hexane fractions of Momordica cymbalaria fruits were prepared and given individually at different doses to different batches of rats (both normal and alloxan diabetic rats) after an overnight fast. The blood glucose levels were measured at 0, 1, 3, 5 and 7 h after the treatment. The aqueous extr...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(01)00324-5
更新日期:2001-11-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Meta-analysis (MA) on Chinese herbal medicine (CHM) trials is increasingly published and indexed in major international databases but their trustworthiness and clinical applicability is uncertain. We aimed to assess the characteristics and methodological quality of MA on CHM. MATERIALS A...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2014.12.028
更新日期:2015-03-13 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Pinellia pedatisecta Schott (PPS)is a traditional Chinese medicine functioning as reducing swelling and drying dampness. Pinellia pedatisecta Schott extract (PE) has been confirmed to suppress cervical tumor growth and modulate the antitumor CD4+T helper immunity towards Th1. AIMS:To exp...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.111980
更新日期:2019-09-15 00:00:00
abstract:ETHNO-PHARMACOLOGICAL RELEVANCE:Picrosides I, II and apocynin are the main active principles present in the roots and rhizomes of Picrorhiza kurroa Royle ex. Benth (Kutki). Ethno-medicinally, the plant is used for the treatment of liver, upper respiratory tract disorders and dyspepsia, since long in Ayurveda. AIM OF T...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.07.072
更新日期:2017-02-02 00:00:00
abstract:AIM OF STUDY:Diabetes mellitus is frequently combined with vascular diseases, which are associated with the expression of vascular endothelial growth factor (VEGF). An approach that can reverse the induction of VEGF by hyperglycemia may potentially benefit the outcome of diabetic patients. Therefore, in the present stu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.07.018
更新日期:2011-09-02 00:00:00
abstract:AIM OF THE STUDY:Artemisia vestita is a common traditional Tibetan medicinal plant which has been used widely in China for treating various inflammatory diseases. Since little is known about its active components, the purpose of this study was to isolate and identify the immunosuppressive compounds from Artemisia vesti...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.07.029
更新日期:2008-10-30 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Flourensia fiebrigii is a plant used in traditional medicine in the Argentine Calchaquí Valley as purgative, expectorant, anti-rheumatic and anti-inflammatory. AIM OF THE STUDY:The aim of this study was to analyze the macroscopic and microscopic characteristics of F. fiebrigii leaf and s...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113296
更新日期:2021-01-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:There is growing inclination towards developing bioactive molecule-based strategies for the management of allergic airway inflammation associated respiratory diseases. Vitex negundo Linn., also known as Nirgundi, is one such medicinal plant enriched with phytochemicals and used for inflam...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2021.113894
更新日期:2021-01-28 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ganoderma lucidum Polysaccharide (GLP),traditional Chinese medicine (TCM) active ingredient, has a long history and has good curative effects on radiation injury. However, the mechanism of GLP treating radiation injury has not been clearly elucidated. THE AIM OF THE STUDY:This study was ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112814
更新日期:2020-08-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Many studies have emphasized that flavonoids, found in various fruits, vegetables, and seeds, as well as tea and red wine, have potential health-promoting and disease-preventing effects. Rhamnetin is a flavonoid that exhibits antioxidant capabilities. However, little is known about its ef...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.02.019
更新日期:2014-05-14 00:00:00
abstract::Chenopodium ambrosioides L. and Chenopodium multifidum L. (Chenopodiaceae), common name: Paico, are medicinal plants. They are aromatic shrubs growing in South America. For centuries, they have been used due to its medicinal properties. However, there are few reports in literature about the genotoxic effects of these ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.08.043
更新日期:2006-01-16 00:00:00
abstract::Sixty-seven extracts of 30 medicinal plants traditionally used in New Caledonia or Vanuatu by healers to treat inflammation, fever and in cicatrizing remedies were evaluated in vitro for their antiprotozoal activity against Leishmania donovani, Leishmania amazonensis and Trypanosoma cruzi. Among the selected plants, P...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2004.10.006
更新日期:2005-01-15 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Radix Salvia Miltiorrhiza (Danshen) and Radix Pueraria Lobate (Gegen) are officially listed in the Chinese Pharmacopoeia and have long been used together as a Compound Chinese Traditional Medicine (CCTM) for treatment of coronary heart diseases, which are often co-administered with aspiri...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113151
更新日期:2020-11-15 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The rhizomes of Kaempferia parviflora Wall. ex Baker have been traditionally used in Thailand to treat abscesses, gout, and peptic ulcers. AIM:Previously, we reported that the chloroform fraction of a Kaempferia parviflora extract had an inhibitory effect on rat paw-edema. In the present...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.01.013
更新日期:2011-07-14 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Artemisia biennis Willd. (Dermane in Persian) has been used as an antinociceptive remedy in Iranian folkloric medicine. OBJECTIVE:The aim of the present study was to evaluate the anti-nociceptive effects of Artemisia biennis Willd. aerial part essential oil (ABAEO) on male Swiss mice. M...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113604
更新日期:2021-03-25 00:00:00
abstract:AIM OF THE STUDY:Insamhodo-tang (IHT) has traditionally been used in Korea to treat a variety of diseases, including chronic cough, tuberculosis, and chronic bronchitis. However, the anti-allergic and anti-inflammatory effects of IHT and its molecular mechanisms have yet to be clearly elucidated. In this study, we atte...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.12.023
更新日期:2011-03-24 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Hyptis martiusii (Lamiaceae), popularly known as "cidreira-do-mato" or "cidreira-brava", grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to charact...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.07.005
更新日期:2011-09-01 00:00:00