Abstract：
:The increasing antibiotic resistance evolved by Helicobacter pylori has alarmingly reduced the eradication rates of first-line therapies. To overcome the current circulating resistome, we selected a novel potential therapeutic target in order to identify new candidate drugs for treating H. pylori infection. We screened 1120 FDA-approved drugs for molecules that bind to the essential response regulator HsrA and potentially inhibit its biological function. Seven natural flavonoids were identified as HsrA binders. All of these compounds noticeably inhibited the in vitro DNA binding activity of HsrA, but only four of them, apigenin, chrysin, kaempferol and hesperetin, exhibited high bactericidal activities against H. pylori. Chrysin showed the most potent bactericidal activity and the most synergistic effect in combination with clarithromycin or metronidazole. Flavonoid binding to HsrA occurs preferably at its C-terminal effector domain, interacting with amino acid residues specifically involved in forming the helix-turn-helix DNA binding motif. Our results validate the use of HsrA as a novel and effective therapeutic target in H. pylori infection and provide molecular evidence of a novel antibacterial mechanism of some natural flavonoids against H. pylori. The results further support the valuable potential of natural flavonoids as candidate drugs for novel antibacterial strategies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
González A,Salillas S,Velázquez-Campoy A,Espinosa Angarica V,Fillat MF,Sancho J,Lanas Ádoi
10.1038/s41598-019-47746-9subject
Has Abstractpub_date
2019-08-05 00:00:00pages
11294issue
1issn
2045-2322pii
10.1038/s41598-019-47746-9journal_volume
9pub_type
杂志文章abstract：:Development of conformal n-channel organic phototransistor (OPT) array is urgent for future applications of organic complementary circuits in portable and wearable electronics and optoelectronics. In this work, the ultrathin conformal OPT array based on air-stable n-type PTCDI-C13H27 was fabricated. The OPT array show...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35062-7
更新日期：2018-11-09 00:00:00
abstract：:Few-layer black phosphorus (BP) is the most promising material among the two-dimensional materials due to its layered structure and the excellent semiconductor properties. Currently, thin BP atomic layers are obtained mostly by mechanical exfoliation of bulk BP, which limits applications in thin-film based electronics...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23736
更新日期：2016-03-30 00:00:00
abstract：:Lipid droplet (LD), a multi-functional organelle, is often found to associate with other cellular membranous structures and vary in size in a given cell, which may be related to their functional diversity. Here we established a method to separate LD subpopulations from isolated CHO K2 LDs into three different size cat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29539
更新日期：2016-07-08 00:00:00
abstract：:Unpleasant emotional distraction can impair the retention of non-emotional information in working memory (WM). Research links the prefrontal cortex with the successful control of such biologically relevant distractors, although the temporal changes in this brain mechanism remain unexplored. We use magnetoencephalograp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10046
更新日期：2015-06-12 00:00:00
abstract：:Drug discovery efforts against the pathogen Mycobacterium tuberculosis (Mtb) have been advanced through phenotypic screens of extensive compound libraries. Such a screen revealed sulfolane 1 and indoline-5-sulfonamides 2 and 3 as potent inhibitors of mycobacterial growth. Optimization in the sulfolane series led to co...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09642-y
更新日期：2017-08-25 00:00:00
abstract：:During our research, we explored a novel way to represent subwavelength imaging and derived a transmission equation to explicate the FP (Fabry-Pérot) resonance phenomena. Subsequently, using analysis and observation, we performed deep-subwavelength imaging. Both numerically and experimentally, imaging with super-resol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67409-4
更新日期：2020-07-01 00:00:00
abstract：:As part of efforts to reduce pressure on the Amazon and other biomes, one approach considered by Brazilian authorities and scientists is more intensive use of the soils of the interior of the northeast of the country, which are generally sandy, with low contents of organic matter and low water holding capacity and are...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28794-z
更新日期：2018-07-16 00:00:00
abstract：:To elucidate the temporal changes in the radiocesium distribution in forests contaminated by the Fukushima Daiichi Nuclear Power Plant accident, we monitored the 137Cs concentration and inventory within forests from 2011 to 2015 across nine plots containing variable tree species and different contamination levels. The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08261-x
更新日期：2017-08-15 00:00:00
abstract：:Fluorescence visualization devices (FVs) are useful for detecting malignant lesions because of their simple and noninvasive application. However, their quantitative application has been challenging. This study aimed to quantitatively and statistically evaluate the change in fluorescence intensity (FI) during the progr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44287-z
更新日期：2019-05-24 00:00:00
abstract：:In the Brown et al. study 'Increased food supply mitigates ocean acidification effects on calcification but exacerbates effects on growth' they show disagreement with the tested hypothesis and data analysis methodology used in our 2016 study. We acknowledge careful criticism and a constructive dialogue are necessary t...
journal_title：Scientific reports
pub_type： 评论,杂志文章
doi：10.1038/s41598-018-27670-0
更新日期：2018-06-28 00:00:00
abstract：:Soil respiration (Rs) has been usually measured during daylight hours using manual chambers. This approach assumes that measurements made during a typical time interval (e.g., 9 to 11 am) represent the mean daily value; locally, this may not always be correct and could result in systematic bias of daily and annual Rs ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11849-y
更新日期：2017-09-20 00:00:00
abstract：:Tropical deforestation is responsible for around one tenth of total anthropogenic carbon emissions, and tropical protected areas (PAs) that reduce deforestation can therefore play an important role in mitigating climate change and protecting biodiversity and ecosystem services. While the effectiveness of PAs in reduci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14467-w
更新日期：2017-10-25 00:00:00
abstract：:It is challenging to create artificial catalysts that approach enzymes with regard to catalytic efficiency and selectivity. The enantioselective catalysis ranks the privileged characteristic of enzymatic transformations. Here, we report two pyridine-linked bis(β-cyclodextrin) (bisCD) copper(II) complexes that enantios...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22080
更新日期：2016-02-26 00:00:00
abstract：:Electronic structures of graphene oxide (GO) and hydro-thermally reduced graphene oxides (rGOs) processed at low temperatures (120-180°C) were studied using X-ray absorption near-edge structure (XANES), X-ray emission spectroscopy (XES) and resonant inelastic X-ray scattering (RIXS). C K-edge XANES spectra of rGOs rev...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04525
更新日期：2014-04-10 00:00:00
abstract：:Odorant binding proteins (OBPs) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. The diving beetle Cybister japonicus Sharp shows a remarkable sexual dimorphism. The foreleg tarsi of males are equipped with large suction cups, believ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31848
更新日期：2016-08-22 00:00:00
abstract：:Animals thriving in hot deserts rely on extraordinary adaptations and thermoregulatory capacities to cope with heat. Uncovering such adaptations, and how they may be favoured by selection, is essential for predicting climate change impacts. Recently, the arid-adapted zebra finch was discovered to program their offspri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75909-6
更新日期：2020-11-03 00:00:00
abstract：:Magnetic small-angle neutron scattering (SANS) is a powerful technique for investigating magnetic nanoparticle assemblies in nonmagnetic matrices. For such microstructures, the standard theory of magnetic SANS assumes uniformly magnetized nanoparticles (macrospin model). However, there exist many experimental and theo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13457-2
更新日期：2017-10-12 00:00:00
abstract：:Behavioral addiction (BA) is characterized by repeated, impulsive and compulsive seeking of specific behaviors, even with consequent negative outcomes. In drug addiction, alterations in biological mechanisms, such as monoamines and epigenetic processes, have been suggested, whereas whether such mechanisms are also alt...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68741-5
更新日期：2020-07-16 00:00:00
abstract：:Phase transition is a natural phenomenon happened around our daily life, represented by the process from ice to water. While melting and solidifying at a certain temperature, a high heat of fusion is accompanied, classified as the latent heat. Phase change material (PCM) has been widely applied to store and release la...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08884
更新日期：2015-03-09 00:00:00
abstract：:Modulation of initial burst and long term release from electrospun fibrous mats can be achieved by sandwiching the drug loaded mats between hydrophobic layers of fibrous polycaprolactone (PCL). Ibuprofen (IBU) loaded PCL fibrous mats (12% PCL-IBU) were sandwiched between fibrous polycaprolactone layers during the proc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74885-1
更新日期：2020-10-23 00:00:00
abstract：:Perilla frutescens (L.) is an important medicinal and edible plant in China with nutritional and medical uses. The extract from leaves of Perilla frutescens contains flavonoids and volatile oils, which are mainly used in traditional Chinese medicine. In this study, we analyzed the transcriptomic and metabolomic data o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73274-y
更新日期：2020-10-01 00:00:00
abstract：:Electrochemical capacitors (EC) have received tremendous interest due to their high potential to satisfy the urgent demand in many advanced applications. The development of new electrode materials is considered to be the most promising approach to enhance the EC performance substantially. Herein, we present a high-cap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06083
更新日期：2014-08-15 00:00:00
abstract：:Lack of standardization and unblinding threaten the research of mechanisms involved in expectancy effects on pain. We evaluated a computer-controlled virtual experimenter (VEx) to avoid these issues. Fifty-four subjects underwent a baseline-retest heat pain protocol. Between sessions, they received an expectancy manip...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77453-9
更新日期：2020-11-23 00:00:00
abstract：:Morphological phylogenies stand in a major conflict with molecular hypotheses regarding the phylogeny of Cryptodira, the most diverse and widely distributed clade of extant turtles. However, molecular hypotheses are often considered a better estimate of phylogeny given that it is more consistent with the stratigraphic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16299
更新日期：2015-11-10 00:00:00
abstract：:The interpenetrating morphology formed by the electron donor and acceptor materials is critical for the performance of polymer:fullerene bulk heterojunction (BHJ) photovoltaic (PV) cells. In this work we carried out a systematic investigation on a high PV efficiency (>6%) BHJ system consisting of a newly developed 5,6...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05211
更新日期：2014-06-09 00:00:00
abstract：:The capacity of healthy adult listeners to accommodate to altered spectral cues to the source locations of broadband sounds has now been well documented. In recent years we have demonstrated that the degree and speed of accommodation are improved by using an integrated sensory-motor training protocol under anechoic co...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11981-9
更新日期：2017-09-14 00:00:00
abstract：:Multisensory integration provides continuous and stable perception from separate sensory inputs. Here, we investigated the functional role of temporal binding between the visual and the tactile senses. To this end we used the paradigm of compression that induces shifts in time when probe stimuli are degraded, e.g., by...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25843
更新日期：2016-05-16 00:00:00
abstract：:Emerging concepts for on-chip biotechnologies aim to replace microfluidic flow by active, molecular-motor driven transport of cytoskeletal filaments, including applications in bio-simulation, biocomputation, diagnostics, and drug screening. Many of these applications require reliable detection, with minimal data acqui...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01092
更新日期：2013-01-01 00:00:00
abstract：:We propose a novel scheme to realize an optical waveguide induced by an active Raman gain (ARG) process in a four-level N-type atomic system. Because of the nature of the ARG, there are two distinct features related to the waveguide: i) It is not absorptive, on the contrary, weak gain is presented; ii) It can be impro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14456-z
更新日期：2017-10-27 00:00:00
abstract：:This study investigates the immune responses induced by metal-filled single-walled carbon nanotubes (SWCNT) under in vitro, ex vivo and in vivo settings. Either empty amino-functionalized CNTs [SWCNT-NH2 (1)] or samarium chloride-filled amino-functionalized CNTs with [SmCl3@SWCNT-mAb (3)] or without [SmCl3@SWCNT-NH2 (...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42605
更新日期：2017-02-15 00:00:00
