Male tarsi specific odorant-binding proteins in the diving beetle Cybister japonicus sharp.

Abstract:

:Odorant binding proteins (OBPs) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. The diving beetle Cybister japonicus Sharp shows a remarkable sexual dimorphism. The foreleg tarsi of males are equipped with large suction cups, believed to help holding the female during underwater courtship and mating. Here, we identified two OBPs highly and specifically expressed in male tarsi, suggesting important functions of these structures in chemical communication. The first protein, CjapOBP1, exhibits the 6 conserved cysteines motif of classic OBPs, while the second, CjapOBP2, contains only four cysteines and can be assigned to the sub-class of C-minus OBPs. Both proteins were expressed in a bacterial system and the purified recombinant proteins were used to for antibodies preparation. Western Blot analysis showed that CjapOBP1 is predominantly expressed in male tarsi and could be also detected in antennae and palpi of both sexes, while CjapOBP2, besides male tarsi, is also present in testis. Ligand-binding experiments showed a good binding affinity between CjapOBP1, CjapOBP2 and citral and coniferyl aldehyde, respectively. These results support a possible function of these two OBPs in the male foreleg tarsi of diving beetles in chemical communication.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Song LM,Jiang X,Wang XM,Li JD,Zhu F,Tu XB,Zhang ZH,Ban LP

doi

10.1038/srep31848

subject

Has Abstract

pub_date

2016-08-22 00:00:00

pages

31848

issn

2045-2322

pii

srep31848

journal_volume

6

pub_type

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