Abstract:
PURPOSE:This study aimed to explore the effects and mechanisms of long non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the osteogenesis of osteoblast cells in postmenopausal osteoporosis (PMOP). MATERIALS AND METHODS:Mice models of PMOP were established. ANCR expression and intracellular calcium ions were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and laser confocal microscopy, respectively. ANCR was silenced in osteoblast cells from PMOP mice by the transfection of siRNA-ANCR (si-ANCR). The proliferation and apoptosis of osteoblast cells was analyzed by MTT and flow cytometry, respectively. Alkaline phosphatase (ALP) activity and calcium nodules were examined by ALP and alizarin red staining assay, respectively. The expression of enhancer of zeste homolog 2 (EZH2), runt related transcription factor 2 (RUNX2), and OSTERIX was detected by qRT-PCR and Western blot. Furthermore, an osteogenesis model was constructed in mice, and osteoid formation was observed by hematoxylin-eosin (HE) staining. The interaction between lncRNA-ANCR and EZH2 was further identified by RNA pull-down assay. RESULTS:ANCR expression and intracellular calcium ions were increased in PMOP mice. Si-ANCR significantly increased the proliferation, ALP activity, calcium deposition of osteoblast cells and decreased apoptosis. ANCR and EZH2 were down-regulated by si-ANCR, while RUNX2 and OSTERIX were upregulated. Si-ANCR also promoted osteoid formation in mice treated with hydroxyapatite-tricalcium phosphate. In addition, ANCR specifically bound to EZH2. CONCLUSION:Silencing ANCR promotes the osteogenesis of PMOP osteoblast cells. The specific binding of ANCR with EZH2 suppressed RUNX2, thereby inhibiting osteogenesis.
journal_name
Yonsei Med Jjournal_title
Yonsei medical journalauthors
Cai N,Li C,Wang Fdoi
10.3349/ymj.2019.60.8.751subject
Has Abstractpub_date
2019-08-01 00:00:00pages
751-759issue
8eissn
0513-5796issn
1976-2437pii
60.751journal_volume
60pub_type
杂志文章abstract::Long QT syndrome is associated with lethal tachyarrhythmia that can lead to syncope, seizure, and sudden death. Congenital long QT syndrome is a genetic disorder, characterized by delayed cardiac repolarization and prolongation of the QT interval on the electrocardiogram (ECG). Type 2 congenital long QT is linked to m...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2011.52.6.1025
更新日期:2011-11-01 00:00:00
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journal_title:Yonsei medical journal
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doi:10.3349/ymj.2012.53.5.968
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.2.516
更新日期:2013-03-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1998.39.3.247
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doi:10.3349/ymj.2018.59.2.303
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2016.57.4.950
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journal_title:Yonsei medical journal
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doi:10.3349/ymj.2018.59.3.383
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1990.31.4.375
更新日期:1990-12-01 00:00:00
abstract::This study was undertaken to evaluate the clinical usefulness of operative laparoscopy in treatment of benign ovarian cysts. A retrospective study was carried on 468 operative laparoscopy cases performed from September 1995 to September 1998 at Yonsei University College of Medicine, Department of Obstetrics and Gyneco...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1999.40.6.608
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abstract::A giant esophageal liposarcoma showing rapid growth over 7 months is presented in 56-year-old man. It originated from the pharyngo-esophageal junction with a short stalk, and extended downward to the distal esophagus. A barium swallow study showed a large, sausage-like intraluminal mass in the dilated esophagus. CT an...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2003.44.4.715
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journal_title:Yonsei medical journal
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doi:10.3349/ymj.2018.59.3.457
更新日期:2018-05-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.4.582
更新日期:2009-08-31 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1983.24.2.141
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abstract:PURPOSE:The aim of this study was to find an optimal range of activated clotting time (ACT) during off-pump coronary artery bypass surgery (OPCAB) yielding ischemic protection without the risk of hemorrhagic complications in patients with recent exposure to dual antiplatelet therapy. MATERIALS AND METHODS:Three hundre...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.5.1119
更新日期:2013-09-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1993.34.1.35
更新日期:1993-03-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2012.53.5.1036
更新日期:2012-09-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1996.37.2.97
更新日期:1996-04-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2010.51.5.740
更新日期:2010-09-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2003.44.6.1083
更新日期:2003-12-30 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2008.49.5.714
更新日期:2008-10-31 00:00:00
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journal_title:Yonsei medical journal
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doi:10.3349/ymj.2007.48.5.879
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2017.58.1.9
更新日期:2017-01-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
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更新日期:2000-12-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2001.42.1.41
更新日期:2001-02-01 00:00:00
abstract:PURPOSE:Tenofovir disoproxil fumarate (TDF) is commonly prescribed as a fixed-dose, co-formulated antiretroviral drug for HIV-1 infection. The major concern of long-term TDF use is renal dysfunction. However, little is known about the long-term patterns of changes in renal function in HIV-infected Koreans receiving TDF...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2017.58.4.770
更新日期:2017-07-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2003.44.1.150
更新日期:2003-02-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
doi:10.3349/ymj.2009.50.3.309
更新日期:2009-06-30 00:00:00
abstract::The 5'- and 3'-side half of liver type glucose transporter (GLUT2) cDNA was amplified from total RNA or mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). The amplified 5'-side fragment of GLUT2 cDNA was inserted into pGEM4Z and named pGLGT1, and the 3'-side fragment of GLUT2 cDNA was inserted into the ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1993.34.2.117
更新日期:1993-06-01 00:00:00
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journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1994.35.3.314
更新日期:1994-09-01 00:00:00