Abstract:
:The 5'- and 3'-side half of liver type glucose transporter (GLUT2) cDNA was amplified from total RNA or mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). The amplified 5'-side fragment of GLUT2 cDNA was inserted into pGEM4Z and named pGLGT1, and the 3'-side fragment of GLUT2 cDNA was inserted into the HindIII site of pGLGT1 to construct pGLGT2 which contains an entire open reading frame of GLUT2 cDNA. The GLUT2 cDNA in pGLGT2 was transferred to an eukaryotic expression vector (pMAM) to construct pMLGT, which was expressed in the insulin-sensitive Chinese hamster ovary (CHO) cells. Western blot analysis showed that the GLUT2 gene in pMLGT was expressed in the transfected CHO cells successfully. The GLUT2 content in the plasma membrane fraction of insulin-treated CHO cells expressing GLUT2 increased 3.8-fold compared to that of the control group. This result suggests that GLUT2, which is not subjected to translocation by insulin in the cells of its major distribution, can be translocated if it is expressed in the suitable cells sensitive to insulin action.
journal_name
Yonsei Med Jjournal_title
Yonsei medical journalauthors
Ahn YH,Yoon DJ,Han GS,Lee BGdoi
10.3349/ymj.1993.34.2.117subject
Has Abstractpub_date
1993-06-01 00:00:00pages
117-25issue
2eissn
0513-5796issn
1976-2437journal_volume
34pub_type
杂志文章abstract:PURPOSE:We evaluated the validity of robotic surgery (RS) for pediatric choledochal cyst (CC) in comparison to open surgery (OS). MATERIALS AND METHODS:From January 2009 to April 2013, clinical data from 79 consecutive pediatric patients with CC, who underwent RS (n=36) or OS (n=43) performed by a single pediatric sur...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2015.56.3.737
更新日期:2015-05-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy of minimal stimulation using discretely administered gonadotropin combined with clomiphene citrate (CC) or letrozole (LTZ) for intrauterine insemination (IUI) cycles. MATERIALS AND METHODS:Total 257 IUI cycles from 158 infertile couples were assessed. A CC dose of 100 mg/day (n=126 cyc...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2015.56.2.490
更新日期:2015-03-01 00:00:00
abstract::Isotretinoin is a known human teratogen that can cause multiple malformations. At present, women who conceive one cycle after discontinuing isotretinoin are told that their teratogenic risk is not higher than baseline. We present a case of both-ear malformation in a newborn whose mother had taken isotretinoin for 2 ye...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.3.445
更新日期:2009-06-30 00:00:00
abstract::Previously, we reported that epigallocatechin 3-O-gallate (EGCg) has growth-inhibitory effect on clinical isolates of Candida species. In this study, we investigated the antifungal activity of EGCg and antifungal agents against thirty-five of dermatophytes clinically isolated by the international guidelines (M38-A2). ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2011.52.3.535
更新日期:2011-05-01 00:00:00
abstract::This study was performed to investigate the effects of behavior modification on body image, depression and body fat in obese elementary school children. Sixty-two elementary students of the 4th to 6th grade were selected from two different Seoul schools. Thirty-four children in one school were designated as the experi...
journal_title:Yonsei medical journal
pub_type: 临床试验,杂志文章
doi:10.3349/ymj.2004.45.1.61
更新日期:2004-02-29 00:00:00
abstract:PURPOSE:The effects of air cleaners on the removal of airborne indoor allergens, especially house dust mites (HDM), are still controversial. The objective of this study is to evaluate the effect of an air cleaner with an electrostatic filter on the removal of airborne mite allergens. MATERIALS AND METHODS:A dried HDM ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2010.51.6.918
更新日期:2010-11-01 00:00:00
abstract::Sarcoma of the kidney is a rare condition. Leiomyosarcoma is the most common of the kidney sarcomas. Renal leiomyosarcoma usually originates from the smooth muscle layers of the kidney, for example, the renal capsule and renal vessels. Renal pelvis neoplasms, however, are primarily transitional cell carcinomas, and re...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2007.48.3.557
更新日期:2007-06-30 00:00:00
abstract::Contrast-enhanced multi-detector row spiral computed tomography (MDCT) was introduced as a promising noninvasive method for vascular imaging. This study examined the accuracy of this technique for detecting significant coronary artery stenoses. Both MDCT(Sensation 16, Siemens, Germany, 12x0.75 mm collimation and 0.42 ...
journal_title:Yonsei medical journal
pub_type: 临床试验,杂志文章
doi:10.3349/ymj.2005.46.1.86
更新日期:2005-02-28 00:00:00
abstract::The purpose of this study was to determine factors that could predict the one-year response of the lumbar bone mineral density (BMD) to alendronate treatment in elderly Japanese women with osteoporosis. Eighty-five postmenopausal women with osteoporosis, all of whom were between 55-88 years of age, were treated with a...
journal_title:Yonsei medical journal
pub_type: 临床试验,杂志文章
doi:10.3349/ymj.2004.45.4.676
更新日期:2004-08-31 00:00:00
abstract:PURPOSE:Magnetocardiography (MCG) has been proposed as a noninvasive, diagnostic tool for risk-stratifying patients with acute myocardial infarction (AMI). This study evaluated whether MCG predicts long-term prognosis in AMI. MATERIALS AND METHODS:In 124 AMI patients (95 males, mean age 60±11 years), including 39 with...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2016.57.6.1339
更新日期:2016-11-01 00:00:00
abstract:PURPOSE:Evidence on the contribution of genes to the hereditary predisposition to pulmonary arterial hypertension (PAH) is limited. MATERIALS AND METHODS:In this study, we hypothesized that single nucleotide variants in vascular endothelial growth factor (VEGF) gene may alter gene function and expression and may be as...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2017.58.2.305
更新日期:2017-03-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is a dystrophinopathy, and its associated gene is located on Xp21. Moreover, utrophin, a recently identified structural homologue of dystrophin is reported to be up-regulated in DMD. In order to investigate the association between utrophin and muscle regeneration in DMD, an immunohist...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2003.44.1.15
更新日期:2003-02-01 00:00:00
abstract::The main purpose of this study was to describe the differences in health status between urban and rural elders in Korea. The sample consisted of one thousand one hundred non-institutionalized individuals, aged 65 or over, living in three metropolitan cities and two rural provinces. The modified Cornell Medical Index, ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1998.39.5.417
更新日期:1998-10-01 00:00:00
abstract::Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant muscular disorder characterized by weakness of facial, shoulder, abdominal, hip girdle, humeral, and anterior distal leg muscles, with descending progression from the face to the legs in an asymmetric pattern. In about 5% of patients with FSHD, no ...
journal_title:Yonsei medical journal
pub_type:
doi:10.3349/ymj.2021.62.1.95
更新日期:2021-01-01 00:00:00
abstract:PURPOSE:The current methods used to evaluate the efficacy of drug products are inadequate. We propose a non-inferiority approach to prove the safety of drugs. MATERIALS AND METHODS:Traditional hypotheses for the evaluation of the safety of drugs are based on proof of hazard, which have proven to be inadequate. Therefo...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2007.48.6.895
更新日期:2007-12-31 00:00:00
abstract::To determine whether the dysfunction of p53 protein, caused either by the mutation of p53 gene itself or by the human papillomavirus (HPVs) is involved in the development of cervical cancers and to find out the status of p53 tumor suppressor gene in HPV positive or negative cancers, we analyzed 64 cases of primary cer...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1995.36.5.412
更新日期:1995-11-01 00:00:00
abstract::We experienced a case of immunoglobulin E (IgE) mediated anaphylaxis to levodropropizine. The patient was an 18-year old Korean woman. After taking the common cold medication including acetaminophen, domperidone, and levodropropizine, skin rash, angioedema and anaphylaxis were developed immediately. As she was tolerab...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.1.262
更新日期:2013-01-01 00:00:00
abstract::In this paper, a case of corneal squamous cell carcinoma is reported. Invasive squamous cell carcinoma of the cornea is a rare disorder and has not been previously described in the Korean literature. In this case, the invasive squamous cell carcinoma of the cornea was treated by complete excision and cryotherapy. No e...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2001.42.5.576
更新日期:2001-10-01 00:00:00
abstract:PURPOSE:Several signaling pathways have been shown to regulate the lineage commitment and terminal differentiation of bone marrow stromal cells (BMSCs). Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis. In the present study, we tested the role of bone morphogenetic prote...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2010.51.5.740
更新日期:2010-09-01 00:00:00
abstract::In this study, the effects of chronically administered aluminum on iron metabolism-related parameters of liver and blood of mice were investigated. An additional purpose to determine how chronic aluminum administration together with vitamin E as an antioxidant to mice changed the parameters related to iron metabolism....
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2004.45.1.135
更新日期:2004-02-29 00:00:00
abstract::A few approaches can be used to decompress traumatic facial nerve paralysis including the middle cranial fossa approach or transmastoid approach depending on the site of injury. In some specific situation of treating traumatic facial nerve palsy whose injured site was confined from the geniculate ganglion to the secon...
journal_title:Yonsei medical journal
pub_type:
doi:10.3349/ymj.2018.59.3.457
更新日期:2018-05-01 00:00:00
abstract::The aim of this research was to estimate the community prevalence of alcoholism and the potential risk factors that affect it in the Edirne provincial centre by using a scanning test. A cross-sectional study was carried out in the Edirne provincial centre. A sample population composed of 500 women and 200 men was sele...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2004.45.2.207
更新日期:2004-04-30 00:00:00
abstract::A porcine heart valve was irradiated by Ultraviolet (UV) rays (10 W, 254 nm) for 2, 4, 8 and 24 hours at 4 degrees C to cross-link the structural collagen matrix. The degree of cross-linking was evaluated by assaying the released amount of hydroxyproline (Hyp) from the matrix, and comparing it with the positive contro...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1999.40.2.159
更新日期:1999-04-01 00:00:00
abstract::Telomerase reverse transcriptase (TERT) is the protein component of telomerase and combined with an RNA molecule, telomerase RNA component, forms the telomerase enzyme responsible for telomere elongation. Telomerase is essential for maintaining telomere length from replicative attrition and thus contributes to the pre...
journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
doi:10.3349/ymj.2014.55.1.1
更新日期:2014-01-01 00:00:00
abstract::When cells are exposed to a low dose of a mutagenic or clastogenic agent, they often become less sensitive to the effects of a higher dose administered subsequently. Such adaptive responses were first described in Escherichia coli. Studies on mammalian cells have been limited to human lymphocytes exposed to low doses ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1994.35.1.77
更新日期:1994-03-01 00:00:00
abstract::This corrects the article on p. 750 in vol. 46, PMID: 16385649. ...
journal_title:Yonsei medical journal
pub_type: 杂志文章,撤回出版物
doi:10.3349/ymj.2019.60.1.117
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:Long non-coding RNA taurine upregulated gene 1 (TUG1) is reported to be a vital regulator of the progression of various cancers. This study aimed to explore the exact roles and molecular mechanisms of TUG1 in osteosarcoma (OS) development. MATERIALS AND METHODS:Real-time quantitative PCR was applied to detect ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2018.59.2.226
更新日期:2018-03-01 00:00:00
abstract::The intestinal microbiota is a complex ecosystem consisting of various microorganisms that expands human genetic repertoire and therefore affects human health and disease. The metabolic processes and signal transduction pathways of the host and intestinal microorganisms are intimately linked, and abnormal progression ...
journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
doi:10.3349/ymj.2018.59.1.4
更新日期:2018-01-01 00:00:00
abstract::As the Korean government's recognition of the importance of hospice service grows, the government has initiated a variety of hospice services in Korea. Each hospice organization has shown a significant difference in its health care delivery methods, constitution and care content. Developing a clinical protocol is esse...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2005.46.1.8
更新日期:2005-02-28 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the efficacy of low-dose oral methotrexate (MTX) as a treatment for patients with Kawasaki disease (KD) which was resistant to intravenous immunoglobulin (IVIG). PATIENTS AND METHODS:The patients who had persistent or recrudescent fever after treatment with IVIG were subse...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2008.49.5.714
更新日期:2008-10-31 00:00:00