Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition.

Abstract:

:Nucleoplasmin (NP) is a pentameric histone chaperone that regulates the condensation state of chromatin in different cellular processes. We focus here on the interaction of NP with the histone octamer, showing that NP could bind sequentially the histone components to assemble an octamer-like particle, and crosslinked octamers with high affinity. The three-dimensional reconstruction of the NP/octamer complex generated by single-particle cryoelectron microscopy, revealed that several intrinsically disordered tail domains of two NP pentamers, facing each other through their distal face, encage the histone octamer in a nucleosome-like conformation and prevent its dissociation. Formation of this complex depended on post-translational modification and exposure of the acidic tract at the tail domain of NP. Finally, NP was capable of transferring the histone octamers to DNA in vitro, assembling nucleosomes. This activity may have biological relevance for processes in which the histone octamer must be rapidly removed from or deposited onto the DNA.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Franco A,Arranz R,Fernández-Rivero N,Velázquez-Campoy A,Martín-Benito J,Segura J,Prado A,Valpuesta JM,Muga A

doi

10.1038/s41598-019-45726-7

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

9487

issue

1

issn

2045-2322

pii

10.1038/s41598-019-45726-7

journal_volume

9

pub_type

杂志文章
  • Enduring rules of care within pairs - how blue tit parents resume provisioning behaviour after experimental disturbance.

    abstract::Sexual conflict over parental investment can result in suboptimal reproductive output. A recent hypothesis suggests that equality in investment, and hence conflict resolution, may be reached via coordination of parental activities like alternating nest visits. However, how robust patterns of care within couples are ag...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39139-9

    authors: Iserbyt A,Griffioen M,Eens M,Müller W

    更新日期:2019-02-26 00:00:00

  • Neandertal cannibalism and Neandertal bones used as tools in Northern Europe.

    abstract::Almost 150 years after the first identification of Neandertal skeletal material, the cognitive and symbolic abilities of these populations remain a subject of intense debate. We present 99 new Neandertal remains from the Troisième caverne of Goyet (Belgium) dated to 40,500-45,500 calBP. The remains were identified thr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29005

    authors: Rougier H,Crevecoeur I,Beauval C,Posth C,Flas D,Wißing C,Furtwängler A,Germonpré M,Gómez-Olivencia A,Semal P,van der Plicht J,Bocherens H,Krause J

    更新日期:2016-07-06 00:00:00

  • Efficacy of alprostadil for preventing of contrast-induced nephropathy: A meta-analysis.

    abstract::Contrast-induced nephropathy (CIN) has become the third-leading cause of hospital-acquired acute renal injury. Although alprostadil has been proposed as an effective preventative measure, this conclusion remains inconsistent. Thus, we performed a meta-analysis of the published studies on this topic to evaluate the pre...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-017-01160-1

    authors: Zhang JZ,Kang XJ,Gao Y,Zheng YY,Wu TT,Li L,Liu F,Yang YN,Li XM,Ma YT,Xie X

    更新日期:2017-04-21 00:00:00

  • 8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions.

    abstract::Oxidative stress and mitochondrial dysfunction are implicated in aging-related neurodegenerative disorders. 8-Oxoguanine (8-oxoG), a common oxidised base lesion, is often highly accumulated in brains from patients with neurodegenerative disorders. MTH1 hydrolyses 8-oxo-2'-deoxyguanosine triphosphate (8-oxo-dGTP) to 8-...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22086

    authors: Leon J,Sakumi K,Castillo E,Sheng Z,Oka S,Nakabeppu Y

    更新日期:2016-02-25 00:00:00

  • Relative abundance of chemical forms of Cu(II) and Cd(II) on soybean roots as influenced by pH, cations and organic acids.

    abstract::Little information is available on chemical forms of heavy metals on integrate plant roots. KNO3 (1 M), 0.05M EDTA at pH6 and 0.01 M HCl were used sequentially to extract the exchangeable, complexed and precipitated forms of Cu(II) and Cd(II) from soybean roots and then to investigate chemical form distribution of Cu(...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36373

    authors: Zhou Q,Liu ZD,Liu Y,Jiang J,Xu RK

    更新日期:2016-11-02 00:00:00

  • Optimized Spiral Metal-Gallium-Nitride Nanowire Cavity for Ultra-High Circular Dichroism Ultraviolet Lasing at Room Temperature.

    abstract::Circularly polarized laser sources with small footprints and high efficiencies can possess advanced functionalities in optical communication and biophotonic integrated systems. However, the conventional lasers with additional circular-polarization converters are bulky and hardly compatible with nanophotonic circuits, ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26578

    authors: Liao WC,Liao SW,Chen KJ,Hsiao YH,Chang SW,Kuo HC,Shih MH

    更新日期:2016-05-25 00:00:00

  • Correlative electron and fluorescence microscopy of magnetotactic bacteria in liquid: toward in vivo imaging.

    abstract::Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cell...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06854

    authors: Woehl TJ,Kashyap S,Firlar E,Perez-Gonzalez T,Faivre D,Trubitsyn D,Bazylinski DA,Prozorov T

    更新日期:2014-10-31 00:00:00

  • Life cycle assessment of polyphenols extraction processes from waste biomass.

    abstract::Waste biomass from forestry and wood processing industries is a source to obtain fine chemicals, and its processing is a good example of circular economy, but it generates secondary environmental impacts. The main objective of this study was to analyse the environmental performances of laboratory scale processes for p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70587-w

    authors: Barjoveanu G,Pătrăuțanu OA,Teodosiu C,Volf I

    更新日期:2020-08-12 00:00:00

  • Resolving the smell of wood - identification of odour-active compounds in Scots pine (Pinus sylvestris L.).

    abstract::Being one of the most common trees in forests, Pinus sylvestris L. is a frequently used raw material for wood products. Its specific odour is, however, mostly unresolved to date. Accordingly, we investigated Scots pine wood samples grown in Germany for their main odorant composition. We employed dedicated odorant anal...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26626-8

    authors: Schreiner L,Bauer P,Buettner A

    更新日期:2018-05-29 00:00:00

  • Parametric study on the geometrical parameters of a lab-on-a-chip platform with tilted planar electrodes for continuous dielectrophoretic manipulation of microparticles.

    abstract::Advances in lab-on-a-chip (LOC) devices have led to significant improvements in the on-chip manipulation, separation, sorting, and isolation of particles and cells. Among various LOC-based approaches such as inertia-based methods, acoustophoresis, and magnetophoresis, the planar-slanted-electrode dielectrophoresis (DE...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68699-4

    authors: Dalili A,Taatizadeh E,Tahmooressi H,Tasnim N,Rellstab-Sánchez PI,Shaunessy M,Najjaran H,Hoorfar M

    更新日期:2020-07-16 00:00:00

  • Early weaning increases aggression and stereotypic behaviour in cats.

    abstract::Behaviour problems are common in companion felines, and problematic behaviour may be a sign of chronic stress. In laboratory animals, early weaning increases the risk for aggression, anxiety, and stereotypic behaviour. However, very few studies have focused on early weaning in one of the world's most popular pets, the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11173-5

    authors: Ahola MK,Vapalahti K,Lohi H

    更新日期:2017-09-04 00:00:00

  • Health Benefits from Nature Experiences Depend on Dose.

    abstract::Nature within cities will have a central role in helping address key global public health challenges associated with urbanization. However, there is almost no guidance on how much or how frequently people need to engage with nature, and what types or characteristics of nature need to be incorporated in cities for the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28551

    authors: Shanahan DF,Bush R,Gaston KJ,Lin BB,Dean J,Barber E,Fuller RA

    更新日期:2016-06-23 00:00:00

  • Phytonutrient diet supplementation promotes beneficial Clostridia species and intestinal mucus secretion resulting in protection against enteric infection.

    abstract::Plant extracts, or phytonutrients, are used in traditional medicine practices as supplements to enhance the immune system and gain resistance to various infectious diseases and are used in animal production as health promoting feed additives. To date, there are no studies that have assessed their mechanism of action a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09253

    authors: Wlodarska M,Willing BP,Bravo DM,Finlay BB

    更新日期:2015-03-19 00:00:00

  • A quantum phase transition in a quantum external field: superposing two magnetic phases.

    abstract::We study an Ising chain undergoing a quantum phase transition in a quantum magnetic field. Such a field can be emulated by coupling the chain to a central spin initially in a superposition state. We show that--by adiabatically driving such a system--one can prepare a quantum superposition of any two ground states of t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00655

    authors: Rams MM,Zwolak M,Damski B

    更新日期:2012-01-01 00:00:00

  • Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model.

    abstract::Transplantation of cardiomyocytes derived from induced pluripotent stem cell (iPSC-CMs) is a promising approach for increasing functional CMs during end-stage heart failure. Although major histocompatibility complex (MHC) class I matching between donor cells and recipient could reduce acquired immune rejection, innate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47134-3

    authors: Nakamura Y,Miyagawa S,Yoshida S,Sasawatari S,Toyofuku T,Toda K,Sawa Y

    更新日期:2019-07-25 00:00:00

  • The Generalization of Auditory Accommodation to Altered Spectral Cues.

    abstract::The capacity of healthy adult listeners to accommodate to altered spectral cues to the source locations of broadband sounds has now been well documented. In recent years we have demonstrated that the degree and speed of accommodation are improved by using an integrated sensory-motor training protocol under anechoic co...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11981-9

    authors: Watson CJG,Carlile S,Kelly H,Balachandar K

    更新日期:2017-09-14 00:00:00

  • Significance of penile hypersensitivity in premature ejaculation.

    abstract::To assess the correlation between penile hypersensitivity and premature ejaculation (PE), a total of 420 consecutive subjects attending our andrologic clinic for suspected PE were enrolled. The entire cohort was asked to complete the self-report intravaginal ejaculation latency time (IELT) by stopwatch. According to t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09155-8

    authors: Guo L,Liu Y,Wang X,Yuan M,Yu Y,Zhang X,Zhao S

    更新日期:2017-09-05 00:00:00

  • Future N deposition and precipitation changes will be beneficial for the growth of Haloxylon ammodendron in Gurbantunggut Desert, northwest China.

    abstract::Evaluation of precipitation and nitrogen (N) deposition in desert ecosystems helps to elucidate the reaction of desert ecosystems to future environmental changes. An in-situ field experiment was established to examine the influence of a long-term enhanced precipitation and N deposition on the photosynthetic traits and...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37245-8

    authors: Zhao WQ,Lv XH,Li YG,Wang ZK,Zhang W,Zhuang L

    更新日期:2019-03-08 00:00:00

  • Optimal donor for severe aplastic anemia patient requiring allogeneic hematopoietic stem cell transplantation: A large-sample study from China.

    abstract::HLA-haploidentical hematopoietic stem cell transplantation (HSCT) may be an option for severe aplastic anemia (SAA) patients. However, to date, no large-sample studies have been performed to determine which types of SAA patients are suitable for HLA-haploidentical HSCT. We retrospectively studied 189 consecutive patie...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究

    doi:10.1038/s41598-018-20853-9

    authors: Zeng Y,Wang S,Wang J,Liu L,Su Y,Lu Z,Zhang X,Zhang Y,Zhong JF,Peng L,Liu Q,Lu Y,Gao L,Zhang X

    更新日期:2018-02-06 00:00:00

  • Quantifying the Effect of Anthropogenic Climate Change on Calcifying Plankton.

    abstract::Widely regarded as an imminent threat to our oceans, ocean acidification has been documented in all oceanic basins. Projected changes in seawater chemistry will have catastrophic biotic effects due to ocean acidification hindering biogenic carbonate production, which will in turn lead to substantial changes in marine ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58501-w

    authors: Fox L,Stukins S,Hill T,Miller CG

    更新日期:2020-01-31 00:00:00

  • Topographic heterogeneity effect on the accumulation of Fukushima-derived radiocesium on forest floor driven by biologically mediated processes.

    abstract::The accident at the Fukushima Daiichi nuclear power plant caused serious radiocesium ((137)Cs) contamination of forest ecosystems located in mountainous and hilly regions with steep terrain. To understand topographic effects on the redistribution and accumulation of (137)Cs on forest floor, we investigated the distrib...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06853

    authors: Koarashi J,Atarashi-Andoh M,Takeuchi E,Nishimura S

    更新日期:2014-10-31 00:00:00

  • The genetic variation in the R1a clade among the Ashkenazi Levites' Y chromosome.

    abstract::Approximately 300,000 men around the globe self-identify as Ashkenazi Levites, of whom two thirds were previously shown to descend from a single male. The paucity of whole Y-chromosome sequences precluded conclusive identification of this ancestor's age, geographic origin and migration patterns. Here, we report the va...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14761-7

    authors: Behar DM,Saag L,Karmin M,Gover MG,Wexler JD,Sanchez LF,Greenspan E,Kushniarevich A,Davydenko O,Sahakyan H,Yepiskoposyan L,Boattini A,Sarno S,Pagani L,Carmi S,Tzur S,Metspalu E,Bormans C,Skorecki K,Metspalu M,Roots

    更新日期:2017-11-02 00:00:00

  • Exploring the Inhibitory Mechanism of Approved Selective Norepinephrine Reuptake Inhibitors and Reboxetine Enantiomers by Molecular Dynamics Study.

    abstract::Selective norepinephrine reuptake inhibitors (sNRIs) provide an effective class of approved antipsychotics, whose inhibitory mechanism could facilitate the discovery of privileged scaffolds with enhanced drug efficacy. However, the crystal structure of human norepinephrine transporter (hNET) has not been determined ye...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26883

    authors: Zheng G,Xue W,Wang P,Yang F,Li B,Li X,Li Y,Yao X,Zhu F

    更新日期:2016-05-27 00:00:00

  • A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.

    abstract::Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propos...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23165

    authors: Wang J,Fan Z,Zhao Y,Song Y,Chu H,Song W,Song Y,Pan X,Sun Y,Li D

    更新日期:2016-03-17 00:00:00

  • Angiotensin type 1a receptor deficiency decreases amyloid β-protein generation and ameliorates brain amyloid pathology.

    abstract::Alzheimer's disease is characterized by neuronal loss and cerebral accumulation of amyloid-β protein (Aβ) and lowering the generation of Aβ is a pivotal approach in the strategy of Alzheimer's disease treatment. Midlife hypertension is a major risk factor for the future onset of sporadic Alzheimer's disease and the us...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12059

    authors: Liu J,Liu S,Matsumoto Y,Murakami S,Sugakawa Y,Kami A,Tanabe C,Maeda T,Michikawa M,Komano H,Zou K

    更新日期:2015-07-08 00:00:00

  • Dynamic markers based on blood perfusion fluctuations for selecting skin melanocytic lesions for biopsy.

    abstract::Skin malignant melanoma is a highly angiogenic cancer, necessitating early diagnosis for positive prognosis. The current diagnostic standard of biopsy and histological examination inevitably leads to many unnecessary invasive excisions. Here, we propose a non-invasive method of identification of melanoma based on bloo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12825

    authors: Lancaster G,Stefanovska A,Pesce M,Marco Vezzoni G,Loggini B,Pingitore R,Ghiara F,Barachini P,Cervadoro G,Romanelli M,Rossi M

    更新日期:2015-08-11 00:00:00

  • Three-dimensional HepaRG spheroids as a liver model to study human genotoxicity in vitro with the single cell gel electrophoresis assay.

    abstract::Many efforts have been made in the last 30 years to develop more relevant in vitro models to study genotoxic responses of drugs and environmental contaminants. While 2D HepaRG cells are one of the most promising models for liver toxicology, a switch to 3D cultures that integrate both in vivo architecture and cell-cell...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47114-7

    authors: Mandon M,Huet S,Dubreil E,Fessard V,Le Hégarat L

    更新日期:2019-07-22 00:00:00

  • Iodine accumulation of the liver in patients treated with amiodarone can be unmasked using material decomposition from multiphase spectral-detector CT.

    abstract::Amiodarone accumulates in the liver, where it increases x-ray attenuation due to its iodine content. We evaluated liver attenuation in patients treated and not treated with amiodarone using true-non-contrast (TNC) and virtual-non-contrast (VNC) images acquired with spectral-detector-CT (SDCT). 142 patients, of which 2...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-64002-7

    authors: Laukamp KR,Lennartz S,Hashmi A,Obmann M,Ho V,Große Hokamp N,Graner FP,Gilkeson R,Persigehl T,Gupta A,Ramaiya N

    更新日期:2020-04-24 00:00:00

  • Author Correction: Investigation on the interface between Li10GeP2S12 electrolyte and carbon conductive agents in all-solid-state lithium battery.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-28601-9

    authors: Yoon K,Kim JJ,Seong WM,Lee MH,Kang K

    更新日期:2018-07-04 00:00:00

  • High-density native-range species affects the invasive plant Chromolaena odorata more strongly than species from its invasive range.

    abstract::Invasive plant species often form dense mono-dominant stands in areas they have invaded, while having only sparse distribution in their native ranges, and the reasons behind this phenomenon are a key point of research in invasive species biology. Differences in species composition between native and invasive ranges ma...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16376-4

    authors: Zheng Y,Liao Z

    更新日期:2017-11-22 00:00:00