Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa.

abstract：:The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA compl...

journal_title：Journal of molecular biology

authors： Gerdes K,Helin K,Christensen OW,Løbner-Olesen A

更新日期：1988-09-05 00:00:00

abstract：:The coupling between the conformational properties of double-stranded DNA and its internal dynamics has been examined. The solution structures of the isomeric DNA oligomers d(GCGTACGC)(2) (UM) and d(CGCTAGCG)(2) (CTSYM) were determined with (1)H NMR spectroscopy by utilizing distance restraints from total relaxation m...

journal_title：Journal of molecular biology

authors： Isaacs RJ,Spielmann HP

更新日期：2001-03-23 00:00:00

abstract：:Site-directed mutagenesis has been used to change individual amino acids of the larvicidal 27,000 Mr delta-endotoxin of Bacillus thuringiensis var. israelensis. Basic and acidic residues have been systematically replaced by alanine, and the resulting mutant polypeptides analysed for cytolytic and larvicidal activity, ...

journal_title：Journal of molecular biology

authors： Ward ES,Ellar DJ,Chilcott CN

更新日期：1988-08-05 00:00:00

abstract：:Ricin is a potent plant toxin which acts by removing a specific adenine residue from the ribosome. The X-ray crystal structure of a new, tetragonal crystal form of the recombinant ricin A-chain diffracting to 1.8 A resolution has been determined via molecular replacement methods and refined to a crystallographic R-fac...

journal_title：Journal of molecular biology

authors： Weston SA,Tucker AD,Thatcher DR,Derbyshire DJ,Pauptit RA

更新日期：1994-12-09 00:00:00

abstract：:In humans, NKG2D is an activating receptor on natural killer (NK) cells and a costimulatory receptor on certain T cells and plays a central role in mediating immune responses in autoimmune diseases, infectious diseases, and cancer. Monoclonal antibodies that antagonize or agonize immune responses mediated by human NKG...

journal_title：Journal of molecular biology

authors： Kwong KY,Baskar S,Zhang H,Mackall CL,Rader C

更新日期：2008-12-31 00:00:00

abstract：:We have applied a search strategy for determining the optimal packing of protein secondary structure elements to the rotational positioning of the seven transmembrane helices of bacteriorhodopsin. The search is based on the assumption that the relative orientations of the helices within the bundle are conditioned prin...

journal_title：Journal of molecular biology

authors： Tuffery P,Etchebest C,Popot JL,Lavery R

更新日期：1994-03-04 00:00:00

abstract：:A homologue of the Escherichia coli GroEL apical domain was obtained from thermophilic eubacterium Thermus thermophilus. The domains share 70 % sequence identity (101 out of 145 residues). The thermal stability of the T. thermophilus apical domain (Tm>100 degrees C as evaluated by circular dichroism) is at least 35 de...

journal_title：Journal of molecular biology

authors： Hua Q,Dementieva IS,Walsh MA,Hallenga K,Weiss MA,Joachimiak A

更新日期：2001-02-23 00:00:00

abstract：:Pathogenic strains of Helicobacter pylori use a type IV secretion system (T4SS) to deliver the toxin CagA into human host cells. The T4SS, along with the toxin itself, is coded into a genomic insert, which is termed the cag pathogenicity island. The cag pathogenicity island contains about 30 open-reading frames, for m...

journal_title：Journal of molecular biology

authors： Cendron L,Couturier M,Angelini A,Barison N,Stein M,Zanotti G

更新日期：2009-02-13 00:00:00

abstract：:By binding to the DNA site OE at position -60.5 in the gal operon, the GalR protein activates transcription from the P2 promoter located on the opposite face of DNA (position -5) and represses transcription from the P1 promoter located on the same face (position +1). GalR increases RNA polymerase binding at P2 and inh...

journal_title：Journal of molecular biology

authors： Choy HE,Hanger RR,Aki T,Mahoney M,Murakami K,Ishihama A,Adhya S

更新日期：1997-09-26 00:00:00

abstract：:Autonomously replicating sequences (ARSs) in the yeast Yarrowia lipolytica require two components: an origin of replication (ORI) and centromere (CEN) DNA, both of which are necessary for extrachromosomal maintenance. To investigate this cooperation in more detail, we performed a screen for genomic sequences able to c...

journal_title：Journal of molecular biology

authors： Vernis L,Poljak L,Chasles M,Uchida K,Casarégola S,Käs E,Matsuoka M,Gaillardin C,Fournier P

更新日期：2001-01-12 00:00:00

abstract：:The structure of recombinant human interleukin-4 (hIL-4) has been determined by both NMR and X-ray diffraction methods in several laboratories, including ours. The X-ray and NMR structures were successfully applied for solving the X-ray crystal structure by molecular replacement. Due to the small size of the hIL-4 mol...

journal_title：Journal of molecular biology

authors： Müller T,Oehlenschläger F,Buehner M

更新日期：1995-03-24 00:00:00

abstract：:A common feature of many of the most important and prominent amyloid-forming proteins is their ability to bind lipids and lipid complexes. Lipids are ubiquitous components of disease-associated amyloid plaques and deposits in humans, yet the specific roles of lipid in the process of amyloid fibril formation are poorly...

journal_title：Journal of molecular biology

authors： Griffin MD,Mok ML,Wilson LM,Pham CL,Waddington LJ,Perugini MA,Howlett GJ

更新日期：2008-01-04 00:00:00

abstract：:Steroids and retinoids are signaling molecules that control a variety of physiological processes. 17beta-Hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reduction of estrone to estradiol, supplying biologically active estrogen-regulating sex-specific differentiation. Photoreceptor-associated retinol de...

journal_title：Journal of molecular biology

authors： Haller F,Moman E,Hartmann RW,Adamski J,Mindnich R

更新日期：2010-06-04 00:00:00

abstract：:Catalase-1, one of four catalase activities of Neurospora crassa, is associated with non-growing cells and accumulates in asexual spores. It is a large, tetrameric, highly efficient, and durable enzyme that is active even at molar concentrations of hydrogen peroxide. Catalase-1 is oxidized at the heme by singlet oxyge...

journal_title：Journal of molecular biology

authors： Díaz A,Horjales E,Rudiño-Piñera E,Arreola R,Hansberg W

更新日期：2004-09-17 00:00:00

abstract：:Angiotensin I-converting enzyme (ACE), one of the central components of the renin-angiotensin system, is a key therapeutic target for the treatment of hypertension and cardiovascular disorders. Human somatic ACE (sACE) has two homologous domains (N and C). The N- and C-domain catalytic sites have different activities ...

journal_title：Journal of molecular biology

authors： Akif M,Georgiadis D,Mahajan A,Dive V,Sturrock ED,Isaac RE,Acharya KR

更新日期：2010-07-16 00:00:00

abstract：:A Rhizobium meliloti DNA region (nodD1) involved in the regulation of other early nodulation genes has been delimited by directed Tn5 mutagenesis and its nucleotide sequence has been determined. The sequence data indicate a large open reading frame with opposite polarity to nodA, -B and -C, coding for a protein of 308...

journal_title：Journal of molecular biology

authors： Göttfert M,Horvath B,Kondorosi E,Putnoky P,Rodriguez-Quiñones F,Kondorosi A

更新日期：1986-10-05 00:00:00

abstract：:We describe multi-conformation simulated annealing-pseudo-crystallographic refinement (MCSA-PCR), a technique developed for predicting the binding mode of a flexible ligand in a flexible binding pocket. To circumvent the local-minimum problem efficiently, this method performs multiple independent cycles of simulated a...

journal_title：Journal of molecular biology

authors： Ota N,Agard DA

更新日期：2001-11-30 00:00:00

abstract：:We report the sequence of a cDNA clone that encodes the C-terminal half of the hamster 94 X 10(3) Mr glucose-regulated protein, grp94. The amino acid sequence of this protein is about 50% homologous to Drosophila hsp83 and yeast hsp90, suggesting that grp94 and hsp90 have similar functional properties. Unlike hsp90, g...

journal_title：Journal of molecular biology

authors： Sorger PK,Pelham HR

更新日期：1987-03-20 00:00:00

abstract：:RNA polymerase II encounters various obstacles to transcript elongation both in vivo and in vitro. These include DNA sequence elements and protein bound to the major groove of DNA. Elongation factor SII binds to RNA polymerase II and enables the enzyme to bypass these impediments. SII also activates nascent RNA cleava...

journal_title：Journal of molecular biology

authors： Mote J Jr,Ghanouni P,Reines D

更新日期：1994-02-25 00:00:00

abstract：:The Cip/Kip family of cyclin-dependent kinase (Cdk) inhibitors includes p21(Cip1), p27(Kip1) and p57(Kip2). Their kinase inhibitory activities are mediated by a homologous N-terminal kinase inhibitory domain. The Cdk inhibitory activity and stability of p27 have been shown to be regulated by a two-step phosphorylation...

journal_title：Journal of molecular biology

authors： Huang Y,Yoon MK,Otieno S,Lelli M,Kriwacki RW

更新日期：2015-01-30 00:00:00

abstract：:We examined the effects of neutral salts and the non-ionic solute 2-methyl,-2,4-pentanediol (MPD) on transcript elongation by Escherichia coli RNA polymerase and on pausing induced by the multipartite his leader pause signal. All solutes tested slowed the overall rate of elongation, with anions showing the dominant ef...

journal_title：Journal of molecular biology

authors： Chan CL,Landick R

更新日期：1997-04-25 00:00:00

abstract：:YibK is a 160 residue homodimeric protein belonging to the SPOUT class of methyltransferases. Proteins in this group all display a unique topological feature; the backbone polypeptide chain folds to form a deep trefoil knot. Such knotted structures were completely unpredicted, it being thought impossible for a protein...

journal_title：Journal of molecular biology

authors： Mallam AL,Jackson SE

更新日期：2005-03-11 00:00:00

abstract：:The DnaB protein is the primary replicative helicase in Escherichia coli, and the active form of the protein is a hexamer. It has been reported that the protein forms a ring with strong 3-fold symmetry, which was suggested to be a trimer of dimers. We show that under different conditions, using either ATP, ATP gamma S...

journal_title：Journal of molecular biology

authors： Yu X,Jezewska MJ,Bujalowski W,Egelman EH

更新日期：1996-05-31 00:00:00

abstract：:Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis proposed to form specific trimer-of-trimers structures, acts as a molecular chaperone in vitro. The assembly and re-assembly mechanisms of this oligomeric protein were studied and compared using in vitro transcription/translation and denaturization/re...

journal_title：Journal of molecular biology

authors： Abulimiti A,Fu X,Gu L,Feng X,Chang Z

更新日期：2003-02-28 00:00:00

abstract：:Bombyxin-II, a brain-secretory peptide of the silkmoth Bombyx mori, shares 40% sequence identify and the characteristics core structure with human insulin. In spite of the structural similarity, no cross-activity is observed between them. To localize the active region of bombyxin-II, we have synthesized chimeric molec...

journal_title：Journal of molecular biology

authors： Nagata K,Hatanaka H,Kohda D,Kataoka H,Nagasawa H,Isogai A,Ishizaki H,Suzuki A,Inagaki F

更新日期：1995-11-10 00:00:00

abstract：:Many proteins are composed of independently-folded domains connected by flexible linkers. The primary sequence and length of such linkers can set the effective concentration for the tethered domains, which impacts rates of association and enzyme activity. The length of such linkers can be sensitive to environmental co...

journal_title：Journal of molecular biology

authors： Basak S,Sakia N,Dougherty L,Guo Z,Wu F,Mindlin F,Lary JW,Cole JL,Ding F,Bowen ME

更新日期：2021-01-01 00:00:00

abstract：:The tyrosyl-tRNA synthetases from Bacillus stearothermophilus (Bst-TyrTS) and Escherichia coli (Eco-TyrTS) are 56% identical in amino acid sequence. To map and characterize the set of interactions that makes Bst-TyrTS more stable than Eco-TyrTS, a family of nine hybrid proteins was constructed between the two enzymes....

journal_title：Journal of molecular biology

authors： Guez-Ivanier V,Hermann M,Baldwin D,Bedouelle H

更新日期：1993-11-05 00:00:00

abstract：:In order to determine how much of the natural sequence is required for function of the lambda tR1 rho-dependent terminator, and to determine the minimum length required, we made two deletion series of a tR1 derivative that contains mostly foreign DNA, encoding C-rich RNA, substituted for the natural upstream sequences...

journal_title：Journal of molecular biology

authors： Hart CM,Roberts JW

更新日期：1994-04-01 00:00:00

abstract：:We present evidence for translational activation of the Qbeta coliphage maturation cistron, mediated by the presence of Qbeta replicase. This activation does not require RNA replication, translation of a second gene, or any direct protein-RNA binding at the maturation gene initiation site. Our data support a model in ...

journal_title：Journal of molecular biology

authors： Priano C,Arora R,Jayant L,Mills DR

更新日期：1997-08-22 00:00:00

abstract：:Aminoacylation and editing by leucyl-tRNA synthetases (LeuRS) require migration of the tRNA acceptor stem end between the canonical aminoacylation core and a separate domain called CP1 that is responsible for amino acid editing. The LeuRS CP1 domain can also support group I intron RNA splicing in the yeast mitochondri...

journal_title：Journal of molecular biology

authors： Nawaz MH,Pang YL,Martinis SA

更新日期：2007-03-23 00:00:00