Abstract:
AIMS:Iron overload is a major factor contributing to the overall pathology of thalassaemia, which is primarily mediated by ineffective erythropoiesis and shorter mature red blood cell (RBC) survival. Iron accumulation in RBCs generates reactive oxygen species (ROS) that cause cellular damage such as lipid peroxidation and RBC membrane deformation. Abnormal RBCs in patients with thalassaemia are commonly known as microcytic hypochromic anaemia with poikilocytosis. However, iron and ROS accumulation in RBCs as related to RBC morphological changes in patients with thalassaemia has not been reported. METHODS:Twenty-one patients with thalassaemia, including HbH, HbH with Hb Constant Spring and β-thalassaemia/HbE (splenectomy and non-splenectomy) genotypes, and five normal subjects were recruited. RBC morphology was analysed by light and scanning electron microscopy. Systemic and RBC iron status and oxidative stress were examined. RESULTS:Decreased normocytes were observed in the samples of patients with thalassaemia, with RBC morphological abnormality being related to the type of disease (α-thalassaemia or β-thalassaemia) and splenic status. Target cells and crenated cells were mainly found in splenectomised patients with β-thalassaemia/HbE, while target cells and teardrop cells were found in non-splenectomised patients. Patients with thalassaemia had high levels of serum ferritin, red cell ferritin and ROS in RBCs compared with normal subjects (p<0.05). Negative correlations between the amount of normocytes and serum ferritin (rs=-0.518, p=0.011), red cell ferritin (rs=-0.467, p=0.025) or ROS in RBCs (rs=-0.672, p<0.001) were observed. CONCLUSIONS:Iron overload and its consequent intracellular oxidative stress in RBCs were associated with reduce normocytes in patients with thalassaemia.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Chaichompoo P,Qillah A,Sirankapracha P,Kaewchuchuen J,Rimthong P,Paiboonsukwong K,Fucharoen S,Svasti S,Worawichawong Sdoi
10.1136/jclinpath-2019-205775subject
Has Abstractpub_date
2019-08-01 00:00:00pages
520-524issue
8eissn
0021-9746issn
1472-4146pii
jclinpath-2019-205775journal_volume
72pub_type
杂志文章abstract:BACKGROUND:Receptor activator of nuclear factor kappabeta ligand (RANKL) has an important role in bone remodelling, and tumour necrosis factor related, apoptosis inducing ligand (TRAIL) can induce apoptosis in cancer cells. Their functions are linked by their interactions with osteoprotegerin (OPG). OBJECTIVE:To inves...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.030031
更新日期:2006-07-01 00:00:00
abstract::An investigation into the noise levels in a clinical chemistry laboratory has been carried out. It was shown that although the levels were insufficient to cause damage to hearing, they exceeded the criteria of acceptability for offices and workshops. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.5.445
更新日期:1970-07-01 00:00:00
abstract::The distribution and quantitative expression of HBcAg in relation to serum HBeAg and liver histology before and after a trial of interferon in 50 patients with chronic type B hepatitis were evaluated using polyclonal and monoclonal antibodies. In general, both antisera showed a similar pattern in terms of the distribu...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.44.1.21
更新日期:1991-01-01 00:00:00
abstract:AIM:To determine the prognostic significance and clinical relevance of cathepsin D detected by immunocytochemical assays (ICAs) in breast carcinomas. METHODS:151 patients presenting with palpable or impalpable breast carcinomas and who had not received any kind of adjuvant chemotherapy or endocrine therapy who were op...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.7.586
更新日期:1997-07-01 00:00:00
abstract::In a twenty-year period 19 appendicectomy specimens were diagnosed as primary Crohn's disease. This represents 4.9% of the total number of both resection specimens and mucosal biopsies diagnosed as Crohn's disease during that time. This is a review of the main histopathological features found in these appendices and t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.6.632
更新日期:1983-06-01 00:00:00
abstract:BACKGROUND:Although Bulgaria has one of the highest rates of cervical cancer incidence and mortality in the EU, data on HPV type distribution are limited. AIM:To determine the prevalence of HPV types 16 and 18 in archival specimens from cervical cancer cases from Bulgaria. METHODS:A total of 145 formalin-fixed, paraf...
journal_title:Journal of clinical pathology
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更新日期:2010-12-01 00:00:00
abstract:AIM:To assess the reliability of local international normalised ratios (INR) using a set of three international reference preparation (IRP) certified freeze dried plasmas. METHODS:55 centres in the United Kingdom and the Republic of Ireland participated; 36 centres employed coagulometers and 19 a manual prothrombin ti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
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更新日期:1998-05-01 00:00:00
abstract::Of the 123 patients with acute mesenteric infarction treated over the past 12 years, 16 (13%) had mesenteric venous thrombosis. Eight of the patients with mesenteric venous thrombosis survived the initial episode; two have since died. The remaining six patients were studied for evidence of haemostatic deficiencies or ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.8.906
更新日期:1987-08-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.8.630
更新日期:1990-08-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.9.918
更新日期:1979-09-01 00:00:00
abstract:AIMS:Caucasian patients with disorders of sex development (DSD) are at a high risk of developing germ cell cancer (GCC). GCC is prominent in young adults in Western countries, while the incidence is significantly lower in Asia. So far, the risk of GCC in Asian DSD patients is unknown. METHODS AND RESULTS:A detailed st...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-201062
更新日期:2013-03-01 00:00:00
abstract::Accurate diagnosis of Clostridium difficile infection (CDI) is essential for optimal treatment, prevention and control. There are two reference assays for CDI diagnosis: the cell cytotoxicity assay (CCTA) and toxigenic culture (TC). Importantly, these tests actually detect different targets: CCTA detects the presence ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.080135
更新日期:2011-01-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200096
更新日期:2011-10-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.9.713
更新日期:2001-09-01 00:00:00
abstract::Inhibition of streptolysin O activity by cholesterol depends on the latter being in free form. The normal esterified and protein-bound cholesterol fractions in serum do not influence streptolysin O activity. However, high cholesterol levels in rabbits fed cholesterol with cholic acid were associated with an increased ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.10.885
更新日期:1972-10-01 00:00:00
abstract::The sensitivity of methods to detect antibodies to intrinsic factor was assessed. Five sera of known antibody content were tested in 31 laboratories and 30 sera from patients with pernicious anaemia were tested in one laboratory. Five non-commercial methods and two kits for type I antibodies and one non-commercial met...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.2.210
更新日期:1989-02-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.6.487
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.1.44
更新日期:1983-01-01 00:00:00
abstract::Two cases are described in which clostridial invasion of the liver and spleen was noted at post-mortem examination. In each there was a history of debilitating disease and it is suggested that the infection was consequent upon impaired immunological and cellular defence mechanisms. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.2.170
更新日期:1965-03-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.11.1051
更新日期:1995-11-01 00:00:00
abstract::MUCs are glycoproteins with various roles in homeostasis and carcinogenesis. Among other actions, MUC1 may inhibit cell-cell and cell-stroma interactions and function as a signal transducer, participating in cancer progression. In contrast, MUC2 is normally found only in goblet cells, where it contributes to the prote...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2003.013292
更新日期:2004-05-01 00:00:00
abstract::Factor VIII procoagulant activity (VIII:C), factor VIII related antigen (VIIIR:AG), and the von Willebrand factor (VIII:WF) were measured in 19 patients with liver disease (8 cases of alcoholic cirrhosis and 11 with acute viral hepatitis). In both groups of patients the levels of VIII:C, VIIIR:AG, and VIIIR:WF were ab...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.3.221
更新日期:1977-03-01 00:00:00
abstract:BACKGROUND:Malignant deciduoid mesothelioma is a rare variant of epithelioid mesothelioma. This tumour generally has poor prognosis, and can be asbestos related. AIM:To identify peculiar genetic changes responsible for critical phases in pathogenesis of malignant deciduoid mesothelioma and their prognostic relevance. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026435
更新日期:2006-07-01 00:00:00
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journal_title:Journal of clinical pathology
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更新日期:1995-08-01 00:00:00
abstract::A manual system of microbiology reporting with a National Cash Register (NCR) form with printed names of bacteria and antiboitics required less time to compose reports than a previous manual system that involved rubber stamps and handwriting on plain report sheets. The NCR report cost 10-28 pence and, compared with a ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.6.553
更新日期:1976-06-01 00:00:00
abstract::Human papillomavirus antigen was found in 39 (16%) of 253 colposcopic biopsies from a group of women at high risk for cervical cancer who had been examined in the early 1970s. Immunohistochemical evidence of papillomavirus infection was found in 20 (30%) of 67 cervical intraepithelial neoplasia lesions infected with w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.8.855
更新日期:1985-08-01 00:00:00