Abstract:
BACKGROUND:Previously, we could show that L-lactate affects cultured bovine granulosa cells (GC) in a specific manner driving the cells into an early pre-ovulatory phenotype. Here we studied genome wide effects in L-lactate-treated GC to further elucidate the underlying mechanisms that are responsible for the L-lactate induced transformation. Cultured estrogen producing GC treated either with L-lactate or vehicle control were subjected to mRNA microarray analysis. RESULTS:The analysis revealed 487 differentially expressed clusters, representing 461 annotated genes. Of these, 333 (= 318 genes) were identified as up- and 154 (= 143 genes) as down-regulated. As the top up-regulated genes we detected TXNIP, H19 and AHSG as well as our previously established marker transcripts RGS2 and PTX3. The top down-regulated genes included VNN1, SLC27A2 and GFRA1, but also MYC and the GC marker transcript CYP19A1. Pathway analysis with differentially expressed genes indicated "cAMP-mediated signaling" and "Axon guidance signaling" among the most affected pathways. Furthermore, estradiol, progesterone and Vegf were identified as potential upstream regulators. An effector network analysis by IPA provided first hints that processes of "angiogenesis" and "vascularization", but also "cell movement" appeared to be activated, whereas "organismal death" was predicted to be inhibited. CONCLUSIONS:Our data clearly show that L-lactate alters gene expression in cultured bovine GC in a broad, but obviously specific manner. Pathway analysis revealed that the mode of L-lactate action in GC initiates angiogenic processes, but also migratory events like cell movement and axonal guidance signaling, thus supporting the transformation of GC into an early luteal phenotype.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Baufeld A,Koczan D,Vanselow Jdoi
10.1186/s12864-019-5657-6subject
Has Abstractpub_date
2019-04-05 00:00:00pages
273issue
1issn
1471-2164pii
10.1186/s12864-019-5657-6journal_volume
20pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:Fragile sites are the chromosomal regions that are susceptible to breakage, and their frequency varies among the human population. Based on the frequency of fragile site induction, they are categorized as common and rare fragile sites. Common fragile sites are sensitive to replication stress and often rearra...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5330-5
更新日期:2019-04-18 00:00:00
abstract:BACKGROUND:A fundamental requirement for genomic studies is the availability of genetic material of good quality and quantity. The desired quantity and quality are often hard to obtain when target DNA is composed of complex mixtures of relatively short DNA fragments. Here, we sought to develop a method to representativ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-415
更新日期:2008-09-15 00:00:00
abstract:BACKGROUND:Shotgun sequencing of environmental DNA is an essential technique for characterizing uncultivated microbes in situ. However, the taxonomic and functional assignment of the obtained sequence fragments remains a pressing problem. RESULTS:Existing algorithms are largely optimized for speed and coverage; in con...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-461
更新日期:2010-08-05 00:00:00
abstract:BACKGROUND:Aedes aegypti is the principle vector of many arboviruses, including dengue virus and Zika virus, which are transmitted when an infected female mosquito takes a blood meal in order to initiate vitellogenesis. During blood digestion, ~ 10 mM heme-iron is ingested into the midgut lumen. While heme acts as both...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06981-5
更新日期:2020-08-31 00:00:00
abstract:BACKGROUND:The transition from exponential to stationary phase in Streptomyces coelicolor is accompanied by a major metabolic switch and results in a strong activation of secondary metabolism. Here we have explored the underlying reorganization of the metabolome by combining computational predictions based on constrain...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-202
更新日期:2010-03-26 00:00:00
abstract:BACKGROUND:Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-2047-6
更新日期:2015-11-05 00:00:00
abstract:BACKGROUND:The genetic mechanisms underlying hemangioblastoma development are still largely unknown. We used high-resolution single nucleotide polymorphism microarrays and droplet digital PCR analysis to detect copy number variations (CNVs) in total of 45 hemangioblastoma tumors. RESULTS:We identified 94 CNVs with a m...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2370-6
更新日期:2016-01-14 00:00:00
abstract:BACKGROUND:Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray to analyze the gene transcription profile of the lepidopteran pest Spodoptera frugiperda in response to injection of the polydnavirus HdIV associated with the ichneumonid wasp...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-7-160
更新日期:2006-06-21 00:00:00
abstract:BACKGROUND:Advances in whole genome profiling have revolutionized the cancer research field, but at the same time have raised new bioinformatics challenges. For next generation sequencing (NGS), these include data storage, computational costs, sequence processing and alignment, delineating appropriate statistical measu...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S6-S14
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-27
更新日期:2002-09-27 00:00:00
abstract:BACKGROUND:Phenomics provides new technologies and platforms as a systematic phenome-genome approach. However, few studies have reported on the systematic mining of shared genetics among clinical biochemical indices based on phenomics methods, especially in China. This study aimed to apply phenomics to systematically e...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6363-0
更新日期:2019-12-16 00:00:00
abstract:BACKGROUND:Transcription factor (TF) GAMYB, belonging to MYB family (named after the gene of the avian myeloblastosis virus) is a master gibberellin (GA)-induced regulatory protein that is crucial for development and germination of cereal grain and involved in anther formation. It activates many genes including high-mo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06991-3
更新日期:2020-08-24 00:00:00
abstract:BACKGROUND:'Systems-wide' approaches such as microarray RNA-profiling are ideally suited to the study of the complex overlapping responses of plants to biotic and abiotic stresses. However, commercial microarrays are only available for a limited number of plant species and development costs are so substantial as to be ...
journal_title:BMC genomics
pub_type: 杂志文章,meta分析
doi:10.1186/1471-2164-10-436
更新日期:2009-09-16 00:00:00
abstract:BACKGROUND:Oxidative stress is a consequence of normal and abnormal cellular metabolism and is linked to the development of human diseases. The effective functioning of the pathway responding to oxidative stress protects the cellular DNA against oxidative damage; conversely the failure of the oxidative stress response ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S6-S4
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Initial interest in Dechloromonas aromatica strain RCB arose from its ability to anaerobically degrade benzene. It is also able to reduce perchlorate and oxidize chlorobenzoate, toluene, and xylene, creating interest in using this organism for bioremediation. Little physiological data has been published for ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-351
更新日期:2009-08-03 00:00:00
abstract:BACKGROUND:Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially redu...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2612-7
更新日期:2016-04-18 00:00:00
abstract:BACKGROUND:Whole exome sequencing studies identify hundreds to thousands of rare protein coding variants of ambiguous significance for human health. Computational tools are needed to accelerate the identification of specific variants and genes that contribute to human disease. RESULTS:We have developed the Variant Eff...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-S3-S3
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) post-transcriptionally regulate a variety of genes involved in eukaryotic cell growth, development, metabolism and other biological processes, and numerous miRNAs are implicated in the initiation and progression of cancer. Enzootic nasal adenocarcinoma (ENA), an epithelial tumor induced in...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3238-5
更新日期:2016-11-08 00:00:00
abstract:BACKGROUND:Genome rearrangement describes gross changes of chromosomal regions, plays an important role in evolutionary biology and has profound impacts on phenotype in organisms ranging from microbes to humans. With more and more complete genomes accomplished, lots of genomic comparisons have been conducted in order t...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3655-0
更新日期:2017-03-29 00:00:00
abstract:BACKGROUND:Intensive research based on the inverse expression relationship has been undertaken to discover the miRNA-mRNA regulatory modules involved in the infection of Hepatitis C virus (HCV), the leading cause of chronic liver diseases. However, biological studies in other fields have found that inverse expression r...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-16-S2-S11
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Association mapping approaches are dependent upon discovery and validation of single nucleotide polymorphisms (SNPs). To further association studies in Anopheles gambiae we conducted a major resequencing programme, primarily targeting regions within or close to candidate genes for insecticide resistance. RE...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-320
更新日期:2009-07-16 00:00:00
abstract:BACKGROUND:Peromyscus are the most common mammalian species in North America and are widely used in both laboratory and field studies. The deer mouse, P. maniculatus and the old-field mouse, P. polionotus, are closely related and can generate viable and fertile hybrid offspring. The ability to generate hybrid offspring...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06969-1
更新日期:2020-09-11 00:00:00
abstract:BACKGROUND:We use an approach based on Factor Analysis to analyze datasets generated for transcriptional profiling. The method groups samples into biologically relevant categories, and enables the identification of genes and pathways most significantly associated to each phenotypic group, while allowing for the partici...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-6-109
更新日期:2005-08-17 00:00:00
abstract:BACKGROUND:Most of our understanding on the social behavior and genomics of bees and other social insects is centered on the Western honey bee, Apis mellifera. The genus Apis, however, is a highly derived branch comprising less than a dozen species, four of which genomically characterized. In contrast, for the equally ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06784-8
更新日期:2020-06-03 00:00:00
abstract:BACKGROUND:Bacteriophages that infect the opportunistic pathogen Pseudomonas aeruginosa have been classified into several groups. One of them, which includes temperate phage particles with icosahedral heads and long flexible tails, bears genomes whose architecture and replication mechanism, but not their nucleotide seq...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-1146
更新日期:2014-12-19 00:00:00
abstract:BACKGROUND:Using a combination of pyrosequencing and conventional Sanger sequencing, the complete genome sequence of the recently described novel Brucella species, Brucella microti, was determined. B. microti is a member of the genus Brucella within the Alphaproteobacteria, which consists of medically important highly ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-352
更新日期:2009-08-04 00:00:00
abstract:BACKGROUND:Codon usage analysis has been a classical topic for decades and has significances for studies of evolution, mRNA translation, and new gene discovery, etc. While the codon usage varies among different members of the plant kingdom, indicating the necessity for species-specific study, this work has mostly been ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-732
更新日期:2013-10-25 00:00:00
abstract:BACKGROUND:About 6 million Americans suffer from heart failure and 70% of heart failure cases are caused by myocardial infarction (MI). Following myocardial infarction, increased cytokines induce two major types of macrophages: classically activated macrophages which contribute to extracellular matrix destruction and a...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S6-S21
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Toxin-antitoxin (TA) systems, abundant in prokaryotes, are composed of a toxin gene and its cognate antitoxin. Several toxins are implied to affect the physiological state and stress tolerance of bacteria in a population. We previously identified a chromosomally encoded hok-sok type I TA system in Erwinia am...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-021-07376-w
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:Wild sugarcane Saccharum spontaneum plants vary in ploidy, which complicates the utilization of its germplasm in sugarcane breeding. Investigations on cold tolerance in relation to different ploidies in S. spontaneum may promote the exploitation of its germplasm and accelerate the improvement of sugarcane va...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06917-z
更新日期:2020-07-22 00:00:00