The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.

Abstract:

BACKGROUND:Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to cold stress in this study. METHODS:The second to fourth fully expanded leaves from up to down were harvested at different stress time points forthe transmission electron microscope (TEM) observation. Physiological characteristics measurement included the relative electrolyte leakage (REL), SOD activity assay, soluble sugar content, and Chlorophyll fluorescence parameter measurement. For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads. To identify the differentially expressed protein, leaves of plants under 0, 6, 12, 24, 48 and 72 h cold stress wereharvested for proteomic analysis. Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data. RESULTS:Results showed that at the beginning of cold stress, respiratory metabolism was decreased and the transportation and hydrolysis of photosynthetic products was inhibited, leading to an accumulation of starch in the chloroplasts. Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism. Although the transcriptional level of a large number of genes has been restored after 12 h, sustained cold stress brought more serious injury to the leaf cells, including the sharp rise of the relative electrolyte leakage, the declined Fv/Fm value, swelled chloroplast and the disintegrated membrane system. CONCLUSION:The starch accumulation and the photoinhibition might be the main adaptive mechanism of the paper mulberry responded to cold stress. Most of important, enhancing the transport and hydrolysis of photosynthetic products could be the potential targets for improving the cold tolerance of the paper mulberry.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Peng X,Teng L,Yan X,Zhao M,Shen S

doi

10.1186/s12864-015-2047-6

subject

Has Abstract

pub_date

2015-11-05 00:00:00

pages

898

issn

1471-2164

pii

10.1186/s12864-015-2047-6

journal_volume

16

pub_type

杂志文章
  • Construction of relatedness matrices using genotyping-by-sequencing data.

    abstract:BACKGROUND:Genotyping-by-sequencing (GBS) is becoming an attractive alternative to array-based methods for genotyping individuals for a large number of single nucleotide polymorphisms (SNPs). Costs can be lowered by reducing the mean sequencing depth, but this results in genotype calls of lower quality. A common analys...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-015-2252-3

    authors: Dodds KG,McEwan JC,Brauning R,Anderson RM,van Stijn TC,Kristjánsson T,Clarke SM

    更新日期:2015-12-09 00:00:00

  • Identification and analysis of long non-coding RNAs and mRNAs in chicken macrophages infected with avian infectious bronchitis coronavirus.

    abstract:BACKGROUND:Avian infectious bronchitis virus (IBV) is a gamma coronavirus that severely affects the poultry industry worldwide. Long non-coding RNAs (lncRNAs), a subset of non-coding RNAs with a length of more than 200 nucleotides, have been recently recognized as pivotal factors in the pathogenesis of viral infections...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-07359-3

    authors: Li H,Cui P,Fu X,Zhang L,Yan W,Zhai Y,Lei C,Wang H,Yang X

    更新日期:2021-01-20 00:00:00

  • Application of dissociation curve analysis to radiation hybrid panel marker scoring: generation of a map of river buffalo (B. bubalis) chromosome 20.

    abstract:BACKGROUND:Fluorescence of dyes bound to double-stranded PCR products has been utilized extensively in various real-time quantitative PCR applications, including post-amplification dissociation curve analysis, or differentiation of amplicon length or sequence composition. Despite the current era of whole-genome sequenc...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-9-544

    authors: Kochan KJ,Amaral ME,Agarwala R,Schäffer AA,Riggs PK

    更新日期:2008-11-17 00:00:00

  • Selenium toxicity but not deficient or super-nutritional selenium status vastly alters the transcriptome in rodents.

    abstract:BACKGROUND:Protein and mRNA levels for several selenoproteins, such as glutathione peroxidase-1 (Gpx1), are down-regulated dramatically by selenium (Se) deficiency. These levels in rats increase sigmoidally with increasing dietary Se and reach defined plateaus at the Se requirement, making them sensitive biomarkers for...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-26

    authors: Raines AM,Sunde RA

    更新日期:2011-01-12 00:00:00

  • Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model.

    abstract:BACKGROUND:Tumor angiogenesis is a highly regulated process involving intercellular communication as well as the interactions of multiple downstream signal transduction pathways. Disrupting one or even a few angiogenesis pathways is often insufficient to achieve sustained therapeutic benefits due to the complexity of a...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-9-264

    authors: Chen Y,Wei T,Yan L,Lawrence F,Qian HR,Burkholder TP,Starling JJ,Yingling JM,Shou J

    更新日期:2008-06-02 00:00:00

  • Medicago truncatula transporter database: a comprehensive database resource for M. truncatula transporters.

    abstract:BACKGROUND:Medicago truncatula has been chosen as a model species for genomic studies. It is closely related to an important legume, alfalfa. Transporters are a large group of membrane-spanning proteins. They deliver essential nutrients, eject waste products, and assist the cell in sensing environmental conditions by f...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-60

    authors: Miao Z,Li D,Zhang Z,Dong J,Su Z,Wang T

    更新日期:2012-02-06 00:00:00

  • Yes, we can use it: a formal test on the accuracy of low-pass nanopore long-read sequencing for mitophylogenomics and barcoding research using the Caribbean spiny lobster Panulirus argus.

    abstract:BACKGROUND:Whole mitogenomes or short fragments (i.e., 300-700 bp of the cox1 gene) are the markers of choice for revealing within- and among-species genealogies. Protocols for sequencing and assembling mitogenomes include 'primer walking' or 'long PCR' followed by Sanger sequencing or Illumina short-read low-coverage ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-07292-5

    authors: Baeza JA

    更新日期:2020-12-09 00:00:00

  • RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stress.

    abstract:BACKGROUND:The Pacific oyster Crassostrea gigas is an important fishery resource that is sensitive to temperature fluctuations. Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. In other animals, h...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6003-8

    authors: Liu Y,Li L,Huang B,Wang W,Zhang G

    更新日期:2019-08-08 00:00:00

  • The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responses.

    abstract:BACKGROUND:Soybean (Glycine max (L. Merr.)) resistance to any population of Heterodera glycines (I.), or Fusarium virguliforme (Akoi, O'Donnell, Homma & Lattanzi) required a functional allele at Rhg1/Rfs2. H. glycines, the soybean cyst nematode (SCN) was an ancient, endemic, pest of soybean whereas F. virguliforme caus...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-13-368

    authors: Srour A,Afzal AJ,Blahut-Beatty L,Hemmati N,Simmonds DH,Li W,Liu M,Town CD,Sharma H,Arelli P,Lightfoot DA

    更新日期:2012-08-02 00:00:00

  • Nonlinear transcriptomic response to dietary fat intake in the small intestine of C57BL/6J mice.

    abstract:BACKGROUND:A high caloric diet, in conjunction with low levels of physical activity, promotes obesity. Many studies are available regarding the relation between dietary saturated fats and the etiology of obesity, but most focus on liver, muscle and white adipose tissue. Furthermore, the majority of transcriptomic studi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2424-9

    authors: Nyima T,Müller M,Hooiveld GJ,Morine MJ,Scotti M

    更新日期:2016-02-09 00:00:00

  • SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population.

    abstract:BACKGROUND:Progress in genetics and breeding in pea still suffers from the limited availability of molecular resources. SNP markers that can be identified through affordable sequencing processes, without the need for prior genome reduction or a reference genome to assemble sequencing data would allow the discovery and ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-2447-2

    authors: Boutet G,Alves Carvalho S,Falque M,Peterlongo P,Lhuillier E,Bouchez O,Lavaud C,Pilet-Nayel ML,Rivière N,Baranger A

    更新日期:2016-02-18 00:00:00

  • Mining statistically-solid k-mers for accurate NGS error correction.

    abstract:BACKGROUND:NGS data contains many machine-induced errors. The most advanced methods for the error correction heavily depend on the selection of solid k-mers. A solid k-mer is a k-mer frequently occurring in NGS reads. The other k-mers are called weak k-mers. A solid k-mer does not likely contain errors, while a weak k-...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5272-y

    authors: Zhao L,Xie J,Bai L,Chen W,Wang M,Zhang Z,Wang Y,Zhao Z,Li J

    更新日期:2018-12-31 00:00:00

  • A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection.

    abstract:BACKGROUND:Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. METHODS:To overcome these limitations, we developed a novel RoV capture and genome sequencing method combin...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-3714-6

    authors: Dung TTN,Duy PT,Sessions OM,Sangumathi UK,Phat VV,Tam PTT,To NTN,Phuc TM,Hong Chau TT,Chau NNM,Minh NN,Thwaites GE,Rabaa MA,Baker S

    更新日期:2017-04-24 00:00:00

  • Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis.

    abstract:BACKGROUND:The Xklp2 (TPX2) proteins belong to the microtubule-associated (MAP) family of proteins. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist with different microtubule functions, for example, maintenance of cell morphology ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-016-3303-0

    authors: Du P,Kumar M,Yao Y,Xie Q,Wang J,Zhang B,Gan S,Wang Y,Wu AM

    更新日期:2016-11-24 00:00:00

  • Genomic diversity dynamics in conserved chicken populations are revealed by genome-wide SNPs.

    abstract:BACKGROUND:Maintaining maximum genetic diversity and preserving breed viability in conserved populations necessitates the rigorous evaluation of conservation schemes. Three chicken breeds (Baier Yellow Chicken (BEC), Beijing You Chicken (BYC) and Langshan Chicken (LSC)) are currently in conservation programs in China. ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-4973-6

    authors: Zhang M,Han W,Tang H,Li G,Zhang M,Xu R,Liu Y,Yang T,Li W,Zou J,Wu K

    更新日期:2018-08-09 00:00:00

  • Genomic tools for durum wheat breeding: de novo assembly of Svevo transcriptome and SNP discovery in elite germplasm.

    abstract:BACKGROUND:The tetraploid durum wheat (Triticum turgidum L. ssp. durum Desf. Husnot) is an important crop which provides the raw material for pasta production and a valuable source of genetic diversity for breeding hexaploid wheat (Triticum aestivum L.). Future breeding efforts to enhance yield potential and climate re...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-5645-x

    authors: Vendramin V,Ormanbekova D,Scalabrin S,Scaglione D,Maccaferri M,Martelli P,Salvi S,Jurman I,Casadio R,Cattonaro F,Tuberosa R,Massi A,Morgante M

    更新日期:2019-04-10 00:00:00

  • Complete chloroplast genome sequence of Barleria prionitis, comparative chloroplast genomics and phylogenetic relationships among Acanthoideae.

    abstract:BACKGROUND:The plastome of medicinal and endangered species in Kingdom of Saudi Arabia, Barleria prionitis was sequenced. The plastome was compared with that of seven Acanthoideae species in order to describe the plastome, spot the microsatellite, assess the dissimilarities within the sampled plastomes and to infer the...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-020-06798-2

    authors: Alzahrani DA,Yaradua SS,Albokhari EJ,Abba A

    更新日期:2020-06-06 00:00:00

  • Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection.

    abstract:BACKGROUND:The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we un...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-257

    authors: Baton LA,Robertson A,Warr E,Strand MR,Dimopoulos G

    更新日期:2009-06-05 00:00:00

  • Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum.

    abstract:BACKGROUND:It has been shown that nearly a quarter of the initial predicted gene models in the Plasmodium falciparum genome contain errors. Although there have been efforts to obtain complete cDNA sequences to correct the errors, the coverage of cDNA sequences on the predicted genes is still incomplete, and many gene m...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-12-587

    authors: López-Barragán MJ,Lemieux J,Quiñones M,Williamson KC,Molina-Cruz A,Cui K,Barillas-Mury C,Zhao K,Su XZ

    更新日期:2011-11-30 00:00:00

  • Expression profile of genes regulated by activity of the Na-H exchanger NHE1.

    abstract:BACKGROUND:In mammalian cells changes in intracellular pH (pHi), which are predominantly controlled by activity of plasma membrane ion exchangers, regulate a diverse range of normal and pathological cellular processes. How changes in pHi affect distinct cellular processes has primarily been determined by evaluating pro...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-5-46

    authors: Putney LK,Barber DL

    更新日期:2004-07-16 00:00:00

  • Does replication groups scoring reduce false positive rate in SNP interaction discovery?

    abstract:BACKGROUND:Computational methods that infer single nucleotide polymorphism (SNP) interactions from phenotype data may uncover new biological mechanisms in non-Mendelian diseases. However, practical aspects of such analysis face many problems. Present experimental studies typically use SNP arrays with hundreds of thousa...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-58

    authors: Toplak M,Curk T,Demsar J,Zupan B

    更新日期:2010-01-22 00:00:00

  • Papain-like cysteine proteases in Carica papaya: lineage-specific gene duplication and expansion.

    abstract:BACKGROUND:Papain-like cysteine proteases (PLCPs), a large group of cysteine proteases structurally related to papain, play important roles in plant development, senescence, and defense responses. Papain, the first cysteine protease whose structure was determined by X-ray crystallography, plays a crucial role in protec...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-017-4394-y

    authors: Liu J,Sharma A,Niewiara MJ,Singh R,Ming R,Yu Q

    更新日期:2018-01-06 00:00:00

  • A comparative approach to elucidate chloroplast genome replication.

    abstract:BACKGROUND:Electron microscopy analyses of replicating chloroplast molecules earlier predicted bidirectional Cairns replication as the prevalent mechanism, perhaps followed by rounds of a rolling circle mechanism. This standard model is being challenged by the recent proposition of homologous recombination-mediated rep...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-10-237

    authors: Krishnan NM,Rao BJ

    更新日期:2009-05-20 00:00:00

  • DEBrowser: interactive differential expression analysis and visualization tool for count data.

    abstract:BACKGROUND:Sequencing data has become a standard measure of diverse cellular activities. For example, gene expression is accurately measured by RNA sequencing (RNA-Seq) libraries, protein-DNA interactions are captured by chromatin immunoprecipitation sequencing (ChIP-Seq), protein-RNA interactions by crosslinking immun...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5362-x

    authors: Kucukural A,Yukselen O,Ozata DM,Moore MJ,Garber M

    更新日期:2019-01-05 00:00:00

  • Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti.

    abstract:BACKGROUND:One of the key characters of social insects is the division of labor, in which different tasks are allocated to various castes. In termites, one of the representative groups of social insects, morphological differences as well as behavioral differences can be recognized among castes. However, very little is ...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-11-314

    authors: Ishikawa Y,Okada Y,Ishikawa A,Miyakawa H,Koshikawa S,Miura T

    更新日期:2010-05-20 00:00:00

  • Exclusivity offers a sound yet practical species criterion for bacteria despite abundant gene flow.

    abstract:BACKGROUND:The question of whether bacterial species objectively exist has long divided microbiologists. A major source of contention stems from the fact that bacteria regularly engage in horizontal gene transfer (HGT), making it difficult to ascertain relatedness and draw boundaries between taxa. A natural way to defi...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-018-5099-6

    authors: Wright ES,Baum DA

    更新日期:2018-10-03 00:00:00

  • Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation.

    abstract:BACKGROUND:Malaria pathogenesis relies on sexual gametocyte forms of the malaria parasite to be transmitted between the infected human and the mosquito host but the molecular mechanisms controlling gametocytogenesis remains poorly understood. Here we provide a high-resolution transcriptome of Plasmodium falciparum as i...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/s12864-019-6322-9

    authors: van Biljon R,van Wyk R,Painter HJ,Orchard L,Reader J,Niemand J,Llinás M,Birkholtz LM

    更新日期:2019-12-03 00:00:00

  • Tests for differential gene expression using weights in oligonucleotide microarray experiments.

    abstract:BACKGROUND:Microarray data analysts commonly filter out genes based on a number of ad hoc criteria prior to any high-level statistical analysis. Such ad hoc approaches could lead to conflicting conclusions with no clear guidance as to which method is most likely to be reproducible. Furthermore, the number of tests perf...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-7-33

    authors: Hu P,Beyene J,Greenwood CM

    更新日期:2006-02-22 00:00:00

  • Genomic meta-analysis of growth factor and integrin pathways in chronic kidney transplant injury.

    abstract:BACKGROUND:Chronic Allograft Nephropathy (CAN) is a clinical entity of progressive kidney transplant injury. The defining histology is tubular atrophy with interstitial fibrosis (IFTA). Using a meta-analysis of microarrays from 84 kidney transplant biopsies, we revealed growth factor and integrin adhesion molecule path...

    journal_title:BMC genomics

    pub_type: 杂志文章,meta分析

    doi:10.1186/1471-2164-14-275

    authors: Dosanjh A,Robison E,Mondala T,Head SR,Salomon DR,Kurian SM

    更新日期:2013-04-23 00:00:00

  • Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes.

    abstract:BACKGROUND:The phylum Platyhelminthes (flatworms) contains an important group of bilaterian organisms responsible for many debilitating and chronic infectious diseases of human and animal populations inhabiting the planet today. In addition to their biomedical and veterinary relevance, some platyhelminths are also freq...

    journal_title:BMC genomics

    pub_type: 杂志文章

    doi:10.1186/1471-2164-14-462

    authors: Geyer KK,Chalmers IW,Mackintosh N,Hirst JE,Geoghegan R,Badets M,Brophy PM,Brehm K,Hoffmann KF

    更新日期:2013-07-09 00:00:00