The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.


BACKGROUND:Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to cold stress in this study. METHODS:The second to fourth fully expanded leaves from up to down were harvested at different stress time points forthe transmission electron microscope (TEM) observation. Physiological characteristics measurement included the relative electrolyte leakage (REL), SOD activity assay, soluble sugar content, and Chlorophyll fluorescence parameter measurement. For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads. To identify the differentially expressed protein, leaves of plants under 0, 6, 12, 24, 48 and 72 h cold stress wereharvested for proteomic analysis. Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data. RESULTS:Results showed that at the beginning of cold stress, respiratory metabolism was decreased and the transportation and hydrolysis of photosynthetic products was inhibited, leading to an accumulation of starch in the chloroplasts. Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism. Although the transcriptional level of a large number of genes has been restored after 12 h, sustained cold stress brought more serious injury to the leaf cells, including the sharp rise of the relative electrolyte leakage, the declined Fv/Fm value, swelled chloroplast and the disintegrated membrane system. CONCLUSION:The starch accumulation and the photoinhibition might be the main adaptive mechanism of the paper mulberry responded to cold stress. Most of important, enhancing the transport and hydrolysis of photosynthetic products could be the potential targets for improving the cold tolerance of the paper mulberry.


BMC Genomics


BMC genomics


Peng X,Teng L,Yan X,Zhao M,Shen S




Has Abstract


2015-11-05 00:00:00










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    pub_type: 杂志文章


    authors: Sharma G,Parales R,Singer M

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    abstract:BACKGROUND:There is strong but mostly circumstantial evidence that genetic factors modulate the severity of influenza infection in humans. Using genetically diverse but fully inbred strains of mice it has been shown that host sequence variants have a strong influence on the severity of influenza A disease progression. ...

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    authors: McDonald TR,Mueller O,Dietrich FS,Lutzoni F

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    authors: Cazares A,Mendoza-Hernández G,Guarneros G

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    authors: Beatty M,Guduric-Fuchs J,Brown E,Bridgett S,Chakravarthy U,Hogg RE,Simpson DA

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    authors: Tu Q,Lin L

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    authors: Fumagalli D,Blanchet-Cohen A,Brown D,Desmedt C,Gacquer D,Michiels S,Rothé F,Majjaj S,Salgado R,Larsimont D,Ignatiadis M,Maetens M,Piccart M,Detours V,Sotiriou C,Haibe-Kains B

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    authors: He S,Liang XF,Sun J,Li L,Yu Y,Huang W,Qu CM,Cao L,Bai XL,Tao YX

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    abstract:BACKGROUND:The beta-defensin gene cluster (DEFB) at chromosome 8p23.1 is one of the most copy number (CN) variable regions of the human genome. Whereas individual DEFB CNs have been suggested as independent genetic risk factors for several diseases (e.g. psoriasis and Crohn's disease), the role of multisite sequence va...

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    authors: Taudien S,Groth M,Huse K,Petzold A,Szafranski K,Hampe J,Rosenstiel P,Schreiber S,Platzer M

    更新日期:2010-04-19 00:00:00

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    abstract:BACKGROUND:Democratising the growing body of whole genome sequencing data available for Triticum aestivum (bread wheat) has been impeded by the lack of a genome reference and the large computational requirements for analysing these data sets. RESULTS:DAWN (Diversity Among Wheat geNomes) integrates data from the T. aes...

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    authors: Watson-Haigh NS,Suchecki R,Kalashyan E,Garcia M,Baumann U

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    authors: Sambrook JG,Figueroa F,Beck S

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    pub_type: 杂志文章


    authors: Krishnan K,Ren Z,Losada L,Nierman WC,Lu LJ,Askew DS

    更新日期:2014-02-25 00:00:00

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    abstract:BACKGROUND:According to history, in the pre-Hispanic period, during the conquest and Inka expansion in Ecuador, many Andean families of the Cañar region would have been displaced to several places of Tawantinsuyu, including Kañaris, a Quechua-speaking community located at the highlands of the Province of Ferreñafe, Lam...

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    authors: Sandoval JR,Lacerda DR,Jota MMS,Robles-Ruiz P,Danos P,Paz-Y-Miño C,Wells S,Santos FR,Fujita R

    更新日期:2020-09-10 00:00:00

  • Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells.

    abstract:BACKGROUND:S. aureus is one of the main pathogens responsible for the intra-mammary infection in dairy ruminants. Although much work has been carried out to understand the complex physiological and cellular events that occur in the mammary gland in response to S. aureus, the protective mechanisms are still poorly under...

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    authors: Cremonesi P,Capoferri R,Pisoni G,Del Corvo M,Strozzi F,Rupp R,Caillat H,Modesto P,Moroni P,Williams JL,Castiglioni B,Stella A

    更新日期:2012-10-09 00:00:00

  • The odds of duplicate gene persistence after polyploidization.

    abstract:BACKGROUND:Gene duplication is an important biological phenomenon associated with genomic redundancy, degeneration, specialization, innovation, and speciation. After duplication, both copies continue functioning when natural selection favors duplicated protein function or expression, or when mutations make them functio...

    journal_title:BMC genomics

    pub_type: 杂志文章


    authors: Chain FJ,Dushoff J,Evans BJ

    更新日期:2011-12-12 00:00:00

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    pub_type: 杂志文章


    authors: Ciric M,Moon CD,Leahy SC,Creevey CJ,Altermann E,Attwood GT,Rakonjac J,Gagic D

    更新日期:2014-05-12 00:00:00

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    pub_type: 杂志文章,meta分析


    authors: Calura E,Cagnin S,Raffaello A,Laveder P,Lanfranchi G,Romualdi C

    更新日期:2008-12-23 00:00:00

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    abstract:BACKGROUND:Malaria is the most important parasitic disease in the world with approximately two million people dying every year, mostly due to Plasmodium falciparum infection. During its complex life cycle in the Anopheles vector and human host, the parasite requires the coordinated and modulated expression of diverse s...

    journal_title:BMC genomics

    pub_type: 杂志文章


    authors: Bischoff E,Vaquero C

    更新日期:2010-01-15 00:00:00

  • Identification of a strawberry flavor gene candidate using an integrated genetic-genomic-analytical chemistry approach.

    abstract:BACKGROUND:There is interest in improving the flavor of commercial strawberry (Fragaria × ananassa) varieties. Fruit flavor is shaped by combinations of sugars, acids and volatile compounds. Many efforts seek to use genomics-based strategies to identify genes controlling flavor, and then designing durable molecular mar...

    journal_title:BMC genomics

    pub_type: 杂志文章


    authors: Chambers AH,Pillet J,Plotto A,Bai J,Whitaker VM,Folta KM

    更新日期:2014-04-17 00:00:00

  • High-throughput comparison of gene fitness among related bacteria.

    abstract:BACKGROUND:The contribution of a gene to the fitness of a bacterium can be assayed by whether and to what degree the bacterium tolerates transposon insertions in that gene. We use this fact to compare the fitness of syntenic homologous genes among related Salmonella strains and thereby reveal differences not apparent a...

    journal_title:BMC genomics

    pub_type: 杂志文章


    authors: Canals R,Xia XQ,Fronick C,Clifton SW,Ahmer BM,Andrews-Polymenis HL,Porwollik S,McClelland M

    更新日期:2012-05-30 00:00:00

  • Genome and Transcriptome sequence of Finger millet (Eleusine coracana (L.) Gaertn.) provides insights into drought tolerance and nutraceutical properties.

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    pub_type: 杂志文章


    authors: Hittalmani S,Mahesh HB,Shirke MD,Biradar H,Uday G,Aruna YR,Lohithaswa HC,Mohanrao A

    更新日期:2017-06-15 00:00:00

  • Gene expression profiling of Spodoptera frugiperda hemocytes and fat body using cDNA microarray reveals polydnavirus-associated variations in lepidopteran host genes transcript levels.

    abstract:BACKGROUND:Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray to analyze the gene transcription profile of the lepidopteran pest Spodoptera frugiperda in response to injection of the polydnavirus HdIV associated with the ichneumonid wasp...

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    pub_type: 杂志文章


    authors: Barat-Houari M,Hilliou F,Jousset FX,Sofer L,Deleury E,Rocher J,Ravallec M,Galibert L,Delobel P,Feyereisen R,Fournier P,Volkoff AN

    更新日期:2006-06-21 00:00:00