Abstract：
:Necrotizing enterocolitis (NEC) is a devastating gastrointestinal emergency of neonates. Epithelial tight junction (TJ) proteins, such as claudins, are essential for regulation and function of the intestinal barrier. Rho kinase (ROCK) affects cellular permeability and TJ regulation. We hypothesized that TJ protein changes would correlate with increased permeability in experimental NEC, and ROCK inhibitors would be protective against NEC by regulation of key claudin proteins. We tested this hypothesis using an in vivo rat pup model, an in vitro model of experimental NEC, and human intestinal samples from patients with and without NEC. Experimental NEC was induced in rats via hypoxia and bacteria-containing formula, and in Caco-2 cells by media inoculated with LPS. The expression of claudins was measured by gene and protein analysis. Experimental NEC in rat pups and Caco-2 cells had increased permeability compared to controls. Gene and protein expression of claudin 2 was increased in experimental NEC. Sub-cellular fractionation localized increased claudin 2 protein to the cytoskeleton. ROCK inhibition was associated with normalization of these alterations and decreased severity of experimental NEC. Co-immunoprecipitation of caveolin-1 with claudin 2 suggests that caveolin-1 may act as a shuttle for the internalization of claudin 2 seen in experimental NEC. In conclusion, NEC is associated with intestinal permeability and increased expression of claudin 2, increased binding of caveolin-1 and claudin 2, and increased trafficking of claudin 2 to the cytoskeleton.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ares G,Buonpane C,Sincavage J,Yuan C,Wood DR,Hunter CJdoi
10.1038/s41598-019-41442-4subject
Has Abstractpub_date
2019-03-21 00:00:00pages
4982issue
1issn
2045-2322pii
10.1038/s41598-019-41442-4journal_volume
9pub_type
杂志文章abstract：:We performed continuous recordings (May 2015 - January 2017) of hydraulic pressure and electrical conductivity of groundwater in the 190 m-long horizontal S13 borehole drilled next to the deep underground laboratories of Gran Sasso (LNGS-INFN), located in the core of the Gran Sasso carbonate aquifer (central Italy) at...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34444-1
更新日期：2018-10-29 00:00:00
abstract：:Based on the pro-drug principle, aspirin and eugenol were used to synthesize aspirin eugenol ester (AEE) by esterification reaction. In present study, the anti-atherosclerosis effects of AEE were investigated in hamsters with the utilization of metabonomic approach based on UPLC-Q-TOF/MS. Biochemical parameters and hi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11422-7
更新日期：2017-09-05 00:00:00
abstract：:Atropine eye drops and myopic retinal defocus each slow progression of myopia (short-sight). They also cause thickening of the choroid, and it has been suggested that the thickening is a precursor for reduced eye growth and slowed myopia progression. We investigated whether choroidal thickening due to optical defocus ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75342-9
更新日期：2020-10-27 00:00:00
abstract：:Childhood obesity is a risk factor for asthma, but the molecular mechanisms linking both remain elusive. Since obesity leads to chronic low-grade inflammation and affects metabolic signaling we hypothesized that postnatal hyperalimentation (pHA) induced by maternal high-fat-diet during lactation leads to early-onset o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24168
更新日期：2016-04-18 00:00:00
abstract：:Graphite's capacity of intercalating lithium in rechargeable batteries is limited (theoretically, 372 mAh g-1) due to low diffusion within commensurately-stacked graphene layers. Graphene foam with highly enriched incommensurately-stacked layers was grown and applied as an active electrode in rechargeable batteries. A...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39944
更新日期：2017-01-06 00:00:00
abstract：:The spatio-temporal distribution soil pH is critical for understanding the productivity and long-term sustainability of our agri-ecosystem. This study quantified the spatio-temporal distribution of paddy soil pH in Jiangxi province, China, and the potential driver of the change between 1980 and 2010. Data from the Soi...
journal_title：Scientific reports
pub_type： 历史文章,杂志文章
doi：10.1038/s41598-018-20873-5
更新日期：2018-02-09 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-58293-z
更新日期：2020-01-24 00:00:00
abstract：:Bismuth has long been a prototypical system for investigating phase transformations and melting at high pressure. Despite decades of experimental study, however, the lattice-level response of Bi to rapid (shock) compression and the relationship between structures occurring dynamically and those observed during slow (s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35260-3
更新日期：2018-11-16 00:00:00
abstract：:Medium chain fatty acids can activate the pro-inflammatory receptor GPR84 but so also can molecules related to 3,3'-diindolylmethane. 3,3'-Diindolylmethane and decanoic acid acted as strong positive allosteric modulators of the function of each other and analysis showed the affinity of 3,3'-diindolylmethane to be at l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18159-3
更新日期：2017-12-20 00:00:00
abstract：:CRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts, has proven to be very efficient with regards to mutation rates, but the precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway has seldom been achieved outside of the model organisms. Here, we succeed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79409-5
更新日期：2020-12-17 00:00:00
abstract：:Glypican-3 (GPC3) is a cell-surface heparan sulfate proteoglycan highly expressed in hepatocellular carcinoma (HCC). We have generated a group of high-affinity mouse monoclonal antibodies targeting GPC3. Here, we report the humanization and testing of these antibodies for clinical development. We compared the affinity...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33878
更新日期：2016-09-26 00:00:00
abstract：:The ability of human metaphase-II arrested eggs to activate following fertilisation declines with advancing maternal age. Egg activation is triggered by repetitive increases in intracellular Ca(2+) concentration ([Ca(2+)]i) in the ooplasm as a result of sperm-egg fusion. We therefore hypothesised that eggs from older ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19357
更新日期：2016-01-20 00:00:00
abstract：:Visceral adiposity is a risk factor for cardiovascular disorders, type 2 diabetes mellitus (T2D) and associated metabolic diseases. Sub-cutaneous fat is believed to be intrinsically different from visceral fat. To understand molecular mechanisms involved in metabolic advantages of fat transplantation, we studied a rat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00081
更新日期：2011-01-01 00:00:00
abstract：:It is frequently assumed that in the limit of vanishing cooling rate, the glass transition phenomenon becomes a thermodynamic transition at a temperature TK. However, with any finite cooling rate, the system falls out of equilibrium at temperatures near Tg(>TK), implying that the very existence of the putative thermod...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12577
更新日期：2015-07-24 00:00:00
abstract：:Recent studies have shown that ionic direct current (iDC) can modulate the vestibular system in-vivo, with potential benefits over conventional pulsed stimulation. In this study, the effects of iDC stimulation on vestibular nerve fiber firing rate was investigated using loose-patch nerve fiber recordings in the acutel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55045-6
更新日期：2019-12-12 00:00:00
abstract：:Somatostatin (SST)-positive interneurons in the anterior cingulate cortex (ACC) play important roles in neuronal diseases, memory and cognitive functions. However, their development in the ACC remains unclear. Using postnatal day 3 (P3) to P45 GIN mice, we found that most of the intrinsic membrane properties of SST in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28137
更新日期：2016-06-20 00:00:00
abstract：:CRISPR-Cas9 is a powerful new tool for genome editing, but this technique creates mosaic mutations that affect the efficiency and precision of its ability to edit the genome. Reducing mosaic mutations is particularly important for gene therapy and precision genome editing. Although the mechanisms underlying the CRSIPR...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42081
更新日期：2017-02-03 00:00:00
abstract：:Multiferroics, where two or more ferroic order parameters coexist, is one of the hottest fields in condensed matter physics and materials science. To search multiferroics, currently most researches are focused on frustrated magnets, which usually have complicated magnetic structure and low magnetic ordering temperatur...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22743
更新日期：2016-03-09 00:00:00
abstract：:Preventing the protein-protein interaction of the cellular chromatin binding protein Lens Epithelium-Derived Growth Factor (LEDGF) and human immunodeficiency virus (HIV) integrase is an important possible strategy for anti-viral treatment for AIDS. We have used Intracellular Antibody Capture technology to isolate a si...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16742-2
更新日期：2017-12-04 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep46667. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/srep46924
更新日期：2017-12-22 00:00:00
abstract：:The role of the actin cytoskeleton in relation to mitochondria function and dynamics is only recently beginning to be recognized. Myo19 is an actin-based motor that is bound to the outer mitochondrial membrane and promotes the localization of mitochondria to filopodia in response to glucose starvation. However, how gl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11002-9
更新日期：2017-09-14 00:00:00
abstract：:Networks in nature are often formed within a spatial domain in a dynamical manner, gaining links and nodes as they develop over time. Motivated by the growth and development of neuronal networks, we propose a class of spatially-based growing network models and investigate the resulting statistical network properties a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07047
更新日期：2014-11-14 00:00:00
abstract：:Omega-3 fatty acids, and specifically docosahexaenoic acid (DHA), are important and essential nutrients for human health. Thraustochytrids are recognised as commercial strains for nutraceuticals production, they are group of marine oleaginous microorganisms capable of co-synthesis of DHA and other valuable carotenoids...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58728-7
更新日期：2020-02-06 00:00:00
abstract：:Bacteria are able to de-epoxidize or epimerize deoxynivalenol (DON), a mycotoxin, to deepoxy-deoxynivalenol (deepoxy-DON or DOM-1) or 3-epi-deoxynivalenol (3-epi-DON), respectively. Using different approaches, the intestinal toxicity of 3 molecules was compared and the molecular basis for the reduced toxicity investig...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29105
更新日期：2016-07-06 00:00:00
abstract：:CVB3 is a common human pathogen to be highly lethal to newborns and causes viral myocarditis and pancreatitis in adults. However, there is no vaccine available for clinical use. CVB3 capsid protein VP1 is an immunodominant structural protein, containing several B- and T-cell epitopes. However, immunization of mice wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41485
更新日期：2017-02-02 00:00:00
abstract：:Future climate change has the potential to alter the distribution and prevalence of plant pathogens, which may have significant implications for both agricultural crops and natural plant communities. However, there are few long-term datasets against which modelled predictions of pathogen responses to climate change ca...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35299-2
更新日期：2018-11-21 00:00:00
abstract：:The taxonomic composition of egg-associated microbial communities can play a crucial role in the development of fish embryos. In response, hosts increasingly influence the composition of their associated microbial communities during embryogenesis, as concluded from recent field studies and laboratory experiments. Howe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17084
更新日期：2015-11-27 00:00:00
abstract：:There are three sex ratio strategies (SRS) in nature-male-biased sex ratio, female-biased sex ratio and, equal sex ratio. It was R. A. Fisher who first explained why most species in nature display a sex ratio of ½. Consequent SRS theories such as Hamilton's local mate competition (LMC) and Clark's local resource compe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39807
更新日期：2016-12-23 00:00:00
abstract：:Psychiatric disorders have been widely reported to be associated with systemic inflammation upregulation and adiposity. However, there are no data that link adipose tissue inflammation to these mental disorders. The analysis of adipokines and inflammation-related markers in adipose tissue could help to elucidate the p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35759-9
更新日期：2018-11-30 00:00:00
abstract：:The COVID-19 pandemic caused by the SARS-CoV-2 requires a fast development of antiviral drugs. SARS-CoV-2 viral main protease (Mpro, also called 3C-like protease, 3CLpro) is a potential target for drug design. Crystal and co-crystal structures of the SARS-CoV-2 Mpro have been solved, enabling the rational design of in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77794-5
更新日期：2020-11-30 00:00:00
