Abstract:
:Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process is particularly important for proper neuronal function; however, little is known about how RNA editing is dynamically regulated between the many functionally distinct neuronal populations of the brain. Here, we present a spatial RNA editing map in the Drosophila brain and show that different neuronal populations possess distinct RNA editing signatures. After purifying and sequencing RNA from genetically marked groups of neuronal nuclei, we identified a large number of editing sites and compared editing levels in hundreds of transcripts across nine functionally different neuronal populations. We found distinct editing repertoires for each population, including sites in repeat regions of the transcriptome and differential editing in highly conserved and likely functional regions of transcripts that encode essential neuronal genes. These changes are site-specific and not driven by changes in Adar expression, suggesting a complex, targeted regulation of editing levels in key transcripts. This fine-tuning of the transcriptome between different neurons by RNA editing may account for functional differences between distinct populations in the brain.
journal_name
Proc Natl Acad Sci U S Aauthors
Sapiro AL,Shmueli A,Henry GL,Li Q,Shalit T,Yaron O,Paas Y,Billy Li J,Shohat-Ophir Gdoi
10.1073/pnas.1811768116subject
Has Abstractpub_date
2019-02-05 00:00:00pages
2318-2327issue
6eissn
0027-8424issn
1091-6490pii
1811768116journal_volume
116pub_type
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