Indirubin-3'-oxime suppresses human cholangiocarcinoma through cell-cycle arrest and apoptosis.


:Cholangiocarcinoma (CCA) is one of the most serious of all cancers and a major public health problem. CCA is an extremely invasive cancer, and the survival rate for CCA patients is only 24 months after diagnosis. Although surgery and chemotherapy can extend the survival rate to 5 years, < 20-40% of CCA patients will survive this long; therefore, it is crucial to discover an effective chemotherapeutic agent for CCA. Indirubin-3'-oxime (I3O), a derivative of indirubin, has been shown to suppress cell proliferation and induce cell-cycle arrest and cell apoptosis in various human cancers. In this study, four human CCA cell lines-NOZ, HuCCT1, OCUG-1, and OZ-were used to evaluate the anticancer properties of I3O. Cell viability, cell-cycle arrest, and apoptosis were assessed using Western blotting, immunofluorescence, and flow cytometry analysis. The data show that I3O treatment can inhibit cell proliferation and induce cell-cycle arrest, and caspase-dependent apoptosis in CCA cells. These findings suggest that I3O could suppress tumor growth by regulating the cell cycle and inducing apoptosis, and is a potential therapeutic agent for treating human CCA.


Eur J Pharmacol


Lee MY,Li YZ,Huang KJ,Huang HC,Lin CY,Lee YR




Has Abstract


2018-11-15 00:00:00












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    abstract::High glucose is one of the possible causes for osteoporosis and fracture in diabetes mellitus. Our previous study showed that silibinin can increase osteogenic effect by stimulating osteogenic genes expression in human bone marrow stem cells (hBMSCs). However, no study has yet investigated the effect of silibinin on o...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Ying X,Chen X,Liu H,Nie P,Shui X,Shen Y,Yu K,Cheng S

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    abstract::This study assessed the effect of an uncontrollable stressor on the preference for a palatable solution (sucrose 1%), and on the preference for a context associated with a single administration of D-amphetamine (3 mg/kg i.p.) by means of the conditioning place preference test. We also evaluated the effect of prior nal...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Zurita A,Murúa S,Molina V

    更新日期:1996-03-28 00:00:00

  • Tolterodine--a new bladder-selective antimuscarinic agent.

    abstract::Tolterodine is a new muscarinic receptor antagonist intended for the treatment of urinary urge incontinence and other symptoms related to an overactive bladder. The aim of the present study was to compare the antimuscarinic properties of tolterodine with those of oxybutynin, in vitro and in vivo. Tolterodine effective...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Nilvebrant L,Andersson KE,Gillberg PG,Stahl M,Sparf B

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  • N-methyl-D-aspartate receptors involved in morphine-induced hyperalgesia in sensitized mice.

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    authors: Ahmadi S,Golbaghi H,Azizbeigi R,Esmailzadeh N

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  • Binding of bromine-substituted analogs of methylphenidate to monoamine transporters.

    abstract::We synthesized the o-, m- and p-bromo derivatives of dl-threo-methylphenidate from the corresponding bromophenylacetonitriles by modification of the literature synthesis of methylphenidate (Panizzon, Helv. Chim. Acta 1944, 27, 1748). In in vitro binding assays all three dl-threo bromo compounds had higher affinities t...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Pan D,Gatley SJ,Dewey SL,Chen R,Alexoff DA,Ding YS,Fowler JS

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    abstract::The therapeutic potential of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated in rats exposed to ischemia/reperfusion liver injury. Ischemia was induced by clamping the pedicle of the left hepatic lobe for 30 min, and cannabidiol (5mg/kg, i.v.) was given 1h following the procedure and eve...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Fouad AA,Jresat I

    更新日期:2011-11-16 00:00:00

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    authors: Sebens JB,Middelveld RJ,Koch T,Ter Horst GJ,Korf J

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Markstein R

    更新日期:1982-12-24 00:00:00

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Fatehi-Hassanabad Z,Imen-Shahidi M,Fatehi M,Farrokhfall K,Parsaeei H

    更新日期:2006-03-27 00:00:00

  • Improvement of postischemic contractile dysfunction of dog heart by MCI-154, a novel cardiotonic agent.

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Abe Y,Kitada Y,Narimatsu A,Tobe A

    更新日期:1991-01-10 00:00:00

  • L-Arginine supplementation improves insulin sensitivity and beta cell function in the offspring of diabetic rats through AKT and PDX-1 activation.

    abstract::Maternal hyperglycemia can result in defects in glucose metabolism and pancreatic β-cell function in offspring. The purpose of this study was to evaluate the impact of maternal diabetes mellitus on pancreatic islets, muscle and adipose tissue of the offspring, with or without oral l-Arginine supplementation. The induc...

    journal_title:European journal of pharmacology

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    authors: Carvalho DS,Diniz MM,Haidar AA,Cavanal MF,da Silva Alves E,Carpinelli AR,Gil FZ,Hirata AE

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    journal_title:European journal of pharmacology

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    authors: Ui H,Andoh T,Lee JB,Nojima H,Kuraishi Y

    更新日期:2006-01-13 00:00:00

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    abstract::Adrenomedullin administered peripherally in the rabbit (at doses of 1.25, 2.5 and 5 microg/kg ) caused a dose-dependent conjunctival hyperemia accompanied by an increase of inflammatory cell number and prostaglandin E(2) concentration in the aqueous humor, and of uveal vascular response and myeloperoxidase activity. T...

    journal_title:European journal of pharmacology

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Rónai AZ

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    authors: Pawlik T,Konturek PC,Konturek JW,Konturek SJ,Brzozowski T,Cześnikiewicz M,Plonka M,Bielanski W,Areny H

    更新日期:2002-08-02 00:00:00

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    journal_title:European journal of pharmacology

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    authors: Koeberle A,Siemoneit U,Northoff H,Hofmann B,Schneider G,Werz O

    更新日期:2009-04-17 00:00:00

  • A role for protein kinase C in the attenuated response to 5-hydroxytryptamine in aortas from streptozotocin-diabetic rats.

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: James GM,Hodgson WC

    更新日期:1997-03-12 00:00:00

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Hatanaka K,Yatsugi S,Yamaguchi T

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    journal_title:European journal of pharmacology

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    authors: Yoshinaga M,Sunagawa M,Shimada S,Nakamura M,Murayama S,Kosugi T

    更新日期:2003-02-07 00:00:00

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    journal_title:European journal of pharmacology

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    authors: Maletínská L,Slaninová J,Kunes J,Zelezná B

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Janowsky A,Schweri MM,Berger P,Long R,Skolnick P,Paul SM

    更新日期:1985-01-22 00:00:00

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Wiley JL

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    journal_title:European journal of pharmacology

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    authors: Greenfeder S,Sehring S,McHugh N,Corboz M,Rivelli M,Anthes JC,Billah M,Egan RW,Chapman RW

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: El-Medany A,Hagar HH,Moursi M,At Muhammed R,El-Rakhawy FI,El-Medany G

    更新日期:2005-02-10 00:00:00

  • Cannabidiol selectively inhibits the contraction of rat small resistance arteries: Possible role for CGRP and voltage-gated calcium channels.

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    authors: Mazeh AC,Angus JA,Wright CE

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: D'Orléans-Juste P,Claing A,Télémaque S,Warner TD,Regoli D

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: De Gennaro Colonna V,Rossoni G,Rigamonti A,Bonomo S,Manfredi B,Berti F,Muller E

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    authors: Ding Y,Zhao L,Mei H,Zhang SL,Huang ZH,Duan YY,Ye P

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Singh L,Field MJ,Hughes J,Kuo BS,Suman-Chauhan N,Tuladhar BR,Wright DS,Naylor RJ

    更新日期:1997-02-26 00:00:00